Immuno Tools

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Your source for innovations.com

Hotline 04 70 03 73 06interbiotech@ interchim.com


Interchim provides more than 100 000 primary antibodies and all secondary and accessory reagents to perform your immunodetections in ELISA, Blotting, FCM or microscopy techniques. This includes :» Our high quality II antibodies labeled by HRP, Alkaline Phosphatase, Biotin and our great FluoProbes dyes» Fluograde microplates (FPlyte)» Ultimate sensitivity HRP substrates (TMB plus)» Remarkable saturating agents (Seablock).


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Tél 33 (0)4 70 03 88 55 - Fax 33 (0)4 70 03 82 60 - www.interchim.com

Hotline 04 70 03 73 06interbiotech@ interchim.com Prim'Ab specific topics

Introduction

See Prim'Ab full table in chap.E

The use of primary antibodies (Prim’Ab) as research tools is, in many ways, responsible for much of the progress seen in modern biological research. These antibodies, specific of target antigens, are a first step to probes samples in various detection. They can also be used to immobilize antigens on supports. Secondary antibodies are used in a second step to detect and amplify Prim’Ab signal by colour or light emission as fluorescence or chemiluminescence.

INTERCHIM is proud to offer one of the largest choice of high quality primary antibodies (more than 100 000 items) from the most famost manufacturers all over the world.

The following pages present a list of :» Interchim’s reliable suppliers for primary antibodies» Interesting and unique antibody technical features (such as Rabbit monoclonals...)» Anti-tags and labels antibodies» Loading control antibodies» Phosphospecific antibodies

One of the earliest use for antibodies, came in the early part of the 20th Century when Karl Lands-teiner develop a serological test for identifying different blood groups (ABO system for typing blood). Today antibodies are used as versatile reagents for an extremely wide range of assays, in technics as varied as screening phage libraries, biosensors, affinity purifications, and conventional immunoassays including ELISA, blotting, flow cytometry, agglutinations...Because of these multiple uses, a wide range of antibodies is needed (regarding antigen-specificity, binding affinity, and format/purity). Fortunately, a large number of companies now provides antibodies for researchers. This means that each individual lab no longer has to go through the hassle of generating, purifying and testing their own antibodies. However it is often difficult to find out the antibody companies, and the best supplier.

Some History...

A large selection of our antibodies and antigens are listed in chapter E. This table (by alphanumerical order of specificities) displays key information: species cross reactivity, host and quantity, compatible immunotechniques.

These listings cannot be exhaustive as there are too many items available. Please visit our website and Prim’Ab search engine to get the most updated list.

Prim’Ab search engineOver 100 000 primary antibodies available online with our powerful Prim'Ab search. Prim'Ab search offers an easy way to compare antibodies at a glence by displaying key datas, such as species reactivity, tested applications, epitope...

If the antibody your are looking for is not available on the market, keep in mind our custom services : Custom Rabbit and Chicken polyclonals, as well as RABBIT MONOCLONALS or GENE IMMUNIZATION (see our “Custom Services” chapter).

Thanks to : » its hudge database» its excellent technical support» and its ability to provide all associated products (secondary, substate, kits…)

INTERCHIM is your reliable supplier for primary antibodies.

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Hotline 04 70 03 73 06interbiotech@ interchim.comPrim'Ab specific topics

Suppliers

Interchim Prim’Ab suppliers are famous labs around the world, each of them with their own speciality.

■ Novus Biologicals» Wide range (almost 15000 with hundreds new each month)» Very well referenced in litterature» More and more sample sizes available» HIF (Hypoxia Inducible Factor) specialist

■ LifeSpan Biosciences» The IHC specialist » Wide range (over 35000 items with hundreds new each month)

LifeSpan,the IHC specialistSee our "Custom Services" chapter (p. xx) for more details.

■ Abnova» One of the largest antibody manufacturer in the world (over 20 000 available with hundreds new each month)» Over 50% from the catalogue is unique» Mouse polyclonals, monoclonals, and MaxPab®

MaxPab® antibodies :a new collection of polyclonal antibodies generated from annotated, sequence verified full-length proteins.• Power, precision, performance• Silencing efficiency assesment (most RNAi are available)• Both conformational and linear epitopes are recognized

■ Abgent» Sample sizes available for each catalog item» Core range focus on post-translationnal modification» Unique range in Autophagy area» Great antibody sampler kits for each research topic

Antibody sampler kitsSampler kits offer an affordable way to purchase multiple validated antibodies.

■ Spring Bioscience» Sample sizes available for each catalog item» Rabbit monoclonal antibodies (RabMabs)» Wide range (mainly focus on IHC)

Rabbit Monoclonal antibodies (RabMabs)RabMabs combine the high affinity of rabbit antibodies and very high monoclonal specificity.

■ Fitzgerald Industries International and Research Diagnostics Inc (RDI now parts of Fitzgerald)

» Wide range with a large part dedicated to Infectious diseases» Antigens

■ Meridian LifeScience (formerly Biodesign International) » Autoimmunity / Infectious diseases» Antigens

Meridian LifeScience now merges 4 brands :• Biodesign International• OEM concept• Viral antigens• Meridian Biologics

■ Genway Biotech » Chicken IgY specialist

IgY Chicken antibodiesIgY antibodies have multiple advantages over IgG antibodies, including higher yields, lower backgrounds, higher specificity, stronger avidity, and applicability to many immmunoassays.

■ And many othersInterchim works with over 50 antibodies suppliers, including :

See next pages for more details.





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Interesting topics and unique technical features

■ MaxPab® antibodies (over 9000 MaxPab® antibodies available and hundreds new each month)MaxPab® is a new collection of polyclonal antibodies generated from annotated, sequence verified full-length proteins. Each MaxPab® antibody is validated with a full-length human protein expressed from a mammalian transfected lysate, demonstrating high reactivity, sensitivity and specificity.

» Power, precision, performance» Silencing efficiency assesment (most RNAi are available)» Both conformational and linear epitopes are recognized» More epitopes recognized = chance to cross-react with other species» Broad application range

Applications WB siRNA knock-down

GNA15 MaxPab® polyclonal antibody 1 : GNA15 transfected 293T cell line lysate (41 KDa) 2 : Non-transfected 293T cell line lysate

SLC25A5 MaxPab® polyclonal antibodySLC25A5 expression in human Liver

CD14 MaxPab® polyclonal antibodyLane 1 : pCD-CD14 transfected HEK 293T cell lysate Lane 2 : pCD-CD14 and CD14 Pre-design Chimera RNAi co-transfected HEK 293T cell lysate

ELISA detection pair IHC IF

S100A7 MaxPab® polyclonal antibody• S100A7 capture Mab • Purified S100A7 recombinant protein• Biotinylated detection MaxPab®/streptavidin-HRP

ORC6L MaxPab® polyclonal antibodyImmunoperoxidase of purified MaxPab® antibody to ORC6L on formalin- fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml]

ING2 MaxPab® polyclonal antibodyImmunofluorescence of MaxPab® antibody to ING2 on HeLa cell .

■ Antibody sampler kitsSampler kits offer an affordable way to purchase multiple validated antibodies from Abgent's comprehensive antibody product lines. Assemble a collection of antibodies to your pathway of interest, or economically screen for the antibody that is perfect for your application.

Name P/N : QtyAcetylation Sampler Kit DS8960 5 antibodies or more (20ug each) to choose among the acetylation listApoptosis Sampler Kit KN1370 5 antibodies or more (20ug each) to choose among the apoptosis listAutophagy Sampler Kit KN1380 5 antibodies or more (20ug each) to choose among the autophagy listMethylation Sampler Kit DS8970 5 antibodies or more (20ug each) to choose among the methylation listNeurosciences Sampler Kit DS8980 5 antibodies or more (20ug each) to choose among the neurosciences listPhosphospecific Sampler Kit DS8910 5 antibodies or more (20ug each) to choose among the phosphospecific listStem Cell Sampler Kit DS8990 5 antibodies or more (20ug each) to choose among the stem cell listSumoylation Sampler Kit DS9000 5 antibodies or more (20ug each) to choose among the sumoylation listUbiquitination Sampler Kit DS9020 5 antibodies or more (20ug each) to choose among the ubiquitination list

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■ Rabbit monoclonal antibodies (RabMabs)RabMAbs combine the high affinity of rabbit antibodies and very high monoclonal specificity, previously only seen with mouse antibodies.These combined advantages account for the superior performance of RabMAbs in IHC.Technically RabMAbs provide superior performance :

» Affinity and specificity produce superior quality staining. (These can be markedly demonstrated with clone SP1 for Estrogen Receptor). Low expressing tissues in particular show superb clarity of staining.» High sensitivity ensures perfect staining and economical use of antibody at higher working dilutions.» The superior diagnostic capability of RabMAbs can be demonstrated using clone SP4 for Cyclin D1.» RabMAbs are ideally suited and greatly simplify double staining methods in IHC.» For research purposes RabMAbs eliminate most of the problems associated using mouse antibodies on mouse tissues.

Why Rabbit Monoclonals ?Rabbits are known to have more complex immune system than mice. They produce a high affinity polyclonal response to challenge by immunogens. The complexity of clones produced and their high affinity enables selection of the most appropriate antibody, combining high sensitivity and specificity.Advanced cloning methods have lead to the successful fusion of rabbit-B lymphocytes and a rabbit plasmacytoma cell line (240E) to produce very stable rabbit-rabbit hybridoma to guarantee continuity and quality of RabMAb supply.

References :1. Rossi S. et al "Rabbit Monoclonal Antibodies. A comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies." Am J Clin Pathol 2005 Aug 124(2):292-3022. Cano G., et al." Estimation of hormone receptor status in fine needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2", Diagn Cytopathol,2003 Oct ; 29(4):207-113. Cheuk W., et al "Consistent Immunostaining for Cyclin D1 can be achieved on a routin basis using newly available Rabbit monoclonal Antibody", .Am J Surg Pathol 2004 June 28(6):801-07

Selected list of rabbit monoclonal antibodies (full list includes about 700 antibodies and is available on our website : Prim’Ab search).

cerbB2

CD8

Cox2

CD79a

Antibody Name Clone P/N : Antibody Name Clone P/N :14-3-3 B/a E58 CQ7140 Chromogranin A SP12 MJ27504E-BP1 Y329 MG5480 COX2 SP21 MJ2912ALK / p80 SP8 MJ2692 Cyclin D1 SP4 MJ2590Aurora-A EP1008Y MH5220 Cytokeratin 20 SP33 MJ3052Bax E63 MH6920 Cytokeratin 5 SP27 MJ2962Bcl-2 E17 MG8020 DOG-1 SP31 MJ3012Bcl-x E18 MD5740 EGFR SP9 MJ2712beta-Tubulin EP1331Y MF6100 Estrogen Receptor SP1 MJ2511Calcitonin SP17 MJ2832 IL-4 EPR1118Y OA8770Calretinin SP13 MJ2770 Ki-67 SP6 MJ2640CaMKII EP1829Y MG7270 MIP1 alpha EP493Y OA8570Caspase 5 EP876Y MG4090 p53 SP5 MJ2620CD117 / c-kit SP26 MJ2952 Pax-5 SP34 MJ3072CD20 SP32 MJ3032 PLAP SP15 MJ2792CD23 SP23 MJ2932 Progesterone Receptor SP2 MJ2541CD3 SP7 MJ2672 PSMA SP29 MJ2992CD5 SP19 MJ2872 Synaptophysin SP11 MJ2730CD79a SP18 MJ2852 Tau, phospho E178 MG4410CD8 SP16 MJ2810 Ubiquitin EP296Y MH1740c-erbB-2 / HER-2 SP3 MJ2571 Vimentin SP20 MJ2890


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■ Chicken IgY antibodiesIgY antibodies have multiple advantages over IgG antibodies, including higher yields, lower backgrounds, higher specificity, stronger avidity, and applicability to many immunoassays.

Despite the overall similarities between IgY and conventionally used IgG antibodies, there are some profound differences in their chemical structures and biological features. IgY has a larger Fc region and a shorter hinge region than IgG. The IgY Fc region also has an additional pair of carbohydrate chains with unique sugar residues, important for highly efficient covalent coupling to solid surfaces such as microbeads or microarrays.

The affinity maturation process of IgY is via gene conversion, different from that of IgG via somatic mutation.

The phylogenetic distance between birds and mammals makes the chicken immune system often far more responsive to mammalian protein antigens thus generating highly reactive antibodies, particularly targeted to conserved proteins against which IgGs are difficult or impossible to raise.

The IgY production process is non-invasive and accumulative from collecting eggs, in contrast to that of IgG which requires bleeding and collecting sera from immunized animals.

The productivity of IgY is also superior, since one egg can generate about 100 mg of total IgY, about the yield of 50-100 mice.

» Polyclonal IgY antibodies have been shown to be suitable for all the conventional immunoassays such as Western blot, immunoprecipitation, immunohistochemistry, cell sorting, cell/tissue staining, etc. The quality of these assays using IgY antibodies is either comparable to or better than using conventional IgG antibodies.» IgY antibodies have also been shown to be very useful for cell or tissue immuno-staining (Immunohistochemistry and Immunocytochemistry). Both types of applications can implement high-throughput screening via tissue or cell array methodology. IgY antibodies are particularly more suitable for array (chip), nano-bar, or microbead applications, due to their higher stability, lower cross-reactivity, stronger binding (avidity), and greater detection sensitivity than those of IgG antibodies against the same antigens. Each of these features has been confirmed through side-by-side comparisons of the two types of antibodies under identical conditions.

A large selection of our antibodies and antigens are listed in chapter E. This table (by alphanumerical order of specificities) displays key information : species cross reactivity, host and quantity, compatible immunotechniques.

Advantages of IgY over IgGComparison IgY IgGAnimal Chicken of Avian mammalSource Egg Yolk PlasmaAvidity Hight ModerateQuantity Large LimitedCross Reactivity Low HightIsolation Simple ExpensiveStability Good GoodProductivity High/Fast Low/Slox

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Anti-Labels/ Loading control/ Phosphospecific-abs

■ Anti-Labels AntibodiesGoat Pab anti-HRP Rabbit Pab anti-HRP Mouse Mab anti-Biotin Mouse Mab anti-Digoxin Mouse Mab anti-FITCSize P/N : Size P/N : Size P/N : Size P/N : Size P/N :

Unconjugated 2 mg 735030 2 mg 447831 1 mg 872751 1 mg 314560 1 mg BI3470Biotin-SP 2 ml 301120 1.5 ml 755790 - - 0.5 ml 820630 0.5 ml FT0670HRP - - - - 0.5 ml M33331 0.5 ml M33340 0.5 ml FT0660Alk. Phos. 1 ml M33070 1 ml M43140 0.5 ml MC1060 0.5 ml M33350 0.5 ml FT0850AMCA 2 mg M33090 1.5 mg M43170 0.5 mg MC1090 0.5 mg M33370 0.5 mg FT0680Cy2TM 2 mg M33130 1.5 mg M43200 0.5 mg MC1120 0.5 mg M33390 0.5 mg FT0930FITC 2 mg 070390 1.5 mg M43160 0.5 mg MC1080 0.5 mg 150760 - -Cy3TM 2 mg 371080 1.5 mg M43180 0.5 mg MC1100 0.5 mg 266120 0.5 mg BH6800TRITC 2 mg 315720 1.5 mg M43130 0.5 mg MC1050 0.5 mg M33320 0.5 mg FT0420RRX 2 mg M33140 1.5 mg M43210 0.5 mg MC1130 0.5 mg M33400 0.5 mg FT0430Texas Red 2 mg M33080 1.5 mg M43150 0.5 mg MC1070 0.5 mg 319280 0.5 mg FT0750Cy5TM 2 mg M33100 1.5 mg M43190 0.5 mg MC1110 0.5 mg 281170 0.5 mg FT0920

■ Loading Control AntibodiesTarget Host Species cross-reactivities Tested applications Size P/N :Actin, cytoskeletal Rabbit Hu, Ms WB, IP 0.1 ml BY4270Actin, cytoskeletal Rabbit Hu, Ms WB, IP 0.1 ml BY4272Actin, cytoskeletal SuperNovus pack Rabbit Hu, Ms WB, IP 0.1 ml KU4770G6PD Rabbit Hu, Ms WB, IP 0.1 ml AW6700G6PD, agarose immobilized Rabbit Hu, Ms IP 0.1 ml GP8250GAPDH Rabbit Hu, Ms WB 0.1 ml KY4162GAPDH Rabbit Hu, Ms WB 0.1 ml KY4163GAPDH Rabbit Hu, Ms WB 0.1 ml KY4161GAPDH Rabbit Hu WB 0.1 ml KY4164GAPDH Rabbit Ms WB 0.1 ml KY4160Histone H4 Rabbit Hu WB, IP 0.1 ml KW0560Histone H4 Rabbit Hu WB, IP 0.1 ml KW0561Tubulin beta Rabbit Hu, (Ms, Rt, Ck, Pg...) WB 0.1 ml KZ4690

Non-exhaustive list. See Prim’Ab table in chapter E or the Prim’Ab search engine at www.interchim.com for more antibodies.

■ Antibodies against phosphorylated residues

Name P/N : Host Clone Isotype Size ApplicationpanPhospho Ser/Thr/Tyr (Pan) FM4590 Mouse SPM101 IgG1 100 µg WBphosphoserinePhosphoserine 978790 Rabbit polyclonal IgG 50 µg WB, IP, ELPhosphoserine - Biotin CF8930 Rabbit polyclonal IgG 50 µg WB, IP, ELPhosphoserine AA0620 Mouse BDI270 IgG1,k 100 µg WB, IP other clones availablePhosphoserine (Set of 6 MAbs) AL7330 Mouse set - 6x25 µg WB, IP, ELphosphothreoninePhosphothreonine CF5001 Rabbit polyclonal IgG 50 µg WB, IP, ELPhosphothreonine - Biotin CF8940 Rabbit polyclonal IgG 50 µg WB, IP, ELPhosphothreonine AA0570 Mouse BDI101 IgG1,k 100 µg WB, IP, EL other clones availablePhosphothreonine (Set of 3 MAbs) AL7310 Mouse set - 3x25 µg WB, IP, ELPhospho threonine-Proline CF8950 Rabbit polyclonal IgG 50 µg EL, WBPhospho threonine-any-phosphotyrosine CF8960 Rabbit polyclonal IgG 50 µg EL, WBphosphotyrosinePhosphotyrosine CF8970 Rabbit polyclonal IgG 50 µg EL, WBPhosphotyrosine - Biotin AL8760 Mouse BDI820 IgG1,k 100 µg WB, IP, ELPhosphotyrosine LH5420 Mouse PY20 IgG2b 100 µg IF, IP, WB, IHC other sizes availablePhosphotyrosine - FITC O13990 Mouse PY20 IgG2b 250 µg WB, IFPhosphotyrosine + Positive Control IS4660 Mouse BDI512 IgG1,k 100 µg WB, IP, EL other clones availablePhosphotyrosine (Set of 5 MAbs) AL7320 Mouse set - 5x25 µg WB, IP, EL


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Name P/N :14-3-3 protein (pS58) KM4210 BP14-3-3 protein (pT232) KM4220 BP14-3-3 zeta protein (pS58) MB9780 NP4E-BP1 (pT36) MC0020 NP4E-BP1 (pT45) MC0030 NP53BP1 (pS25) KQ5260 BPABL (pS588) KM4270 BPABL1 (pY134) KM4280 BPABL1 (pY245) KM4290 BPABL1 (pY251) KM4300 BPABL1 (pY272) KM4310 BPABL1 (pY276) KM4320 BPADD1 (pS726) MB9790 NPAFX (pS197) MB9450 NPAKT (pS473) MB8740 NPAKT (pS473) - Rabbit Mab JW2580AKT (pT308) MB8750 NPAKT (pY326) KM4360 BPAKT1 (pS124) KM4340 BPAKT1 (pS129) KM4350 BPAKT1 (pS472) (rat) T95950AKT1 (pS473 ) BY4510AKT1 (pT308) KP6930AKT1 (pY326) BY9170 BPAKT2 (pS474) MB9330 NPAMPK1 (pT174) MB9800 NPAMPK1/AMPK2 (pS485/S491) MB9720 NPAPBB1 (pS175) KM4370 BPAPBB1 (pS347) KM4380 BPApc1 (pS377) JW2980Apc6 (pT580) JW2990APP (pS198) BY9180 BPAPP (pS730) KM4390 BPAPP (pT668) JW2970APP (pY756) KM4400 BPAPP (phospho-668) MB9840 NPArrestin beta 1 (pS412) JW3000ASK1 (pS83) IS6720ASK1 (pS83) MB9760 NPASK1 (pS966) MB9770 NPATF2 (pS112 or 94) MB8550 NPATF2 (pS44) KP6970ATF2 (pS62 or 44) MB8510 NPATF2 (pS94) KP6980ATF2 (pT51) KP6990ATF2 (pT53) KP7000ATF2 (pT55) KP7010ATF2 (pT69 or 51) MB8520 NPATF2 (pT69/71) JW3010ATF2 (pT71 or 53) MB8530 NPATF2 (pT71) JW3020ATF2 (pT73 or 55) MB8540 NP

Name P/N :ATF4 (pS245) KP6960ATF4 (pS245) MB8730 NPATM (pS1981) MB9310 NPATM (pS1981) - Mouse Mab JW2590Aurora A (pT288) CF9770 BPAurora-A (pT288) for IHC CF1191BACE (pS498) KM4410 BPBad (mouse pS112) CF9780 BPBad (mouse pS136) CF9790 BPBad (mouse pS155) CF9800 BPBad (mouse pS155) - Mouse Mab GK2310 BPBad (pS112) MB8870 NPBad (pS118) BY9190 BPBad (pS128) JW3070Bad (pS134) KM4420 BPBad (pS136) JW3060Bad (pS155) MB8880 NPBad (pS75) KM4430 BPBad (pS99) KM4440 BPBCL-2 (pS70) MB8850 NPBCL-2 (pT56) MB8840 NPBcl-xL (pS62) JW2731BCL-XL (pS62) MB8860 NPBcr (pY177) MB9890 NPBid (mouse pS61) KM4450 BPBid (pS61) HP6590 BPBid (pS65) KM4460 BPBid (pS78) HP6430 BPBik (pT33) KM4470 BPBIM (pS69/65) MC0480 NPBLNK (pY84) JW3080BRCA1 (pS1189) AK9870 BPBRCA1 (pS1280) AK9880 BPBRCA1 (pS1387) AK9900 BPBRCA1 (pS1423) AK9910 BPBRCA1 (pS1457) AK9920 BPBRCA1 (pS1466) AK9940 BPBRCA1 (pS1524) MB9280 NPBRCA1 (pS1524) AK9860 BPBRCA1(pS1423) MC0170 NPc-Abl (pY245) JW3090c-Abl (pY412) MB9340 NPCaMKII (pT286) AW7660CaMKII (pT286) MC0470 NPCaMKII (pT305) AW9850CaMKII alpha (pT286) JW3110CaMKII alpha (pT286) - Mouse Mab JW2600Casein Kinase 2 beta (pS209) JW3120Caspase 6 (pS257) KM4480 BPCaspase 9 (pS196) KM4490 BPCatenin-alpha (pS641) MC0690 NPCatenin-beta (pS33) MB9980 NP

Name P/N :Catenin-beta (pS33) - Mouse Mab JW2570Catenin-beta (pS33/37) JW3130Catenin-beta (pS37) MB9990 NPCatenin-beta (pT41/S45) MB9270 NPCatenin-beta (pY654) - Mouse Mab IS7300Catenin-beta (pY86) - Mouse Mab IS7230Caveolin-1 (pY14) MB9090 NPCCNB1 (pS126) KM4710 BPCDC2 (pT161) KM4590 BPCDC2 (pT161) MB9430 NPCDC2 (pY15) MC0180 NPCDC25A (pS123) KM4500 BPCDC25A (pS177) KM4510 BPCDC25A (pS278) KM4520 BPCDC25A (pS292) KM4530 BPCDC25A (pS75) KM4540 BPCDC25A (pS75) MB9460 NPCDC25A (pT506) KM4550 BPCDC25B (pS186) KM4560 BPCDC25B (pS187) KM4570 BPCDC25B (pS353) KM4580 BPCDC25C (pS216) MB9290 NPCDC27 (pT244) JW3140CDC6 (pS54) KM4600 BPCDK1 (pS39) KM4610 BPCDK2 (pT160) KM4620 BPCDK2 (pT160) KM4630 BPCDK2 (pT160) MB9420 NPCDK7 (pT170) KM4640 BPc-Fos (pT232) JW3150c-Fos (pT325) JW3160Chk1 (pS280) KM4650 BPChk1 (pS317) KM4660 BPChk1 (pS345) MB9300 NPChk2 (pS516) MB9530 NPChk2 (pT68) MB8810 NPc-Jun (pS243) MB8470 NPc-Jun (pS63) KM4670 BPc-Jun (pS63) KM4680 BPc-Jun (pS63) MB8240 NPc-Jun (pS73) CF9810 BPc-Jun (pS73) KM4690 BPc-Jun (pS73) MB8260 NPc-Jun (pT239) MB8460 NPc-Jun (pT91) MB8430 NPc-Jun (pT91) - Mouse Mab JW2610c-Jun (pT93) MB8440 NPc-Jun (pY170) MB8450 NPc-Kit (pY568/Y570) JW3180c-Kit (pY703) JW3190c-Kit (pY721) MC0160 NPc-Kit (pY730) JW3220

Caption :NP : means Non-Phosphospecific match-paired antibody is available ; BP : means Blocking Peptide is available

Prim'Ab specific topicsPhosphospecific antibodies

■ Antibodies against phosphorylated proteinsThese antibodies are all rabbit polyclonals except when other host is specified.

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Name P/N :c-Kit (pY823) JW3230c-Kit (pY936) JW3210c-MET, see METc-Myc (pS373) KP7660c-Myc (pS58) KP7680c-Myc (pS62) - Mouse Mab JW2620c-Myc (pT358) KP7670c-Myc (pT58/S62) JW3240cofilin (pS3) MB9470 NPConnexin 43 (pS367) MC0290 NPConnexin 43 (pS368) JW3260Cortactin (pY421) JW3270Cortactin (pY466) MC0380 NPcPLA2 (pS505) CF9820 BPc-Raf (pS259) JW3290c-Raf (pS43) JW3320c-Raf (pS621) JW3310c-Raf (pY340/341) JW3330CREB (pS121) GP7660CREB (pS129) MC0390 NPCREB (pS129/133) JW3340CREB (pS133) KM4700 BPCREB (pS133) MB8720 NPc-Rel (pS503) KP7280c-Ret (pS696) JW3350Cyclin B1 (pS126) IS6400Cyclin E1 (pT395) KM4720 BPDab1 (pS491) JW3360Dab1 (pY198) JW3370Dab1 (pY220) JW3380DARPP32 (pT34) GP7530DARPP32 (pT75) GP7390DAXX (pS213) KM4730 BPDAXX (pS495) KM4740 BPDAXX (pS517) KM4750 BPDNA PKcs (pT2609) MB9690 NPDNA PKcs (pT2609) - Mouse Mab JW2630DOK2 (pY142) JW3410Dynamin (pS774) AW9830Dynamin (pS778) AW9840E2A (pT355) GR5980 BPE2F1 (pS337) KM4760 BPEGFR (pS1047) - Mouse Mab IS7410EGFR (pS1070) MB8990 NPEGFR (pT654) - Mouse Mab IS7280EGFR (pT678) MB9820 NPEGFR (pT693) MB9830 NPEGFR (pY1016) JW3480EGFR (pY1045) - Mouse Mab IS7180EGFR (pY1068) JW3420EGFR (pY1086) JW3430EGFR (pY1092) MB9000 NPEGFR (pY1110) MC0330 NPEGFR (pY1148) JW3440EGFR (pY1172) MC0000 NPEGFR (pY1173) JW3450EGFR (pY1197) MC0070 NP

Name P/N :EGFR (pY845) JW3460EGFR (pY845) - Mouse Mab IS7640EGFR (pY869) MC0080 NPEGFR (pY992) JW3470eIF2A (pS51) MC0430 NPeIF2A (pS52) JW3490eIF2B epsilon (pS539) JW3500eIF4E (pS209) MC0120 NPeIF4G (pS1108) JW3520Elk (pS383) JW3530Elk1 (pS383) BC4150Elk1 (pS383) MB8270 NPElk1 (pS389) MB8590 NPElk1 (pT417) MB8600 NPeNOS (pS116) JW3540eNOS (pS1177) - Mouse Mab AL8590EphrinB (pY298) JW3550EphrinB (pY317) JW3560EphrinB (pY331) JW3570ErbB2 (pY1112) KM4770 BPErbB2 (pY1248) JW3590ErbB2 (pY1248) KM4780 BPErbB2 (pY871) KM4790 BPERK1/2 (pT185/T202) (FITC) JW3610ERK1/2 (pT202/183 + Y204/185) JW3620ERK3 (pS189) KM4800 BPERK5 (pT218) JW3630ESPL1 (pS1176) KM4810 BPESPL1 (pS748) KM4820 BPESPL1 (pS801) KM4830 BPEstrogen Recepteur alpha (pS305) KQ8190 BPEstrogen Receptor alpha (pS104) MB8890 NPEstrogen Receptor alpha (pS104/106)

CF9830 BP

Estrogen Receptor alpha (pS106) MB8900 NPEstrogen Receptor alpha (pS118) MB8910 NPEstrogen Receptor alpha (pS167) MB8920 NPETS1 (pS251) JW3660ETS1 (pS282) JW3670ETS1 (pS282/285) JW3680ETS1 (pT38) JW3690EZH2 (pS21) HP6690 BPEzrin (pT566) MB9910 NPEzrin (pY353) MB8830 NPFADD (mouse pS191) KM4840 BPFAK (pS722) JW3700FAK (pS732) JW3770FAK (pS843) JW3780FAK (pS910) JW3710FAK (pY397) JW3720FAK (pY397) - Rabbit Mab JW2640FAK (pY407) JW3730FAK (pY576) JW3740FAK (pY577) JW3750FAK (pY861) MB8790 NPFAK (pY925) MB9320 NPFanconi anemia D2 (pS222) JW3790

Name P/N :FGFR (pY653/654) CE7570 BPFGFR (pY766) KM4850 BPFKHR (pS256) MB9260 NPFKHR (pS319) MB9440 NPFKHRL1 (pS253) MB9600 NPG3BP-1 (pS232) MB9010 NPGab1 (pY627) KM4880 BPGab1 (pY627) MC0500 NPGAP43 (pS41) AW7700GATA1 (pS142) MB8620 NPGATA1 (pS310) MB8630 NPGATA4 (pS105) JW3810GluR1 (pS849) MC0310 NPGlutamate receptor 2 (Prec.)(pS880) MC0510 NPGlycogen Synthase (pS640) JW3820Glycogen Synthase (pS641/645) JW3890GRK2 (pS670) JW3840GSK3 alpha (pS21) MB8300 NPGSK3 alpha + beta (pY216/Y279) JW3850GSK3 beta (pS21/29) KM4900 BPGSK3 beta (pS9) KM4910 BPGSK3 beta (pS9) MB8250 NPGSK3 beta (pY216) MC0560 NPH2AX gamma (pS139) AZ3810 BPHck (pY207/S211) JW3900HDAC4 (pS632) MB9850 NPHDAC5 (pS498) MB9860 NPHDAC8 (pS39) MB9370 NPHdmX / MDM (pS403) HP6910 BPHER2 (pY1112) KM4930 BPHER2 (pY1221) KP7140HER2 (pY1221/Y1222) MB8950 NPHER2 (pY1222) KP7160HER2 (pY1248) MB8980 NPHER2 (pY1248) - Mouse Mab N67020HER2 (pY877) MB8940 NPHER-2 (phospho) - Mouse Mab N67020HER4 (pY1162) KM4940 BPHER4 (pY1188) KM4950 BPHGFR (pY1230) CF9840 BPHGFR (pY1234/5) CF9850 BPHIST1H3B3 (pS10) KM4920 BPHistone H2A.X (pS139) MC0360 NPHistone H3 (pS10) KM4230 BPHistone H3 (pS10) KM4240 BPHistone H3 (pS10) - Mouse Mab GK2400 BPHistone H3 (pS10) - Mouse Mab GK2410 BPHistone H3 (pS10) - Mouse Mab GK2420 BPHistone H3 (pS28) KM4250 BPHistone H3 (pS28) KM4260 BPHistone H3 (pS28) - Rat Mab JW2680Histone H3.1 (pS10) MB9810 NPHSF1 (pS303) MC0320 NPHSP25 (pS86) JW3910HSP27 (pS15) MB9670 NPHSP27 (pS78) MC0210 NPHSP27 (pS82) MC0220 NP

Légende :NP : means Non-Phosphospecific match-paired antibody is available ; BP : means Blocking Peptide is available



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Name P/N :HSP90B (pS254) MB9870 NPICAM-1 (pY512) MB9020 NPIFNAR1 (phopho S535/539) JW3930IGF-1R (pY1161) MB9060 NPIGF-1R (pY1165/Y1166) MB9070 NPIGF1R, see Insulin ReceptorIKB alpha (pS32/36) JW3940IKB alpha (pS32/S36) MB9570 NPIKB alpha (pY42) MB9650 NPIKB beta (pS23) MC0570 NPIKB epsilon (pS22) MB9940 NPIKK alpha (pS176/S180) JW3950IKK alpha (pT23) MB9380 NPIKKb (pY199) KM4960 BPIKKg (mouse pS375) KM4980 BPIKKg (pS31) KM4970 BPIKKg (pS43) KM4990 BPIL1R (pY496) KM5000 BPIL3R (pY593) KM5010 BPIL3R (pY766) BY9200 BPINSR (pY1361) KM5020 BPInsulin Receptor (pY1158) JW3970Insulin Receptor (pY1158/1162/1163) JW3980Insulin Receptor (pY1161) KP7490Insulin Receptor (pY1162/1163) JW3990Insulin Receptor (pY972) JW3960Integrin alpha 4 (pS988) JW4000Integrin beta 1 (pS785) JW4020Integrin beta 1 (pT788/T789) JW4010Integrin beta 3 (pY773) MB8800 NPIntegrin beta 3 (pY785) MC0450 NPIntegrin beta 4 (pY1422) CF9870 BPIntegrin beta 4 (pY1440) CF9880 BPIntegrin beta 4 (pY1526) CF9890 BPIntegrin beta 4 (pY1642) CF9900 BPIRAK1 (pS376) BY9210 BPIRE1 alpha (pS724) JW4050IRS1 (pS307) MC0140 NPIRS1 (pS312) MB9490 NPIRS1 (pS616) JW4060IRS1 (pS636) MC0100 NPIRS1 (pS639) MC0090 NPIRS1 (pY1179) JW4070IRS1 (pY1229) JW4080IRS1 (pY612) JW4110IRS1 (pY896) JW4090IRS1 (pY941) JW4120IRS2 (pS731) JW4130JAK1 (pY1022) MB9540 NPJAK1 (pY1022/1023) JW4140JAK2 (pY1007) MB9560 NPJAK2 (pY1007/1008) JW4150JAK2 (pY221) MB9550 NPJNK1+JNK2 (pT183/Y185) JW4160JunB (pS259) MB8490 NPJunB (pS79) MB8480 NPJunD (pS255) MB8500 NP

Name P/N :KAP-1 (pS824) KQ4830 BPKDR (pY1175) CF9910 BPKDR/FLK1 (pY1214) KP7400KDR/FLK1 (pY951) KM4860 BPKDR/FLK1 (pY996) KM4870 BPKeratin 18 (pS33) MC0580 NPKeratin 18 (pS33) - Mouse Mab U99050Keratin 8 (pS431) - Mouse Mab U98860Keratin 8 (pS73) - Mouse Mab U98980LAT (pY132) CF9920 BPLAT (pY171) CF9930 BPLAT (pY191) JW4170Lck (pS158) JW4200Lck (pY192) JW4210Lck (pY393) MB9500 NPLck (pY505) JW4190LIMK 1/2 (pT507/Y508) JW4220LIMK1 (pT508) MB9350 NPLIMK2 (pT505) MB9360 NPLipoxygenase (pS523) JW4230LLGL1/2 (pS650/654) KM3770 BPLPR1 (pS452) KM5030 BPMAPKAPK1 (pS364) KM5040 BPMAPKAPK2 (pS272) KM5050 BPMAPKAPK2 (pT334) MC0590 NPMAPKAPK2 (pT334) JW4240MAPKAPK5 (pS212) KM5060 BPMAPKAPK5 (pS93) CF9950 BPMARCKS (pS158) MC0520 NPMBP (pT98) - Mouse Mab CF5220MCM2 (pS108) BD0700 BPMCM2 (pS108), for IHC KQ6640MCM2 (pS27) GR4350 BPMCM2 (pS27), for IHC KQ4570MCM2 (pS40/S41) KQ8070 BPMCM2 (pS40/S41), for IHC KQ6610MCM2 (pS41) GR5790 BPMCM2 (pS41), for IHC KQ3980MCM2 (pS53) KQ5080 BPMDM2 (pS185) JW4260MeCP2 (pS178) CF9960 BPMeCP2 (pS292) KM5070 BPMEF2A (pS408) MC0600 NPMEF2A (pT312) MB8610 NPMEK1 (pS217) MB9920 NPMEK1 (pS217/221) KM5080 BPMEK1 (pS221) MB9640 NPMEK1 (pS298) JW4270MEK1 (pT291) MC0530 NPMEK1 (pT292) BC4140MEK1 (pT386) BC4130MEK1/2 (pS218/222) AW7730MEK1/2 (pS218/222) CF9970 BPMEK1/2 (pS222) JW4300MEK2 (pT394) MB8310 NPMEK3 (pS189) KM5090 BPMEK3/6 (pS189) JW4330

Name P/N :MEK4 (pS257/T261) JW4340MEK4 (pT261) KM5100 BPMEK5 / MAPKK5 (pS311/T315) CF5050MEK6 (pS202) KM5110 BPMEK7 (pS271/T275) JW4350Merlin (pS518) MC0340 NPMERTK (pY749/753/754) JW4360MET (pY1230/1234/1235) JW4370MET (pY1365) JW4400MET (pY1003) KM5120 BPMET (pY1234) MC0060 NPMET (pY1349 ) KM5130 BPMET (pY1349) KM5140 BPMET (pY1349) KM5150 BPMET (pY1349) MC0150 NPMET (pY1356) KM5160 BPMET/HGFR (phospho) - Mouse Mab GK2200 BPMKK3 (pS189) KM5170 BPMKK3 (pS189) MB9510 NPMKK6 (pS207) MB9520 NPMLCK smooth muscle (pS1760) JW5380Mlf1 (phospho) GL8580 BPMLK3 (phosphoT277/S281) JW4410MSK (pS360) KM5180 BPmTOR (pS2448) MC0010 NPMunc-18 (pS515) JW4430Myc (pS373) MB8580 NPMyc (pT358) MB8570 NPMyc (pT58) MB8560 NPMyoD (pS200) MB8960 NPMyosin light chain (pS19) JW4450Myosin light chain (pS20) JW4440MYT1 (pT495) KM5190 BPNBS1 (human pS343) GR4700 BPNBS1 (mouse pS343) GR5780 BPNeurofibromatosis 2 gene prod. (pS518)

IS4430

Neurofilament (200kD phospho) - Ms Mab

Q34750

NF2 (pS518) JW4470NFAT1 (pS54) JW4480NFKB (pS536) KM5210 BPNFkB p100 (pS865) KP7760NFkB p105 (pS337) KP7790NFkB p105 (pS893) KP7800NFkB p105 (pS907) KP7810NFKB p65 (pS536) KM5200 BPNFKB-p100/p52 (pS865) MB8380 NPNFKB-p100/p52 (pS869) MB8390 NPNFKB-p105/p50 (pS337) MB8400 NPNFKB-p105/p50 (pS893) MB8410 NPNFKB-p105/p50 (pS907) MB8420 NPNFKB-p105/p50 (pS932) MC0240 NPNFKB-p105/p50 (phospho-Se927) MC0610 NPNFKB-p65 (pS276) MB8340 NPNFKB-p65 (pS311) MC0300 NPNFKB-p65 (pS468) MB8360 NPNFKB-p65 (pS529) MB9970 NP



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Name P/N :NFKB-p65 (pS536) MB8370 NPNFKB-p65 (pT254) MB8330 NPNFKB-p65 (pT435) MB8350 NPNMDA NR2A (mouse/rat phospho) GG4170NMDA NR2B (mouse/rat phospho) GG4180NMDA NR2B (pY1252) JW4500NMDA NR2B (pY1336) JW4510NMDA NR2B (pY1472) AW7630NMDAR1 (pS896) MB9170 NPn-Myc (pS54) BU2610Nudel (pS231) KM5220 BPNudel (pS242) KM5230 BPNudel (pT219) KM5240 BPNudel (pT245) KM5250 BPN-WASP (pY256) JW4460OBRb (mouse pY1138) D14800OBRb (mouse pY985) H24820p16-INK4A (pS140) KM5260 BPp16-INK4A (pS152) KM5270 BPp16-INK4A (pS7) KM5280 BPp16-INK4A (pS8) KM5290 BPP21CIP1 (pS146) KM5300 BPP21CIP1 (pT145) KM5310 BPP21CIP1 (pT57) KM5320 BPp27Kip1 (pS10) KM5330 BPp27Kip1 (pS178) KM5340 BPp27Kip1 (pT157) KM5350 BPp27Kip1 (pT187) KM5360 BPp27Kip1 (pT187) MB9930 NPp38 (pY182/T180) JW4520P38 MAPK (pT180) MC0250 NPP38 MAPK (pY182) MC0260 NPp38MAPK (pT180/Y182) AW7740p44/42 MAP Kinase (pY204) MC0200 NPp44/42 MAP Kinase (pT202) MC0190 NPp53 (pS15) MB9110 NPp53 (pS20) KM5370 BPp53 (pS315) KM5380 BPp53 (pS315) MB9140 NPP53 (pS33) KM5390 BPp53 (pS33) KP7920p53 (pS37) KM5400 BPp53 (pS376) KM5410 BPp53 (pS378) KM5420 BPp53 (pS392) AW7750p53 (pS392) - Mouse Mab V03020p53 (pS46) MB9130 NPp53 (pS6) JW4540p53 (pS9) JW4550p53 (pT18) KM5430 BPp56Dok-2 (pY299) MC0420 NPp62Dok(pY362) MC0400 NPp62Dok(pY398) MC0410 NPp70 S6 Kinase (pS424) MC0460 NPp70 S6 Kinase (pT421) MC0270 NPp70 S6 Kinase (pS411) MC0370 NPp73 (pY99) MB8780 NP

Name P/N :p90RSK(pT348) MB9180 NPp95/NBS1 (pS343) MB8770 NPPAG3 (pY724) JW4570PAG3 (pY763) JW4580PAK 1 (pS199/204) CF9980 BPPAK 1 (pT212) MB9590 NPPAK 1/2/3 (pS141) JW4620PAK 1/2/3 (pT402) JW4590PAK 1/2/3 (pT423) JW4600PAK 1/2/3 (pT423/T402/T421) MB9680 NPPaxillin (pS126) JW4650Paxillin (pS178) GR5490 BPPaxillin (pY118) MB9080 NPPaxillin (pY31) MB9900 NPPDE4A (pS) JW4670PDGF Receptor alpha (pY742) JW4690PDGF Receptor alpha (pY754) JW4680PDGF Receptor alpha (pY762) JW4700PDGFR (pY740) KM5450 BPPDGFR alpha + beta (pY572 + Y574) JW4710PDGRF (pY579) KM5440 BPPDK1 (pS241) KM5460 BPPDK1 (pS241) MB8280 NPPED / PEA 15 (pS116) JW4720PHF (pS396) - Mouse Mab IS7630PHF (pT231) - Mouse Mab IS7430phospholamban (pS16) CB7350phosphotyrosine (pY120) - Mouse Mab

JW2670

PKA a+b (catalytic subunits) (pT197) JW4780PKA alpha (pY197) CF9990 BPPKA beta (catalytic subunit) (pS338) JW4790PKA phosphopep. Substrate - Mouse Mab

AL8680

PKA2 beta (regul. subunit) (pS114) JW4770PKC alpha (pT638) JW4800PKC alpha (pY497) CG0000 BPPKC beta (pT642) MB9700 NPPKC beta 1 (pT642) JW4810PKC beta 1 + beta 2 (pT500) JW4820PKC beta 2 (pT641) JW4830PKC delta (pS645) JW4840PKC delta (pS645) MC0540 NPPKC delta (pS664) JW4850PKC delta (pY311) JW4860PKC epsilon (pS729) JW4870PKC eta (pS674) JW4880PKC eta (pT655) JW4890PKC gamma (pT514) JW4900PKC gamma (pT655) JW4910PKC gamma (pT674) JW4920PKC iota + lambda (pT555/563) JW4930PKC mu / PKD (pS738/742) JW4970PKC theta (pS345/348) CF5030PKC theta (pS676) JW4950PKC theta (pS676) MC0550 NPPKC theta (pS695) JW4960PKC theta (pS695) MB9710 NP

Name P/N :PKC theta (pT538) JW4940PKC zeta (pT410) MC0620 NPPKD/PKC mu (pS738) MB8970 NPPKD/PKC mu (pS910) MB9120 NPPKG phosphopep. Substrate - Mouse Mab

AL8630

PKR (pT446) MC0440 NPPKR (pT451) MC0490 NPPKR (pY258) CG0010 BPPKR (pY446) CG0020 BPPKR (pY451) CG0030 BPPKR (pY451) (mouse) CG0040 BPPLCg-1 (pY783) MB9160 NPPLCg-2 (pY753) MB9730 NPPLM (pS68) - Mouse Mab GK2370 BPPLM (pS83) (mouse) KM6030 BPPLM (pS88) (mouse) KM6020 BPPolo Like Kinase Kinase(pS482/486/490)

JW5000

Potassium Channel, Kv3.1 (pS503) BU9600PPAR alpha (pS12) (mouse) CF5200PPAR alpha (pS21) (mouse) CF5170PRAS 40 (pT246) JW5010Progesterone Receptor (pS190) MB8930 NPProgesterone Receptor (pS190) - Mouse Mab

AW5240

Progesterone Receptor (pS294) - Mouse Mab

AW5250

PTEN (pS370) MB8820 NPPTEN (pS380) KM5470 BPPTEN (pS380) MB8320 NPPTEN (pS380/T382/T383) MB8760 NPPTEN (pS380/T382/T383/S385) JW5040PTPa (pS180) KM5480 BPPTPa (pS204) KM5490 BPPYK2 (pY402) MB9960 NPPYK2 (pY579) JW5060PYK2 (pY579/Y580) JW5070PYK2 (pY580) JW5080PYK2 (pY881) JW5090Rabphilin 3A (pS234) JW5100RAC1 + Cdc42 (pS71) JW5110Rad17 (pS645) AL0620 BPRad9 (pS272) KM5500 BPRad9 (pS277) KM5510 BPRad9 (pS328) KM5520 BPRad9 (pS336) KM5530 BPRad9 (pS341) KM5540 BPRad9 (pS375) KM5550 BPRad9 (pS380) KM5560 BPRad9 (pS387) KM5570 BPRad9 (pY28) KM5580 BPRaf-1 (pS259) MB8290 NPRaf-1 (pS301) JW5130Raf-1 (pS642) JW5120Raf-1 (pY341/S339) - Rat Mab V17670Rb (pS249) KM5600 BPRb (pS608) KM5610 BP




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Name P/N :Rb (pS608) - Mouse Mab V09130RB (pS612) KM5620 BPRb (pS780) KM5630 BPRb (pS780) MB9410 NPRb (pS788) KM5640 BPRb (pS795) KM5650 BPRb (pS795) MB9390 NPRb (pS807) KM5660 BPRb (pS807) MB9400 NPRb (pS807/811) JW5160Rb (pS811) KM5670 BPRb (pT252) KM5680 BPRb (pT356) JW5170Rb (pT821) KM5690 BPRb (pT826) KM5700 BPRb-like-1 (pS975) KM5590 BPRelB (pS552) MC0280 NPRibosomal Protein S6 (pS236) JW5230RNA Polymerase II (pS2) KQ5000 BPRNA Polymerase II (pS5) KQ6970 BPRNA polymerase II CTD (pS5) - Mouse Mab

JW2690

ROCKII (pT396) KP8090RPA32 (pS33) GR3450 BPRPA32 (pS4/S8) BF4170 BPRPS6 (pS235) KQ3410 BPRPS6 (pS235/236) KQ7590 BPRPS6 (pS244/247) JW2840RPS6 pAbVantage Pack KQ7060RSK1 p90 (pS221) JW5260RSK1 p90 (pS363) JW5270RSK1 p90 (pS380) JW5280S6 Ribosomal Protein (pS235) MC0110 NPS6K (pT229) JW5290SAPK/JNK (pT183) MC0230 NPSEK1/MKK4 (pS80) MB9750 NPSEK1/MKK4 (pT261) MB9740 NPSEPARIN (pS1073) KM5720 BPSEPARIN (pS1126) KM5730 BPSEPARIN (pS1501) KM5710 BPSHC (pY239/240) JW5300Shc1 (pY349) MC0630 NPShc1 (pY427) MC0640 NPSHP2 (pS576) JW5310SHP2 (pY542) MC0650 NPSHP2 (pY580) MC0660 NPSmad1 (pS465) MC0670 NPSmad2 (pS465/467) JW5340Smad2 (pS467) MC0680 NPSmad2 (pT8) JW5350SMAD3 (pS204) KM5740 BPSMAD3 (pS208) KM5750 BPSMAD3 (pS213) KM5760 BPSmad3 (pS423/425) JW5360SMC1 (pS957) KM5770 BPSMC1 (pS957) MB9880 NPSMC1 (pS966) AL0330 BP

Name P/N :SMC1 (pS966), for IHC KQ6550SMC3 (pS1083) HP7550 BPSMC3 (pS383) AL0360 BPSOX9 (pS181) JW5390Src (pY215) KM5780 BPSrc (pY418) MB9100 NPSrc (pY529) MB9580 NPSrc Family Neg. Reg. pSite (pY522) JW5420STAT1 (pS727) CG0050 BPSTAT1 (pS727) KM5800 BPSTAT1 (pS727) - Mouse Mab JW2700STAT1 (pY701) KM5810 BPSTAT1 (pY701) MB8640 NPSTAT1(pS727) MB9660 NPSTAT3 (pS727) KM5820 BPSTAT3 (pS727) MB8660 NPSTAT3 (pY705) KM5830 BPSTAT3 (pY705) MB8650 NPSTAT3 (pY727) KM1700 BPSTAT4 (pS722) KM5840 BPSTAT4 (pY693) KM5850 BPSTAT4 (pY693) MB8670 NPSTAT5 (pY694) JW5480STAT5 (pY695/699) - Mouse Mab IS7650STAT5a (pS726) KM5860 BPSTAT5a (pS780) MB8690 NPSTAT5a (pY694) KM5870 BPSTAT5a (pY694) MB8680 NPSTAT6 (pT645) MB8710 NPSTAT6 (pY641) KM5880 BPSTAT6 (pY641) MB8700 NPSTAT6 (pY645) KP8230Stathmin 1 (pS15) MC0130 NPStathmin 1 (pS24) MC0040 NPStathmin 1 (pS37) MC0050 NPSurvivin (pT34) - Astrocyte Marker AW8690Syk (pY323 human / Y317 mouse) JW2900Syk (pY525/526) KM5890 BPSynapsin (pS549) JW5500Synapsin (pS62/67) JW5510Synapsin (pS9) MC0350 NPSynuclein alpha (pS129) JW5520TAO 2 (pS181) GP8000Tau (pS199) JW5590Tau (pS199/202) JW5530Tau (pS202) JW5600Tau (pS214) MB9220 NPTau (pS235) MB9190 NPTau (pS262) MB9240 NPTau (pS356) JW5620Tau (pS396) MB9150 NPTau (pS400) JW5630Tau (pS404) MB9250 NPTau (pS409) JW5640Tau (pS422) JW5650Tau (pT181) MB9200 NPTau (pT205) MB9210 NP

Name P/N :Tau (pT212) JW5670Tau (pT217) JW5680Tau (pT231) MB9230 NPTH (pS40) CG0060 BPTNFR (mouse pS299) KM5920 BPTNFR (pS274) KM5910 BPTNFR1 (mouse pS269) KM5900 BPTNIK (pS769) KM5930 BPTNIK (pS769) KM5940 BPTopo II alpha (pT1342) - Mouse Mab CF5130TrkA (pY490) KM5950 BPTrkA (pY674/675) KM5960 BPTrkA (pY676) KM5970 BPTrkA (pY791) KM5980 BPTroponin I-Cardiac (phospho) - Mouse Mab

O22290

Tryptophan Hydroxylase (pS260) JW5700TyK2 (pY1054/1055) KM5990 BPTyrosine Hydroxylase (pS19) 768444Tyrosine Hydroxylase (pS31) 768443Tyrosine Hydroxylase (pS40) AW7760VASP (pS157) MB9950 NPVASP (pS238) MB9610 NPVav (pY174) MB9480 NPVAV1 (pY160) JW5710VAV3 (pY173) JW5720VEGF Receptor 2 (pY1054) JW5750VEGF Receptor 2 (pY1054/1059) JW5730VEGF Receptor 2 (pY1214) JW5760VEGFReceptor 2 (pY1175) MB9030 NPVEGFReceptor 2 (pY1214) MB9040 NPVEGFReceptor 2 (pY951) MB9050 NPVimentin (pS55) - Mouse Mab JW2710Vinculin (pY100) JW2880Vinculin (pY1065) JW2890Vinculin (pY822) JW5770WASP (pS483/S484) BH9900 BPWee1 (pS123) KM6000 BPWee1 (pS53) KM6010 BPXRCC 1 (pS461) GR5590 BPXRCC 1 (pS475) GR3500XRCC 1 (pS485/T488) GR6060 BPXRCC 1 (pS518/T519/T523) GR4760 BPYES1 (pY527) KM5790 BPZap-70 (pY292) JW2850Zap-70 (pY315/319) JW2860Zap-70 (pY319) MB9620 NPZap-70 (pY493) MB9630 NP



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Epitope Tag Antibodies

Tag Host Tag sequenceP/N :

Unconj. HRP FITC Other*6-His Chicken HHHHHH X MD1790 KX2390 X6-His Mouse Mab HHHHHH GK2290 OA2180 OA2190 OA2170 (B)6-His Rabbit HHHHHH MD7681 X OG2950 XAU1 Goat DTYRYI KW3521 KX0160 KX0170 GP8180 (A)AU1 Rabbit DTYRYI KU6151 X X XAU5 Goat TDFYLK KU5902 KX0090 KY2270 GP8310 (A)AU5 Rabbit TDFYLK KU5311 X X XBTag Rabbit QYPALT CF5920 X X XC-myc Chicken AW4270 ME6630 MG1060C-myc Goat AW4520 AW4560 AW4550 AW4540 (A)

C-myc Mouse Mab AEEQKLISEEDLLRKRREQLKHKLE GK2260 X OA1800 X

C-myc Rabbit ME6930(monoclonal)

AW6490 (polyclonal)

DYKDDDDK Mouse Mab DYKDDDDK OB0000 OB0010 OB0020 (B)DYKDDDDK Rabbit DYKDDDDK CF5910 X X XECS Chicken DDDDK AW4290 X X XECS Goat DDDDK AW4570 AW4610 AW4600 AW4590 (A)ECS Rabbit DDDDK AW9660 AW6620 E-Tag Goat GAPVPYPDPLEPR KU6842 KZ3760 KZ1170 KU5230 (A)E-Tag Rabbit GAPVPYPDPLEPR KU6672 KU6671 X XGalactosidase beta Rabbit GS8760 GS8800 GS8790 GS8770 (A)GFP Mouse Mab GK2280 X X X

* A = Agarose Immobilized ; B = Biotin

Western blot analysis of anti-GFP Mab (Cat. # GK2280) using purified GFP, YFP and BFP proteins expressed in bacteria : Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab.

Immuno-fluorescence for GFP Tag Antibody (Cat # GK2280). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing.Data courtesy of Seth Robertson, Boston College.


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Epitope Tag Antibodies

HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody (Cat.# GK2270).

Western blot analysis of anti-HA Tag Antibody (Cat.# GK2270). in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.

Tag Host Tag sequenceP/N :

Unconj. HRP FITC Other*Glu-Glu Chicken EYMPME AW4310 X X XGlu-Glu Goat EYMPME AW4620 AW4660 AW4650 AW4640 (A)Glu-Glu Rabbit EYMPME AW9680 AW6920 X XGST Goat AW4670 AW4700 AW4710 AW4690 (A)

Mouse Mab GK2300GST Rabbit GK4380 AW6940 X XHA Chicken YPYDVPDYA AW4360 X X XHA Goat YPYDVPDYA AW4720 AW4760 AW4750 AW4740 (A)HA Mouse Mab YPYDVPDYA GK2270 X X XHA Rabbit YPYDVPDYA S42812 AW6950 X XHSV Goat SQPELAPEDPED KU6591 KZ3110 KY6380 KX1860 (A)HSV Rabbit SQPELAPEDPED KU7061 KZ0280 X XKT3 Goat KPPTPPPEPET KY4042 KY4041 KY4040 GP8410 (A)KT3 Rabbit KPPTPPPEPET KZ3210 X X XLuciferase Rabbit N91750 KU5200 KU5250 XMBP Mouse Mab GU8980 GU9010 X XProtein C Rabbit CEDQVDPRLIDGK CF5930 X X XS-Tag Goat KETAAAKFERQHMDS KU6622 KZ3810 KZ3590 KU5821 (A)S-Tag Rabbit KETAAAKFERQHMDS KU7622 KZ4290 X XT7 Chicken MASMTGGQQMG AW4370 X X XT7 Goat MASMTGGQQMG AW4810 AW4850 AW4840 AW4830 (A)T7 Rabbit MASMTGGQQMG AW9780 AW8250 X XV5 Chicken GKPIPNPLLGLDST AW4390 X X XV5 Goat GKPIPNPLLGLDST AW4860 AW4900 AW4890 AW4880 (A)V5 Rabbit GKPIPNPLLGLDST KM1760 AW8340 X XVSV-g Goat YTDIEMNRLGK KX5690 KW7700 KW4110 GP8160 (A)VSV-g Rabbit YTDIEMNRLGK AK9461 X X X

* A = Agarose Immobilized ; B = Biotin

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