FLOW CYTOMETRY

Flow cytometry is a technology that simultaneously measures, and then analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light, usually a laser.

DEFINITION

What can a flow cytometer tell us about a cell Its relative size (Forward Scatter—FSC)Its relative granularity or internal complexity (Side Scatter—SSC) Its relative fluorescence intensity (FL1, FL2, FL3, and FL4)

BASICS OF FLOW CYTOMETRY

Components of FLOW CYTOMETRY: