TECO ProjectTechnological Eco-Innovations for the Quality Control and the Decontamination of Polluted Waters and Soils

TECO Mobility grantBiochemical and molecular-based techniques to study

microbial diversity of the rhizosphere and its application to soils of anthropized environments

Tel: +39 06 9067 2741E-mail: teco.challenge@gmail.com

Website: www.indoitalianresearch.eu

Beneficiary of the grant: Dr. Ana Carolina AGNELLOHome Institution/Company (EU): LGE-UPEM (France)Host Institution/Company (INDIA): CSIR-NBRI (Lucknow, U.P.)Period of the stay in India: 14/09/16 - 05/12/16

TECO ProjectTechnological Eco-Innovations for the Quality Control and the Decontamination of Polluted Waters and Soils

Tel: +39 06 9067 2741E-mail: teco.challenge@gmail.com

Website: www.indoitalianresearch.eu

Objectives of the project

• To gain knowledge and training in biochemical and molecular-based techniques to study soil microbial diversity

• To analyze the diversity of microbial communities of the rhizosphere of anthropized soil samples§ by:a) Community Level Psysiological Profiling (CLPP) andb) Denaturing Gradient Gel Electrophoresis (DGGE)

§ Soil samples issue of a previous pot experiment with sunflower ( Helianthus annus ) growing in the presence of metal-rich (Cu, Pb and Zn) slags

Pot experiment with sunflowerSoil/metal-rich slag mix Rhizosphere soil samples

TECO ProjectTechnological Eco-Innovations for the Quality Control and the Decontamination of Polluted Waters and Soils

Tel: +39 06 9067 2741E-mail: teco.challenge@gmail.com

Website: www.indoitalianresearch.eu

Activities carried out during the period of the grant• Initiation: General visit to CSIR-NBRI institute, introduction to the Laboratory of

Plant-Microbe Interactions (LPMI), planning the work timeline and bibliographic research.

• Laboratory work:a) CLPP: Biochemical-based method to characterize the metabolic profile of

microbial community’s ability to utilize specific carbon sources.b) DGGE: Molecular-based method to characterize the fingerprinting profile of

DNA sequences coding for 16S rRNA.• Data analyses: Statistical data analyses and interpretation of results• Scientific conference: Presentation of preliminary results at the 1st TECO

Networking Conference (New Delhi, 16-17 November 2016)

CSIR-NBRI Institute Plant-Microbe Interactions Laboratory TECO Networking Conference

TECO ProjectTechnological Eco-Innovations for the Quality Control and the Decontamination of Polluted Waters and Soils

Tel: +39 06 9067 2741E-mail: teco.challenge@gmail.com

Website: www.indoitalianresearch.eu

Main outcomes• Laboratory work:

a) CLPP: Calculation of diversity indexes based on carbon source utilization pattern showed a significant difference among treatments. This is indicative of a shift in microbial metabolic diversity in anthropized soils.

b) DGGE: Clustering analyses of DGGE banding patterns demonstrated the grouping of rhizosphere and bulk samples in different clusters. This is indicative of distinct microbial structure diversity in the rhizosphere.

• Valorization of results through a research article in cooperation between LGE-UPEM (France) and CSIR-NBRI (India)

• CSIR-NBRI as promising hosting Institution for upcoming TECO fellows• Enhancing scientific network between Europe and India• Professional and personal development of TECO participant facing a unique

experience in extraordinary India

CLPP using Biolog EcoPlates ™ DNA extraction and amplification of 16S rDNA Banding patterns in DGGE gels