HIGHLIGHTS.....

� NSL R&D in Biotechnology

Nuziveedu seeds Limited (NSL)

has been consistently ranked as

No-1 Company in the Bio-Agri

category, since 2008, in the

ABLE (Association of Biotech

Led Enterprises) .......

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Training activities at NSL

Development of rice varieties at

Nuziveedu Seeds Limited (NSL)

for increasing rice production

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Development of Rice varietiesat Nuziveedu Seeds Limited( N S L ) f o r e n h a n c e dproductivity and quality

NSLite Corner

Importance Of IsolationDistance In Hybrid SeedProduction Of Cotton

Successful Vegetable HybridsMyna-24, Rajesh, Rambo

Good Practices in HybridPaddy Seed production

1ST AUGUST 2017 | VOL 01 ISSUE 01 MONTHLY RELEASED ON 29TH JULY| | |

GSTGoods and Service Tax

Goods and Service Tax (GST) is supply based destination

tax, which subsumes a large number of Central and

State taxes into a single tax, is meant to mitigate the

cascading effect of taxes, provide near seamless credit and

make way for a common market. GST is dual tax structure

and all transaction of goods and services made for

consideration would attract two levels i.e. Central (CGST)

and State GST (SGST) in case of intra state sales and IGST

(CGST+SGST) in case of interstate sales.

GST is levied on all intra and interstate supplies of goods and/

or services on the value of determined under respective

valuation provisions and at the rate notified by the Central /

State Government and same has to be collected by supplier

of goods or services, however in following cases recipient is

liable to pay tax under reverse charge mechanism (RCM)

instead of collecting by supplier of goods or services

1. In case of specified categories of supply of goods or

services such as services from Goods transport agent

(GTA), Independent advocate etc.,

2. In respect of supply of goods or services or both by

an unregistered supplier to a registered recipient.

New concept of distinct person introduced under GST, A

person can obtain multiple registrations under GST for its

business verticals within state or can obtain multiple

registration in different states for its business as per

provisions of GST or voluntarily and each registration of a

person is treated as separate and distinct person and

transaction between these persons covered under GST as

taxable as per the provisions.

Classification of goods under GST is done with reference to

Custom TariffAct 1975 (51 of 1975). The Harmonized System

of Nomenclature (HSN) mentioned in Custom Tariff Act

adopted for classification of goods under GST and with

respect to services it would be in line with World Trade

Organization classification. As per the present exemption

notification seeds for sowing purpose mentioned in chapter 9

(Coffee, tea, mate and spices) and chapter 12 (Oil seeds and

certain vegetable seeds) are covered.

CFO, NSL

- Sharat Chandra M

GST : Goods & Service Tax

Vision

Mission

Most Preferred Seed Brand. Always.

We align with the changing needs and design our research and

operations to consistently create best value for the farmers

Vision

Mission

Most Preferred Seed Brand. Always.

We align with the changing needs and design our research and

operations to consistently create best value for the farmers

Development of Rice varieties at Nuziveedu Seeds Limited (NSL)for enhanced productivity and quality

During 2001 Nuziveedu seeds (NSL) was making rapid strides in cotton production with the

cotton hybrids developed in its R&D. With an aim to fulfill the needs of rice farmers NSL

intensified research on developing rice varieties and hybrids. The program involved

enriching germplasm by acquiring germplasm lines from public and private organizations and

farming locations in the country. Germplasm Bank has 15041 accessions as on today. This

was followed by intensive hybridization selection and evaluation of breeding lines both at 15-

20 NSL research farms and in the farmers fields as mini kits Promising ones are released to

various states through state of departments of agriculture .NSL varieties are tested in

National field trials simultaneously and those found superior are released and notified by

ICAR and GOI. The varieties identified in different segments suiting to farmers needs and

recommended by R&D in different years are commercialized subsequently at appropriate

times. These are as follows:

Varieties with early duration and medium slender (Super fine grains)

2005-2006: Sonal (MS, 120 days), Sourabh (LB, 130 days)

2006-2007: Super Moti (MS, 120 days)

2009-2010: Moti Gold (MS, 130 days), Sourabh Gold (LB, 130 days)

2011-2012: Indrani (MS, 120 days), NP 209 (LS ,130 days), NP 107-5 (LS ,125 days)

2012-2013: NPH 924-1 (MS, 130 days), PNPH 24 (LS ,120 days)(rice hybrids)

2013-2014: Kanak (SB, 135- 140 days), Kanak Plus (MS, 135-140 days), NP 742 (LS , 130

days)

2014-2015: Suma (LS, 120 days), Suma Gold (LS ,120 days)

2015-2016: NPH 8899(Hybrid), Sampada (SB, 135 days)

2016-2017: Surabhi (MS, 135 days), Fortified with Zinc in kernel.

NSL rice varieties are tested in All India Coordinated Trials each year. Around 167 entries are

tested in ICAR, AICRIP trials and promising ones are released and notified by govt of India.

Till date an area of 12.5 lakh and around 3.75 lakh acres are covered with NSL Varieties and

hybrids respectively in 2016-17 as estimated from the quantities of seed distributed to

farmers. NSL continues to serve farmers needs to increase rice production and productivity in

coming years using conventional and biotechnological techniques and assist India to achieve

ever green revolution.

*Letters in bold Notified by Govt of India

*LB: long bold LS Long: slender MS: medium slender

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Sonal

Surabhi

Sampada

Dr. U. Prasada RaoConsultant - Rice Breeding at NSL

Good Practices in

Hybrid Paddy Seed Production

Good Practices in

Hybrid Paddy Seed Production

Hybrid paddy seed production requires specialized techniques, which need to be thoroughly understood before embarking upon

this venture. The success of hybrid seed production depends on various factors such as choice of field, isolation, seeding time,

planting pattern and weather conditions during the period of flowering, rouging, synchronization in flowering of parental lines,

supplementary pollination techniques, proper harvesting & post harvest management, etc. The optimum sowing window for

hybrid paddy is Nov 10th –Dec 5th, again depends on hybrid & center specific. Important practices to be followed for the

successful production of hybrid paddy seed production are:

250-300 sq. mt (min.) is required for one acre main field, puddle & level nursery area 5-6

days before seeding and make channels to irrigate & drain water. Soak the seeds for 12 hrs and incubate for 36 hrs. Prepare

raised nursery beds of 1mt. Width, broad cast the seed after ensuring the complete sprouting. Apply basal dose of Urea-2Kg.,

DAP-5Kg. & MOP-2Kg. Maintain irrigate & dry conditions up to complete emergence of 2-3 leaf and there after maintain thin film of

water, evening time let in water & morning drain out water-this reduces cold impact.

Keep the field under moist condition for at least 10 days to

allow volunteers to germinate, Thorough Ploughing/ Puddling, Trim the bund for clean cultivation, level the land, transplant 25-30

days old seedlings or 5 leaf age (vary with hybrids), transplanting of older seedlings delay flowering and transplanting of younger

seedlings advance the flowering, double or single seedling with 6:2, 8:2,10:2, 12:2 ratios as per the recommendation, gap filling

within 5 days after transplanting, male border with 2 rows. Basal dose (In puddling), I Top dress (12 DAT), II Top dress (20 DAT), III

Top dress (30 DAT) as per the recommended schedule (100: 40; 40 kg/ha of N, P2O5 & K2O), If Zinc deficiency observes in main

field spray Zinc chelamin @ 100g. /ac, apply 20 kg. Urea to male lines for robust growth at 20 DAT, All top dressings should

complete within 30 days after transplanting

Nursery preparation & Management :

Main field preparation, Transplanting & Fertilizer management :

Nursery beds preparation Nursery at 15 days age

N. Vijaya BhaskarGM, Production, NSL

Main field preparation Transplanting

Water & Weed Management :

Rouging :

Flag leaf clipping :

7DAT (Days After Transplanting) to tillering stage maintain 5 cm. drain the water and dry the field

when female attains maximum tillers to stop unproductive tillers, during tillering to panicle formation keep 5-7 cm. Apply herbicide

3-4 DAT -Top star (Bayer):35g. /ac , Landoxpower (Dupont) 400-500ml. /ac. Submerge the rice field (up to 5cm.) from 7 DAT to

reduce the weed incidence. Minimum two hand weeding at 20 DAT & 40 DAT is required.

need to be done at different stages, At vegetative stage off types with varying plant height, different leaf shape or color

are removed.At initial heading stage, pre flowering & during flowering stage, before harvest – one round of rouging to check, Male,

late volunteers, missed shedders. Shedders generally flower 3-6 days earlier than the female & shedders panicles normally exerts

out of the flag leaf sheath. The anthers of shedders are yellow and plump.

At 5-10% heading stage the upper leaves of the plants are held properly and they are cut with the help of a

sharp sickle in such a way that ½ or 2/3 of the flag leaf is removed. Flag leaf clipping helps in uniform pollen movement and wide

dispersal of the pollen grain to give higher yields. Flag leaf is very important and hence most care is needed while clipping the flag

leaves. Prophylactic spray with any of fungicides after leaf clipping is required.

25 Days after transplanting 35 Days after transplanting

Leaf clipping operation GA3 Application

Supplementary pollination : Rope pulling method for larger size plots – Increase frequency, minimize labor utilization & reduce

cost. Stick method for smaller size plots and where plots are irregular in shape or uneven in topography and where labor is

sufficient. In rope pulling method nylon rope should run parallel to the parental rows. Frequency and efficiency are more important

than methodology. SP frequency at maximum 30min. interval 3-5 times daily until the restorer line ends flowering. Total number of

pollinating days – Minimum of 12-15. Spray water for female at flowering stage in evening to extend stigma receptivity, when

temperature is high.

Supplementary pollination Male harvesting

Harvesting & Post harvest management : Stop the irrigation and drain the water 7 -10 days before harvest. Harvest male plants

first, check the field thoroughly such that no male panicle is left in the male rows. Harvesting to be done when 95% of the grains

turn straw color or golden yellow color. Clean the combined harvester before harvest. Avoid heaping after harvest. Dispatch the

seed when moisture attains below 12%.

Combined harvester while harvesting Drying of the seed on the floor

Nuziveedu Seed Testing Laboratory (IN - 12) is the fifth laboratory

in INDIA which is accredited by ISTA and is the only laboratory in

AP and Telangana states.The laboratory is authorized to issue

Orange International Certificate .

International Seed Testing Association

NSL R&D in BiotechnologyNuziveedu seeds Limited (NSL) has been consistently ranked as No-1 Company in the Bio-Agri category, since 2008, in theABLE(Association of Biotech Led Enterprises) –Biospectrum survey on Biotechnology industry in India.(http://www.ableindia.in/pdf/reports24_10_survey.pdf ). The survey ranking is based on the highest revenues in Agri-biotechcategory, which NSL has accomplished by being having the largest market share in GM Cotton or Bt Cotton in India, the only GMcrop developed by Biotechnology commercialized in India.

The Biotechnology Division which is a part of R&D vision of NSL was started inthe year 2002.Astate of the art Biotech R&D facility was developed which wasalso certified by DSIR in the year 2009. Following are the key researchobjectives of Biotechnology division.

1) Molecular marker (protein & DNA) based quality assessment of cropvarieties and seeds.a. Gene Purity testing of GM traitsb. Genetic purity testing of Plant varieties

2) Molecular Marker Assisted Selection in Plant breeding for fast-trackingproduct (Variety or Hybrid) development process.

3) GM trait development including gene stacking and development of GMcrops in collaboration with public and private sector.

4) Development of insect bioassays for validating insect tolerance based GMtraits.

NSL has been working on insect tolerance GM traits with genes like VIP3a, Cry 1 Ec , Cry 1 C and Cry1Ac prior to GMcommercialization in 2002 in India. The same was also mentioned in their licensing agreement with trait developer in 2004. AlsoNSL is a part of a Public private partnership with Council for Scientific and Industrial Research (CSIR) and a private sectorconsortium for joint development of GM traits in India.

The Biotechnology Division of NSL's R&D has fostered collaborations with international and national institutions like InternationalCentre for Genetic Engineering and Biotechnology (ICGEB), ICRISAT, NBRI, IIRR, CPMB, Osmania University etc. foraddressing various research challenges primarily in applied research relevant to Plant variety development and support to Plantbreeding work with an aim to reduce the cycle time for breeding and product development. The following are externally fundedprojects in progress or completed by the Department.

1. Bio-fortification of Maize with vitamin A and QP (Quality Protein) (DBT-BIRAC funded project)

2. Proposed private sector partnership for gene stacking and traitdevelopment in National Millennium and Technology Leadership Initiative(NMITLI) of Council for Scientific and Industrial Research (CSIR)

3. DBT funded project on drought tolerance in Maize using molecularmarkers in collaboration with ICGEB.

4. CPMB collaboration project on development of insect tolerance forsucking pests.

5. Private sector consortium projects on Seven Stack development for longlasting insect resistance

The Department is conducting independent projects in the following areas.

1. Development and validation of molecular markers for the commercialtraits in focus crops of NSL.

2. Anther and microspore culture and line development3. Tissue culture and genetic transformation of focus crops of NSL.4. Development of Bioinoculants and Biologicals based on Plant-microbe interaction for sustainable agriculture.5. Biotech support to Invivo Doubled Haploidy development in Maize for6. Biotech support to biofortification breeding projects in Rice (Zinc and iron) and Maize (High Lysine).

Since inception the Biotech division has become the pivot for cutting edge research at NSL and has also been providing support toall other organizational functions in conventional breeding, Sup[ply chain management (Grow-out test), quality assurance, etc.The following are the key achievements of Biotechnology R&D division at NSL.

1) Establishment of SOPs for quality assessment of NSLHybrids (Vegetables and Maize) using molecular markers.2) Reduction of breeding cycle times in Tomato by using Molecular markers for development of Virus and Wilt resistant tomato

hybrids3) Development and validation of molecular markers system for GMS (Genetic male sterility) in Cotton and its application in NSL

cotton breeding programmes. The GMS adoption will lead to significant reduction of breeding cycle time by 10% to 20% inCotton parental line development.

4) Development of doubled haploid lines in Chilli and Rice and their utilization in breeding for reduction of breeding cycle time by25%.

5) Identification of Cry 1 Ec and other genes as sources of resistance to Pink bollworm and development of gene stacks forproviding long lasting insect resistance in Cotton.

Dr. M. Satya PrasadChief Scientist - Biotechnology, NSL

Successful Vegetable Hybrids

The preference for salad typed tomato is growing day by day in urban

and semi urban cities in India. Maharashtra, Gujrat and Karnataka are

the states pioneering the production of salad typed tomato. Keeping in

view with the challenges to grow this type tomato as per the traditional

sowing window followed for this type, Nuziveedu Seeds Ltd have

released Tomato-Rajesh. Tomato Rajesh have been crafted with lots

of experiment by the NSL-R&D for its perfection. Rajesh is equipped

with high heat tolerance, excellent shelf life, Tolerant to TolCV,

bacterial wilt. With its attractive red colored 90-100 g fruit with heavy

fruit bearing per plant produces more yield with desired quality.

Rajesh

Vector transmitted disease is becoming a serious challenge for Okra

cultivation in India. High heat with humidity leads to more disease

infestation in Okra leading to serious loss to crop health resulting in

poor yield and loss of expected revenue. Yes, there is solution with

Nuziveedu seeds ltd through its product Hybrid Okra Myna-24 having

dual tolerance for OELCV and YVMV. The Okra Myna-24 having a

strong vegetative growth can withstand the disease pressure and

does deliver superior yield as compared with the competitor. The

name Myna-24 have been started become popular among the

farmers in states like West Bengal, Orissa, AP & TL, Uttar Pradesh

and Bihar. The fresh green tender fruits give a fantastic impression on

crop along with its high level of hybrid vigor.

Myna-24

Watermelon Rambo is a premium innovation from Nuziveedu Seed

Ltd R&D. Inspired from the concept of preference of small watermelon

by the nuclear family Nuziveedu seeds have developed watermelon

Rambo in ice box segment with weight of 2.5 to 3.5 kg. Rambo is

superior in various aspects over its competitor in term of fruit

uniformity, sweetness (13-15% TSS) and rich pulp color. Moreover the

highest level of transportability of Rambo differentiates it from other

products in its segment. Rambo is launched by NSL in all the major

watermelon growing states of India like Tamilnadu, AP & TL,

Karnataka, Maharashtra, Gujrat, Madhya Pradesh, Uttar Pradesh,

Bihar & Jharkhand and West Bengal. Farmers are very much positive

about the attributes of Rambo and hopefully very soon watermelon

Rambo shall take the lions share in its segment.

Rambo

Pranjal BaruahProduct Manager - Veg, NSL

cornerNSLite

Employee development and empowerment of human resources is the core ideology of NSL. As part of this ideology, we have the

half yearly professional development programs are conducted for all the employees force twice a year. The first half yearly

professional development program started in the second week of July 2017. The program aims at training over 600 plus staff.

Given below are glimpses of the training program.

Training Program

Name :

Designation :

DOJ :

Total Experience :

About myself :

Field of

Specialization :

Hobbies :

Professional Experience :

Professional experiences at NSL :

Recognition :

Mahesh N. Sajjan

Principal Breeder (Vegetable)

1/7/2008

15 years

I, Mahesh Nagappa Sajjan born on June 10 th at

Chincholi, Dist: Kalburgi, Karanataka state.

Completed M. Sc.(Agri) from University of

Agricultural Sciences Dharwad. Married to

Rajitheshwari and having two daughters Kum.

Phanisree and Kum. Tanusree.

Vegetable Science (Vegetable

Breeding, Hybrid technology and seed production)

Reading, Exploring new places

15 years of experience in Okra Research &

Development with objective to Develop virus

tolerant (YVMV), high yielding with Dark green fruits

hybrids.

Developed and Released Okra High Virus tolerant

hybrids 1. Rohini(NOKH-1001),2. Classic (NOKH-

1002), 3. Marvel(NOKH-1003), 4.Karishma(NOKH-

1004), 5. NOKH-1012, 6. Simran(NOKH-1013), 7.

Myna-24(NOKH-2002) & 8. Bhagyashri(NOKH-

2003)

Awarded Best Vegetable Breeder award for 2015-

16 In Nuziveedu Seeds Ltd.

Shri M. Gopala Krishna IAS (Retd.) giving a motivationalspeech for participants of Quest 2017Accounting Conference.

Importance Of Isolation Distance InHybrid Seed Production Of Cotton

Importance Of Isolation Distance InHybrid Seed Production Of Cotton

The most important objective of hybrid seed production in

cotton is to maintain the genetic purity of the variety. To

maintain genetic purity the crop raised for seed production

should be separated from the other fields of the same crop

species by a minimum distance.

This minimum distance is known as isolation distance.

Isolation is essential to prevent pollination from unwanted

pollen to avoid chances of cross pollination and to avoid

mechanical mixture. The maintenance of genetic purity of

cotton hybrids depends upon the use of safe isolation

distance.

Cotton is an often cross-pollinated crop, the extent of natural

cross pollination depends upon insect activity. Therefore, the

cotton hybrid seed production plot should have minimum 30

Meters isolation distance on all sides from other cotton fields

for the purpose of keeping seed pure.

Bud Selection Emasculatedflower bud

Removal of corollaand anther sheath

Hand Pollination

Srirama Murthy NGM, Production (Cotton), NSL

ZordarImproved hybird cotton

High Yielding Potential

Big Boll SizeSucking Pest Tolerance

NavaneethImproved hybrid cotton

Early MaturityMedium Boll Size

High Yielding Potential

AkanshaImproved F1 Bhendi

YVMV TolerantHigh Yielder

SurabhiImproved RiceNon Lodging

Tolerance to BPH & Blast

Sy. No. 69, Kandlakoya, Gundlapochampally,Medchal Mandal, Medchal-Malkajgiri District - 501401.

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Disclaimer : The content published in this news letter is subject © copyright. This news letter provides information on internal activities of NSL and it's subsidaries and any source of external information has been duly quoted.

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All India Accountants meet, the conference was themed “Quest-2017- The challenge of change”

“The challenge of change” theme was chosen for the current year's conference as in the past 2 years we witnessed lot of

regulatory changes beginning with INDAS, IFC and GST impacting all corners of our business and throwing a challenge to the

entire Accounting fraternity hence this Accountant's conference gave an opportunity for us to network, bounce ideas off one

another, and learn from the best speakers.

Accountants must continually adapt to cope with the growing complexity of business standards and to update themselves with

all changes brought by regulators. Not to forget it is weACCOUNTANTS MAKE COMPANIES SUCCESS PUBLIC

The conference was structured around a number of presentations where speakers both internal and external shared their

wide ranging expertise with the group of around 100 accountants of our Company.

Editorial Team

Mr. N. Sreenivasa Rao

Mr. Pranjal Baruah

Mr. Gyanendra Herse

Mr. Bipin Acharya

Mr. Rafiq Ahmed

Mr. V. Anil Kumar

Mr. Seshu Babu

Mr. Sridhar Tallam