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100. a. TNF. IL6. 80. 60. Positive cells. (%). 40. 20. 0. CD89. CD16b. CD1a. CD3. CD19. CD14. B. A. LPS. LPS. t tol = 8 h. t rec = 16 h. t st. Analysis (tol). LPS. t st. Analysis (c). D. C. 300. 40. IL12p35/p70. IL10. *. 30. 200. Positive cells. - PowerPoint PPT Presentation
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Gomez-Piña et al., Supplemental Figure S1
A
ttol = 8 h trec = 16 h
LPS
tst
LPS
Analysis (tol)
tst
LPS
Analysis (c)
B
D
*
*
IL12p35/p70IL10
c c + LPS tol + LPS0
10
20
30
40
Po
sit
ive
ce
lls
(%)
C
**
c c + LPS tol + LPS0
100
200
300TNFIL6
[Pro
tein
] (n
g/m
l)
CD89 CD16b CD1a CD3 CD19 CD140
20
40
60
80
100
Po
sit
ive
ce
lls
(%)
Gomez-Piña et al., Supplemental Figure S2
c c+ LPS tol + LPS tol + LPS (GM6001)
0
2
4
6
81
3
6
18
24
48 h
[IL
6/ -
ac
tin
] x
10
-1
c c+ LPS tol + LPS tol + LPS (GM6001)
0
1
2
3
41
3
6
18
24
48 h
[IL
23
p1
9/ -
ac
tin
] x
10
-1
*
* *
*
**
*
*
*
*
* *
A
B
Gomez-Piña et al., Supplemental Figure S3
c s'TREM-1 (50 ng/ml)
c s'TREM-1 (10 ng/ml)
s'TREM-1 (50 ng/ml)
0
1
2
3
436 h
[TN
F
/-a
ctin
] x
10
+LPS
c s'TREM-1 (50 ng/ml)
c s'TREM-1 (10 ng/ml)
s'TREM-1(50 ng/ml)
0.0
0.5
1.0
1.5
2.0
2.536 h
[IL
12
p4
0/ -
act
in]
x 1
0-2
+LPS
+LPS
c s'TREM-1(50 ng/ml)
c s'TREM-1 (10 ng/ml)
s'TREM-1 (50 ng/ml)
0.0
0.5
1.0
1.5
2.0
2.536 h
[IL
10
/
-act
in]
x 1
0-2
A
B
C
**
**
*
**