Genetics of Industrial - GIM 2019...GIM Conference Dinner Date 10 September 2019 Time 20.00 - 24.00 Place Santa Caterina Cloister Piazza Santa Caterina 4 – Pisa Get a chance to spend

14th International Symposium on the

Genetics of Industrial

Microorganisms

Programme

8 - 11 September 2019Pisa · Italy

www.gim2019.org

INDEX

Organisation 4

Welcome 5

General information 7

Information for speakers and poster presenters 8

Social events 9

Programme 11

Sunday 11

Monday 12

Tuesday 14

Wednesday 16

Poster overview 18

314th International Symposium on the Genetics of Industrial Microorganisms

WELCOME

Dear participants, Dear colleagues,

It is a great pleasure to welcome you to the International Symposium on the Genetics of Industrial Microorganisms in Pisa.

This event is organized together with the Italian Society of General Microbiology and Microbial Biotechnology (SIGMBM) and with the support of the GIM International Committee.

During the 3,5 conference days, you will experience a diverse programme that includes keynote sessions, free paper sessions, industry symposia and poster presentations. You will get a unique opportunity to meet experts within the field and be updated on different aspects related to industrial microorganisms.

The scientific programme encompasses a wide range of topics within industrial microorganisms and we hope you find it inspiring.

Furthermore, we have arranged some social events and we hope you will get the chance to experience the historical side of the town.

We hope you will enjoy the conference and your stay in Pisa!

On behalf of the local organising committee and the scientific committee,

Stefano DonadioSymposium Chair

ORGANISATION

GIM International Committee

President:Zixin Deng

Members:Arnold DemainDavid HopwoodDavid WuEric CundliffeGuo-Ping ZhaoJaroslav SpizekJose Luis BarredoJulian DaviesJung-Hye RoeLixin ZhangSang-Ki RheeSergio Sanchez EsquivelTadayuki ImanakaStefano DonadioEriko TakanoHrovje PetkovicRoel BovenbergHilde SchrempfIan MacreadieJuan F. Martin

The Local Organising Committee

Stefano Donadio (Symposium Chair)Emilia GhelardiArianna TavantiFrancesco IannelliMarco VenturaDuccio CavalieriMariagrazia PizzaEzio RiccaPaolo ViscaAlessio MengoniRenato FaniGianni Pozzi

www.gim2019.org

4 514th International Symposium on the Genetics of Industrial Microorganisms

GENERAL INFORMATION

Conference websitewww.gim2019.org

Conference venuePallazzo dei Congressi Di PisaVia Giacomo Matteotti, 1,56124 Pisa PI, Italy

BadgesThe conference name badges must be worn at all times during the conference. Access to the conference venue will not be granted without the name badge issued by the conference organizers.

Entitlements for participants Admission to all scientific sessions and admission to exhibition, coffee breaks and lunch, welcome reception on Sunday 8 September, programme booklet, online access to all conference abstracts and certificate of attendance.

Conference languageThe conference will be held in English.

WIFIFree access to the WIFI at Pallazzo dei Congressi Di Pisa.

Mobile phonesAll mobile phones must be on silent mode during the sessions.

Certificates of attendanceIf you wish to have a certificate of attendance, please log in to the online registration again after the conference and follow the instructions to download your certificate. Should you need any help with this, please contact the secretariat at [email protected]

Lost and foundFound items should be returned to the registration desk. If you lose something, please report to this desk for assistance.

First aidIn case of emergency or need of medical assistance, please contact the Conference Secretariat staff at the registration desk or any of the staff at the venue. First aid equipment is available at the venue.

Conference SecretariatCAP PartnerNordre Fasanvej 113, 2DK-2000 [email protected]

www.cap-partner.eu Tel.: +45 70 20 03 05


INFORMATION FOR SPEAKERS AND POSTER PRESENTERS

SpeakersPlease bring your presentation to the Speakers’ Preparation room at the venue. An assistant will help you upload the presentation to the computer. Please make sure to upload your presentation at least 60 min. before your session starts. Please bring your presentation on a USB stick. We do not allow the use of personal laptops for presentations. At the end of the conference, all presentations will be deleted in order to secure that no copyright issues will arise.

Poster presentersPoster can be mounted from Sunday 8 September at 14.00 and should be mounted by Monday 9 September at 10.00 at the latest. The secretariat will provide all necessary equipment for mounting of posters. Poster should be removed after the last session on Wednesday 11 September.

Kindly note that the secretariat takes no responsibility for left or damaged posters.

Poster sessions are held on:

• Monday 9 September 17.30 - 19.30• Tuesday 10 September 16.30 - 18.30

SOCIAL EVENTS

Welcome ReceptionDate 8 September 2019Time 18.30 - 20.30Place Palazzo dei Congressi de Pisa

Meet old and new colleagues for an informal welcome reception, held at Pallazzo dei Congressi de Pisa at 18.30 - 20.30. The reception is included in the registration fee.

GIM Conference DinnerDate 10 September 2019Time 20.00 - 24.00Place Santa Caterina Cloister

Piazza Santa Caterina 4 – Pisa

Get a chance to spend an evening with colleagues and new acquaintances in an informal atmosphere while enjoying a delicious meal and local wines. The dinner ticket is NOT included in the registration fee – a separate ticket is required.


Sunday14.00 Registration opens16.00-16.30 Opening ceremony16.30-17.30 Plenary Lecture 1:

The CRISPR craze: from adaptive immunity to the genome editing revolutionSpeaker: Rodolphe Barrangou, USAChair: Duccio Cavalieri, Italy

17.30-18.30 Plenary Lecture 2: Theoretical biophysics/Computational systems biologyEdda Klipp, GermanyChair: Wolfgang Wohlleben, Germany

18.30-20.30 Welcome Reception

PROGRAMME 8 September 2019


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Monday08.00 Registration opens9:00-10:00 Plenary Lecture 3: Engineering bacterial envelops for vaccine delivery

Speaker: Rino Rappuoli, ItalyChair: Mariagrazia Pizza, Italy

10:00-10:30 Coffee break & Exhibition10:30-12:30 Plenary - Room 1 Room 2 Room 3

S1: Regulation of secondary metabolism

S2: Microbial enzymes and applications

S3: Microorganisms as drugs

Chairs: Gilles van Wezel,The NetherlandsNadine Ziemert, Germany

Chairs: Marco Moracci, ItalyJozef Anné, Belgien

Chairs: Emilia Ghelardi, ItalyJan Maarten Van Dijl, The Netherlands

10:30-10:55 Omic Insights into Natural Product DiscoveryPaul Jensen, United States

The discovery story of recent transformative enzyme solutions from NovozymesMartin Borchert, Denmark

Pathogen-pathogen inter-actions in the microbiome, a way to soothe virulence?Jan Maarten van Dijl, The Netherlands

10:30-10:4010:55-11:20 Small non-coding RNAs in

Streptomyces coelicolor - important regulators of central metabolism and antibiotic productionBeatrix Süß, Germany

Biotechnological processes with robust microbes and enzymesGarabed Antranikian, Germany

Staphylococcus lugdunensis as probiotic against nasal Staphylococcus aureus colonizationBernhard Krismer, Germany

10:40-11:05 11:20-11:45 De novo identification of

regulatory networks and applications for natural product discoverySebastien Rigali, Belgium

Thermophilic Archaea from genome to function and applicationBettina Siebers, Germany

Engineering microbes to rewire host-microbiome interactionsMatthew Wook Chang, Singapore

11:05-11:3011:45-12:00 Regulatory and mutational ana-

lysis of the biosynthetic gene cluster for ludunomycin, a novel antibiotic with unprecedented chemical architectureHelga van der Heul, The Netherlands

Exploring Novel Bacterial Terpene synthaseGajendar Komati, United Kingdom

Homology-independent pre-diction of subcellular protein localization in beneficial and engineered microbesStefano Grasso, The Netherlands

11:30-11:4512:00-12:15 Systems biology of secondary

metabolites: Deciphering nutrient impact on pamamycin production in a heterologous Streptomyces albus strain J1074/R2Lars Gläser, Germany

Characterization of the gamma-glutamylethanolamide synthetase GlnA4 in Streptomyces coelicolor as a potential industry relevant enzymeSergii Krysenko, Germany

Using yeast to screen for proteostasis inducing drugs to prevent Alzheimer's diseaseIan Macreadie, Australia

11:45-12:0012:15-12:30 Specific 23S ribosomal RNA

mutations conferring erythro-mycin resistance can enhance the frequency of spontaneous mutations in Streptomyces strains perturbing their secondary metabolismKanata Hoshino, Japan

Metagenomics of geothermal environments led to discovery of microbial enzymes for biotechnological applicationsRoberta Iacono, Italy

Antagonistic effects of Lactobacillus Cell Free Supernatants versus uro-genital pathogensMaria Santagati, Italy

12:00-12:15Probiotic Bacillus clausii strains: characterization and potential health benefitsFrancesco Celandroni, Italy

12:15-12:3012:30-14:00 Lunch, Exhibition & Poster viewing

Monday - continued14:00-15:00 Plenary Lecture 4: Small-molecule inhibitors of bacterial RNA polymerase: myxopyronins

Speaker: Richard Ebright, United States Chair: Stefano Donadio, Italy


S4: Challenges in the disco-very of new antibiotics

S5: Vaccine discovery and production

S6: Synthetic biology for fine chemicals

Chairs: Alessandra Polissi, ItalyRichard Ebright, United States

Chairs:Mariagrazia Pizza, ItalyTiziano de Rosa, The Netherlands

Chairs:Francesco Del Carratore, United KingdomEdda Klipp, Germany

15:30-15:55 Deciphering antimicrobial drug interactions using highthroughput approaches Ana Rita Brochado, Germany

Bacterial Vaccines: past, present and future Mariagrazia Pizza, Italy

Boolean Network-based Approaches for Controlling Genetic Networks and Metabolic NetworksTatsuya Akutsu, Japan

15:55-16:20 Natural product discovery in the genomic era: Novel ansamycin antibiotics active on drug resistant tuberculosisJeff Errington, United Kingdom

A manufacturing platform for the production of a vaccine against invasive disease due to extraintestinal pathogenic Escherichia coli Tiziano deRosa, The Netherlands

Designing BiologyRamya Prathuri, United States

16:20-16:45 Next-Generation Fidaxomicin AntibioticsKarl Gademann, Switzerland

GMMA technology: from the development of vaccines for enteric diseases to multivalent vaccines Francesca Micoli, Italy

Mass microscope in microbiologyShuichi Shimma, Japan

16:45-17:10 Unusual and novel antibiotics from actinomycetesSonia Maffioli, Italy

Pathogen-pathogen inter-actions in the microbiome, a way to soothe virulence?Andrea Nayelli Garcia Pérez, Netherlands

16:45-17:00

Production of purple pigment, Violacein and Deoxyviolacein by genetically engineered E. coli.Toshihiko Morita, Japan

16:45-17:0017:10-17:20 A heterologous expression

platform for the production of cryptic lantipeptide antibiotics Abigail Alford, United Kindom

Novel approaches to vaccination: Immunisation with engineered, autologous T-lymphocytes to teach theimmune system how its owncells would appear if infectedby an immunodeficiency virusMauro Pistello, Italy

17:00-17:15

The Discrete Acyltransferase KirCII - A Potential Tool for "Bioderivatization" of Polyketide CompoundsEwa Maria Musio-Kroll, Germany

17:00-17:1517:20-17:30 Mining for antifungals from

bacterial soil isolatesMarija Mojicevic, Serbia

Integrated Probabilistic Anno-tation (IPA): A Bayesian-based annotation method for meta-bolomic profiles integrating biochemical connections, iso-topes patterns and adduct relationships Francesco Del Carratore, United Kingdom 17:15-17:30

17:30-17:40 Setting up in vivo screening of inhibitors of the E. coli Lipo-polysaccharide transport (Lpt) system assembly ElisabeteCardoso Mendez Moura, Italy

17:30-19:30 Poster session & Exhibition17:45-18:15 Explora Biotech Symposium: Bridging the gap between design and synthesis.

Speakers: Davide De Lucrezia & Dario Cecchi.

Programme 9 September 20199 September 2019


Tuesday08.00 Registration opens9:00-10:00 Plenary Lecture 5: Biotechnology of the Human Microbiome - Targets and Therapies for

the TractSpeaker: Willem de Vos, The Netherlands Chair: Marco Ventura, Italy

10:00-10:30 Coffee break & Exhibiton10:30-12:30 Plenary - Room 1 Room 2 Room 3

S7: Physiology and differentiation of industrial microorganisms

S8: Biodegradation and biocontrol

S9: Genome editing tools

Chairs:Ezio Ricca, ItalyEvi Stegmann, Germany

Chairs:Hilde Schrempf, GermanyRadka Chaloupkova, Czech Republic

Chairs:Arianna Tavanti, ItalyTilmann Weber, Weber

10:30-10:55 Allochthonous Bacillus and Their Role in the GI-TractSimon M. Cutting, UnitedKingdom

Next generation bacteriophages for improved biocontrol and diagnostics of pathogenic bacteriaMartin Loessner, Switzerland

A CRISPR-based toolkit to engineer actinomycete genomesTilmann Weber, Denmark

10:55-11:20 Streptomyces coelicolor, surprises from a model organismGilles van Wezel, The Netherlands

The elaborate protein-repertoire supporting the multifaceted biocontrol -strategies of streptomycetes.Hilde Schrempf, Germany

Genome Engineering of Non-model OrganismsHuimin Zhao, United States

11:20-11:45 Metabolomics for the Discovery of New Bioactive CompoundsMargherita Sosio, Italy

Microbial enzymatic systems for converting agro-industrial waste to useful compoundsPimchai Chaiyen, Thailand

CRISPR technologies for genome engineering and evolution of Baker’s yeast Saccharomyces cerevisiae Michael Krogh Jensen, Denmark

11:45-12:00 Streptomyces coelicolor extracellular vesicles carry proteins involved in morpho-physiological differentiationGuiseppe Gallo, Ttaly

Fatty acid degradation in sulfolobus acidocaldariusXiaoziao Zhou, Germany

Direct quantification and localization of efflux pumps in biofilm-associated bacteria through superresolution microscopyTiziano Vignolini, Italy

12:00-12:15 A heat-labile regulatory protein mediates spore coat formation in Bacillus subtitlesRachele Isticato, Italy

Isolation and characterisation of UAE-native microbes with hydrocarbon-degrading and biosurfactant production potentialRunzararoM Nyadzayo, United Arab Emirates

Generation of genomic synthetic integration sites in StreptomycesPedro Albuquerque, Portugal

12:15-12:30 The regulon of the extracytoplasmic function sigma factor SigE in Corynebacterium glutamicumKoichi Toyoda, Japan

Sulfur oxidising bacteria from contaminated dredged sediment: the plasticity of an autochtonous Thioalkalivibrio sp. isolate for the biocontrol of ghas emissionsSimona Di Gregorio, Italy

Crispr-Cas technologies revolutionizing strain improvment : the case of industrial solvantogenic ClostridiaNicolas Lopes Ferreira, France

12:30-13:30 Lunch, Exhibition & Poster viewing

Tuesday - continued13:30-14:30 Plenary Lecture 6: Posttranslational Modifications during Cyclic Peptide Biosynthesis

Speaker: Wilfred van der Donk, United StatesChair: Zixin Deng, China

14:30-16:30 Plenary - Room 1 Room 2 Room 3S10: Biosynthetic pathways

S11: Gut microorganisms and their applications

S12: Targets and leads for antifungals

Chairs:Zixin Deng, ChinaBarrie Wilkinson, United Kingdom

Chairs:Marco Ventura, ItalyDouwe van Sinderen, Ireland

Chairs:Axel Brakhage, GermanyIan Macreadie, Australia

14:30-14:55 Discovery and biosynthesis of new ribosomally-synthesised natural productsAndrew Truman, United Kingdom

Molecular genetics of bifidobacteria: a long and winding roadDouwe van Sinderen, Ireland

New avenues for the discovery and development of antifungal compoundsAxel Brakhage, Germany

14:55-15:20 Basic microbiology, chemistry and synthetic biotechnology to identify and characterize antibiotics from microbesRolf Müller, Germany

Old antifungals: antifolates and boron-containing compounds Ian Macreadie, Australia

15:20-15:45 Aminoglycoside Gentamicins: From Understanding to CreatingYuhui Sun, China

Transfering key genes in Bacteroides to metabolize complex dietary arabinogalactansJose Munoz, United Kingdom

Next generation Antifungal Drug DevelopementDavid Perlin, United States

15:45-16:00 Fungal Biosynthesis of Cyclic Peptides with Unique Chemical PropertiesThomas Schafhauser, Germany

Beyond the tip of the iceberg: illuminating bacterial dark matter through a combination of metagenomics and culturomic approachesGabriele Andrea Lugli, Italy

MAPK signalling pathways cross-talk interaction modulates the production of melanins in Aspergillus fumigatusVito Valiante, Germany

Biocontrol potential of Pseudomonas sp. DSMZ 13134 against the fungal forest pathogens Heterobasidion spp.Martina Pellicciaro, Italy

16:00-16:15 New insights into the biosynthesis of the Gq inhibitor FR900359Max Crüsemann, Germany

The role of TolC outer membrane channel and indole in the regulation of acid resistance in Escherichia coliTakeshi Kanda, Japan

16:15-16:30 Fluorescence-based screening of heterologously expressed biosynthetic pathways in eukaryotesJohann E. Kufs, Germany

The human gut microbiota is shaped by the engraftment of bovine bacteria vectored by milk productsChristian Milani, Italy

16:30-18:30 Poster session & Exhibition20:00-24:00 Congress dinner

10 September 201910 September 2019Programme


Wednesday08.30 Registration opens9:00-10:00 Plenary Lecture 7: In vitro pseudo-natural product biosynthesis and selection

Speaker: Hiroaki Suga, JapanChair: Hilde Schrempf, Germany


S13: Novel bioactive compounds

S14: Aromas and volatiles and their applications

S15: Engineering primary metabolism for strain improvement

Chairs:Olga Genilloud, SpainRolf Müller, Germany

Chairs:Duccio Cavalieri, ItalyPaolina Garbeva, The Netherlands

Chairs:Paola Branduardi, ItalyHrvoje Petkovic, Slovenia

10:30-10:55 Jens Glaeser, Germany Plant terpenes, glycosylation and how to keep them safeRumyana Karlova, The Netherlands

Synthetic strategies for production of aromatic molecules in KluyveromycesmarxianusJohn Morrissey, Ireland

10:55-11:20 Novel inhibitors of the ribosome and protein synthesisDaniel Wilson, Germany

Bacterial aerial clash: volatiles as antimicrobialsMariana Garcia Avalos, The Netherlands

A field of dreams: Lignin valorization into bio-based chemicals and materialsChristoph Wittmann, Germany

11:20-11:45 Novel ansamycin antibiotics with activity towards MDR-Mycobacterium tuberculosisNick Allenby, United Kingdom

The role of yeast in chemical signaling to insectsDuccio Cavalieri, Italy

Biotechnological terpene production – Construction of alternative hosts and expansion of the cellular building block repertoireMarkus Buchhaupt, Germany

11:45-12:00 Investigation of Planomonospora as Specialised Metabolite Producer by Metabolomic ToolsMitja Zdouc, Italy

Harnessing plant-associated bacteria and their volatiles for crop protectionMout De Vrieye, Switzerland

Optimization of the brasilicardin biosynthesis in actinomycetesEvi Stegmann, Germany

12:00-12:15 Novel manumycin-type anti-inflammatory compounds by means of genetic screening and gene shufflingKateřina Petříčková, Czech Republic

Attractive behavoiur of Saccharomyces cerevisiae and Lactobacillus casei on paper wasp Polistes dominulaNiccolò Meriggi, Italy

Bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicumChristina Rohles, Germany

12:15-12:30 New puromycin like metabolites from Escovopsis weberiEdward Hems, United Kingdom

Exploring microbial intersections for discovery of novel volatile compoundsPaolina Garbeva, The Netherlands

Development of Streptomyces to Utilise Sustainable Feedstocks in FermentationsAnna Birke, United Kingdom

12:30-13:30 Lunch, Exhibition & Poster viewing

Wednesday - continued13:30-15:30 Plenary - Room 1 Room 2 Room 3

S16: Bioinformatic tools and big data approaches

S17: Novel industrial microorganisms and approaches

S18: Microorganisms for food and agricultural applications

Chairs:Alessio Mengoni, Italy Marnix Medema, The Netherlands

Chairs:Daniel Lopez, SpainMiaomiao Liu, Australia

Chairs:Roel Bovenberg, The NetherlandsRaffaella Di Cagno, Italy

13:30-13:55 Large-scale discovery of novel strains and species in the global human microbiomeNicola Segata, Italy

Daniel Lopez, Spain How the lactobacilli adapt to and perform in food ecosystemsMarco Gobbetti, Italy

13:30-13:5213:55-14:20 Large-scale genome

sequence analysis and plant-microbe interactionsRebekah Kelly, United States

Pantoea ananatis AJ13355: Genetics, Metabolism and Industrial ApplicationsJoanna Katashkina, Russian Federation

Inspiration for innovation in bakeryFabienne Verté, Belgium

13:52-14:14

14:20-14:45 A roadmap to integrated metabolome and genome mining analysisJustin van der Hooft, The Netherlands

Target Identification by Direct Observation of Protein-Ligand Complexes Using Native Mass SpectrometryMiaomiao Liu, Australia

Microbial small molecules - weapons of plant subversionRonnie de Jonge, The Netherlands

14:14-14.3614:45-15:00 The antiSMASH platform, a web

application and database for mining microbial genomes for secondary metabolite clusters.Kai Blin, Denmark

Exploring the biosynthetic potential of the rare actinomycete Streptoalloteichus sp NAI85712Emilia Palazzotto, Denmark

Biotechnological production of agrochemicals at BASFHartwig Schroeder, Germany

14:36-15.0015:00-15:15 New bioinformatics tools

help the discovery of natural products produced by the actinomycete genus AmycolatopsisMartina Adamek, Germany

Genome-scale model guided study of Planobispora rosea, the natural producer of GE2270A for TOPCAPI project (Thoroughly Optimised Production Chassis for Advanced Pharmaceutical Ingredients)Kamila Schmidt, United Kingdom

High quality soil and insect-associated microbial collections as source of novel natural productsSanja Mihajlovic, Germany

15:15-15:30 Predicting consumer beer preference with artificial intelligence: a lesson on yeast’s important roleSupinya Piampongsant, Belgium

Going smaller : How ‘debugged’ Bacillus subtilis behaves as a cell factoryRocio Aguilar Suarez, The Netherlands

development of omics-based quality control of multi-strain formulations marketed as probioticsRossella Filardi, Italy

15:30-16:00 Coffee break & Exhibition16:00-17:00 Plenary Lecture 8:

Deciphering and exploiting beneficial mycorrhizal fungiSpeaker: Paola Bonfante, ItalyChair: Roel Bovenberg, The Netherlands

17:00-17:30 Closing ceremony & Poster prizes

11 September 201911 September 2019Programme


No. Title Category Final First Name Last NameP1 Streptomyces coelicolor as cell factory for

the production of a metagenome-sourced chitinase with insecticide activity

Biodegradation and biocontrol

Francesca Berini

P2 An unusual type of glycerol-3-phosphate dehydrogenase is involved in glycerol degradation in Sulfolobus acidocaldarius


Christian Schmerling

P3 The role of quorum sensing in degradation of monoaromatic hydrocarbons by Pseudomonas putida AQ8


Simone Di Gregorio

P4 Transcriptomics of bacterial small RNA: improved detection and expression analysis by combining differential RNA-seq and microarray data

Bioinformatic tools and big data approaches

Antonio Rodríguez-García

P5 Development of novel tools for high-throughput genomic and metagenomic analyses - from genomes annotation to resistomes analyses


Mikolaj Dziurzynski

P6 Genome mining and comparative genome analysis to explore Enterobacteria as heterologous hosts for secondary metabolites


Omkar Mohite

P7 Identification of small RNA genes from differential RNA-seq data: a detailed workflow for bioinformatics detection and manual annotation



P8 Using Actinobacteria strains to benchmark genome sequencing technologies and genome assembly strategies


Tue Sparholt Jørgensen

P9 Enzyme-level perturbations by CRISPRi reveal control principles of E. coli primary metabolims


Stefano Donati

P10 Novel strategies for the biosynthesis of protease inhibitors in bacteria

Biosynthetic pathways Leonard Kaysser

P11 Biosynthesis of deoxysugar phosphoramidate metalloprotease inhibitors

Biosynthetic pathways Marius Bader

P12 Identification, cloning and heterologous expression of the gene cluster encoding RES-701-3 and RES-701-4 biosynthesis

Biosynthetic pathways Daniel Oves-Costales

P13 addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

Biosynthetic pathways Juthamas Jaroensuk

P14 Identification of the target and self-resistance mechanism of obafluorin, a ß-lactone antibiotic

Biosynthetic pathways Sibyl Batey

P15 Biosynthesis of the cyclopropyl moiety of belactosin A

Biosynthetic pathways Alicia Engelbrecht

P16 Pseudomonas fluorescens as a production platform for the characterisation and engineering of bicyclomycin biosynthesis

Biosynthetic pathways Natalia M. Vior

P17 Characterization of a type II polyketide synthase (PKS) cluster in the rare actinomycete Kutzneria sp. 1627

Biosynthetic pathways Erika Corretto

P18 structural basis of the pks thioesterase domain directed the engineering of polyene chian release

Biosynthetic pathways Yucong Zhou

No. Title Category Final First Name Last NameP19 Identification and application of

ansamitocin transporters efficiently improved ansamitocin-p3 yield

Biosynthetic pathways Xinran Wang

P20 atp/adp-dependent carbamoylations in the biosynthesis of ansamycins

Biosynthetic pathways Jianhua Wei

P21 Design, tuning and application of antibiotic specific biosensors in Actinobacteria

Challenges in the discovery of new antibiotics

Yuriy Rebets

P22 Screening for antimicrobial activities of Actinobacteria isolated from cave moonmilk deposits


Delphine Adam

P23 Characterization of the cystargolides as protease inhibitors


Nicole Staudt

P24 Engineering the aromatic degradation and transport system of Pseudomonas putida strains for elevated production of Polyhydroxyalkanoates on low-cost substrates

Engineering primary metabolism for strain improvement

Ignacio Poblete-Castro

P25 Engineering glucose transporters and its effect on metabolite production in E. coli


Min-Kyu Oh

P26 Towards 3G bio-production from ocean farms: Systems metabolic engineering of Corynebacterium glutamicum for the production of L-lysine from the major seaweed ingredient mannitol


Sarah Hoffmann

P27 Identification of genes involved in steroid resistence in Mycolicibacterium (Mycobacterium) neoarum by 'omics analyses



P28 Metabolic engineering of E. coli for Oligosaccharides production


Pietro Tedesco

P29 Reconstruction of methanol assimilation pathway in Escherichia coli for methanol utilization


Hirotaka Yajima

P30 Increased Xylitol production by genetically engineered Candida Guilliermondii


Alexander Zwirzitz

P31 One Yeast Strain to Rule Them All: Primary Metabolism Engineering to Obtain Industries Precious Production Strain


Ainsley Beaton

P32 CRISPRi-based titration of transcriptional feedback improves growth of arginine-producing E. coli by balancing overproduction with pyrimidine biosynthesis.


Timur Sander

P33 CRISPR-BEST: a highly efficient DSB-free base editor for filamentous actinomycetes

Genome editing tools Yaojun Tong

P34 Engineering Corynebacterium glutamicum with a comprehensive genomic library and phage-based vectors

Genome editing tools Filipe Marques

P35 Improved Tools for Engineering Streptomyces

Genome editing tools Charlotte Beck

P36 Deletion of FK506 biosynthesis cluster in Streptomyces tsukubaensis by an improved Cas9-CRISPR system

Genome editing tools Antonio Rodríguez-García

POSTER OVERVIEW


No. Title Category Final First Name Last NameP56 Bioactive Amycolatopsis sp. from the

mongolian steppe: identification of secondary metabolites and genome mining yielding novel lasso peptides

Novel bioactive compounds

Jaime Felipe Guerrero Garzón

P57 a hybrid metabolite of isocoumarin and indole alkaloid from an endolichenic fungus aspergillus sp.


Minghua Chen

P58 Rescrutiny of sansanmycin biosynthetic gene cluster leads to the discovery of a novel sansanmycin analogue with more potency against Mycobacterium tuberculosis


Yuanyuan Shi

P59Reactive oxygen species do not contributeapoptosis-like death by gold nanoparticles in Escherichia coli

Reactive oxygen species do not contribute apoptosis-like death by gold nanoparticles in Escherichia coli


Dong Gun Lee

P60 Butyrate protects against high-fat diet-induced atherosclerosis via up-regualting ABCA1 expression in ApoE-deficiency mice


Yu Du

P61 Silent secondary metabolite clusters activation by various regulatory proteins in Actinomycetes


Erik Mingyar

P62 What are the roles of actinomycetes in human microbiome?


Alica Chroňáková

P63 Identification and characterization of the biosynthetic gene cluster of the unprecedented glycosylated lanthipeptide MDN-0207


Marina Sanchez-Hidalgo

P64 Small and macromolecule crystal structures solved with 3D electron diffraction: a new tool in nanocrystallography


Iryna Andrusenko

P65 Structure elucidation by spectroscopic methods and biosynthetic gene cluster analysis of Krisynomycins A-C, potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus


Mercedes Pérez-Bonilla

P66 3D electron diffraction: a single crystal method for nanocrystalline pharmaceutical compounds


Mauro Gemmi

P67 Understanding the mechanism of action of the lanthipeptide NAI-112, an antinociceptive agent


Arianna Tocchetti

P68 Identification and characterization of metabolites produced by Antarctic cold-active bacteria, and their application in various biotechnologies


Michal Styczynski

P69 Caves: ancient and unexplored reservoir for antimicrobial compounds discovery


Daniele Ghezzi

P70 Engineering atypical tetracycline formation in Amycolatopsis sulphurea for the production of modified chelocardin antibiotic


Špela Pikl

P71 Genome Mining of Bioactive Molecules from Nocardia sp. CS682


Dipesh Dhakal

No. Title Category Final First Name Last NameP37 Gene editing efficiently cuts out the

integrated human immunodeficiency virus genome from the host cell genome, but the virus has a chance to start over

Genome editing tools Michele Lai

P38 improved construct of b. breve site-directed mutagenesis vectors

Genome editing tools Emily Hoedt

P39 Berberine Inhibits Choline-Induced Atherosclerosis by Attenuating Trimethylamine and Trimethylamine-N-Oxide production via Manipulating the Gut Microbiota

Gut microorganisms and their applications

Xingxing Li

P40 Characteristics of respiratory microbiota in immunocompromised patients with pneumonia


YEUN-JUN CHUNG

P41 Milk and sugar?: Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal


Kieran James

P42 An engineered Mycobacterium RipA enzyme enhances the vancomycin drug activity against Streptomyces coelicolor

Microbial enzymes and applications

Fernando Santos-Beneit

P43 New screening method for feruloyl esterase producing yeasts suitable for hydrolysis of plant and wood derived materials


Mª del Rosario

Pérez-Redondo

P44 metabolic engineering for the production of hydroxyl amino acids


Lu Shen

P45Identification of quorum quenching bacteriafrom a fish farm and their AHL-lactonase genes


Jung-Kee Lee

P46 Enzymes for industrial vegetable oil degumming


Maria Castelli

P47 A rapid procedure for the in situ assay of periplasmic, PQQ-dependent methanol dehydrogenase in intact single bacterial colonies.


Shreya Shaw

P48 identification of natural inulinase producing bacilli, for industrial applications


Claudia Petrillo

P49 Improved production, translocation and solubility of recombinant protein by low inducer concentration levels in E. coli


Eunyoung Jo

P50 Comparative genomics and deep phenotyping of the plant associated genus Ensifer

Microorganisms for food and agricultural applications

Alessio Mengoni

P51 enhancement of 1,40dihydroxy-e-naphthoic acid production by weissella sp fed-batch culture in protaetia brevitarsis extraction


SunMee Hong

P52 High quality soil and insect-associated microbial collections as source of novel natural products


Sanja Mihajlovic

P53 Application of Plant Growth Promoting Rhizobacteria (PGPR) for the improvement of agricultural productivity


Stefany Castaldi

P54 Anti-HCV Oxazinoindole Alkaloid with an Unprecedented Tetraheterocyclic Skeleton from a Marine-derived Penicillium sp


Maoluo Gan

P55 Discovery of geninthiocin B from new lichen-associating Streptomyces sp.


Olha Schneider


No. Title Category Final First Name Last NameP89 Mapping the regulons of sigma factors

in Streptomyces coelicolorPhysiology and differentiation of industrial microorganisms

Jan Bobek

P90 Role of DegQ-like protease in formation of cell surface structures of Corynebacterium glutamicum

Physiology and differentiation of industrial microorganisms

Masaaki Wachi

P91 Induction of glutamic acid production by copper in Corynebacterium glutamicum


Takashi Hirasawa

P92 Analysis on the phenomenon for the phenotypic diversification of actinomycetes through isolation of their single colonies


Ryo Morimoto

P93 Stress response to butyric acid and butanol formation in Clostridium beijerinckii at transcriptomic level


Petra Patakova

P94 A truncated form of the coprohaem decarboxylase HemQ increases the oxidative stress in Lactococcus lactis subsp. lactis


William Remelli

P95 A peculiar strain of Lactococcus lactis subsp cremoris is able to use citrate as a carbon source


Tiziana Lorenzi

P96 Flow cytometry for better insight into the physiology of sporulating bacteria


Barbora Branska

P97 cytosolic copper affects germination, development and secondary metabolism in streptomyces coelicolor

Regulation of secondary metabolism

Nathaly González-Quiñonez

P98 The sco1897 transcriptional regulator modulates germination, antibiotic production and sporulation


gemma fernández-garcía

P99 Intriguing structural features of the angucycline-like antibiotic auricin and its complex regulation in Streptomyces lavendulae subsp. lavendulae CCM 3239


Jan Kormanec

P100 Characterization of SfbR2, a pseudo-gamma butyrolactone receptor from Streptomyces filipinensis


Eva G. Barreales

P101 Functional characterization of the gene encoding S-adenosyl-L-methionine synthetase in Penicillium chrysogenum


Mª del Rosario

Pérez-Redondo

P102 Role of the PhoP transcription factor on filipin production and morphological differentiation in Streptomyces filipinensis


Jesus F. Aparicio

P103 Subtle genetic adaptations driving pathogenicity behaviour in Streptomyces scabies


Benoit Deflandre

P104 One cluster, different bioactive compounds: bagremycins and ferroverdins are produced by the same biosynthetic gene cluster


Loïc Martinet

No. Title Category Final First Name Last NameP72 Tapping the potential for new bioactive

moleculesNovel bioactive compounds

Jenny Schwarz

P73 Biotechnological investigations of the halotolerant species Aspergillus oleamuriae sp. nov.

Novel industrial microorganisms and approaches

Martina Felli

P74 Exploring the biosynthetic potential of the rare actinomycete Streptoalloteichus sp. NAI85712


Emilia Palazzotto

P75 Construction of a transferable nisin-inducible host-vector system for heterologous gene expression in Gram-positive bacteria


Francesco Iannelli

P76 Industrial applications of different strains of Bacillus spp. isolated from decayed wood



P77 Decayed wood as a new source of bioactive compounds


Mª del Rosario

Pérez-Redondo

P78 Strain improvement of Pseudoalteromonas haloplanktis TAC125 towards its industrial exploitation as cell factory


Concetta Lauro

P79 Dictyostelium discoideum as a source of natural products and expression host for polyketide synthases from amoebozoa


Christin Reimer

P80 Extra-cellular water soluble antioxidant properties of Pseudoalteromonas species according to culture media


Yehui Gang

P81 Investigating the metabolic potential of the rare actinomycete genus Actinospica.


Paolo Monciardini

P82 Antioxidant Properties of Bacillus subtilis subsp. inaquasorum Fermented Algal Biomass; An experiment carried out under saline conditions


Svini Marasinghe

P83 Metabolic flux analysis in Ashbya gossypii using 13C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions


Susanne Schwech- heimer

P84 A role of RNase E/G in transcriptional termination in Corynebacterium glutamicum


Takahiro Kawame

P85 Exploiting the natural solvent tolerance of Pseudomonads for the production of plastic platform chemicals:


Charles Begley

P86 genetics and physiological studies of a thermoanaerobacter kivui mutant lacking hdcr


surbhi jain

P87 An engineered Mycobacterium RipA enzyme enhances the vancomycin drug activity against Streptomyces coelicolor


Fernando Santos-Beneit

P88 Complex regulation of SigB family sigma factors of RNA polymerase in Streptomyces coelicolor A3(2)


Beatrica Sevcikova


No. Title Category Final First Name Last NameP105 Molecular reprogramming of

Streptomyces albus for improved pamamycin production using microparticle enhanced cultivation


Martin Kuhl

P106 Eliciting production of silent antimicrobial metabolites in Streptomyces spp. environmental isolates


Kristiina Vind

P107 Study of Streptomyces sp. carbohydrate catabolic pathways as a means to antibiotic discovery


Sinaeda Anderssen

P108 Total RNA purification from difficult samples: a high-producer mutant of Streptomyces cultivates in industrial conditions


Mª del Rosario

Pérez-Redondo

P109 The DNA cytosine methylome of Streptomyces coelicolor


Rosa Alduina

P110 Regulatory and mutational analysis of the biosynthetic gene cluster for ludunomycin, a novel antibiotic with unprecedented chemical architecture


Helga van der Heul

P111 TOPCAPI: Thoroughly Optimised Production Chassis for Advanced Pharmaceutical Ingredients

Synthetic biology for fine chemicals

Jane Gilsenan

P112 Synthetic biology for the sustainable production of folate


Paola Branduardi

P113 Natural-Vanillin Fermentation from sugar : Production on Industrial Scale


Miku Matsuzawa

P114 Combinatorial Metabolic Engineering Approach for Cyanidin 3-O-glucoside Production in Escherichia coli


Ramesh Pandey

P115 Investigation of biosynthesis of aromatic polyketide antibiotics by synthetic biology


Dominika Csolleiova

P116 Production of antheraxanthin and violaxanthin in E. coli transformed with zeaxanthin epoxydase from plants


Akiko Kubo

P117 Transcriptional cluster "refactoring" to access and expand nature's chemical diversity


Liliya Horbal

P118 Heterologous expression of the GE2270 biosynthetic gene cluster in Streptomyces coelicolor


Oksana Bilyk

P119 Metabolic profile engineering through transcriptional gene cluster "refactoring"


Nikolas Eckert


Rare Biologically Active Antibiotics & Mycotoxins

www.adipogen.com

Unique Structures

Unique Activity Profiles

Lantibiotics

Pseudouridimycin/Rifamycins

Antitumor Agents

Immunosuppressives

Antifungals/Antivirals

Quorum Sensing Agents

BULK Available

Pseudouridimycin/Rifamycins

www.adipogen.com

Highlights at a Glance

Rare Biologically ActiveAntibiotics & MycotoxinsIntroductionThe definition of the term “antibiotics” has evolved and is much broader compared

to the past, when an antibiotic had to be produced by a microorganism and had to

be directed to bacteria or other microorganisms. Today antibiotics include next to

secondary metabolites isolated from microorganisms, semisynthetic derivatives and

chemically synthesized compounds (e.g. sulfonamides), which have antibacterial, anti-

microbial, antifungal and antiprotozoal or similar effects and are potentially useful as

antitumor agents, chemotherapeutic agents, enzyme inhibitors, hypocholesterolemic

agents, immunosuppressive agents, antimetabolites, plant growth modulators, feed addi-

tives, or inhibitors (insecticides, miticides, antiparasitics, phytotoxins, herbicides, etc.).

Antibiotics can be classified based on their mechanism of action (MoA), chemical struc-

tures, mode of production (fermentation, synthetic or semisynthetic), producing or-

ganisms (actinobacteria, fungi (incl. mycotoxins), filamentous bacteria) or spectrum

of activity. Some antibiotics inhibit cell wall biosynthesis, protein synthesis, nucleic

acid synthesis, metabolic pathways or interfer with cell membrane integrity. They also

can be classified by their molecular biological activities (anti-infective, anticancer and

other activities).

CONTENTSIntroduction

1–2Rifamycins 2Pseudouridimycin – UNIQUE! 3Selected Antibiotics (Antibacterial) 3Lantibiotics & Thiazolylpeptides (RiPPs)

4Quorum Sensing – Targeting the Bacterial Biofilm 5

Cancer Research 6–11• DNA/RNA Synthesis | H+-ATPase 6• Immunosuppressives 7• Immunometabolism | Microtubule 8• Proteasome | HSP90 | PP2A 9• Protein Kinase & Enzyme Modulation

10• HIF-1 | Other Anticancer Agents 11• CD1a Ligands 12

Natural Product Library Sets 12Metabolic Syndrome Research 13Inflammation & Neuroscience 14Other Antibiotics, Mycotoxins & Marine Agents 15

Key Research Antibiotics 16

FIGURE: Bioactive metabolites. Adapted from Antibiotics: Current innovations and future trends: S. Sanchez & A.L. Demain (2015)

AntibioticsAntibacterialsAntifungals

Phytotoxins

Enzyme Inhibitors

Herbicides

Plant GrowthRegulators

Antitumor

Antiviral

Feed Additives

Pesticides

Insecticides, Miticides

Antimetabolites

Antiparasitics

Mycotoxins

Immunological Active Agents

Antiparasitics

Tropodithietic acid Page 5

COOHO

S

S

OO

H3C CH3O

HO

OH

H

Heptelidic acid Page 8

O

HN O

O

CH3

Cl

OHHH

Salinosporamide A Page 9

VISIT THE ADIPOGEN LIFE SCIENCES BOOTH AT GIM2019 & GET YOUR FREE COPY

Adipogen_AD_GIM2019_A5h_CMYK_final.indd 1 15.08.2019 13:39:19

Notes

26

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