PACKAGE 1A: Anti-protein purified antibody (expression vector provided by customer)

1. Protein expression and purification• > 90% purity• IPGT induction by three conditions • lead time depends on project

2. Immunization (1 rabbit)• 3 immunizations• guarantee: antiserum diluted 1:30‘000 to recognize 0.1 ug protein antigen in western blot• bleed: at least 40 ml antiserum• 8 weeks

3. Antibody affinity purification• antibody diluted 1:2‘000 to recognize 0.1 ug protein antigen in western blot• purify 40 ml antiserum• 3 weeks

4. Shipment• purified antibody

1. Cloning (cDNA plasmid provided by customer)• primer design• PCR amplification of target• plasmid construction with in-house E.coli expression vector

2. Protein expression & purification• > 90% purity• IPGT induction by three conditions• lead time depends on project

3. Immunization (1 rabbit)• 3 immunizations• guarantee: antiserum diluted 1:30‘000 to recognize 0.1 ug protein antigen in western blot• bleed: at least 40 ml antiserum• 8 weeks

4. Antibody affinity purification• antibody diluted 1:2‘000 to recognize 0.1 ug protein antigen in western blot• purify 40 ml antiserum• 3 weeks

5. Shipment• purified antibody

PACKAGE 1B: Anti-protein purified antibody (including expression vector construction)

GENETEX

CUSTOM ANTIBODY SERVICE

PRODUCT NO: GTX-S-PACK1A PRICE: 1‘680 CHF & 120 CHF SHIPPING

PRODUCT NO: GTX-S-PACK1B PRICE: 2‘130 CHF & 120 CHF SHIPPING

LubioScience GmbH | Baumackerstrasse 24 | 8050 Zürich | 041 417 02 80 | info@lubio.ch | www.lubio.ch

PACKAGE 2: Peptide-specific antibody (unmodified, < 30 amino acid peptide)

1. Free antigen analysis

2. Peptide synthesis (unmodified peptide)• > 75% purity• the end residue must be cysteine• 3-4 weeks

3. Peptide conjugation• 5 mg conjugated to carrier protein• 1 week

4. Immunization (1 rabbit)• 3 immunizations• guarantee: antiserum diluted 1:4‘000 to recognize 0.1 ug peptide antigen in dot blot• bleed: at least 40 ml antiserum• 8 weeks

5. Antibody affinity purification• antibody diluted 1:2‘000 to recognize 0.1 ug peptide antigen in dot blot• purify 40 ml antiserum (3-5 mg purified antibody)• 3 weeks

6. Shipment• purified antibody• remaining peptide

PACKAGE 3: Phospho-specific antibody (one site phosphorylation, <12 amino acid peptide)

1. Free antigen analysis

2. Peptide synthesis (phospho-peptide & non-phospho-peptide)• > 75% purity• > 15 mg• the end residue must be cysteine• 3-4 weeks

3. Peptide conjugation• 5 mg conjugated to carrier protein• 1 week

4. Immunization (1 rabbit)• 3 immunizations• guarantee: antiserum diluted 1:4‘000 to recognize 0.1 ug peptide antigen in dot blot• bleed: at least 80 ml antiserum• 8 weeks

5. Antibody double-specific affinity purification• antibody diluted 1:2‘000 to recognize 0.1 ug peptide antigen in dot blot• purify 80 ml antiserum (2-3 mg purified antibody)• 4 weeks

6. Shipment• purified phospho-antibody• remaining phospho-peptide and non-phospho-peptide

PRODUCT NO: GTX-S-PACK2 PRICE: 1‘990 CHF & 120 CHF SHIPPING

PRODUCT NO: GTX-S-PACK3 PRICE: 2‘400 CHF & 120 CHF SHIPPING