General Microbiology M122

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Big 400 students lectureBiology/Microbiology majors (JR, SR) + nursing students (SO)

GOAL: To gain some insight into the role of microbial communities in human health

In-class

Out-of-classTu

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LEARNING OBJECTIVE: To infer the relationship between human gut microbiome diversity, metabolism and environment

Practice exam questionsReading guide +

pre-reading quizReading guide + pre-reading quiz

Clickers, think-pair-share, group discussion

Learning outcome T Learning activity/Formative assessment Sum asses

To describe how the gut microbiome is profiled and how diversity is measured

Understand

-Reading guide + Pre-reading quiz: sequencing technology and diversity.-Clicker question: select population with the highest diversity.-Think-pair-share: how we can determine taxonomic and functional “abundance” by sequencing”? board/virtual board.-Practice exam questions questions during discussion sections

Multiple choice quiz/exam

To show one example of how microorganism fermentation promotes food metabolism and absorption

Analyze

-Reading guide + Pre-reading quiz: microorganism fermentation in the gut -Group discussion: outline how digestible and non-digestible carbohydrates are metabolized and absorbed, showing cooperation between human and bacterial enzymes board/virtual board.-Clicker question + Think-pair-share: connect sequencing functional data with metabolic pathways. -Practice exam questions questions during discussion sections

To interpret data describing taxonomic and functional diversity of the gut microbiome under different environmental conditions (diet, age, etc)

Evaluate

-Reading guide + Pre-reading quiz: 2-3 figures from Journal articles. Answer questions about hypothesis, what graph is showing, results and conclusion. Bring answers to class.-Group discussion: discuss figures based on the questions they have in reading guide -Clicker + Socratic discussion: questions about experimental design, what figure from paper is showing, results, conclusions-Practice exam questions questions during discussion sections

LEARNING OBJECTIVE: To infer the relationship between human gut microbiome diversity, metabolism and environment

In all the cases shown below, the populations in the upper panel are more diverse than the ones shown in the lower panel

A.TRUEB.FALSE

Turnbaugh et al. 2009. The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice. Sci Transl Med 1, 6ra14

Microbiome plays some role in obesity

-What is a LF/PP diet?-What is a Western diet?-What are humanized gnotobiotic mice?-How did they run this experiment? Explain the timeline (what is in X-axis?)-What are they measuring on Y-axis?-what is acting as control here?

Your research indicates that bacteria that belong to the phylum Firmicutes have high capability to ferment refined sugars and digest fats. Bacteroidetes on the other hand, are better and degrading complex plant carbohydrates. You decide to run an experiment with humanized mice similar to the one we discussed in class: humanized mice growing in a Low fat/High plant carbohydrate diet are switched to a Western diet or kept on a Low fat/High plant carbohydrate diet as control. Based on this, you predict:

A. Bacteroidetes will dramatically increase in the gut of control mice kept in a Low fat/High plant carbohydrate dietB. Firmicutes will increase in the gut of humanized mice switched to a Western dietBacteroidetes will increase in the gut of humanized mice switched to a Western dietC. a and b are correctD. b and c are correct

Our Self Portrait: the Human Microbiome

Reflection of one’s self in dialogue with its host

Oil on canvasTwo panels, 16 x 16 inby Joana Ricou , 2011

They are us, too

Our Courses Self Portrait

Reflection of one’s self in dialogue with its course

Oil on canvasTwo panels, 16 x 16 inby Joana Ricou , 2011

Formative assessment should be there, too