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Fukushimura Brain Bank (FBB) -----Based in a private geriatric hospital---
Hiroyasu Akatsu 1,2), Akira Hori 1,2), Hidechika Okada 1),
Yoshio Hashizume 1,2), Takayuki Yamamoto 1,2)
Conclusions:
Although our bank has gone unrecognized in the past, our farsighted efforts have
been gaining considerable attention in recent years in Japan. We now have over 20
collaborators and supply more than 30 research institutes with our samples. In
addition, our research institute was approved in 2004 by the Ministry of
Education, Culture, Sports, Science and Technology, as one of the non-
governmental institutes which is permitted to apply for governmental grants and
we became a member of the Comprehensive Brain Science Network in 2010. At this
meeting, we present the FBB at the Choju Medical Institute as a unique facility
and one of the most active brain banks in the world.
【Introduction】
The FBB was established under the auspices of the Fukushimura
Hospital, a legally incorporated medical institution. It is managed
completely within the private sector.
1) Choju Medical Institute, 2) Institute of Neuropathology, Fukushimura Hospital
【Fukushimura and Our Hospital】
Choju Medical Institute
Institute of Neuropathology
Fukushimura Hospital
Nursing Home
Home for the
mentally
retarded
Day-care house for the disabled
Home for the disabled
Home for the aged
Vocational aid center
Geriatric Health
Services Facility
Fukushimura Brain Bank was established in 1990. We have a long
record of collecting samples, not only from patients but also from
residents of our care houses and nursing homes within the
Fukushimura complex. This allows us as medical doctors and
researchers to obtain clinical information or blood samples,
sometimes even before the onset of illness. In our institute, all Brain
Bank data is held in the office of individual data management.
【Regarding the sampling section 】
In collecting FBB samples, we always keep in mind future
biochemical and molecular analyses and collaborations. The
brains are separated into two hemispheres. One hemisphere is
fixed in formalin for neuropathological analysis and the other is
precisely subdivided into coronary sections and small blocks
which are saved in Eppendorf tubes. After samples are
photographed, they are frozen on dry ice (slices) and in liquid
nitrogen (tubes). Finally, all material is stored at -80 degrees in 8
refrigerators for later use in research.
The FBB is run by medical doctors in clinical practice with the
assistance of a small technical staff. All cases are regularly
discussed with clinicians on a monthly basis (CPC).
Fixation
Freezing
Tissue from 5
points in the
total brain are
placed into 5
Eppendorf tubes
Fixed blocks of
hemisphere for diagnosis
and smaller portions for
immunochemistry
Longitudinal and Transverse analysis with FBB Samples
Case 1
Case 2
Case 3
Case 4
・
・
Case 500
・
Role of
central institute
Multi-factorial analysis of cases
using the data bank
Collaborative projects
Mass analysis based on
clinico-pathological data
(over 300 cases)
Major on-going collaborations
Kyushu
Univ.
Fujita Health Univ. Hokkaido
Univ.
Shiga Med
School
Hamamatsu
Med. School
Nanyang
Technological
Univ.
Niigata
Univ.
Tsukuba
Univ.
Osaka
Univ.
National
Institute for
Longevity Sci.
FBB
Metals Oxidative
stress
SNPs
Serum/
CSF
SNP: Single Nucleotide Polymorphism
CSF: Cerebrospinal fluid
AD: Alzheimer’s disease
P-102
“Fukushi”is a Japanese word that means welfare and “mura” is a
village. As shown in the aerial photograph on the right, we have several
buildings for the aged and disabled, and about 800 elderly people reside in
our village.
The Fukushimura Hospital was established in 1982 and is managed by the
Sawarabi Medical Cooperative. It currently has 487 beds. Our patients
mainly have dementia and cerebrovascular problems. The hospital plays a
pivotal role within the village and acts as the central facility.
【Fukushimura Brain Bank】
Serum
Mass image
Kyoto
Univ.
Tokyo
Med. Univ.
Basel
Univ.
Hallym Univ.
Fukushima
Med. School
Riken
tau
TDP-43
tau Stroke
AD
Lipids Metals Project x
Clin
ico-p
ath
olo
gica
l da
ta
Role of central institute
Whole body autopsy cases are stored as several
tissues, muscles and spinal cord at -80 degrees
with pathological diagnosis.
Lipids