Fordham Total Application Chemistry

8/14/2019 Fordham Total Application Chemistry

http://slidepdf.com/reader/full/fordham-total-application-chemistry 1/11

Dr. Radhashree Maitra,1580 Pelham Parkway SApt # 1HBronx, NY-10461

The Chairman,

Department of Natural Sciences,

Fordham University,

441 E. Fordham Road,

Larkin Hall 160, Bronx, NY 10458.

12th December 2009

Ref: Application for the position of Assistant Professor – Organic/ Bio-organic Chemistry

Dear Sir,

Please find complied in pdf format my application for the position of Assistant

Professor in the field of organic/ bio-organic chemistry. I have my bachelor’s degree with

chemistry (honors), physics and mathematics and a masters degree in Biochemistry. My Ph.D

.degree is in Biophysics, Molecular Biology and I have several years of research experience

with publications as indicated in my Curriculum Vitae. At Present I am an Assistant Professor at

Albert Einstein College of Medicine of Yeshiva University where I perform only hardcore

scientific research and I am in search of a position which would give me the opportunity to teach

undergraduate and graduate students along with the scope of scientific research. The

multidisciplinary experience that I have gathered over the years gives me confidence that if given

a chance I would become a valuable member of the department of Natural sciences. My list of

three references is attached as an adjoining page to this cover letter.Although I had no formal teaching experience except for my curriculum bound

Teaching assistantship during my graduate studies I have started teaching as adjunct Assistant

Professor at Lehman College CUNY to gather experience and develop expertise.

If my application gathers a favorable consideration I shall be happy to deliver a

talk and discuss my research potentials.

Kindly feel free to contact me for any additional information.

Thank you for your kind consideration.

Sincerely Yours

Radhashree Maitra

.



LIST OF REFERENCES:

1. Dr. Rachel Hazan,Associate Professor

Albert Einstein College of Medicine

1300 Morris Park Avenue

Forchheimer Building, Room 529S

Bronx, NY 10461

Tel: (718) 430-3349

Fax: (718) 430-8541

Email: [email protected]

2. Dr. M.J. Sadofsky,

Associate Professor

Department of Pathology,

Albert Einstein College of Medicine,

1300, Morris Park AvenueBronx, NY 10461

Tel: (718) 430-2222

Email: [email protected]

3. Prof. Ashoke Ranjan Thakur

Vice Chancellor

West Bengal State University Barasat

Berunantukuria, Mallikapur, pin-700126

Tel: 011913325241977 (Off)

011919831179909 (Cell)

011913325241977 (Fax)

Email: [email protected] and [email protected]

mailto:[email protected]











RADHASHREE MAITRA

1580 Pelham Parkway South, # 1H, Bronx, New York 10461. : (718) 801-1359

e-mail: [email protected]

Career Objective

To secure a position with potential of advancement where I can effectively utilize my expertise as

a scientist and educator to work through positive interaction with students to attain global

standards in performance.

Summary of Education

Yea

r

Institution level Subject Grade

1996 Calcutta University Ph.D Biophysics, Molecular Biology & Genetics

1989 Calcutta University MS Biochemistry I

1987 Calcutta University BS Chemistry honors Physics & Mathematics I

Professional Experience

Assistant Professor , Department of Surgery and

Department of Pathology, Albert Einstein College of Medicine,Bronx, NY

2006-2008 NIH training Research Fellow Department of Pathology,

Albert Einstein College of Medicine, Bronx, NY

2002- 2006 Research Associate, Department of Pathology, Albert Einstein College

of Medicine, Bronx NY

2001-2002 Research Associate in Department of Biotechnology, Jadavpur

University, Calcutta, India

1999-2001 Research Scientist in Bioinformatics Calcutta University, Calcutta,

India.

1996.1999 Research Associate Department of Biology Washington University,

St.Louis MO.

Teaching Experience

1990-1996 Graduate Student Department of Biophysics and Molecular

Biology, University of Calcutta, India.

• Teaching three courses of Biochemistry and three courses of Molecular

Biology per semester to undergraduate classes as Teaching Assistant as a stipulated part of the

Ph.D. curriculum of Calcutta University.






Research support NIH training grant NS 07098

Publications

1.Maitra R, Clement CC, Scharf B, Crisi GM, Chitta S, Paget D, Purdue PE, Cobelli N,Santambrogio L. Endosomal damage and TLR2 mediated inflammasome activation by alkane

particles in the generation of aseptic osteolysis. Mol Immunol. 2009 Oct 3. [Epub ahead of

print]

2. Maitra R, Sadofsky M.J. "A WW-like module in the RAG1 N-terminal domain contributes to

previously unidentified protein-protein interactions. "Nucleic Acids Res.2009 Jun; 37 (10):3301-

9.

3. Maitra, R., Clement, C.C., Crisi, G.M., Cobelli, N., Santambrogio L. Immunogenecity of

modified alkane polymers is mediated through TLR1/2 activation. PLoS ONE. 2008 Jun 18;3

(6):e2438

4.. Bunbury, A., Potolicchio, I., Maitra, R., Santambrogio L. Functional analysis of monocyte

MHC class II compartments. FASEB J. 2009 Jan;23(1):164-71

5..Zak, E.A.., Norling, B., Maitra, R., Huang F., Anderson, B., and Pakrasi, H.B. (2001) The

initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes. Proc Natl Acad Sci U S A. 98, 13443-8.

6.Inagaki, N.,Maitra, R.,Satoh, K. and Pakrasi, H.B. (2001) Amino acid residues that are

critical for in vivo catalytic activity of CtpA, the carboxyl-terminal processing protease for

the D1 protein of photosystem II. J Biol Chem, 276, 30099-105.

7.Maitra, R. and Thakur, A.R . (1993) Multiple fragment ligation on glass surface: A

novel approach. Indian Journal of Biochemistry and Biophysics, 31, 97-99.

8. Maitra, R. and Thakur, A.R .(1993) Hydroxyl radical induced DNA damage: choice of

an in vitro model. International Journal of Toxicology, Occupational and Environmental Health. 56, 6-7.

9. Maitra, R. and Thakur, A.R. (1992) Silanisation of glass bound baked DNA permits

enhanced polymerization by DNA polymerase. Current Science 62, 586-588.

http://www.ncbi.nlm.nih.gov/pubmed/19804908?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum











Scientific meetings

•Endosomal damage and TLR2 mediated inflammasome activation by alkane particles inthe generation of aseptic osteolysis Radhashree Maitra, Cristina C. Clement, Brian Scharf,

Giovanna M, Crisi, Sriram Chitta Daniel Paget, P. Edward Purdue, Neil Cobelli, LauraSantambrogio. Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Oral

Presentation. 4th International Meeting on UHMWPE for arthroplasty from powder to debris,

16th -18th September 2009 . Turin, Italy.

• Self-Peptidomic Repertoire of the human pre-nodal lymph.

Clement, Cristina C.; Maitra, Radhashree , Sahu, Ranjit, Santambrogio, Laura. Pathology,

Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Young Investigator Award

talk , 21st American Peptide Symposium, Bloomington, Indiana-July 2009.

• Binding of modified alkane polymers to human recombinant TLR-2 receptor monitoredby intrinsic Tyr fluorescence. Clement, Cristina C.; Maitra, Radhashree Santambrogio,

Laura. Pathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA.

Abstracts of Papers, 236th ACS National Meeting, Philadelphia, PA, United States, August 17-

21, 2008 (2008), BIOL-178.

• Binding of Modified Alkane Polymers to Human Recombinant TLR-2 Receptor

Monitored by Intrinsic Tyr Fluorescence. Clement, Cristina C., Maitra, RadhashreePathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Abstracts,

40th Middle Atlantic Regional Meeting of the American Chemical Society, Queens, NY, United

States, May 17-21 (2008), MRM-259. Publisher: American Chemical Society, Washington,

D. C

• Immunogenecity of modified alkane polymers is mediated through TLR1/2 activation.

Clement, Cristina C.; Maitra, Radhashree; Crisi, Giovanna M.; Cobelli, Neil; Santambrogio, Laura. Pathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA.

Abstracts. 48th American Cell Biology (ACB) National Meeting, San Francisco, December 13-

17, 2008.

• Carboxyl terminal protease of synechocystis 6803 and their role in photosynthesis.

Maitra, R., Ivleva, N.B., Inagaki, N., Satoh, K., Shestakov, S.V. and Pakrasi H.B.

The plant retreat meeting of the Department of Biology Washington University, St.Louis,

Missouri on 2nd May 1998 at trout lodge, Missouri, USA.

• Ctpa a c-terminal processing protease inviolved in the biogenesis of photosystem IIcomplex.

Maitra, R., Inagaki, N.,Anbudurai, P.R. and Pakrasi H.B. The plant retreat meeting of the Department of Biology Washington University,

St.Louis,Missouri on 17th April 1997 at Meramec, Missouri, USA.

• Biochemical activities of glass bound DNA.

Maitra, R. and Thakur, A.R.



Diamond Jubilee Meeting of the SOCIETY OF BIOLOGICAL CHEMISTS (SBC) INDIA on

28th December 1991 at Calcutta.

Honors

•Winner of "SHANTI BHAKTA MEMORIAL AWARD" for the best speaker at the student

seminar held between 15th-21st January 1990 in the Department of Biochemistry ,Calcutta

University.

•Qualified at the "ALL INDIA NATIONAL ELIGIBILITY TEST (NET)"of 1989 in LIFESCIENCES and was awarded scholarship and eligibility to carry out research for the

attainment of doctorate degree from any Indian University or Institute. The scholarship was

valid for five years.

•Qualified for Senior Research Fellowship at all India level in the examinations conducted by

"COUNCIL FOR SCIENTIFIC AND INDUSTRIAL RESEARCH (CSIR) of 1993. The

fellowship was valid for three years.

Membership

•.Member of the "SOCIETY OF BIOLOGICAL CHEMISTS (INDIA)".

• Member of "INDIAN BIOPHYSICAL SOCIETY".

•. Member of “ NEW YORK ACADEMY OF SCIENCE”

Scientific Review

Invited reviewer of Journal of Clinical and developmental Immunology.

Technical skills

• Cell biology and Immunology: Tissue culture, tissue specific typing, isotyping, purification of

monocytes, dendritic cells and macrophages from blood, bone marrow, as well as spleen, thymus

and lymph node. Cell marker specific staining, FACS analysis, ELISA, sandwich ELISA,

immuno-histochemical staining, confocal microscopy, animal immunization, cell proliferationassay, peptide elution.

• Molecular biology: PCR. RT-PCR, DNA cloning vector construction, site directed

mutagenesis, southern blotting, c-DNA library construction and screening, northern blotting, S1

analysis, DNA sequencing, EMSA, Chip Assay, primer extension, tissue culture, transfection,

western blotting. Immunoblotting. Yeast two hybrid assay.

• Biophysics: Spectroscopy, flourometry, circular dichroism, mass spectroscopy, electron

microscopy and HPLC.





Scientific Interest/ Research Plan

My scientific interest is focused on different aspects of molecular recognition processes

especially those that can explain, at the molecular level the pathological conditions in

certain autoimmune diseases like rheumatoid arthritis and artificial implant relatedosteolysis.

My primary investigations at Albert Einstein College of Medicine-Pathology Department,conducted in the laboratory of Professor Laura Santambrogio, are focused primarily on

the role of immature dendritic cells (DCs) in the development of osteolytic necrosis post

artificial prosthesis implantation. We used the mouse model of Ultra High Molecular

weight polyethylene (UHMWPE) induced calvarial osteolysis to follow the precise role played by DCs. We investigated even further the critical contribution of the circulating

DCs in csf-1r -/- knockout mice with severely compromised macrophages and

osteoclasts. The findings are already under journal review. The project was initiated with

in vitro analysis of the effect of modified and unmodified as well as patient retrievedUHMWPE in DC activation. The effects were confirms by electron microscopy

,immunohistochemical staining, surface marker staining followed by flow cytometry,genechip analysis, luciferase assay as well as by FTIR and mass spectrosopy. The

interesting observation that surface oxidized UHMWPE is by far a stronger immunogen

and that Toll like receptor 1/2 play a critical role in the recognition of the non biologic

organic polymer were published in 2008 and was the basis of the previously mentioned invivo studies.

I was also actively involved in the peptidomics and proteomics analysis, utilizing high

resolution mass spectroscopy ESI-LTQ and MALDI-Tof-Tof MS/MS to sequence peptides isolated from the human lymph and determine, for the first time in the field, the

self-peptidomic carrying ability of the lymph. This investigation allowed the discovery of

a new set of peptides that may have immuno-dominant potential, and therefore might beinvolved in the maintenance of peripheral self tolerance or, might stimulate autoimmune

diseases, such as rheumatoid arthritis.

Previous to my work on osteo-immunology I worker with Prof. Sadofsky at the sameinstitution where I was involved in elucidating the precise role of recombinase proteins

RAG1 and RAG2 in V(D)J recombination. During the studies I have identified and

characterized a “WW” like domain in the N-terminal region (NTD) of RAG1 protein that

has high propensity to bind to proline rich region. This might explain the biological roleof the evolutionarily conserved NTD even when it does not directly contribute to the

recombination reaction. During Yeast-2-Hybrid analysis using NTD as bait few preys

were identified and transcription factor GMEB-1 was one of them. I have cloned purifiedcharacterized GMEB-1 and compared its characteristics with well characterized

transcription factor Pax5. The RAG-1 NTD binds strongly to Pax5 and the interactions

have been well characterized.During my research at Washington University I isolated cloned and characterized CtpA a

C-terminal specific protease found in Synecocystis 6803. Site directed mutagenesis was

utilized to identify the amino acids critical for the protease activity. Several temperature



sensitive mutants were generated in the process. Furthermore the membrane biogenesis of

Photosystem 1 was also elucidated.

My graduate studies were done on different structural forms of DNA. The behavior of each structural form of DNA to processing enzymes like topoisomerase, Ligase ,

polymerases and recombinases were extensively studied. SSB or single stranded DNA

binding protein was utilized to evaluate the extent of single strand region present in DNAwhich was further confirmed by fluorescence and fluorescence acrylamide quenching

studies.

The scientific experience and technical expertise that I have gathered over the years givesme the confidence to explore and answer any biological question. All the same I would

prefer to do translational research where I would find the cause and cure of specific

pathological conditions using different biophysical and biotechnological techniques.

Given a chance I would like to continue my investigations in the field of osteo-immunology.



TEACHING PHILOSOPHY

My teaching philosophy is based on a fundamentally optimistic outlook. I believe that

education is a solution to many problems. To educate is to become better educated. It is a

chained process that improves with time. As a teacher I would encourage excellence inmy students and myself. I would urge myself to develop course material that is

challenging and interactive. I strongly believe that using a variety of multimedia

including videos, slides and the internet is an excellent way to compliment traditionalcourse lectures and laboratory exercise. None the less reading coupled with traditional

lectures remains the foundation of the educational experience.

Students learn best when they construct their own knowledge through exploration and

discussion. I wish to use student exploration as an instructional method as it will allowstudents to be active contributors in the classroom and take responsibility for their own

learning. Classroom discussion is an essential part of student education as this is the

space where students use language to synthesize their knowledge and make their work

public. I feel I can encourage students to seek new knowledge and explore their ownideas by an enthusiastic presentation of both what is, and what is not known about the

subject.I believe that all of my students, with the help of the right tools, will be able to overcome

any difficulties that the understanding and analysis of science might present. I do not

mean that every student who enters my class will be able to understand by him or herself

equally simply as a result of how I present the lesson, but rather that each of my studentwill be guaranteed the opportunity to learn and analyze by him or herself to the best of

his or her abilities. I believe that one of my best qualities as a teacher is my ability to

inspire confidence in my students so that they feel comfortable expressing themselvesregardless of their level of ability

In my interaction with students. I will always be respectful, especially when dealing with

differing opinion that may arise as a result of scientific thinking. I have to constantlyrealize that as a teacher I am a facilitator of the process. I want my students to learn to

think critically and not merely accept my ideas and opinion. My drive to pursue higher

education and become a scientist was due in part to several teachers who inspired me andhelped me learn the skills necessary to accomplish these goals. I would like to be the

catalyst for other people to pursue their dreams.



Documents

Fordham Total Application Chemistry