Firestone Center for Restoration EcologyBarú, Costa Rica

Summer 2007Alicia Hill

Purpose of StudyExpand the comprehensive list of all anuran

species present on the reserveDetermine anuran diversity and density at

each pond during dusk timeObtain recordings of individual species

mating callsTrack changes in anuran diversity on the

reserve from the previous year

Why study frog diversity?Indicators of an ecosystem’s healthAbility to track the status of the restoration

progress on the reserve

Previous StudySummer 2006, baseline of anuran species on the reserve14 species were identified, 6 species mating calls were recordedA. callidryas was dominant at dusk, followed by H. microcephala and H. ebraccataOne pond analyzed

MethodsRecorded mating calls at night using an

automated frog logging systemTook six 10 minute recordings every 50

minutesIn this study, only the first 10 minute

recordings (dusk time) were analyzed

Collection SitesFour man-made ponds on the reserveEach pond isolated from one anotherPonds differ in size, surrounding flora, and organisms living in the water

Basilisk Pond

Data AnalysisIdentified each anuran species from the

recorded data at each pondAudacity audio editor and Sigview Signal

AnalyzerCalculated the average calls per

minute(n=10) for each species

Average anuran call frequencies(mean ± SE; n=10) at the four ponds at 6 PM

Data Analysis of H. rosenbergi

Figure 1. Oscillogram of H. rosenbergi, average call frequency is 58 calls/minute

Data Analysis of H. rosenbergi

Figure 2. FFT of H. rosenbergi, dominant frequency at 0.47 kHz

ResultsThe dominant species at this time throughout

the ponds were Agalychnis callidryas, Leptodactylus pentadactylus, and Hypsiboas rosenbergi species (Chi-square test, p<0.0001)

Other ConclusionsMany Duck Pond anurans are mating Anurans doing well in/near bamboo

forest (Creek, Basilisk, Frog)

Summer 2008Continue recording at the ponds and track

species changesSearch for more anuran species that have

been recorded at Hacienda Barú (e.g. Glass frogs)

Expand time recordings to later in the night

Species ListBufo haematiticus

Bufo Marinus

Bufo valliceps

Dendrobates auratus

Dendrobates granuliferus

Agalychnis callidryas

Hyla ebraccata

Hyla microcephala

Scinax elaeochroa

Smilisca phaeota

Eleutherodactylus bransfordii

Eleutherodactylus diastema

Eleutherodactylus fitzingeriLeptodactylus pentadactylus

New Species 2007:Agalychnis spurelliPhrynohyas venulosaHypsiboas rosenbergi

Agalychnis spurelli

Phrynohyas venulosa

Hypsiboas rosenbergi

The prepollex , concealing the prepollical spine

AcknowledgmentsI would like to thank Dr. McFarlane and

Keith Christenson for their guidance and teaching

Thank you to Al Binder, Keala Cummings, Sam Scott, and Emily Shultz for their help.

Questions?