Final Year Project – Seminar Presentation

Final Year Project – Seminar Presentation

Azizan Mohd. Noor

UniKL MICET

Azizan Mohd. Noor

UniKL MICET

1

Why do I need to do well in Presentations?Why do I need to do well in Presentations?

• Phase 1 – 35%

• Phase 2 – 30%

• Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the total marks in the assessment of the project (6 credits) comes from Presentations.

• Presentation is only for 30 min each

• For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks!

• WORTH YOUR WHILE TO DO WELL!

• Phase 1 – 35%

• Phase 2 – 30%

• Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the total marks in the assessment of the project (6 credits) comes from Presentations.

• Presentation is only for 30 min each

• For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks!

• WORTH YOUR WHILE TO DO WELL!2

Prerequisites for a good seminar presentationPrerequisites for a good seminar presentation

• Good researchGood research

• Good understanding of your researchGood understanding of your research

• Well prepared materialsWell prepared materials

• Good researchGood research

• Good understanding of your researchGood understanding of your research

• Well prepared materialsWell prepared materials

3

Preparation of seminar materialsPreparation of seminar materials

• Suitable for the time allocatedSuitable for the time allocated ie. ie. 2020 min min presentationpresentation andand 1010 min min Q&AQ&A

• Suitable medium for presentation – Suitable medium for presentation – Powerpoint being the usual choicePowerpoint being the usual choice

• PPrecise and conciserecise and concise

• Suitable for the time allocatedSuitable for the time allocated ie. ie. 2020 min min presentationpresentation andand 1010 min min Q&AQ&A

• Suitable medium for presentation – Suitable medium for presentation – Powerpoint being the usual choicePowerpoint being the usual choice

• PPrecise and conciserecise and concise

4

Content of a seminar presentationContent of a seminar presentation

• IntroductionIntroduction• ObjeObjecctitive(s)ve(s)• Materials and methodsMaterials and methods• Results and discussionResults and discussion• ConclusionConclusion

• IntroductionIntroduction• ObjeObjecctitive(s)ve(s)• Materials and methodsMaterials and methods• Results and discussionResults and discussion• ConclusionConclusion

5

IntroductionIntroduction

• A brief description of the background A brief description of the background of the researchof the research

• Current status of knowledge in the Current status of knowledge in the area of researcharea of research

• Importance of the researchImportance of the research

• A brief description of the background A brief description of the background of the researchof the research

• Current status of knowledge in the Current status of knowledge in the area of researcharea of research

• Importance of the researchImportance of the research

Intro1 6

An intracellular storage material An intracellular storage material

accumulated by a wide variety accumulated by a wide variety of microorganismsof microorganisms

An intracellular storage material An intracellular storage material

accumulated by a wide variety accumulated by a wide variety of microorganismsof microorganisms

O C (CH(CH2))nn C

O100-30 000

R

Poly(3-hydroxyalkanoate) PHAPoly(3-hydroxyalkanoate) PHA

Intro27

PHA can be divided into:PHA can be divided into:

• Short-chain-length PHA (scl-PHA)

eg: 3-hydroxypropionate, 3-hydroxybutyrate,

4-hydroxybutyrate, 3-hydroxyvalerate

• Medium-chain-length (mcl-PHA)

eg: 3-hydroxyhexanoate, 3-hydrooctanoate,

3-hydroxydecanoate

• Short-chain-length PHA (scl-PHA)

eg: 3-hydroxypropionate, 3-hydroxybutyrate,

4-hydroxybutyrate, 3-hydroxyvalerate

• Medium-chain-length (mcl-PHA)

eg: 3-hydroxyhexanoate, 3-hydrooctanoate,

3-hydroxydecanoate

Intro3 8

The type of PHAs produced depends on:The type of PHAs produced depends on:

• Bacteria strains• Carbon sources• Culture conditions

• Bacteria strains• Carbon sources• Culture conditions

Intro4 9

Some uses of PHAsSome uses of PHAs

• Degradable packaging for consumer products

eg. bottles, bags, films

• Drug-release matrix devices

• Starting material for the synthesis of enantiomeric pure chemicals

• Paint industry – as a binder

• Degradable packaging for consumer products

eg. bottles, bags, films

• Drug-release matrix devices

• Starting material for the synthesis of enantiomeric pure chemicals

• Paint industry – as a binder

Intro5 10

• To screen for local PHA producers

• To optimize the culture conditions

• To manipulate the molecular weight of the polymer(s)

• To screen for local PHA producers

• To optimize the culture conditions

• To manipulate the molecular weight of the polymer(s)

ObjectivesObjectives

11

Materials and methodsMaterials and methods

• Main methods used• Concise – use diagrams or flow-charts if

possible

• Main methods used• Concise – use diagrams or flow-charts if

possible

Materials n methods 1 12

ProcedureProcedureSoil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Materials n methods 2

13

ProcedureProcedure

Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Materials n methods 3 14

ScreeningScreening

Growth of USM4-55 in specific mediumGrowth of USM4-55 in specific mediumGrowth of USM4-55 in specific mediumGrowth of USM4-55 in specific medium

Aerobic fermentationAerobic fermentation(shake flask)(shake flask)

Aerobic fermentationAerobic fermentation(shake flask)(shake flask)

Cell harvestingCell harvesting Cell harvestingCell harvesting

Cell drying (freeze-drying)Cell drying (freeze-drying)Cell drying (freeze-drying)Cell drying (freeze-drying)

GCGCGCGC polymer extractionpolymer extractionpolymer extractionpolymer extraction

Molecular weight analysisMolecular weight analysis

ProcedureProcedure

Materials n methods 415

ResultsResults

• Very improtant

• Concise

• Arranged according to the objectives of the seminar

• Table, graph or photo?

• Very improtant

• Concise

• Arranged according to the objectives of the seminar

• Table, graph or photo?

Results 1 16

  

   

  

   

C/N (mol/mol)

C/N (mol/mol)

Cell dry

weight (g/l)

Cell dry

weight (g/l)

Polyester content (wt%)

Polyester content (wt%)

Polyester compositionPolyester composition

3C43C4 3C63C6 3C103C10 3C83C8 3C123C12 3C12:13C12:1 3C143C14

1010 4.504.50 2525 2222 22 2424 2626 1515 22 1010

1515 3.883.88 2626 2323 22 2424 2525 1010 11 1414

2020 2.922.92 3737 1414 11 3131 3232 99 11 1212

3030 1.601.60 4343 1919 11 2424 3030 1212 11 1313

4040 1.251.25 5050 88 11 2828 3232 1212 11 1818

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55

Results 2 17

  

   

  

   

C/N (mol/mol)

C/N (mol/mol)

Cell dry

weight (g/l)

Cell dry

weight (g/l)

Polyester content (wt%)

Polyester content (wt%)

Polyester compositionPolyester composition

3C43C4 3C63C6 3C103C10 3C83C8 3C123C12 3C12:13C12:1 3C143C14

1010 4.504.50 2525 2222 22 2424 2626 1515 22 1010

1515 3.883.88 2626 2323 22 2424 2525 1010 11 1414

2020 2.922.92 3737 1414 11 3131 3232 99 11 1212

3030 1.601.60 4343 1919 11 2424 3030 1212 11 1313

4040 1.251.25 5050 88 11 2828 3232 1212 11 1818

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55

Results 3 18

Polymer molecular weight profilePolymer molecular weight profile

0 20 40 60 800.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

P(3HB) Mw

P(3

HB

) Mw

, (1x

106 )

Time (hour)

0.2

0.3

0.4

0.5

0.6

0.7

0.8

mcl

-P(3

HA

) Mw

, (1x

106 )

mcl-(P3HA) Mw

Results 4 19

Optimisation of glucoamylase production by Aspergillus nigerOptimisation of glucoamylase production by Aspergillus niger

0 1 2 3 4 5 6 7 8 9 10 11 12

Time (days)

0

10

20

30

40

50

Glucoamylase activity (U/ml)

Initial

1.0% Urea

0.3% YE

5.0% RLGS

pH

Temp.

Results 5 20

Microorganism

• Pseudomonas sp. USM4-55

was locally isolated from the

soil. It was identified by using

API20E.

• Ability to produce biopolymer

detected using Nile-Blue-A

staining method and gas

chromatographic analysis.

Microorganism

• Pseudomonas sp. USM4-55

was locally isolated from the

soil. It was identified by using

API20E.

• Ability to produce biopolymer

detected using Nile-Blue-A

staining method and gas

chromatographic analysis.

Results 621

mcl-P(3HA) P(3HB)

Results 7 22

ConclusionConclusion

• GC is still the better method for screening of new PHA producers

• USM4-55 produces both scl- and mcl-PHA

• USM4-55 can use both glucose and oleic acid to produce PHA

• Polymer accumulation is highly stimulated by nitrogen and oxygen limitation

• Polymer molecular weight can be manipulated by adding certain chemical compounds

• GC is still the better method for screening of new PHA producers

• USM4-55 produces both scl- and mcl-PHA

• USM4-55 can use both glucose and oleic acid to produce PHA

• Polymer accumulation is highly stimulated by nitrogen and oxygen limitation

• Polymer molecular weight can be manipulated by adding certain chemical compounds

23

Effective seminar presentationEffective seminar presentation

• Have a good understanding of the research carried out

• Confidence and poise• Good English• Address the audience• Adhere to the time allocated• Practise!!

• Have a good understanding of the research carried out

• Confidence and poise• Good English• Address the audience• Adhere to the time allocated• Practise!!

24