BIOLOGY Math & Science Dept. Name: _______________________________________________ Yr. & Sec. _____________ Date: _________________________ Teacher: ________________ Group #: _____________ Experiment # ____ The Compound Microscope Problem: _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _______________ Materials: Cut out of letter e form the newspaper Microscope Slide Medicine dropper Cover slip Forceps Slide Procedures: 1. Get a newsprint cut out of the letter e. Make a wet mount of it. 2. Observe the specimen under the low power objective. Compare the position of the letter as seen through the microscope with that of the naked eye. 3. Move the slide to the left and to right. With every movement, observe which way the image of the letter e moves. 4. Now, move the slide forward and backward. Describe the direction of movement by the image 5. Switch to the high power objective. Describe the changes in position and size of the image, the brightness of illumination, sharp and clarity of the image. Put your observation on the table below. Observation: Change in Position Size of the Image Brightness Sharpness & Clarity LPO HPO Guide questions: 1. What difference did you notice when you saw the letter e with your naked eye, under the low power objective and under the high power objective? (you may refer to the data on the table) 2. Why should you look from the side before you lower the objective of your microscope? When you move the slide to different directions, what changes in the specimen under focus did you notice? 3. Explain the effect of opening or closing the diaphragm? SP SCL CPD C CP

Expt. Compound Microscope

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BIOLOGYMath & Science Dept.

Name: _______________________________________________ Yr. & Sec. _____________ Date: _________________________ Teacher: ________________ Group #: _____________

Experiment # ____The Compound Microscope

Problem:______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Materials:Cut out of letter e form the newspaper MicroscopeSlide Medicine dropperCover slip ForcepsSlide

Procedures:1. Get a newsprint cut out of the letter e. Make a wet mount of it.2. Observe the specimen under the low power objective. Compare the position of the letter as seen through the

microscope with that of the naked eye.3. Move the slide to the left and to right. With every movement, observe which way the image of the letter e moves.4. Now, move the slide forward and backward. Describe the direction of movement by the image5. Switch to the high power objective. Describe the changes in position and size of the image, the brightness of

illumination, sharp and clarity of the image. Put your observation on the table below.

Observation:Change in Position Size of the Image Brightness Sharpness & Clarity

LPOHPO

Guide questions:1. What difference did you notice when you saw the letter e with your naked eye, under the low power objective and under the high power objective? (you may refer to the data on the table)

2. Why should you look from the side before you lower the objective of your microscope? When you move the slide to different directions, what changes in the specimen under focus did you notice?

3. Explain the effect of opening or closing the diaphragm?

4. Why should you master the parts and function of the microscope?

5. What are the two basic principles involve in using a microscope?

CONCLUSIONS:

SPSCLCPD

CCP