Introduction to the compound Microscope

Types of Microscopes

Compound Microscope

Stereoscope or dissecting scope

Onion cells (100x) Cheek cells (400x)

Two spotted spider mite (20x)

Types of Microscopes

TEM - Transmission Electron Microscope

Types of Microscopes

SEM - Scanning Electron Microscope

Eye piece

Body Tube

Revolving nosepiece

Objectives

Stage Clips

Stage

Diaphragm

Mirror (light source) Base

Coarse Adjustment

Fine Adjustment

Arm

• Always carry with 2 hands. Place one hand on the arm and one hand on the base.

• Make sure the microscope is on a flat surface and away from the edge of the table.

• Always Look to the side when changing objectives and rotate carefully.

• Always use low (scanning) power first.• Always store the microscope covered and with the

lowest powered objective in place.• Only use lens paper for cleaning

Rules for using the microscope

Microscope Basics: Determining Total Magnification

• Multiply the magnification of the ocular lens (eyepiece) by the magnification of the objective lens.

10 x

4 x

Total magnification = 10 x 4 = 40

We represent this magnification as 40X

Microscope Basics: Field of view

• Field of view is what you see when you look into a microscope.

As you increase magnification your field of view becomes smaller

Microscope Basics: Depth of focus.

• Refers to how much of the specimen you can focus clearly on at one time in your field of view.

• As you increase magnification your depth of focus becomes narrower and less of your sample can be focused at one time.