Department of Biological SciencesVisayas State UniversityVisca,
Baybay, Leyte
Name: Renomeron, Dale Marie P.Instructor: Prof. Marita I.
GalinatoLab. Schedule: MW (1:00 4:00)Date Submitted: Jan. 6,
2013
Exercise 4Smear Techniques: Meiosis
Fig. 1Smeared anther of a Bangka-bangkaan flower bud(Rhoeo
discolor)
Prophase I
Total Magnification: 800x
(Zoomed view)
Fig. 2Smeared anther of a Bangka-bangkaan flower bud(Rhoeo
discolor)
Metaphase I
Total Magnification: 800x
(Zoomed view)
Fig. 3Smeared anther of a Bangka-bangkaan flower bud(Rhoeo
discolor)
Anaphase I
Total Magnification: 800x
(Zoomed View)
Fig. 4Smeared anther of a Bangka-bangkaan flower bud(Rhoeo
discolor)
Prophase II, Telophase I
Total Magnification: 800x
(Zoomed View)
Fig. 5Smeared anther of a Bangka-bangkaan flower bud(Rhoeo
discolor)
Metaphase II
Total Magnification: 800x
(Zoomed View)
Guide Questions:
From which collections (size, stage of development and time of
day) did you observed dividing cells? I observed dividing cells
from the smallest buds which we collected during 12:00 noon. Did
you observe all the stages in a single slide? Unfortunately, I did
not observe all the stages in just a single slide. However, I had a
one slide which contains Prophase I, Metaphase I, Anaphase I, and
Prophase II, Telophase I. But it lacks Metaphase II, Anaphase II,
and Telophase II. And so, I prepared another slide which abled me
to observe only the Metaphase II and still lacking of Anaphase and
Telophase II. What difficulties have you encountered during
preparation of the slide? There were lots of difficulties that I
encountered during the preparation of my slide: 1) It was very hard
to squash the anthers of the flower bud with the use of the base of
the bent needle due to the size of it. When I started smashing the
anthers, some of its particles will stick to the bent needle and
some will no longer be identified due to the acetocarmine stain
that was very red. However, when I already learned about the
technique in which (just like from the onion root tip in Exercise #
3) you must smash the anthers gently with the base of the bent
needle and in order to smashed it more, just put the cover slide
and tap it with the use of the pencil eraser to avoid the breakage
of the slide or cover slip as well.2) Some of my cells are over
stained with acetocarmine stain. But, I only put some of acetic
acid in order to destain my specimen.3) Some of my cells are burned
by the alcohol lamp because of the over-heating. I have to prepare
a new slide all over again.4) It was very difficult to find some of
the stages particularly the Metaphase II, Anaphase II, and the
Telophase II. What specific modification of the standard method
could you suggest for your specific species? Maybe, all I could
suggest of a specific modification of the standard method for my
specific species is that you must know or be familiarized with your
specimen. For example, just like this activity which was the
preparation of smears for meiotic studies. Of course, its important
that you knew of what was the right time of collecting the buds as
well as the right sizes of it to be collected. Through this, you
can possibly observe all the stages that you wanted to observe.
