Environmental Technologies - 東京都産業労働局 · ・Consulting of paper grinding system and bio-plastic production Ibaragi MAPKA® factory Society of Plastics Engineers (SPE)

Environmental Technologies

1. JAG Seabell Co., Ltd.

2. MIRAI-LABO CO.,LTD.

3. Eco Research Institute Ltd.

14

Tokyo Venture Technology Awards

The Potential installation location

Irrigation Canals Water Plants Power Plants Engineered Waterways Off-Grid Development

World’s First Micro Hydro Power Generating System

Ultra Low Head STREAM

15

Corporate Name

Representative Director

Establishment (year)

Capital

Number of Employees

Head Office

e-mail

URL

JAG Seabell Co., Ltd.

(formerly, Seabell International Co.,Ltd.)

Yuji Unno

2004

77,000,000yen

14

2, Rokubancho, Chiyoda-ku, Tokyo,

Japan 102-0085

Tel.+81-3-3237-9634

Fax.+81-3-3288-5882

[email protected]

http://www.jagseabell.jp/english

Tokyo Venture Technology Award

Many installation records

Corporate Profile

JAG Seabell Co.,Ltd. (formerly, Seabell International Co.,Ltd.)

16

New Standard in environmentally friendly power generation

Ultra low head micro-hydropower capturing previously untapped hydro energy in canals and waterways

Patented vertical dual axis Bell mouth inlet feeding kinetic energy into chamber accelerating energy

Compact unit for easy installation No-need for by-pass canal Easy installation, low cost and less environmental impact

Main component of STREAM (in Japan)

Generator

Gear box

Turbine, Base Frame

Electric Control Box

Ready-made (purchase)

Ready-made + Assembly

Welding

Generator Module

Turbine Module

Control Module

Generator Module Turbine Module Control Module + + = STREAM

Localize

Easy localization of technology

•Manufacture •Maintenance

STREAM : the product at a glance


Tokyo Venture Technology Award 17

SECURITY

LIVING LEVEL

INCREASED LITERACY

Depend on manpower

High Cost Fossil Fuel

No light

BENEFIT

PRODUCTIVIT GAINS

COST SAVINGS

JOB CREATION

Irrigation Canal

Stream , Small Waterfall

Rural area Non electrified village

Good solution for rural development

Local Firm

Power producer (Local Gov.

Private companies)

Village group

Villagers

National Gov.

Fund

ODA, loan, grants

Sales

O&M agreement

Improve Power situation

Higher industry And investment

Local vocational training

Employment and Income generation

ODA project

Micro grid power distribution

SEABELL

Tech transfer

Royalty

NGO

Capacity Building

Examples of possible scheme



【 Features 】

• MIRAI-LABO CO.,LTD. has developed a revolutionary

light reflector design for use in LED light washer to

effectively illuminate large areas with uniform lighting.

(3 lux @30m, 30 lux @15m)

• Extreme Energy-saving

(Lowest Power Consumption 18W, replaces 800W

mercury vapor)

• Lightweight - (Around 5Kg, or 11 lbs including battery)

• Makes it possible to hang the light fixture in high locations to provide more

functional illumination around the work areas.

• Eliminates the need of using generators during night construction hours,

eliminating noise,

noise, CO2 production, and safety hazard that come with use of generators.

• Eliminates need of employing dedicated lighting vehicles, reducing labor

and fuel costs.

• Combination use of panel reflectors and parabolic

curve reflectors makes it possible to control

directivity and dispersion of the light output. This

optimizes the use of energy in providing uniform

lighting in distant locations.

• MIRAI LABO owns a patent on usage of panel

reflectors in conjunction with more than 2

parabolic reflectors.

【 Impact 】

【 Principles, Structure, Comparison, etc. 】


X-teraso Rechargeable Battery-Powered Specialty Light Washer

19


Business Overview • Research, development, manufacture, and sales of CO2 reducing systems.

• Research, development, manufacture, and sales of energy-saving LED lighting systems.

• Research, development, manufacture, and sales of natural energy-powered lighting

systems and environmentally adaptive smart street lighting systems.

Corporate Name



Capital

Number of Employees

Head Office

e-mail

URL

Corporate Profile MIRAI-LABO CO.,LTD.

(formerly, MIRAI ENVIRONMENT DEVELOPMENT LABORATORY CO.,LTD.)

Toshio Hiratsuka

2006

100,000,000yen

15

3-3-20 Sennincho, Hachioji-shi, Tokyo, 193-0835, Japan

Tel. +81-42-673-7113

Fax. +81-42-665-1577

[email protected]

http://mirai-lab.com/

MIRAI-LABO CO.,LTD. (formerly, MIRAI ENVIRONMENT DEVELOPMENT LABORATORY CO.,LTD.)

20

MIRAI-LABO CO.,LTD.


• Highrise night-work lighting

Dedicated accessories for street poles allows hanging of the light fixture at any height,

providing effective down lighting to work area.

• Lighting around places where generators cannot be used

Because this MiLED X eliminates the need of generators, it can be used in areas where CO2

emission, noise, or heat emission is restricted.

• Residential area or roadways

By directing and focusing the lighting to the desired lighting area with use of 4 adjustable

arms, light noise and glare to the neighboring residence and moving traffic can be minimized.

• Lighting around noise-sensitive areas

Prevents complaints from residents and increases productivity and improves work

environment.

• Emergency lighting during disters

Highly useful during times of disaster due to its ultra-lightweight and weather proof features

Applications

21

MIRAI-LABO CO.,LTD.


Items Typ. Unit Remark

Size 500×1000 mm Arms opened

Weight 5 Kg Battery Included

Power Consumption 18 W Lamp only

Color Temperature 5100 K Day White

Luminous Flux 2800 lm Total Light Output

CRI 80＜ Ra

Weather Proof Grade IP56 JIS Standard

Illumination Intensity

by Distance

7500 Lx 1m Distance

300 Lx 5m Distance

75 Lx 10m Distance

45 Lx 13m Distance

Continuous Operating

Hours 18/8 Hours

Standard / High

Output

Storage Temperature －40～＋60 °C

Operation

Temperature －20～＋40 °C

Specifications

Light Distribution

22

Paper Powder Reinforced

Plastic Composite “MAPKA®”

【Unique Features】

・New plastic resin with high renewable material content to reduce

fossil-based material and ideal to replace conventional plastics

・Waste paper from paper companies and paper products factories

can be used to dramatically lower carbon footprint

・Proprietary dry paper grinding technology enables 25-50µm powder

creation by which our pellets offer smooth and reliable processing

・Award Winning Unique Technology

Eco Research Institute won the Environmental Award for

Emerging Technologies in Materials from the Society of Plastics

Engineers (SPE)

【Market Impact】

・MAPKA® is the material for the future

- Reduction of petroleum-based material

- Dramatic improvement of carbon footprint

- Wide range of end product applications

- Competitive price by using waste paper

- Improve your brand image and claim bio-based origin

- Recyclable both for material recycling and thermal recycling

- Global need increasing for this new eco-friendly bio-plastic

【MAPKA® Differences】

・Principal ingredients are paper powder and polyolefin (mainly polypropylene)

・Consistent quality for mass production unlike other natural fibers plastic composites

・Shorter cycle & cooling time and lower energy requirements during the molding process

・Designed for injection molding, blow molding, extrusion including sheet and thermoforming

・Available both in master batch form for custom compounders and ready-to-mold thermoplastic

composite pellets for molders

・MAPKA® dramatically reduces your environmental impact


Eco Research Institute Ltd.

Corporate Name



Capital

Number of Employees

Head Office

e-mail

URL

Business Overview

Corporate Profile


・Manufacturing and sales of bio-plastic pellets, principally consisting of paper powder and

polyolefin plastics, designed for injection molding, blow molding, extrusion, sheet and

thermoforming applications

・Manufacturing and sales of paper composites, principally consisting of paper powder and

starch, designed for the manufacturing of steam foamed products

・Manufacturing and sales of steam foamed products including heat insulation boards and

transportation cushions, principally consisting of paper powder, starch and polyolefin plastics

・Manufacturing and sales of equipment designed for the production of paper powder, bio-plastic

pellets, steam foamed heat insulation boards and transportation cushions

・Research & development of recycling of waste paper, plastics and other organic wastes

・Consulting of paper grinding system and bio-plastic production

Ibaragi MAPKA® factory

Society of Plastics Engineers (SPE)

Plastics Environmental Division

Global Plastics Environmental Conference (GPEC)

Environmental Stewardship Awards Winner

(Emerging Technologies in Materials, Processing & Applications)


Takamichi Matsushita

1998

470,000,000yen

181

Green Nanpeidai Building 2F, 16-29 Nanpeidai-cho

Shibuya-ku, Tokyo, 150-0036, Japan

Tel. +81-3-5428-3123

Fax.+81-3-5428-3245

[email protected]

http://www.ecobioplastics.jp

24



Application Examples:

Eco-Friendly Products utilizing unique characters of Paper Powder Reinforced

Composites (MAPKA®)

Low

Shrinkage

Rate

- Comparing with conventional plastics, the pellets

have lower shrinkage rate like engineering plastics

and you can save cooling time

- Cost down by shorter cooling time

- Fit for industrial applications and food packaging

products

Improve

Vibration

Damping/

Sound

Attenuation

-By containing powdered paper, you can improve

vibration damping & sound attenuation

- Fit or electric/electronics appliances and automotive

related products

- Additional modification cost might be incurred

(Ex. motor vibration reduction fabrication)

Improve

Stiffness/

Strength

- Paper powder significantly increases stiffness and

strength of plastics

- Conventional non-organic mineral fillers have a

disadvantage of higher density

- Paper reinforced pellets are much lighter and can

achieve the required properties

Improve

Heat

Resistance

- Paper powder significantly increases heat

resistance of plastics for various applications

- Good for microwave and dish-washer safe

- Paper plastic composites are regarded being

better than polypropylene and non-organic filler

combination for microwavable food packaging

Cup & Lid

Fan Casing

Pallet

Food Trays

25



・This revolutionary breakthrough addresses an emerging global interest in using paper powder

as thermoplastic reinforcing fillers

- ERI’s US subsidiary: Eco Bio Plastics Midland, Inc. (EBPM)

4037 S.Saginaw Rd., Midland, Michigan 48640 USA

TEL：+1 989-496-1934 FAX：+1 989-496-1941

・MAPKA® is now available globally and produced at commercial scale

Left: Taka Matsushita, President with Michigan Governor, Honorable Rick Snyder

Right: Taka Matsushita at Global Plastics Environmental Conference (SPE)

ERI’s technology is Made in Japan but it is rapidly growing globally

(THE CONTACT)

・Americas & Europe: [email protected]

・Japan & Rest of the World: [email protected]

26

Biotechnology

1. euglena Co., Ltd.

2. Synthera Technologies Co., Ltd.

3. Biotherapy Medical Co., Ltd.

4. On-chip Biotechnologies Co. Ltd.

27

The outdoor mass cultivation technology

of microalgae Euglena


・ Achieved the world’s first successful outdoor mass

cultivation of Euglena for food use in December 2005

・ Based on this success, euglena Co., Ltd. started to

sale products to the market and accelerate R&D

activities that collaborate with leading companies and

universities in Japan.

【Features】

【Impacts】 microalgae Euglena

Aviation Biofuel

Euglena contains several nutrients such as vitamins, minerals and proteins, and only has

the cell membrane protecting the cell itself making efficient digestibility possible.

It enabled to make starting sales food items and supplements.

Euglena contains a unique ingredient known as “paramylum”(β-1, 3-glucan). Our company

have researched on the immune system balance adjustment function with paramylum, and

applied it to medical supplies.

Offering a skincare brand cosmetics that has contained “Rejuna ™”, hydrolyzes extract of

Euglena. Offering a pet food and R&D activities for feed.

R&D activities for Aviation biofuel and Biodiesel fuel with fats and oils derived from Euglena. Expanding other R&D criteria.

Outdoor mass cultivation technology of Euglena

euglena Products R&D Activities

Biodiesel fuel

（DeuSEL Project）

Food items &

Supplements

Cosmetics

Pet foods

28


euglena Co.,Ltd.

euglena Co., Ltd.

Mitsuru Izumo

2005

5,424,242,000 JPY

356 (Sep 2017)

5-29-11 Shiba, Minato-ku, Tokyo 108-0014, Japan

Tel.+81-3-3453-4907

Fax.+81-3-5442-4907

[email protected]

www.euglena.jp/

Business Overview

Corporate Profile Corporate Name

President


Capital

Number of Employees

Head Office

e-mail

URL

Through the improvement and development of cultivation technologies, we aim to expand to

lower added value fields, as each of them becomes successively economically viable.

High

Low

Price by weight

Food

Fiber

Feed

Fertilizer

Fuel

The most important factor in euglena Co., Ltd. business

strategy is the concept “5-Fs of Biomass”. This concept is

a set of initial letters taken from the five words ;food, fiber,

feed, fertilizer, and fuel, and the company’s targets at

lowering production cost to that of biofuels.

① Research, development and production of microalgae

including Euglena

② Manufacture and sale of health food and cosmetics

containing microalgae such as Euglena

③ Development of technologies in biofuel production

and environmental management using microalgae

such as Euglena

④ Business development for investment and other

activities in connection with biotechnology – related

businesses

29

mailto:[email protected]



http://www.euglena.jp/






IL-6 standards

0

1

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1.0

10 - 2 10 - 1 10 0 10 1 10 2 10 3 10 4 10 5

MUSTag

ELISA

0

x72

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

IL-8 standards

0

1

2

3

4

5

6

7

8

9

0.0

0.1

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0.9

10 - 2 10 - 1 10 0 10 1 10 2 10 3 10 4 10 5

MUSTag

ELISA

0

x530

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

EGF standards

0

1

2

3

4

5

6

7

8

9

10

11

0.0

0.1

0.2

0.3

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0.7

0.8

0.9

10 - 2 10 - 1 10 0 10 1 10 2 10 3 10 4 10 5

x1700MUSTag

ELISA

0

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

FGF-basic standards

0

1

2

3

4

5

6

7

0.0

0.1

0.2

0.3

0.4

0.5

0.6

10 - 1 10 0 10 1 10 2 10 3 10 4 10 5 10 6

MUSTag

ELISA

0

x17

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

HGF standards

0

1

2

3

4

5

6

7

8

0.0

0.1

0.2

0.3

0.4

0.5

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0.8

10 - 1 10 0 10 1 10 2 10 3 10 4 10 5 10 6

MUSTag

ELISA

0

x24

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

VEGF standards

0

2

4

6

8

10

12

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

10 - 1 10 0 10 1 10 2 10 3 10 4 10 5 10 6

MUSTag

ELISA

0

x290

Ag conc. (pg/mL)

C

t

L

OG

(OD

450 n

m)

Detection antibody

Binding site : Fusion protein

Conjugate oligo DNA to

antibody

Conversion of protein signal to nucleic acid signal

Available to use existing DNA analysis technologies

Ultra-high sensitivity

Simultaneous multiple measurement (3-plex)

Easy-to-use

Wide application

Low price

MUSTag Technology

(MUltiple Simultaneous Tag)

High sensitive protein detection by using real-time PCR

Compare MUSTag Assay to ELISA

4. Measurement of oligo DNA

by using real-time PCR

4. Detection by using plate reader

MUSTag assay kit components

Sensitivity Comparisons

Advantage of MUSTag Technology

Assay reagents

•MUSTag solutions

•Standard antigens

•Buffers

•PCR primers

•Hydrolysis probes

Protocol

(English / Japanese

available)

96-well immunoplate

•Capture antibodies

are immobilized.

20- to 1,000- fold higher sensitivity compared to ELISA.

1. Binding of antigen to

immobilized antibody

2. Reaction of antibody

labeled enzyme

3. Chromogenic /

luminescence reaction

Sandwich ELISA

2. Reaction of MUSTag-Ab.

3. Digestion of oligo

DNA with EcoRI

1. Binding of antigen to

immobilized antibody

MUSTag assay

MUSTag

30


Synthera Technologies Co., Ltd.

Corporate Name



Capital

Number of Employees

Head Office

e-mail

URL

Synthera Technologies Co.,Ltd.

Ippei Watanabe

2005

2,500,000yen

4

1-5-10 Kudanminami Tiyoda-ku

Tokyo,102-0074 Japan

Tel. +81-3-3263-8114

Fax. +81-3-3263-8114

[email protected]

http://www.synthera.co.jp/

Ⅰ．Clinical Diagnostics

A. Cancer Related

1. Bladder Cancer

2. Uterus Cancer

3. Breast Cancer

B. Influenza Related

1. PCR method

2. Immuno- chromatography method

C. Other Applications

1. Alzhemer’s Disease

2. Child Hereditary Disease

Ⅱ．Research Chemicals

1. Ready to Use Chemicals

2. Custom and Order Made Chemicals

Business Overview

Corporate Profile

31

~Sterilized Maggots for Maggot Debridement Therapy~

Medical Maggot


What is Maggot Debridement Therapy ?

Maggot Debridement Therapy (MDT) is a type of wound therapy, in which sterilized maggots

are introduced into wounds to remove necrotic tissue and suppress infection.

Targets of MDT include various types of chronic wounds containing necrotic tissue, such as

diabetic gangrenes, leg ulcers, pressure ulcers, postoperative infectious wounds, and so on.

Photos by Professor Masaaki Miyamoto, Department of Regenerative Medicine, Nippon Medical School

Products Our main products are sterilized maggots for medical use.

Species

We use maggots of greenbottle flies (Lucilia sericata).

There are over 100,000 species of flies on the earth, but only a dozen have

been reported as effective and safe species for medical use.

Across the world Lucilia sericata is one of the most commonly

used species for medical purpose.

Production system

In our laboratory in Nagahama, several

thousand domestic greenbottle flies are

breeding under controlled conditions.

We periodically pick some of them out

of the colony and sterilize them using a

special technique.

Then disinfected maggots are shipped to

various medical facilities.

We also check the existence of aerobic and

anaerobic bacteria in every group of maggots.

Sterelization

Medical facilities

Nagahama laboratory

Production system

A case of

55y.o., Male

Burger’s Disease

before MDT during MDT after MDT

Lucilia sericata

32


Corporate Name



Capital

Number of Employees

Head Office

e-mail

URL

Biotherapy Medical Co., Ltd.

Hitoshi Takase

2006

21,000,000yen

3

1281-8-10 Nagahama Bio Incubation Center, Tamura,

Nagahama, Shiga, 526-0829, Japan

※We have moved our office from Tokyo to Shiga.

Tel. +81-749-53-1485

Fax.+81-749-53-1484

[email protected]

http://www.btmcl.com/

Business Overview

Corporate Profile

Insectary for breeding Clean Room for

sterilization

FED-STD-209D Class10,000

Our company was established in 2006 to provide

domestic medical facilities with sterilized

maggots for medical use. While providing

medical maggots, we store information about the

effectiveness and risk of maggot debridement

therapy, and give feedback to medical

professionals.

We are also carrying out research and

development for new products containing the

secretion of insects, especially maggots.


33

We produce and sell sterilized maggots of

greenbottle flies (Lucilia sericata) for medical use.

According to the differing needs of our customers,

we provide two types of medical maggots.

Free Maggot

Sterilized 1-2-instar maggots of

domestic Lucilia sericata.

Maggots in plastic bottles are delivered to

hospitals, then removed, and

introduced into wounds.

The recommended dose is 5-10 maggots

per cm2.

We also provide specially made mesh stockings, which are useful

in ensuring that maggots do not escape from wounds.

Usually the maggots are left on the wound for 2 to 4 days.

After the treatment, the maggots should be sealed in a plastic bag,

and dumped with other medical wastes.

Photos by Professor Masaaki Miyamoto, Department of Regenerative Medicine, Nippon Medical School

Free Maggot

Maggot Bag

2~3mm

covered with stocking

and gauze

2~4 days

wash out the maggots

Medical Maggot ~Products lineup part 1~



Maggot Bag

Maggot Bags are small pouch-like polyester bags containing 40-300 sterilized maggots .

Maggots Bags have a several advantages over Free Maggots.

First, they help prevent maggots from escaping

from the wound during therapy, which is usually

the highest concern of caregivers.

The second advantage is their usability.

Because Maggot Bags are easy for caregivers to handle,

they can shorten treatment time.

They can also ease the pain that patients feel when

maggots make direct contact with a wound’s surface.

Despite these advantages, caregivers

should keep note that the bags can restrict

some of the maggots’ movement and

that Free Maggots are superior in terms

of effectiveness of treatment.

Maggot Bag on wound

2~4 days

remove from wound

Medical Maggot ~Products lineup part 2~




【Features】

Microfluidic chip based flow cytometric sorter

On-Chip Sort

The world’s first, damage-free & sterile sorter!

■Damage-free sorting

No high pressure, No ultrasonic, No electrical charge

Microfluidics chip for sorting On-Chip sort

Non-sorting

Ａ

Sorter A

B

On-Chip Sort

C Dead and/or non-functioning cells

when sorted by conventional sorter

A (B),

Live neuronal cells in non-sorting

(A) and sorted by On-Chip Sort (C).

■Contamination-free sorting

Fit in a biosafety cabinet. Measurement using disposable chip

■Sorting in the culture medium

Sheath and sample buffers can be any buffer, such as the culture medium.

■Small size and maintenance free

State-of-the-art technology made it possible small size sorter. Disposable chip enables

wash/maintenance free operation.

■Sorting of precious samples

Sample volume is 10～100ul, the best way to scale down the assays.

Neuronal cells from mouse embryonic hippocampus 7days after sorting.

Biosafety and damage are big issues for flow cytometric sorting. However, the

conventional jet-in-air cell sorters damage the cells severely. We developed a

disposable microfluidic chip based cell sorter. The sorting is done by pulse flows, and

this brand-new technique is free from any main damage, such as high pressure,

ultrasonic and electrical charge.

36

On-chip Biotechnologies Co. Ltd.

Corporate Name



Capital

Number of Employees

Head Office

e-mail

URL

Business Overview

Corporate Profile


“ The World’s First! Cell sorter using disposable Microfluidic Chip ! ”

The conventional Cell sorter is expensive, large size and high cost of maintenance device

which requires extensive training for correct usage. We provide innovative Cell sorter that is

easy to operate, small size and maintenance-free. On-Chip Sort is designed the consolidated

hydrodynamic focusing system by utilizing disposable Microfluidic chip

Products

Microfluidic chip Flow cytometer “On-chip Flow”

The World’s First! Flow cytometer using disposable Microfluidic Chip

Microfluidic chip Cell sorter

Microfluidic chip Cell sorter “On-Chip Sort”, which is able to damage-

free & sterilized sorting.


Masayuki KOBAYASHI

2005

100,000,000yen

14

204 Tama-Koganei Venture Port ,

2-24-16 Naka-cho, Koganei-shi, Tokyo, 184-0012, Japan.

Tel. +81-42-385-0461

Fax.+81-42-385-0462

[email protected]

http://www.on-chip.co.jp

37



On-chip innovation

Microfluidic technology Disposable chip-sterilized & cross-contamination free

Chips for both cell analysis and cell sorting are available (Fig 1),and each of them have choice

of sterilized or non-sterilized chips. The size of chip are 5.5cm x 4.0 cm and they are made

from an acrylic resin (PMMA). The width of the microfluidics is 80 micrometers, and the depth

of it is from 50 to 80 micrometers. Whole fluid system including sample, sheath, and waste flow

paths are fit within the chip, which prevents contamination into the equipment. The process is

free from any aerosol.

In addition, On-chip Flow/Sort are compact to fit in a

bio-safety cabinet, providing the best solution to deal

with biohazardous samples (Fig2).

Easy operation and maintenance-free

Free from a time-consuming maintenance of the equipment. On-chip Flow/Sort do not have

any wet tubes due to the use of disposable microfluidic chips. It is a semi-closed system with

the use of attached chip holder covering the chip. After discarding the chip at the end of

analysis or sorting, operation is terminated by simply turning off the power (Fig3).

The operation is very simple as droplet formation nor electrode sorting method is not adopted.

Chip Loading the sample Set in the holder Installation Run

Chip for analysis Chip for sorting

Fig1. Disposable microfluidic chip Fig2. On-chip Sort installed in bio safety cabinet

Fig3. The microfluidic chip enables a closed system

38



Sorting technology Achieve a damage free sorting

The conventional cell sorter damages the cells by the high speed collision (10m/sec) to the surface

between air and water, high pressure flow control (>45PSI) and electric charging at the time of cell

separation.

On-chip innovation

Flow system technology Flow control & a wide selection of sheath liquid sample solution

Sheath and sample flow in micro channels are

controlled as non-contact flow system by air

pressure formula. During the sorting, the target cells

are collected into the collection reservoir. In addition,

cells are also recoverable form the waste reservoir.

For the sorting, the separation is carried out by

pulse flow in micro-channels. Various sheath liquid

and sample buffer are enabled for the assay, such

as the culture medium with serum and even oil.

Fig4. Flow and optics of microfluidic

Micro flow paths Microfluidics chip

Target

Sort

Detection

PUSH PULL

Non-target

80μm

Fig5. Principle of sorting

On the contrast, On-chip sort causes no

damage to the cells because the flow speed is

less one m/sec without the collision, the flow

control pressure is less than 0.3PSI, sorting

force is not electric field but pulse flow.

Furthermore, the sorting is carried out by

choices of buffers to prevent damage to the cell.

When the target cell is passing through the

sorting area, push/pull pulse flow is generated

in a short moment. This pulse flow can separate

the target cell into isolation reservoir. (Fig5).

Main specifications

Sorting method “Flow Shift method”

Laser Up to 3 Lasers

Volume of sample 10～100μL

Purity 98%

Yield >80%

Cell damage No

Contamination No

Fluorescent sensitivity <200 MESF FITC

Detection speed 4,000 events/sec

Sorting speed 100 target cells/sec

Start-up 5 minutes

Size (W,H,D) 620,330,390mm

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Documents

Environmental Technologies - 東京都産業労働局 · ・Consulting of paper grinding system and bio-plastic production Ibaragi MAPKA® factory Society of Plastics Engineers (SPE)