Eastern Africa Regional Laboratory Eastern Africa Regional Laboratory Network (EARLN)- FMDNetwork (EARLN)- FMD

Prepared By: S.N. Wekesa and C. Ngeleja (Coordinators EARLN-FMD)

EARLN-FMD 12 Countries and Contact PersonsCountry Name of Animator Designition/Lab. Station

Kenya Dr. Sabenzia N. Wekesa Vet. FMDL/Coordinator

Tanzania Dr. Chanasa Ngeleja M Vet.CVL/Asst coordinator

Uganda Dr. Chris Ayebazibwe Vet/NADDEC

Ethiopia Drs Gelagay/Tesfaye Vets/NVI/NAHDIC

Burundi Dr. Lazarus Butunungu Vet/Lab

Sudan (N) Dr. M. Habiela Vets/CVRLab

Somalia Dr M Hassan Ali Jabra SAHSP National Epidemiologist

S. Sudan Dr. David Ajwok Vet/admin

Rwanda Dr David Kiiza Vet/Lab

Somalia Dr. Mohamoud Jabra Vet Epidem/SAHSP

Eritrea Dr. Tesfaalem Sebhatu Vet/NVL

Djibouti Said Waiss Miguil Tech/NLDAD

DR Congo Dr Bushu Mulinda Vet/Lab

EARLN-FMD

EARLN- FMD 2010• Support FAO, EuFMD 2010-12, Govts support• First meeting in 2010 Sudan, Ethiopia, Somalia.

Kenya, Uganda, Burundi, Tanzania (7 countries)• Sharing information on country’s FMD activities • Field-based foot and mouth disease outbreak

investigation: – Biosecurity– using Participatory epidemiology (PE) tools– the importance of lesion aging for selection of animals

for sampling and for establishing timeline of events

Participatory Epidemiology

Biosecurity

EARLN-FMD 2010 cont• FMDV diagnosis

– Window of detection by different techniques/tissues– Preservation and transportation

• The role of FMD Reference Centres (RCs) and lab networks in relation to Regional Roadmaps and the Progressive Control Pathway (PCP) for FMD

• Presentations and field activities – Nairobi and Nakuru

• Proficiency tests organized Kenya, Uganda, Ethiopia 2010

• 2011 – meeting in Ethiopia

• Sampling technics to Vets and Technicians in Burundi

• Tanzania EUFMD/FAO RT-PCR (conv and rt) and IZSLER ELISA to all network member countries• Batch 1 (N.Sud, Eth, Djb, Ken, Ug, Rwn, Bur, Tz) done• Batch 2 to be conducted later

• Vaccine matching to Kenya (2 Vets trained in Nov.2011) Embakasi – virus isolation and conc, VNT & LPBE done, RT-PCR setup – partially completed

• Series of Nakuru Training Courses (NTC) to Kenya and other FMD free countries – 7 within 2 years

EARLN-FMD Activities in 2011 - trainings

2011 activities (cont)

• Regional newsletter• FMD field and lab manual• Monthly and annual reports • Assessment of lab capacities• Interactions network:

• e-mail, EUFMD Wiki-space, Phone calls and annual general meetings

Serotypes detected

Country 2009-2010 2011N. Sudan O and SAT 2 O and SAT 2Ethiopia O and SAT 2 O and SAT 2Somalia O, A, SAT 1 and

2O, A, SAT 1 and 2

Kenya O, A, SAT 1 and 2

O, A, SAT 1 and 2

Burundi O, A, SAT 1 and 2No lab Dx prior 09No vaccinations

SAT 1Vaccinations initiated

Uganda A, O and SAT2 O and SATs in wildlifeTanzania A, O and SAT2 ???

Vaccine matching and used vaccinesCountry O A SAT 1 SAT 2Ken & Bur (10/11)

OK77/78LPBE 0.54

AK 5/80 T155/71(RHO VNT 0.51, 0.95 LPBE 0.75 0.25)

K52/84(K65/82 LPBE 0.11)

Eth NVI (local strains)X India (O manisa & A22

Sud Serotype O/SUD/2008 (antigenically close to Manisa, BFS 1860 & Ind R2/75 using VNT at IAH-09

Bivalent O and SAT 2

Ug Trivalent Vacc: O, SAT 1, SAT 2 (ex Kenya)

Tz Vacc used undocm

VNT Ind R2/75 (>1), Manisa (0.39), Taw98 (0.31) LPBE K77/78 (0.50) Manisa (0.53)

Eritrea (0.37 & 0.52), A22 Irq (0.14 & 0.33) Sau 41/91 (0.19 & 0.38) Tur06 (0.1 & 0.32)

Vac mat Eri (0.38)

Lab capacities in 2011Country BSL TestsN.Sudan 2 Ag and Ab ELISAS. Sudan ?? ELISA, VNTEthiopia 2 VI, Ag and Ab ELISA, PCRSomalia ?? VI, VNTUganda 2 VI, Ag ELISA, SPBEKenya 2 VI, Ag ELISA, VNT, NSP ELISATanzania 2+ Ag ELISA (IZSLER and IAH), LPBE, NSP

ELISA, PCR (conventi. & rt)

• Regional activities– Participated in regional meetings on PCP- Botswana

March 2011– Laboratory network activities– Networking with the EA Epidemiology network

• Collaborative projects and partners -EUFMD, FAO, WRL-Pirbright

• FMD control programmes in region– lab involvement – Surveillance, Diagnostics, Vaccine

matching, vaccine quality assuarance.

Regional ActivitiesRegional Activities

Workplan for 2012Workplan for 2012Continued support for network activities and

meetingsFormation of research sub-groups within networkSharing of information across networks in the regionContinuous training in lab. test Support for upgrading of labs in the region and

accreditation of a reference lab in EAWider participation in PTsSupport in acquisition of lab equipment and reagents

Workplan for 2012Workplan for 2012Suggestions for presentation in Bangkok 2012• Results of Survey on Lab capacity in EA region• Comprehensive Review of FMD Epidemiology in the

EA region• Activities of the EA network towards PCP

• the project to develop serotype-specific primers for EA FMD has been approved by EuFMD and withinin 12 months should evaluate primer sets for use in EA, (IAH and Lindholm, with local partners in Uganda and Tanzania. Actions: mainly IAH/Lindholm to initiate

Workplan for 2012 (cont)• annual meeting (tentatively) the 5-6th March, in

Nairobi (back to back with the FAO/OIE/AU regional PCP/Roadmap Development Workshop). Action: mainly from EuFMD - to confirm dates.

• the annual surveillance report for the OIE/FAO Annual Lab network report (on virus circulation in the EA region in 2011 - based on national confirmations and international ref lab findings) should be finalized in early Feb (the deadline is usually set by Pirbright, and allows for completion of lab results for outbreaks to the end of December). Action: Sabenzia/Chanasa

THANK YOU