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PRESENTATION 25.09.2019
PhD student name. DAVIDE BIANCHI
Cycle: XXXIV
Supervisor. OSVALDO FAILLA
Title of the project:
GRAPEVINE ROOTSTOCK CHARACTERIZATION FOR DROUGHT TOLERANCE
Keywords:
Viticulture, rootstock selection, water stress
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
OUTLINE OF THE TALK
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
1. Introduction
2. Aims of the project
3. Material and methods
4. Work done and first results
5. Perspectives
6. Courses and seminars
25.09.2019
Water availability in 2040
INTERNATIONAL ORGANISATION OF VINE AND WINE (OIV)
WORLD RESOURCES INSTITUTEModels SSP2 and RCP8,5
INTRODUCTION
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION
Area under vines
Water availability in 2040
INTERNATIONAL ORGANISATION OF VINE AND WINE (OIV)
WORLD RESOURCES INSTITUTEModels SSP2 and RCP8,5
INTRODUCTION
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION
Area under vinesStrategies:
(Quenol et al., 2016)
Effects of rootstocks on water stress tolerance
INTRODUCTION
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION
ROOT DEPTH AND SIZE
TRASPIRATION BY LEAVES
VESSEL SIZE
STARCH STORAGE
Vitis species
Variability of rootstocks
INTRODUCTION
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION
V. riparia V. rupestris V. berlandieri
V. riparia × V. rupestris V. berlandieri× V. riparia V. Berlandieri× V. rupestris
• 3309 C
• 101-14
• Kober 5BB
• S.O.4
• 420 A
• 161.49
• 1103 Paulsen
• 140 Ruggeri
• 110 Richter
WA
TER
STR
ESS T
OLE
RA
NC
E
Padgett-Johnson et al., 2003
90% of Vitis vinifera grown in the world (Keller, 2015)
Variability of rootstocks
INTRODUCTION
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION
500Total
232Unique
70
Migliaro et al., 2019 Bianchi et al., 2018
30
Riccagioia Collection G series
CoreCollection
University of Milano
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
AIMS OF THE PROJECT
2. AIMS OF THE PROJECT
NEW DROUGHT-TOLERANT ROOTSTOCKS
DIFFERENT PHENOTYPING PARAMETERS
QTLs RELATED TO DROUGHT
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
EXPERIMENTAL DESIGN
3. MATERIAL AND METHODS
G seriesNew American
progeniesF1 population
Vitis hybrids Vitis speciesM1×V. berlandieri
NEW DROUGHT-TOLERANT ROOTSTOCKS
GENETIC VARIABILITY BY SNPs
30 200150
Core Collection
Vitis hybrids
70
PHENOTYPING FOR WATER STRESS
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
EXPERIMENTAL FIELDS
3. MATERIAL AND METHODS
ARCAGNA (LO)
New American progenies
G series
RICCAGIOIA (PV)
G series
F1 population
Core Collection
• Thermal indexes:
❖ Crop Water Stress Index (CWSI)
❖ Stomatal conductance index (IG)
Parameters
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
PHENOTYPING FOR WATER STRESS
3. MATERIAL AND METHODS
𝑪𝑾𝑺𝑰 =𝑻𝒍𝒆𝒂𝒇 − 𝑻𝒘𝒆𝒕
𝑻𝒅𝒓𝒚 − 𝑻𝒘𝒆𝒕
• Photosintesis (A);
• Stomatal conductance (gs);
• Traspiration (E);
• Intrinsic Water Use Efficency (iWUE);
𝑰𝑮 =𝑻𝒅𝒓𝒚 − 𝑻𝒍𝒆𝒂𝒇
𝑻𝒍𝒆𝒂𝒇 − 𝑻𝒘𝒆𝒕
𝒊𝑾𝑼𝑬 =𝑨
𝒈𝒔
SNP chip array
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
Genetic variability
3. MATERIAL AND METHODS
SPECIES SNPs
V. vinifera 13.6K
V. aestivalis
4.5K(33%)
V. berlandieri
V. labrusca
V. cinerea
V. lincecumï
M. rotundifolia
total 18K
Laucou et al., 2018
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
WORK DONE
4. WORK DONE AND FIRST RESULTS
G seriesNew American
progeniesF1 population
Vitis hybrids Vitis speciesM1×V. berlandieri
NEW DROUGHT-TOLERANT ROOTSTOCKS
GENETIC VARIABILITY BY SNPs
30 200150
Core Collection
Vitis hybrids
70
PHENOTYPING FOR WATER STRESS
water stress New experimental field
collection of leaves for
mineral analysis
DNA extraction from
100 genotypes
DNA extraction
FIRST RESULTS
Phenotyping of the G series
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 4. WORK DONE AND FIRST RESULTS
Parameter Source Deviance df Variance F α
Photosynthesis
SITE 2260.125 1 2260.125 504.847 0.00
GENOTYPE 821.309 30 27.377 6.115 0.00
Interaction 752.958 30 25.099 5.606 0.00
Error 577.514 129 4.477
Stomatal conductance
SITE 1085600 1 1085600 445.177 0.00
GENOTYPE 330339.6 30 11011.32 4.515 0.00
Interaction 451459 30 15048.63 6.171 0.00
Error 314577.2 129 2438.583
Water Use Efficiency (WUE)
SITE 65.947 1 65.947 18.213 0.00
GENOTYPE 469.033 30 15.634 4.318 0.00
Interaction 508.763 30 16.959 4.684 0.00
Error 467.094 129 3.621
G.03G.05
G.07
G.09G.12
G.13
G.16
G.17
G.19
G.21
G.23
G.24
G.25
G.26
G.27
G.28
G.30G.30 B
G.34
G.37
G.69
G.70
G.71
G.73
G.75
G.76
G.77
G.81G.82
G.83
M2
2
3
4
5
6
7
8
9
10
11
12
50 70 90 110 130 150 170 190 210 230
FIRST RESULTS
Phenotyping of the G series
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 4. WORK DONE AND FIRST RESULTS
Sampling time
CW
SI
So
il w
ate
r p
ote
ntia
l
(cb
ar)
AR
AR
RG
RG
Stomatal conductance (mmol m-2 s-1)
Ph
oto
sin
tesi
s(u
mo
lm-2
s-1)
FIRST RESULTS
Phenotyping of the G series by thermografy
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 4. WORK DONE AND FIRST RESULTS
R² = 0,6577
0
0,2
0,4
0,6
0,8
1
0 100 200 300 400
R² = 0,3386
0
4
8
12
16
20
0 100 200 300 400
CWSIIG
Stomatal conductance (mmol m-2 s-1) Stomatal conductance (mmol m-2 s-1)
Thermal indexes
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia
PERSPECTIVES
5. PERSPECTIVES
G seriesNew American
progeniesF1 population
Vitis hybrids Vitis speciesM1×V. berlandieri
NEW DROUGHT-TOLERANT ROOTSTOCKS
GENETIC VARIABILITY BY SNPs
30 200150
Core Collection
Vitis hybrids
70
PHENOTYPING FOR WATER STRESS
water stress
mineralsDNA extraction
limestone
water stressDNA extraction
water stress
2019
COURSES AND SEMINARS
Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 6. COURSES AND SEMINARS
Courses (University of Milan)
• Introduction to statistical analysis of ecological and environmental data
• Advanced statistical analysis of ecological and environmental data
• Senescence and quality of flowers and vegetables
• Exploitation of variability in woody crops
Seminars
• Riconoscimento delle principali specie da frutto e innovazione varietale
• Light and photosystems: the combination of growth
• Dal Medieval Warm Period al Current Warm Period: il clima europeo e la
circolazione atmosferica e oceanica