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DNA Technology . How is DNA fingerprinting used to identify a person? What is the use of polymerase chain reactions (PCR)? How is bacterial transformation used in medicine? How is gel electrophoresis used in DNA technology?. Vocabulary. Polymerase Chain Reaction Gel Electrophoresis - PowerPoint PPT Presentation
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DNA Technology • How is DNA fingerprinting used to identify a person?
• What is the use of polymerase chain reactions (PCR)?
• How is bacterial transformation used in medicine?
• How is gel electrophoresis used in DNA technology?
Vocabulary
• Polymerase Chain Reaction • Gel Electrophoresis • Bacterial Transformation • DNA Fingerprinting
Polymerase Chain Reaction
• Used to make many copies of small pieces of DNA
What is the use of polymerase chain reactions (PCR)?
DNA Fingerprinting
• Uses gel electrophoresis to observe the patterns that are unique to each person
How is DNA fingerprinting used to identify a person?
Gel Electrophoresis • a technique used to separate the DNA
fragments according to their size
How is gel electrophoresis used in DNA technology?
Bacterial Transformation • Bacteria can pick up DNA from their
environment and incorporate it into their DNA
How is bacterial transformation used in medicine?
BiotechnologyProvides:• Improved food products• Medical advances• An enhanced environment
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Biotechnology Breakthroughs• Insulin (1982)
– First commercial biotech product– Reliable, inexpensive source of
insulin• Rice
– Enriched with beta-carotene and iron• Bananas
– Containing edible hepatitis vaccine
Biotechnology Breakthroughs• Potatoes with higher solid content• Garlic that lowers cholesterol• Fruits and vegetables that reduce
risks of cancer and heart disease
Environmental Benefits
• Reduced pesticide use• Lower energy requirements• Cleaner water• Less soil erosion
Genetic Engineering and Bioethics • How has genetic engineering influenced food and
medicine?
• How is cloning currently used in food and medicine?
• How is stem cell research being used to benefit humans?
• What are the bioethics issues surrounding stem cell
research and cloning?
• What is the relationship of evolution to biological
resistance?
Vocabulary
• Cloning • Transgenic animals • Stem Cells • Bioethics • Biological Resistance
Cloning
How is cloning currently used in food and medicine?
• A body cell from one organism and an egg cell from another are fusedThe resulting cell divides like a normal embryo
•Making an identical copy of an organism
Transgenic animals • made by introducing genes from one species
into the genome of another organism.
Transgenic animals may be created to exploit the attributes of new genes (for example, genes for faster growth or larger muscles).
Agricultural scientists have engineered a number of crop plants with genes for desirable traits.
Stem Cells
• Is an unspecialized cell that can reproduce itself indefinitely and differentiate into specialized cells
How is stem cell research being used to benefit humans?
Biological Resistance
• Genetically based resistance to pests, disease, drought, etc.
What is the relationship of evolution to biological
resistance?
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Herbicide Resistant Crops
• Soybeans: Roundup Ready• Corn: Roundup Ready, Liberty Link • Cotton: BXN, Roundup Ready • Canola: Liberty Link, Roundup Ready
+ CP4 EPSPS = Roundup gene Ready
Bioethics
• study of the controversies brought about by advances in biology
What are the bioethics issues surrounding stem cell research and cloning?
How has genetic engineering influenced food and medicine?
Chromosomes and Mutations and Biological Resistance
• How do mutations affect evolution?
• What are genetic diseases that are caused by
mutations?
• What are the different types of mutations that can
occur in organisms?
• What is the relationship of evolution to biological
resistance?
Vocabulary• Single gene mutations • Polygenic mutations • Chromosomal mutations • Mitochondrial DNA mutations • nondisjunction • insertion • substitution • deletion • chromosomal translocation • mutagenic factors (radiation, chemical) • biological resistance • point mutation • frame shift mutation
Gene Mutations• Change in genes caused by change in
structure of the DNA • DNA bases may be substituted, added, or
removed to cause gene mutation • When genes are added or removed, the
mutation is called a frame shift mutation • Adding or Removing a genes is called a point
mutation
point mutation VS. frameshift mutations • A point mutation is a simple change in one base
of the gene sequence.– Original The fat cat ate the wee rat.– Point Mutation The fat hat ate the wee rat.
• In a frame shift mutation, one or more bases are inserted or deleted.– Original The fat cat ate the wee rat.– Frame Shift The fat caa tet hew eer at
mutations
Single gene and Polygenic •A permanent change in DNA
•Single gene mutation deals with 1 gene and polygenic mutations deal with more than 1 gene
Chromosomal mutations• Mutations in a cells’ chromosome
Mitochondrial DNA mutations• Mutations in the chromosome of
mitochondrial DNA
nondisjunction
• Sister chromatids do not separate properly during meiosis
• Results in gametes that do not have the correct number of chromosomes
•Inversion occurs when a piece of a chromosome breaks off & reattaches to the same place but in the reverse order
Inversion
(Mutations) insertion
• Results in the addition of extra DNA • Insertions can cause frameshift mutations, and general result in a nonfunctional protein.
– Original The fat cat ate the wee rat.– Insertion The fat cat xlw ate the wee rat.
(Mutations) Substitution
• Mutation results from genes or single nitrogen bases being switched.
•Duplications occur when a section of a chromosome is doubled
(Mutations) Duplication
(Mutations) deletion• Mutations that result from sections of DNA being deleted. • Deletions occur when the end of a chromosome breaks
off & is lost• These can be small, such as the removal of just one "word," or longer deletions that affect a large number of genes on the chromosome.
• Deletions can also cause frameshift mutations. In this example, the deletion eliminated the word cat.
– Original The fat cat ate the wee rat.– Deletion The fat ate the wee rat.
chromosomal translocation
• Translocation occurs when a chromosome segment breaks off & attaches to a different chromosome
• Parts of a chromosome braking off and reattaching during crossing over in meiosis.
mutagenic factors
• chemical or physical agents that change DNA to cause mutations.
• (radiation, chemical)
1. What are the different types of mutations that can occur in organisms?
2. What are genetic diseases that are caused by mutations?
3. How do mutations affect evolution?
What is the relationship of evolution to biological resistance?