Upload
judith-thomas
View
213
Download
0
Embed Size (px)
Citation preview
Dimensional AnalysisDimensional AnalysisUnits of measures commonly Units of measures commonly
encountered in the labencountered in the lab
Dimensional AnalysisDimensional AnalysisUnits of measures commonly Units of measures commonly
encountered in the labencountered in the lab
gmMng/l
femtogram
From Chapter 13: Seidman and From Chapter 13: Seidman and MooreMoore
I.A. ExponentsI.A. Exponents I.B. Scientific NotationI.B. Scientific Notation II.A. Common logarithimsII.A. Common logarithims II.B. AntilogarithimsII.B. Antilogarithims II.C. Natural logarithims – skip for nowII.C. Natural logarithims – skip for now II. D. pHII. D. pH III. UnitsIII. Units IV.A. EquationsIV.A. Equations IV.B. Units and Mathematical OperationsIV.B. Units and Mathematical Operations
Most Commonly Encountered UnitsMost Commonly Encountered Units
m (meter) g (gram) l (liter)
PrefixesPrefixesm (meter) g (gram) l (liter)
103 mm 103 mg 103 ml
106 m 106 g 106 l
109 nm 109 ng
1012 pg
1015 pg
Use prefixes in tables and graphs
Most Commonly Encountered UnitsMost Commonly Encountered Units
m (meter) g (gram) l (liter)
103 mm 103 mg 103 ml
106 m 106 g 106 l
109 nm 109 ng
1012 pg
1015 fg
Mass of protein, cells Volume of solutions
Weight of single cell
Wavelength of light Size of virus
Mass of DNA
Mass of DNA Volume of DNA or Enzyme
Size of cells
Derived Units - ConcentrationDerived Units - Concentration
g/l = mg/ml = g/l = ng/nl
Derived Units - ConcentrationDerived Units - Concentration
g/l = mg/ml = g/l = ng/nl
103 mg/l = 103 g/ml = 103 ng/l
Derived Units - ConcentrationDerived Units - Concentration
g/l = mg/ml = g/l = ng/nl
103 mg/l = 103 g/ml = 103 ng/l [DNA]
[DNA][Pro]
ppm
Derived Units - ConcentrationDerived Units - Concentration
g/l g/mol
=mol/l(M)
Molar ConcentrationsMolar Concentrations
g/l = mg/ml = g/l = ng/nl___ g/mol mg/mmol g/mol ng/nmol
=? =? =? =?
Molar ConcentrationsMolar Concentrations
g/l = mg/ml = g/l = ng/nl g/mol mg/mmol g/mol ng/nmol
= mol/l (moles per liter) = M (molar concentration)
mMmM
millimolar – units mmol/l – symbol mM
M x 103 eg. 0.05 M = 50 mM
Concentration of buffers (Tris, Phosphate) and other salts (EDTA) in solutions
MM
micromolar – units mol/l – symbol M
mM x 103 eg. 0.5 mM = 500 M
Concentration of trace minerals and vitamins
KM of many enzymes