Detection of glycerides in biodiesel 1 Team Members: Ben Wilson Matt Yourek Megan Templeton Mentor:...
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Enzyme Biosensor Detection of glycerides in biodiesel 1 Team Members: Ben Wilson Matt Yourek Megan Templeton Mentor: Dr. Tom Hess Client: Dr. Dev Shrestha
Detection of glycerides in biodiesel 1 Team Members: Ben Wilson Matt Yourek Megan Templeton Mentor: Dr. Tom HessClient: Dr. Dev Shrestha
Detection of glycerides in biodiesel 1 Team Members: Ben Wilson
Matt Yourek Megan Templeton Mentor: Dr. Tom HessClient: Dr. Dev
Shrestha
Slide 2
Design, fabricate, and test a handheld biosensor that uses a
modified pH meter combined with an immobilized enzyme electrode to
detect the amount of free and total glycerol in a sample of
biodiesel. 2
Slide 3
Currently biodiesel makers use a gas chromatograph to measure
the amount of glycerol within their biodiesel. While the GC
provides a very accurate measurement, it is a time consuming
process to prepare and test a sample. 3
Slide 4
Blood glucose meters Blood pressure sensors i-Spec Q-100 - uses
impedance spectroscopy technology, which is similar to infrared
spectroscopy 4
Slide 5
Small Light Fast response time Simple to use Accurate Easy to
calibrate Reusable Strong/Durable Inexpensive Sensitive to small
changes in pH User friendly 5
Slide 6
Dimensions 8x 6x 5 Weight of about 2 lbs Response time of 10
minutes or less Able to test four samples in one hour Error margin
of 5% of concentration measurement Can withstand repeated drops
from 5ft Does not bend or flex Storage for electrode Result is
automatically displayed in large numbers Total of five buttons or
less Visual or audio signal when measurement is complete Audio
signal on button push Large buttons that take < 3.5lbf to push
Matte/textured finish for added grip Battery operated Takes user
< 5 minutes to change electrode Automatic zeroing upon button
push Visual or audio signal for low battery One electrode can be
used up to 50 times Costs less than $300 to fabricate 6
Slide 7
Pros: Very similar to pH meter Easy to store membrane Cons:
Higher chance of damage to electrode Could experience bad
connection between electrode and instrument 7
Slide 8
8
Slide 9
9
Slide 10
Pros: Simple to use Doesnt need to be held 10 Cons: Would need
to be more durable/sealed More complicated to wash and store
membrane
Slide 11
Gelatin-Lipase membrane - cited in literature research paper
PVA membrane - found during membrane research PVDF membrane - found
during membrane research 11
Slide 12
Gelatin-Lipase Membrane Pros: Easy to make Similar testing
published in papers Cons: Published paper does not include all
information Membrane falls apart in storage buffer and water Does
not give reliable mV readings 12
Slide 13
PVA Membrane Pros: Mechanically strong and flexible Resistant
to oil and solvent Has ionic conductivity that allows easy flow of
electrons Used in a biosensor to detect glycerides Cons: Complex
process to create membrane Water-soluble, so it might dissolve
during reaction 13
Slide 14
PVDF Membrane Pros: Resistant to acids, bases, and solvents
Mechanically strong Low cost Cons: Requires very advanced
equipment/techniques A detailed process to create Used for the
growth of neurons, not typically used with a biosensor 14
Slide 15
Tested both pure canola oil and a biodiesel sample using the
gelatin-lipase membrane The following graphs are the results of
millivolt change during the testing process 15
Slide 16
Pure canola oil sample 16 Peak to peak average = 22 mV
Slide 17
Biodiesel 30 minute sample (Part 1) 17 Peak to peak average =
18 mV
Slide 18
Biodiesel 30 minute sample (Part 2) 18 Peak to peak average =
18 mV
Slide 19
Using the estimated peak to peak value for each test, as well
as the glyceride percent concentration we can obtain the following
two point calibration 19
Slide 20
Arduino UNO with ATmega328 Pros: Easy to use/program Comes in
design kits Lots of memory LCD screen comes with wiring
instructions for Arduino Cons: New programming language to learn
Minimum voltage input of 6V Slower clock rate Problems could be
made worse by mass of online information 20
Slide 21
PICAXE 14M2 Pros: Familiar programming language Faster clock
rate Dr. Wolbrecht teaches a class that uses them Cons:
Manufactured and distributed from UK Not much programming
help/information Less memory 21
Slide 22
Instrument - Design 1, external electrode design Membrane - N/A
Microcontroller - Option 1, Arduino UNO with ATmega328 LCD screen -
hacktronics LCD screen, comes with Arduino wiring Housing material
- ABS 3D printed plastic 22
Slide 23
Creation and testing of PVA and PVDF membranes Effect of
multiple layers of cellulose acetate solution on reaction time and
membrane preservation Relation of mV amplitude to concentration
percent 23
Slide 24
Nov 30 Dec 9: Testing of gelatin-lipase membrane Dec 2:
Snapshot Dec 6: Design review Christmas Break Jan 17 Jan 25:
Testing of PVA membrane Jan 26 Feb 7: Testing of PVDF membrane Feb
8 Feb 24: Finishing touches on design and ordering of any parts Feb
27 Mar 9: Fabrication Spring Break Mar 19 Apr 26: Validation Apr
27: Engineering Expo 24