Dendritic Cell Research Tools, Product Flyer

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Dendritic Cell Research Tools, Product Flyer

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R E S E A R C H T O O L S

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R E S E A R C H T O O L SDendritic Cell

Dendrit ic Cells

NF- B Pathways

Inflammation

Cytokines & Chemokines

Screening Servicesfor Inflammation & Immune Signaling Pathways

Inflammation/Immune Signaling Pathway

Dendritic Cells 1Application Key FA-A - Functional Assay (Activation) FA-Neut - Functional Assay (Neutralization) Flow-CS+Intra - Flow (Cell Surface) and Flow (Intracellular) IF/ICC - Immunofluorescence/Immunocytochemistry CM - Confocal Microscopy Flow-CS - Flow (Cell Surface) Flow-Intra - Flow (Intracellular) IHC-Fr - Immunohistochemistry (Frozen) IP - Immunoprecipitation IHC-P - Immunohistochemistry (Paraffin) WB - Western Blot PAP Peptide Affinity Purified

Dendritic Cell antibodiesThe fi rst dendritic cells (DCs) discovered, in 1868, were the Langerhans cells of human epidermis. However, it took until the 1970’s to demonstrate that these cells belonged to the im-

mune system. In 1973, the pioneering work of Steinman and Cohn permitted the identifi cation of DCs in lymphoid tissue and their functional relationship with Langerhans cells. Realization of the ex-traordinary capacity of DCs for antigen-presentation set the stage for an exponentially rising interest in their biology. Major advances in the early 1990s subsequently led to the ability to generate DCs in vitro from myeloid hematopoietic progenitors or from monocytes, and greatly facilitated their study. The initial unifi ed model of DC life history held that immature DCs patrol peripheral tissues and upon encounter with microbial products or other danger signals undergo maturation as they migrate to lymphoid tissue where they present antigen and activate naive T cells.

While most elements of this model still hold true, in particular the unique capacity of DCs to initiate adaptive immunity, many different and contrasting facets of DCs have since been discovered. One aspect that has become clearly appreciated is the great diversity of DC subtypes with considerable functional differences. Part of this heterogeneity is intrinsic (eg “conventional” versus plasmacytoid DCs), but a high degree of plasticity is also characteristic of the DC system. For instance, DCs can be instructed by the nature of the early signals they receive, with greatly divergent consequences on the immune response. Thus, in addition to their clas-sic function to drive strong Th1-type adaptive responses, DCs can be polarized by microbial products towards a Th2- type response, or towards peripheral immune tolerance via the induction of regulatory T cells.

Today, DCs are positioned as the master regulators of immunity. Pharmacological intervention to exploit the full range of DC regulatory potential will undoubtedly lead to a variety of therapeutic applications to either boost, suppress or repolar-ize the immune system. Another recently recognized important function of DCs is to link the innate and adaptive immune response. This is illustrated by antiviral responses of plasmacytoid DCs, and by crosstalk between DCs and Natural Killer cells. A major breakthrough in DC biology has been the recent unraveling of the mechanisms responsible for their regulatory functions, an advance made possible by the molecular cloning of genes expressed by DCs. Thus, it was realized that DCs are remarkably equipped with Pattern Recognition Receptors (PRRs), the in-

Dendritics

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antibodies Dendritic Cell

nate sensors that recognize conserved molecular patterns on microbes and self- tissue. Major PRRs include the C-type Lectin Receptors and the Toll-Like Receptors. The key role played by Chemokines and their recep-tors in the migration patterns of DCs is now well established. Finally, an array of Cytokines and corresponding receptors are known to be responsible for the crosstalk between DCs and a host of other cell types that will determine the net outcome of the immune response. Collectively, this rapidly-evolving knowledge allows for drug-discovery programs to design pharmacological compounds to agonize or antagonize DC molecules in a number of clinical settings.

IMGENEX is pleased to announce that it has signed an exclusive distribution agreement with DENDRITICS SAS (Dardilly, France) to make their portfolio of highly characterized and validated monoclonal Dendritic cell research antibodies available to researchers in North America and India. Founded in 2005, the goal of DEN-DRITICS SAS was to make their technology and expertise available to the commercial market where other researchers could benefi t from these innovative reagents. DENDRITICS’ reagents have been validated and reported in many peer reviewed publications and can be used in fl ow cytometric analysis, IHC, cell depletion and blocking assays. All antibodies are purifi ed and most are available in a multiple formats.

DENDRITICS SAS research reagents are categorized into six major research focus areas: Dendritic Cell (DC) subtypes, C-type Lectin Receptors (CLRs), Toll-like Receptors (TLRs), DC Migration, Cytokines and Cytokine Receptors, Antigen processing & Presentation/Allergy and include IL7R, pDC/IPC clone 120G8, DCIR, DC-LAMP clone 1010E1, DC-LAMP clone 104G4 and Mouse Langerin.

About DENDRITICSDENDRITICS was formed in 2005 by former members of SCHERING PLOUGH Laboratory for Immunological Research (LIR) in Dardilly, France. The initial drive for DENDRITICS was inspired by it’s desire to supply the scientifi c community with the large collection of monoclonal antibodies developed at LIR. Most of the antibodies available directly result from participation in gene discovery programs and functional studies. Consequently, we strongly believe that DENDRITICS has a competitive advantage that ensures a high quality product and offers an expert understanding of the underlying science.

From their long experience at LIR, the team members of DENDRITICS have gathered complementary scientifi c and technological knowledge from different areas of immunology. The initial focus of LIR was the biology of B lymphocytes, elaboration of human monoclonal antibodies, and in cytokine discovery (IL- 3, IL- 4, IL- 7, IL- 10, IL- 17, GMCSF) together with their colleagues at DNAX Research Institute in Palo Alto, California. Interest in cytokines led to the fi nding that hu-man dendritic cells (DCs) could be generated in vitro from CD34+ hematopoietic progenitors cultured in the presence of GMCSF and TNFa. The period from 1992 to 2005 saw the specialization of LIR in DC biology and was fueled by a large-scale effort to discover and study the function of genes specifi cally expressed by these cells. This program led to a number of fi ndings that contributed in particular to understanding the heterogeneity of DCs, the pathways regulating DC migration, the function of C-type lectin receptors in DCs, and the activity of Toll-like receptors in DCs. Many novel genes were discovered through screening of cDNA libraries or through monoclonal antibodies raised against DCs. This collective effort and the motivation of DENDRITICS team members to stay at the forefront of science makes it possible to supply an extensive range of monoclonal antibodies for dendritic cell research. DENDRITICS is com-mited to the vision of providing innovative reagents to meet the upcoming needs of immunologists and will leverage its long-standing collaborations with the clinical community to drive the development of these new tools.


Dendritic Cell antibodies

Description Cat No Format Clone Host Species Application

Basophil DDX0030 Purifi ed 212H6.16 Mouse H Flow-CS, IF/ICC

DDX0030A488 Alexa Fluor® 488 212H6.16 Mouse H Flow-CS, IF/ICC

DDX0030A546 Alexa Fluor® 546 212H6.16 Mouse H IF/ICC

DDX0030A647 Alexa Fluor® 647 212H6.16 Mouse H Flow-CS, IF/ICC

CD127/IL-7R DDX0700 Purifi ed R34-34 Mouse H FA-Neut, Flow-CS, IP

DDX0700A488 Alexa Fluor® 488 R34-34 Mouse H Flow-CS

DDX0700A546 Alexa Fluor® 546 R34-34 Mouse H IHC-P

DDX0700A647 Alexa Fluor® 647 R34-34 Mouse H Flow-CS, IHC-P

CD1a DDX0080 Purifi ed 214A9.01 Mouse H Flow-CS, IHC-Fr

DDX0080A488 Alexa Fluor® 488 214A9.01 Mouse H Flow-CS, IHC-Fr

DDX0080A546 Alexa Fluor® 546 214A9.01 Mouse H IHC-Fr


DDX0080B Biotin 214A9.01 Mouse H Flow-CS, IHC-Fr

CD1a DDX0081 Purifi ed 201B5.08 Mouse H Flow-CS, IHC-Fr, WB

DDX0081A488 Alexa Fluor® 488 201B5.08 Mouse H Flow-CS, IHC-Fr



CD40 DDX0130 Purifi ed G28-5 Mouse H FA-A, Flow-CS

CD40 DDX0131 Purifi ed mAb89 Mouse H FA-A, Flow-CS, IHC-P

DDX0131A488 Alexa Fluor® 488 mAb89 Mouse H Flow-CS

CD80/B7-1 DDX0160 Purifi ed mAb104 Mouse H Flow-CS

CD205 (DEC-205) DDX0020 Purifi ed NLDC-145 Rat M Flow-CS, IHC-P

DDX0020A488 Alexa Fluor® 488 NLDC-145 Rat M Flow-CS, IHC-P

DDX0020B Biotin NLDC-145 Rat M Flow-CS, IHC-P

CD207/Langerin DDX0360 Purifi ed 808E10.01 Mouse H, M, R FA-Neut, Flow-CS, IHC-Fr

DDX0360A488 Alexa Fluor® 488 808E10.01 Mouse H, M, R Flow-CS, IHC-Fr

DDX0360A546 Alexa Fluor® 546 808E10.01 Mouse H, M, R IHC-Fr

DDX0360B Biotin 808E10.01 Mouse H, M, R Flow-CS, IHC-Fr

CD207/Langerin DDX0361 Purifi ed 310F7.02/HD26 Mouse H, M, R Flow-Intra, IHC-P, WB

DDX0361A488 Alexa Fluor® 488 310F7.02/HD26 Mouse H, M, R Flow-Intra, IHC-P

DDX0361A546 Alexa Fluor® 546 310F7.02/HD26 Mouse H, M, R IHC-P

DDX0361A647 Alexa Fluor® 647 310F7.02/HD26 Mouse H, M, R Flow-Intra, IHC-P

DDX0361APC APC 310F7.02/HD26 Mouse H, M, R Flow-Intra, IHC-P

DDX0361B Biotin 310F7.02/HD26 Mouse H, M, R IHC-P, WB

DDX0361PE PE 310F7.02/HD26 Mouse H, M, R Flow-Intra, IHC-P

CD207/Langerin DDX0362 Purifi ed 929F3.01 Rat H, M, R Flow-Intra, IF/ICC, IHC-Fr

DDX0362A488 Alexa Fluor® 488 929F3.01 Rat H, M, R Flow-Intra, IF/ICC, IHC-Fr

DDX0362A546 Alexa Fluor® 546 929F3.01 Rat H, M, R IF/ICC, IHC-Fr, IHC-P

DDX0362A647 Alexa Fluor® 647 929F3.01 Rat H, M, R Flow-Intra, IF/ICC, IHC-Fr

DDX0362B Biotin 929F3.01 Rat H, M, R IF/ICC, IHC-Fr, IHC-P

CD207/Langerin DDX0363 Purifi ed DCGM4/122D5.03 Mouse H Flow-CS, IHC-P, WB

DDX0363A488 Alexa Fluor® 488 DCGM4/122D5.03 Mouse H Flow-CS, IHC-P

DDX0363A546 Alexa Fluor® 546 DCGM4/122D5.03 Mouse H IHC-P

DDX0363A647 Alexa Fluor® 647 DCGM4/122D5.03 Mouse H Flow-CS, IHC-P

DDX0363B Biotin DCGM4/122D5.03 Mouse H Flow-CS, IHC-P, WBImmunofl uorescence on mouse epidermal LC CD11c+ using 929F3.01.

CD207/Langerin (DDX0362)



CD207/Langerin DDX0368 Purifi ed 306G9 Mouse H, M Flow-Intra, IF/ICC, IHC-P

DDX0368A488 Alexa Fluor® 488 306G9 Mouse H, M Flow-Intra, IHC-P

DDX0368A546 Alexa Fluor® 546 306G9 Mouse H, M IF/ICC, IHC-P

CD207/Langerin DDX0370 Purifi ed 205C1 Mouse M Flow-Intra, WB

DDX0370A488 Alexa Fluor® 488 205C1 Mouse M Flow-Intra

DDX0370A546 Alexa Fluor® 546 205C1 Mouse M IHC-P

DDX0370A647 Alexa Fluor® 647 205C1 Mouse M Flow-Intra, IHC-P

DDX0370B Biotin 205C1 Mouse M Flow-Intra, WB

CD207/Langerin DDX0373 Purifi ed 923B7 Rat H, M, R Flow-CS, IHC-Fr

DDX0373A488 Alexa Fluor® 488 923B7 Rat H, M, R Flow-CS, IHC-Fr

DDX0373A546 Alexa Fluor® 546 923B7 Rat H, M, R IHC-Fr

CD208/DC-LAMP DDX0190 Purifi ed 104G4 Mouse H Flow-Intra, IHC-Fr, IHC-P, WB

DDX0190A488 Alexa Fluor® 488 104G4 Mouse H Flow-Intra, IHC-Fr, IHC-P

DDX0190A546 Alexa Fluor® 546 104G4 Mouse H IHC-Fr, IHC-P

DDX0190B Biotin 104G4 Mouse H Flow-Intra, IHC-Fr, IHC-P, WB

CD208/DC-LAMP DDX0191 Purifi ed 1010E1.01 Rat Multi-species IHC-Fr, IHC-P

DDX0191A488 Alexa Fluor® 488 1010E1.01 Rat Multi-species IHC-Fr, IHC-P

DDX0191A546 Alexa Fluor® 546 1010E1.01 Rat Multi-species IHC-Fr, IHC-P

DDX0191B Biotin 1010E1.01 Rat Multi-species IHC-Fr, IHC-P, WB

CD208/DC-LAMP DDX0192 Purifi ed 1006F7.01 Rat M IHC-Fr

DDX0192A488 Alexa Fluor® 488 1006F7.01 Rat M Flow-CS

DDX0192A647 Alexa Fluor® 647 1006F7.01 Rat M Flow-CS

CD208/DC-LAMP DDX0193 Purifi ed 109G3 Mouse H Flow-Intra, IHC-Fr

DDX0193A488 Alexa Fluor® 488 109G3 Mouse H Flow-Intra, IHC-Fr

DDX0193A546 Alexa Fluor® 546 109G3 Mouse H IHC-Fr

DDX0193A647 Alexa Fluor® 647 109G3 Mouse H IHC-Fr

DDX0193B Biotin 109G3 Mouse H Flow-Intra, IHC-Fr

CD21 isoform DDX0120 Purifi ed 7D6 Mouse H FA-A, IHC-Fr

CD301/ASGPR DDX0010 Purifi ed 125A10.03 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0010A488 Alexa Fluor® 488 125A10.03 Mouse H Flow-CS, Flow-Intra, IHC-Fr


DDX0010A647 Alexa Fluor® 647 125A10.03 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0010B Biotin 125A10.03 Mouse H Flow-CS, Flow-Intra, IHC-Fr

CD303/BDCA-2 DDX0040 Purifi ed 108H10.03 Mouse H Flow-CS, IHC-Fr, IHC-P

DDX0040A488 Alexa Fluor® 488 108H10.03 Mouse H Flow-CS, IHC-Fr, IHC-P

DDX0040A546 Alexa Fluor® 546 108H10.03 Mouse H IHC-Fr, IHC-P

DDX0040A647 Alexa Fluor® 647 108H10.03 Mouse H Flow-CS, IHC-Fr, IHC-P

DDX0040B Biotin 108H10.03 Mouse H Flow-CS, IHC-Fr, IHC-P

CD303/BDCA-2 DDX0041 Purifi ed 104C12.08 Mouse H Flow-CS, IHC-P

DDX0041A488 Alexa Fluor® 488 104C12.08 Mouse H Flow-CS, IHC-P

DDX0041A546 Alexa Fluor® 546 104C12.08 Mouse H IHC-P

DDX0041A647 Alexa Fluor® 647 104C12.08 Mouse H Flow-CS, IHC-P

DDX0041B Biotin 104C12.08 Mouse H Flow-CS, IHC-P

Paraffi n-embedded tonsil human pDCs probed with DDX0043.

CD303/BDCA-2 (DDX0043)





CD303/BDCA-2 DDX0042 Purifi ed 110H7.05 Mouse H FA-Neut, Flow-CS, IHC-P

DDX0042A488 Alexa Fluor® 488 110H7.05 Mouse H Flow-CS

DDX0042A546 Alexa Fluor® 546 110H7.05 Mouse H IF/ICC

DDX0042A647 Alexa Fluor® 647 110H7.05 Mouse H Flow-CS

DDX0042B Biotin 110H7.05 Mouse H FA-Neut, Flow-CS, IHC-P

CD303/BDCA-2 DDX0043 Purifi ed 124B3.13 Mouse H, M, R Flow-Intra, IHC-P

DDX0043A488 Alexa Fluor® 488 124B3.13 Mouse H, M, R Flow-Intra, IHC-P

DDX0043A546 Alexa Fluor® 546 124B3.13 Mouse H, M, R IHC-P

DDX0043A647 Alexa Fluor® 647 124B3.13 Mouse H, M, R Flow-Intra, IHC-P

DDX0043B Biotin 124B3.13 Mouse H, M, R Flow-Intra, IHC-P

DCIR DDX0180 Purifi ed 111F8.04 Mouse H Flow-CS, IHC-Fr

DDX0180A488 Alexa Fluor® 488 111F8.04 Mouse H Flow-CS, IHC-Fr

DDX0180A546 Alexa Fluor® 546 111F8.04 Mouse H IHC-Fr

DDX0180B Biotin 111F8.04 Mouse H Flow-CS, IHC-Fr

DDX0180FITC FITC 111F8.04 Mouse H Flow-CS, IHC-Fr

DDX0180PE PE 111F8.04 Mouse H Flow-CS, IHC-Fr

DC-SIGN DDX0202 Purifi ed 102E11.06 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0202A488 Alexa Fluor® 488 102E11.06 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0202A546 Alexa Fluor® 546 102E11.06 Mouse H IHC-Fr

DDX0202B Biotin 102E11.06 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DC-SIGN DDX0203 Purifi ed 103G2.07 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0203A488 Alexa Fluor® 488 103G2.07 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0203A546 Alexa Fluor® 546 103G2.07 Mouse H IHC-Fr

DDX0203A647 Alexa Fluor® 647 103G2.07 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0203B Biotin 103G2.07 Mouse H Flow-CS, Flow-Intra, IHC-Fr

Anti-CD303 (Clone 124B3.13, Cat. No. DDX0043): Plasmacytoid Dendritic Cell Marker

CD303 in Kikuchi Disease or Histiocytic Necrotizing Lymphadenitis can be mistaken for Lymphoma or SLE. Kikuchi disease usually follows a benign course1.

CD303 in Lymphoma Cutis. Lymphoma cutis is a type of non-Hodgkin’s lymphoma (NHL). Sometimes conditions can look similar to Lymphocytoma cutis which follows a benign course. A skin biopsy is needed to be sure of diagnosis2-3.

CD303 in breast tumor tissue.




DC-SIGN DDX0204 Purifi ed 108C7.01 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0204A488 Alexa Fluor® 488 108C7.01 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0204A546 Alexa Fluor® 546 108C7.01 Mouse H IHC-Fr

DDX0204A647 Alexa Fluor® 647 108C7.01 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0204B Biotin 108C7.01 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DC-SIGN DDX0205 Purifi ed 102F10.04 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0205A488 Alexa Fluor® 488 102F10.04 Mouse H Flow-CS, Flow-Intra, IHC-Fr

DDX0205A546 Alexa Fluor® 546 102F10.04 Mouse H IHC-Fr

DC-SIGN-like DDX0200 Purifi ed 118A8.05 Mouse H Flow-CS, IF/ICC, IHC

DDX0200A488 Alexa Fluor® 488 118A8.05 Mouse H Flow-CS, IF/ICC, IHC

DDX0200A546 Alexa Fluor® 546 118A8.05 Mouse H IF/ICC, IHC

DDX0200A647 Alexa Fluor® 647 118A8.05 Mouse H Flow-CS, IF/ICC, IHC

DDX0200B Biotin 118A8.05 Mouse H Flow-CS

DORA/IgSF6 DDX0220 Purifi ed 104A10.01 Mouse H, M Flow-CS

DDX0220A488 Alexa Fluor® 488 104A10.01 Mouse H, M Flow-CS

DDX0220A546 Alexa Fluor® 546 104A10.01 Mouse H, M Flow-CS, IF/ICC

DDX0220A647 Alexa Fluor® 647 104A10.01 Mouse H, M Flow-CS

DDX0220B Biotin 104A10.01 Mouse H, M Flow-CS

FDF03 DDX0230 Purifi ed 36H2 Rat H Flow-CS, IP, WB

DDX0230A488 Alexa Fluor® 488 36H2 Rat H Flow-CS

DDX0230A546 Alexa Fluor® 546 36H2 Rat H IHC-P

DDX0230A647 Alexa Fluor® 647 36H2 Rat H Flow-CS

DDX0230B Biotin 36H2 Rat H Flow-CS, IP, WB

gIFN-R/CD119 DDX0001 Purifi ed A6C5 Mouse H FA-A, IF/ICC, IHC-P

HME-MMP12 DDX0280 Purifi ed 603E6.22 Mouse H IHC-Fr

DDX0280B Biotin 603E6.22 Mouse H IHC-Fr

HME-MMP12 DDX0281 Purifi ed 706F9.01 Mouse H Peptide ELISA*

DDX0281B Biotin 706F9.01 Mouse H Peptide ELISA*

DDX0281HRPO HRP 706F9.01 Mouse H Peptide ELISA*

HME-MMP12 DDX0282 Purifi ed 705D10.11 Mouse H ELISA, WB

HME-MMP12 DDX0284 Purifi ed 701E4.03 Mouse H Peptide ELISA*

IL3-Ra/CD123 DDX0300 Purifi ed 107D2.08 Mouse H Flow-CS, IHC-Fr, IHC-P, IP

DDX0300A488 Alexa Fluor® 488 107D2.08 Mouse H Flow-CS, IHC-Fr, IHC-P

DDX0300A546 Alexa Fluor® 546 107D2.08 Mouse H IHC-Fr, IHC-P

DDX0300A647 Alexa Fluor® 647 107D2.08 Mouse H Flow-CS, IHC-Fr, IHC-P

Infl uenza M1/ DDX0270 Purifi ed 405H1.01 Mouse H ELISA, Flow-Intra

DDX0270A488 Alexa Fluor® 488 405H1.01 Mouse H FA-A, Flow-Intra

DDX0270A647 Alexa Fluor® 647 405H1.01 Mouse H Flow-Intra

DDX0270B Biotin 405H1.01 Mouse H ELISA

IRAK-4 DDX0341 Purifi ed 7D8 Mouse H IF/ICC, WB

DDX0341A488 Alexa Fluor® 488 7D8 Mouse H IF/ICC

DDX0341A546 Alexa Fluor® 546 7D8 Mouse H IF/ICC

MIP-3a/CD196 DDX0420 Purifi ed 206D9.05 Mouse H FA-Neut, IHC-Fr, IHC-P, WB

DDX0420HRPO HRP 206D9.05 Mouse H ELISA

MIP-3a/CD196 DDX0430 Purifi ed 319F6.06 Mouse H FA-Neut, IHC-P

Flow Cytometry staining: Purifi ed 104A10.01+goat anti mouse FITC probing COP5 fi broblasts transiently transfected with human DORA cDNA.

DORA/IgSF6 (DDX0220)



CD19 & CD3epsilon depleted mouse spleen cells were gated for pDC cells (Fig. 1) and double stained using B220-FITC, and anti-pDC-APC (DDX0390APC) at 1ug/ml (Fig. 3). Appropriate isotype control shown in Fig. 2

pDC/IPC (DDX0390)

MIP-3a/CD196 DDX0431 Purifi ed 308B7.06 Mouse H IHC-P

DDX0431HRPO HRP 308B7.06 Mouse H IHC-P

Neuropilin-1 DDX0440 Purifi ed 211H6.01 Mouse H Flow-CS, IHC-Fr

DDX0440A488 Alexa Fluor® 488 211H6.01 Mouse H Flow-CS, IHC-Fr

DDX0440A647 Alexa Fluor® 647 211H6.01 Mouse H Flow-CS, IHC-Fr

pDC/IPC DDX0390 Purifi ed 120G8.04 Rat M Flow-CS, Flow-Intra, IHC-Fr,

in vivo depletion

DDX0390A488 Alexa Fluor® 488 120G8.04 Rat M Flow-CS, IHC-Fr, in vivo depletion

DDX0390A546 Alexa Fluor® 546 120G8.04 Rat M IHC-Fr

DDX0390A647 Alexa Fluor® 647 120G8.04 Rat M Flow-CS, IHC-Fr, in vivo depletion

DDX0390APC APC 120G8.04 Rat M Flow-CS, Flow-Intra, IHC-Fr

DDX0390B Biotin 120G8.04 Rat M Flow-CS, in vivo depletion

DDX0390FITC FITC 120G8.04 Rat M Flow-CS, Flow-Intra, IHC-Fr

DDX0390PE PE 120G8.04 Rat M Flow-CS, Flow-Intra, IHC-Fr

Sema-6A DDX0462 Purifi ed 104B3 Mouse H IHC-Fr

Sema-6A DDX0463 Purifi ed 118E7 Mouse H Flow-CS, IHC

DDX0463A488 Alexa Fluor® 488 118E7 Mouse H Flow-CS, IHC

DDX0463A647 Alexa Fluor® 647 118E7 Mouse H Flow-CS, IHC

TLR3/CD283 DDX0470 Purifi ed 619F7.06 Mouse H Flow-Intra, IF/ICC, IHC-Fr, IHC-P

DDX0470A488 Alexa Fluor® 488 619F7.06 Mouse H Flow-Intra, IF/ICC, IHC-Fr, IHC-P

DDX0470A546 Alexa Fluor® 546 619F7.06 Mouse H IF/ICC, IHC-P

DDX0470A647 Alexa Fluor® 647 619F7.06 Mouse H Flow-Intra, IF/ICC, IHC-Fr, IHC-P

DDX0470B Biotin 619F7.06 Mouse H Flow-Intra, IHC-Fr, WB

TLR3/CD283 DDX0474 Purifi ed 713E4.06 Mouse Multi-species Flow-Intra, IHC-P

DDX0474A488 Alexa Fluor® 488 713E4.06 Mouse Multi-species Flow-Intra, IHC-P



DDX0474B Biotin 713E4.06 Mouse Multi-species Flow-Intra, IHC-P

TLR3/CD283 DDX0475 Purifi ed 716G10.15 Mouse H, M, R Flow-Intra, IHC-Fr

DDX0475A488 Alexa Fluor® 488 716G10.15 Mouse H, M, R Flow-Intra, IHC-Fr

DDX0475A546 Alexa Fluor® 546 716G10.15 Mouse H, M, R IHC-Fr

DDX0475A647 Alexa Fluor® 647 716G10.15 Mouse H, M, R Flow-Intra, IHC-Fr

DDX0475B Biotin 716G10.15 Mouse H, M, R Flow-Intra, IHC-Fr

TLR8/CD288 DDX0480 Purifi ed 303F1.14 Mouse H Flow-Intra, IF/ICC, IHC-Fr, WB

DDX0480A488 Alexa Fluor® 488 303F1.14 Mouse H Flow-Intra, IF/ICC, IHC-Fr, WB

DDX0480A546 Alexa Fluor® 546 303F1.14 Mouse H IF/ICC, IHC-Fr

DDX0480A647 Alexa Fluor® 647 303F1.14 Mouse H Flow-Intra, IF/ICC, IHC-Fr, WB

DDX0480B Biotin 303F1.14 Mouse H Flow-Intra, IF/ICC, IHC-Fr, WB

TLR8/CD288 DDX0483 Purifi ed 112H7.15 Rat H Flow-Intra, IF/ICC, WB

DDX0483A488 Alexa Fluor® 488 112H7.15 Rat H Flow-Intra, IF/ICC, WB

DDX0483A546 Alexa Fluor® 546 112H7.15 Rat H IF/ICC

DDX0483A647 Alexa Fluor® 647 112H7.15 Rat H Flow-Intra, IF/ICC, WB

DDX0483B Biotin 112H7.15 Rat H Flow-Intra, IF/ICC, WB





CD Markers

CD1a IMG-80264 T.C. Sup. O10 Mouse H IHC-P

CD11b IMG-6292A Purifi ed 5C6 Rat M Flow-CS

IMG-6292C FITC 5C6 Rat M Flow-CS

IMG-6292D PE 5C6 Rat M Flow-CS

IMG-6292G APC 5C6 Rat M Flow-CS

CD11b IMG-6328A Purifi ed 44aacb Mouse H Flow-CS

IMG-6328AF647 Alexa Fluor® 647 44aacb Mouse H Flow-CS

IMG-6328C FITC 44aacb Mouse H Flow-CS

IMG-6328D PE 44aacb Mouse H Flow-CS

CD11c IMG-6293AF647 Alexa Fluor® 647 N418 Hamster M Flow-CS

IMG-6293C FITC N418 Hamster M Flow-CS

IMG-6293D PE N418 Hamster M Flow-CS

CD14 IMG-3991 PAP N/A Goat H WB

CD14 IMG-6350A Purifi ed RPA-M1 Mouse H Flow-CS

IMG-6350AF647 Alexa Fluor® 647 RPA-M1 Mouse H Flow-CS

IMG-6350C FITC RPA-M1 Mouse H Flow-CS

IMG-6350E Azide-Free RPA-M1 Mouse H Flow-CS

IMG-6350H PerCP-Cy5.5 RPA-M1 Mouse H Flow-CS

CD34 IMG-80073 Purifi ed QBEND/10 Mouse Multi-species IF/ICC, IHC-Fr, IHC-P, WB

CD34 IMG-6540C FITC ICO-115 Mouse H Flow-CS, IF/ICC

CD40 DDX0130 Purifi ed G28-5 Mouse H FA-A, Flow-CS

CD40 DDX0131A488 Alexa Fluor® 488 mAb89 Mouse H Flow-CS

CD40 IMG-5714 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB

CD47 IMG-6581E Azide Free B6H12.2 Mouse H FA-Neut, Flow-CS, IP, WB

CD61 IMG-80110 T.C. Sup. Y2/51 Mouse H IHC-P

CD80/B7-1 DDX0160 Purifi ed mAb104 Mouse H Flow-CS

CD86/B7-2 IMG-6582AF488 Alexa Fluor® 488 GL1 Rat M Flow-CS

IMG-6582AF647 Alexa Fluor® 647 GL1 Rat M Flow-CS

IMG-6582E Azide Free GL1 Rat M FA-Neut, Flow-CS, IF/ICC,

IHC-Fr, IP

CD274/B7-H1 IMG-3791 PAP N/A Goat H WB

CD281/TLR1 IMG-5012 Purifi ed N/A Rabbit H, M, R Flow-CS, Flow-Intra, WB

IMG-5012AF488 Alexa Fluor® 488 N/A Rabbit H, M, R Flow-CS+Intra

IMG-5012AF647 Alexa Fluor® 647 N/A Rabbit H, M, R Flow-CS+Intra

CD281/TLR1 IMG-5629 Purifi ed N/A Rabbit H, M WB

CD282/TLR2 IMG-319 Ascites 1030A5.138 Mouse H Flow-CS, Flow-Intra, IHC-P, WB

CD282/TLR2 IMG-410A Purifi ed N/A Rabbit H, M IHC-P, WB

IMG-410E Azide-Free N/A Rabbit H, M WB

CD282/TLR2 IMG-416A Purifi ed TL2.1 Mouse Dog, H Flow-CS+Intra, IF/ICC, IHC-P, IP,

WB

IMG-416AF488 Alexa Fluor® 488 TL2.1 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC

IMG-416B Biotin TL2.1 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC, IP

IMG-416C FITC TL2.1 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC

IMG-416D PE TL2.1 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC

IMG-416E Azide-Free TL2.1 Mouse Dog, H FA-Neut, Flow-CS, Flow-Intra,

IF/ICC, IP

CD282/TLR2 IMG-526 Purifi ed N/A Rabbit H, M WB

CD282/TLR2 IMG-545 Purifi ed N/A Rabbit Multi-species WB

IMG-545E Azide-Free N/A Rabbit Multi-species WB

antibodies

Cell surface fl ow cytometric analysis of CD14 in 10^6 human monocytes using 10 ul of IMG-6350H. Green represents PerCP-Cy5.5 conjugated isotype control (IMGENEX, 20109H); red represents anti-CD14 antibody. IMGENEX’s cell surface fl ow kit (10084K) was used for this test.

CD14(IMG-6350H)




CD Markers antibodies

CD282/TLR2 IMG-5651 Purifi ed N/A Rabbit H, M IHC-P, WB

CD282/TLR2 IMG-6320A Purifi ed T2.5 Mouse H, M FA-Neut, Flow-CS, IHC-Fr, IP

CD282/TLR2 IMG-662 Purifi ed N/A Rabbit M WB

CD283/TLR3 IMG-315A Purifi ed 40C1285.6 Mouse Dog, H Flow-CS+Intra, IF/ICC, IHC-P,

IP, WB

IMG-315AF488 Alexa Fluor® 488 40C1285.6 Mouse Dog, H, M Flow-CS+Intra, IF/ICC

IMG-315AF647 Alexa Fluor® 647 40C1285.6 Mouse Dog, H, M Flow-CS+Intra, IF/ICC

IMG-315B Biotin 40C1285.6 Mouse Dog, H ELISA

IMG-315C FITC 40C1285.6 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC

IMG-315D PE 40C1285.6 Mouse Dog, H Flow-CS, Flow-Intra, IF/ICC

CD283/TLR3 IMG-516 Purifi ed N/A Rabbit M Flow-CS+Intra, IHC-Fr, IHC-P, WB

IMG-516C FITC N/A Rabbit M Flow-CS, Flow-Intra

CD283/TLR3 (CT) IMG-5631 Purifi ed N/A Rabbit H, M WB

CD283/TLR3 (Tyr759) IMG-5348A PAP N/A Rabbit H IF/ICC, IHC-Fr, WB

CD284/TLR4 IMG-417A Purifi ed HTA125 Mouse Dog, H CM, FA-Neut, Flow-CS+Intra,

IF/ICC, IP

IMG-417AF488 Alexa Fluor® 488 HTA125 Mouse Dog, H CM, Flow-CS, Flow-Intra

IMG-417AF647 Alexa Fluor® 647 HTA125 Mouse Dog, H CM, Flow-CS, Flow-Intra

IMG-417B Biotin HTA125 Mouse Dog, H CM, ELISA, FA-Neut,

Flow-CS+Intra, IF/ICC, IP

IMG-417C FITC HTA125 Mouse Dog, H Flow-CS, Flow-Intra

IMG-417D PE HTA125 Mouse Dog, H CM, Flow-CS, Flow-Intra

IMG-417E Azide-Free HTA125 Mouse Dog, H CM, FA-Neut, Flow-CS+Intra,

IF/ICC, IP

CD284/TLR4 IMG-428A Purifi ed MTS510 Rat M Flow-CS, IP

IMG-428AF647 Alexa Fluor® 647 MTS510 Rat M Flow-CS

IMG-428C FITC MTS510 Rat M Flow-CS

IMG-428D PE MTS510 Rat M Flow-CS

IMG-428E Azide-Free MTS510 Rat M FA-Neut, Flow-CS, IP

CD284/TLR4 IMG-5031A Purifi ed 76B357.1 Mouse H, M, R Flow-CS+Intra, IHC-P, WB

IMG-5031AF488 Alexa Fluor® 488 76B357.1 Mouse H, M, R Flow-CS+Intra

IMG-5031C FITC 76B357.1 Mouse H, M, R Flow-CS+Intra

IMG-5031D PE 76B357.1 Mouse H, M, R Flow-CS+Intra

CD284/TLR4 IMG-577 Purifi ed N/A Rabbit H, M IP, WB

CD284/TLR4 IMG-578A Purifi ed N/A Rabbit H, M WB

CD284/TLR4 IMG-579A Purifi ed Rabbit H, M Flow-CS, IHC-Fr, IHC-P, WB

CD284/TLR4 (Tyr674) IMG-6276A PAP Rabbit H, M, R WB

CD286/TLR6 IMG-304A Purifi ed 86B1153.2 Mouse H Flow-CS, IHC-P

IMG-304AF488 Alexa Fluor® 488 86B1153.2 Mouse H Flow-CS+Intra

IMG-304AF647 Alexa Fluor® 647 86B1153.2 Mouse H Flow-CS+Intra

IMG-304B Biotin 86B1153.2 Mouse H ELISA

IMG-304C FITC 86B1153.2 Mouse H Flow-CS+Intra

IMG-304D PE 86B1153.2 Mouse H Flow-CS+Intra

CD286/TLR6 IMG-5239 Sera Rabbit H IHC-P

CD286/TLR6 IMG-527 Purifi ed N/A Rabbit H, M Flow-Intra, WB

CD286/TLR6 IMX-5260 Sera NA Rabbit H ELISA

CD288/TLR8 IMG-321A Purifi ed 44C143 Mouse H, M Flow-CS+Intra, IHC-P, WB

IMG-321AF488 Alexa Fluor® 488 44C143 Mouse H, M Flow-CS+Intra

IMG-321AF647 Alexa Fluor® 647 44C143 Mouse H, M Flow-CS+Intra IHC analysis of Kidney using IMG-71274 at 25 ug/ ml.

CD344/Frizzled-4(IMG-71274)




CD Markers

CD288/TLR8 IMG-321B Biotin 44C143 Mouse H, M ELISA

IMG-321C FITC 44C143 Mouse H, M Flow-CS+Intra

IMG-321D PE 44C143 Mouse H, M Flow-CS+Intra

CD288/TLR8 IMG-5653 Purifi ed N/A Rabbit H, M IF/ICC, WB

CD289/TLR9 IMG-305A Purifi ed 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IF/ICC,

IHC-Fr, IHC-P, WB

IMG-305AF488 Alexa Fluor® 488 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IF/ICC

IMG-305AF647 Alexa Fluor® 647 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IF/ICC

IMG-305B Biotin 26C593.2 Mouse Multi-species ELISA

IMG-305C FITC 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IF/ICC,

IHC-Fr, IHC-P, WB

CD289/TLR9 IMG-305D PE 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IHC-Fr,

IHC-P

IMG-305E Azide-Free 26C593.2 Mouse Multi-species CM, Flow-CS+Intra, IF/ICC,

IHC-Fr, IHC-P, WB

CD289/TLR9 IMG-431 Purifi ed Rabbit H, M Flow-CS+Intra, IF/ICC, IHC-Fr,

IHC-P, WB

CD290/TLR10 IMG-386A Purifi ed 158C1114 Mouse H Flow-CS+Intra, WB

IMG-386C FITC 158C1114 Mouse H Flow-CS+Intra

IMG-386D PE 158C1114 Mouse H Flow-CS+Intra

CD290/TLR10 IMG-5255A Purifi ed N/A Rabbit M, R ELISA, WB

CD290/TLR10 IMX-5093 Purifi ed N/A Rabbit H ELISA

CD290/TLR10 (IN) IMG-5630 Purifi ed N/A Rabbit H WB

E-Cadherin IMG-80129 Purifi ed 67A4 Mouse H FA-Neut, Flow-CS, IHC-Fr, WB

Leukocyte CD97 IMG-71178 PAP N/A Rabbit H IF/ICC, IHC-P

Leukocyte CD97 IMG-71179 PAP N/A Rabbit H IHC-P

Leukocyte CD97 IMG-71180 PAP N/A Rabbit H IF/ICC, IHC-P

antibodies


IL-10 IMG-222B Biotin JES5-16E3 Rat M ELISA, Flow-Intra, WB

IL-10 IMG-223A Purifi ed JES5-2A5 Rat M ELISA, ELISPOT, WB

IMG-223B Biotin JES5-2A5 Rat M ELISA, ELISPOT, WB

IL-10 IMG-317A Purifi ed JES3-9D7 Rat H ELISA, FA-Neut, Flow-Intra, WB

IMG-317E Azide Free JES3-9D7 Rat H ELISA, FA-Neut, Flow-Intra, WB

IL-10 IMG-318A Purifi ed JES3-12G8 Rat H ELISA, IHC-Fr

TIM-3 IMG-6543A Purifi ed F38-2E2 Mouse H Flow-CS

IMG-6543D PE F38-2E2 Mouse H Flow-CS

IMG-6543E Azide Free F38-2E2 Mouse H Flow-CS

T Regulatory Cellsantibodies


Dendritic Cell antibodiesInfl am

mation antibodies


Inflammation

NALP1 IMX-6337 Sera N/A Rabbit Chimp, H ELISA

NALP1 IMX-6338 Sera N/A Rabbit Multi-species ELISA

NALP1 IMG-5734 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NALP1 IMG-5735 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NALP2/PAN1 IMG-6326A PAP N/A Rabbit H IHC-P, WB

NALP2/PAN1 IMG-6370A Purifi ed N/A Rabbit H IHC-P

NALP2/PAN1 IMG-5741 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NALP2/PAN1 IMG-5742 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NALP2/PAN1 IMG-5832 Purifi ed N/A Rabbit H, M, R WB


NALP4/PAN2 IMX-6407 Sera N/A Rabbit Multi-species ELISA

NALP4/PAN2 IMG-5743 Sera N/A Rabbit H, M IHC-Fr, IHC-P, IP, WB

NALP5 IMX-6336 Sera N/A Rabbit H ELISA


NALP7 IMG-6357A PAP N/A Rabbit Multi-species WB

NALP7 IMX-6342 Sera N/A Multi-species ELISA



NALP9 IMG-6375A Purifi ed N/A Rabbit H WB


NALP10 IMG-6366A Purifi ed N/A Rabbit H WB


NALP11 IMG-6443A Purifi ed N/A Rabbit H IHC-P



NALP12/PYPAF7 IMG-5744 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NALP12/PYPAF7 IMX-6434 Sera N/A Rabbit H ELISA

OPG IMG-103A Purifi ed 98A1071 Mouse H IHC-P, WB

Spotlight on NALP1 (IMG-5735)

Formalin-fi xed, paraffi n-embedded normal human tissue sections stained for NALP1 (CARD7/NAC) using IMG-5735 at 1:2000. A, epidermis. B, esophagus. C, uterine cervix. D, thymus. Hematoxylin-eosin counterstain. In A-C, NALP1 expression is associated with differentiation in stratifi ed epithelial of the skin, esophagus, and cervix.

A B C D

antibodies




antibodies NF-B

A20 IMG-161A Purifi ed 59A426 Mouse H IP, WB

ABIN-2 IMG-434 Purifi ed N/A Rabbit H, M, R WB

ACT1 IMG-284 Purifi ed N/A Rabbit H WB

AKT1 IMG-487 Purifi ed N/A Rabbit H WB

AKT1 IMG-5411 PAP N/A Rabbit H, M, R IHC-P, WB

AKT1 (Ser473) IMG-187A Purifi ed 104A282 Mouse H, M IP, WB

AKT2 IMG-351 Ascites 95C657.1.2 Mouse H WB

AKT2/3 IMG-421 Purifi ed N/A Rabbit H WB

AKT3 IMG-326 Purifi ed 66C1247.1 Mouse H, M, R WB

AKT3 IMG-3389 PAP N/A Goat H WB

AKT3 IMG-355 Purifi ed N/A Rabbit H, M, R WB

ARAP1 IMX-6347 Sera N/A Rabbit Multi-species ELISA

ATM (Ser1981) IMG-90221-1 PAP N/A Rabbit H, M IHC-P

Bcl-3 IMG-497 Purifi ed N/A Rabbit H WB

BLyS/BAFF IMG-247 Purifi ed N/A Rabbit H, M WB

BLyS/BAFF IMG-5610 Purifi ed N/A Rabbit H, M, R WB

BMI1 IMX-6345 Sera N/A Rabbit Multi-species ELISA

CARMA1/ CARD11 IMX-5160 Sera N/A Rabbit H ELISA

CARMA1/ CARD11 IMX-5161 Sera N/A Rabbit H ELISA

Caspase-1 IMG-5025 Purifi ed N/A Rabbit H IHC-P, WB

Caspase-1 IMG-5028 Purifi ed 14F468 Mouse H, M IHC-P, WB

CIKS/Act1 IMG-563 Purifi ed N/A Rabbit H, M, R WB

CIKS/Act1 (NT) IMG-5447 PAP N/A Rabbit H WB

CUEDC2 IMG-6279A Purifi ed N/A Rabbit Multi-species WB

CUEDC2 IMG-6299A Purifi ed N/A Rabbit H IHC-P

ECSIT (CT) IMG-5618 Purifi ed N/A Rabbit H, M WB

eIF2a (Ab-51) IMG-90565-2 PAP N/A Rabbit H, M, R IHC-P, WB

eIF2a (Ser51) IMG-90331-1 PAP N/A Rabbit H, M, R IHC-P, WB

GSK3 (Ser21) IMG-90124-1 PAP N/A Rabbit H, M, R IHC-P, WB

GSK3 (Ab-216) IMG-90581-2 PAP N/A Rabbit H, M, R WB

GSK3 (Tyr216) IMG-90343-1 PAP N/A Rabbit H, M, R IHC-P, WB

HDAC2 (Ab-394) IMG-90468-2 PAP N/A Rabbit H, M, R IHC-P, WB

HDAC-4 (Ser632) IMG-90275-1 PAP N/A Rabbit H, M, R WB

HDAC5 (Ab-498) IMG-90470-2 PAP N/A Rabbit H, M, R IHC-P, WB

HDAC5 (Ser498) IMG-90276-1 PAP N/A Rabbit H, M, R IHC-P, WB

HDAC8 (Ab-39) IMG-90471-2 PAP N/A Rabbit H IHC-P, WB

HDAC8 (Ser39) IMG-90227-1 PAP N/A Rabbit H, M, R IHC-P

HNLF IMG-5112A Purifi ed N/A Rabbit M, H, R WB

IKAP IMG-4047 Purifi ed N/A Rabbit H WB

IB IMG-127A Purifi ed 6A920 Mouse H, M Flow-Intra, IHC-P, IP, WB

IB IMG-5016 Purifi ed N/A Rabbit H WB

IB IMG-5270A Purifi ed N/A Rabbit H WB

IB IMX-5142 Sera N/A Rabbit H ELISA

IB IMG-127B Biotin 6A920 Mouse H, M ELISA

IB (Ser32/Ser36) IMG-156A Purifi ed 39A1413 Mouse Multi-species ELISA, IP, WB

IMG-156B Biotin 39A1413 Mouse Multi-species ELISA

IB (Tyr305) IMG-5783 PAP N/A Rabbit H, M, R WB

IB (Tyr42) IMG-5782 PAP N/A Rabbit H, M, R IP, WB

Formalin-fi xed, paraffi n-embed-ded section of human reactive lymph node stained for Cas-pase-1 expression (IMG-5025) using a DAB chromogen and Hematoxylin counterstain.

Caspase-1(IMG-5025)




NF-B antibodies

IB (Tyr42) IMX-5097 Purifi ed N/A Rabbit Multi-species ELISA

IB (cleavage specifi c) IMG-394 Purifi ed 5D1623 Mouse H WB

IB (Ser22) IMG-90284-1 PAP N/A Rabbit H, M IHC-P, WB

IB (zeta) IMG-5098A Purifi ed N/A Rabbit M WB

IKK (Ab-23) IMG-90454-2 PAP N/A Rabbit H, M, R IHC-P, WB

IKK (Thr23) IMG-90228-1 PAP N/A Rabbit H, M, R IHC-P, WB

IKK/IKK1 IMG-136A Purifi ed 14A231 Mouse Multi-species Flow-Intra, IHC-P, IP, WB

IKK/IKK1 IMG-136AF488 Alexa Fluor® 488 14A231 Mouse Multi-species Flow-Intra

IKK/IKK1 (C1) IMG-5477 PAP N/A Rabbit H WB

IKK/IKK1 (C2) IMG-5478 PAP N/A Rabbit H WB

IKK/IKK2 IMG-129A Purifi ed 10AG2 Mouse H, M Flow-Intra, IP, WB

IKK/IKK2 IMG-129AF488 Alexa Fluor® 488 10AG2 Mouse H, M Flow-Intra

IKK/IKK2 IMG-159A Purifi ed 10A9B6 Mouse H, M IP, Flow-Intra , WB

IKK/IKK2 IMG-159AF488 Alexa Fluor® 488 10A9B6 Mouse H, M Flow-Intra

IKK/IKK2 (C3) IMG-5570 PAP N/A Rabbit H IF/ICC, WB

IKK/IKKi IMG-309 Purifi ed N/A Rabbit H WB

IKK/IKKi/TBK1 IMG-270A Purifi ed 72B587 Mouse H, M, R Flow-Intra, WB

IKK/IKK3/NEMO IMG-287A Purifi ed 46B844 Mouse H Flow-Intra, WB

IKK/IKK3/NEMO IMG-324A Purifi ed 72C627 Mouse H, M WB

IMG-324B Biotin 72C627 Mouse H, M ELISA

IKK/IKK3/NEMO IMG-4044 Purifi ed N/A Rabbit H WB

IKK/IKK3/NEMO IMG-4045 Purifi ed N/A Rabbit H WB

IKK/IKK3/NEMO IMG-5480 PAP N/A Rabbit H, M, R WB

IRAK IMG-179 Purifi ed N/A Rabbit H, M IP, WB

IRAK-2 IMG-574 Purifi ed N/A Rabbit H, M WB

IRAK-2 (C2) IMG-5495 PAP N/A Rabbit M WB

IRAK-4 IMG-441 Purifi ed N/A Rabbit H, M IP, WB

IRAK-4 IMG-5488 PAP N/A Rabbit H WB

IRS-1 (Ser639) IMG-90300-1 PAP N/A Rabbit H, M, R IHC-P

JNK2 IMG-586 Purifi ed N/A Rabbit H WB

B-Ras1 IMG-390 Purifi ed 184C278 Mouse H WB

Lck (Tyr393) IMG-90240-1 PAP N/A Rabbit H, M, R WB

LST1 IMX-6348 Sera N/A Rabbit H ELISA

MALT1 IMG-5110A Purifi ed N/A Rabbit H WB

MALT1 IMG-5745 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

MSK1 (Ab-376) IMG-90510-2 PAP N/A Rabbit H, M, R WB

MyD88 IMG-178 Purifi ed N/A Rabbit H, M, R WB

MyD88 IMG-3796 PAP N/A Goat H ELISA, IHC-P, WB

MyD88 (CT) IMG-5499 PAP N/A Rabbit H, M, R WB

MyD88 (IN) IMG-5579 PAP N/A Rabbit H IHC-P, WB


NALP7 IMG-6357A PAP N/A Rabbit Multi-species WB




NFATc1 IMG-4073 Purifi ed N/A Rabbit H WB


NFATc1 IMG-5101A Purifi ed N/A Rabbit H WB

Immunohistochemical analysis of NALP11 in formalin-fi xed, paraffi n-embedded human liver tissue using an isotype control (top) and IMG-6443A (bottom) at 5 ug/ml.

NALP11(IMG-6443A)





NF-B (p50) IMG-5845A Purifi ed N/A Rabbit Multi-species WB

NF-B (p50) IMG-5812A Purifi ed 2J10D7 Mouse H IHC-P, WB

NF-B (p65) IMG-150A Purifi ed 112A1021 Mouse H, M, R Flow-Intra, IHC-P, WB

IMG-150B Biotin 112A1021 Mouse H, M, R ELISA

NF-B (p65) IMG-512 Purifi ed N/A Rabbit H, M, R WB

NF-B (p65) IMG-5915A Purifi ed N/A Rabbit Multi-species WB

NF-B (p65) IMG-80460 Purifi ed N/A Rabbit Multi-species IHC-P

NF-B p65-NLS IMG-6066A PAP N/A Rabbit Multi-species IF/ICC, WB

NIBP IMX-5896 Sera N/A Rabbit Multi-species ELISA

NIK IMG-443 Purifi ed N/A Rabbit H WB

NOD1/CARD4 IMG-5224A Sera N/A Rabbit H WB

NOD1/CARD4 IMG-5739 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NOD2/CARD15 IMG-30161 PAP N/A Goat H WB

NOD2/CARD15 IMG-5738 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

NOD2/CARD15 IMX-5053 Sera N/A Rabbit Multi-species ELISA

OPG IMG-103A Purifi ed 98A1071 Mouse H IHC-P, WB

P38 MAPK (Thr180) IMG-90315-1 PAP N/A Rabbit H, M, R IHC-P

P38 MAPK (Tyr182) IMG-90316-1 PAP N/A Rabbit H, M, R IHC-P, WB

p50 NLS IMX-6200 Sera N/A Rabbit Multi-species ELISA

p62dok IMG-177 Purifi ed N/A Rabbit H, M WB

p65 (Ser276/281) IMX-5238 Sera N/A Rabbit H ELISA

p65-cleaved IMX-5098 Purifi ed N/A Rabbit H, M, R ELISA

p90RSK (Ab-348) IMG-90464-2 PAP N/A Rabbit H, M, R WB

p90RSK (Ab-352) IMG-90465-2 PAP N/A Rabbit H, M, R WB

p90RSK (Thr348) IMG-90208-1 PAP N/A Rabbit H, M, R IHC-P, WB

PKCb (Thr642) IMG-90260-1 PAP N/A Rabbit H, M, R IHC-P, WB

PKC (Ab-1427) IMG-90589-2 PAP N/A Rabbit H, M, R IHC-P

PKC (Thr410) IMG-90349-1 PAP N/A Rabbit H, M, R IHC-P

PKR (Ab-446) IMG-90566-2 PAP N/A Rabbit H, M IHC-P, WB

PKR (Ab-451) IMG-90571-2 PAP N/A Rabbit H, M IHC-P

PKR (Thr446) IMG-90332-1 PAP N/A Rabbit H, M IHC-P, WB

PKR (Thr451) IMG-90337-1 PAP N/A Rabbit H, M IHC-P

PLC2 (Ab-753) IMG-90500-2 PAP N/A Rabbit H, M, R WB

PLC2 (Tyr753) IMG-90263-1 PAP N/A Rabbit H, M, R WB

PRKR IMG-5120A Purifi ed N/A Rabbit H WB

PRKR IMX-5200 Sera N/A Rabbit M ELISA



RANK/TNF11aR IMG-128A Purifi ed 9A725 Mouse H, M Flow-CS, IHC-P, WB

IMG-128D PE 9A725 Mouse H, M Flow-CS+Intra

RANK/TNF11aR IMG-325A Purifi ed 64C1385.1 Mouse H, M IP, WB

RANK/TNF11aR IMG-5116A Purifi ed N/A Rabbit M, H WB

Relaxin Receptor 1 IMG-6184A Sera N/A Rabbit H IHC-P

Relaxin Receptor 1 IMX-6184 Sera N/A Rabbit H ELISA

RelB (Ab-552) IMG-90546-2 PAP N/A Rabbit H, M, R WB

NF-B

Formalin-fi xed, paraffi n- embedded tissue sections stained for TRAF6 using IMG-5766 at 1:2000. Hema-toxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow.

TRAF6(IMG-5766)

antibodies




RelB (Ser552) IMG-90318-1 PAP N/A Rabbit H, M, R IHC-P, WB

RIP IMG-5747 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

SINTBAD/TBKBP1 IMG-6172A PAP N/A Rabbit H, M, R IHC-P, WB

SINTBAD/TBKBP1 IMX-6119 Sera N/A Rabbit Multi-species ELISA

TAK1 binding protein 3 IMG-5900A Purifi ed N/A Rabbit Dog, H, M WB

TAK-1/MAP3K7 IMG-424 Purifi ed N/A Rabbit H, M WB

TAK-1/MAP3K7 (NT) IMG-5528 PAP N/A Rabbit H, M, R WB

TBK1 (Ser172) IMX-5194 Sera N/A Rabbit H ELISA

TBK1/NAK IMG-139A Purifi ed 108A429 Mouse Multi-species WB

TBK1/NAK (CT) IMG-5501 PAP N/A Rabbit H, M WB

TRADD IMG-5756 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

TRAF1 IMG-5757 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB

TRAF1 IMG-5758 Sera N/A Rabbit M IHC-Fr, IHC-P, IP, WB

TRAF2 IMG-162 Purifi ed 33A1293 Mouse H WB

TRAF2 IMG-5759 Sera N/A Rabbit H, M, R IHC-Fr, IHC-P, IP, WB


TRAF2 IMG-5761 Sera N/A Rabbit M IHC-Fr, IHC-P, IP, WB

TRAF2 and TNIK IMG-71937 PAP N/A Rabbit H IF/ICC, IHC-P

TRAF2 and TNIK IMG-71938 PAP N/A Rabbit H IHC-P

TRAF3 IMG-535 Purifi ed N/A Rabbit H, M WB


TRAF3 IMG-5763 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB


TRAF5 IMG-163A Purifi ed 55A219 Mouse H WB


TRAF6 IMG-536 PAP N/A Rabbit H, M WB


TRAF6 (CT) IMG-5654 Purifi ed N/A Rabbit H, M, R IF/ICC, WB

TRAF6BP/TAX1BP IMG-5035 Purifi ed N/A Rabbit H WB

TRAM IMG-5272A Purifi ed N/A Rabbit Chimp, H, R. Mk IHC-P, WB

Trance/RANKL IMG-133A Purifi ed 12A668 Mouse H, M IHC-P, WB

IMG-133B Biotin 12A668 Mouse H, M ELISA

IMG-133E Azide-Free 12A668 Mouse H, M IHC-P, WB

Trance/RANKL IMG-185A Purifi ed 12A380 Mouse H, M Flow-CS, IHC-Fr, WB

IMG-185D PE 12A380 Mouse H, M Flow-CS, IHC-Fr, WB

Trance/RANKL IMG-5090A Purifi ed N/A Rabbit M WB

Trance/RANKL IMG-5108A Purifi ed N/A Rabbit M WB

TRIB3/SKIP3/SINK IMG-5118A Purifi ed N/A Rabbit H WB

Ubiquitin IMG-5021 Ascites Ubi-1 Mouse Multi-species IF/ICC, IHC-P, WB

Ubiquitin IMG-80150 T.C. Sup. FPM1 Mouse H IHC-P

Ubiquitin IMG-80385 Purifi ed N/A Rabbit Multi-species IHC-P

Ubiquitin IMG-5020 Sera N/A Rabbit Multi-species IF/ICC, WB

Zap-70 (Ab-319) IMG-90491-2 PAP N/A Rabbit H, M, R IHC-P, WB

Zap-70 (Ab-493) IMG-90492-2 PAP N/A Rabbit H, M, R IHC-P

Zap-70 (Tyr319) IMG-90252-1 PAP N/A Rabbit H, M, R WB

Zap-70 (Tyr493) IMG-90253-1 PAP N/A Rabbit H, M, R IHC-P

NF-B antibodies

Immunohistochemical staining of Trance/RANKL/OPGL/ODF in formalin-fi xed, paraffi n-embedded human lymph node using IMG-133A at 5 ug/ml. Hematoxylin-eosin counterstain.

Trance/RANKL(IMG-133A)


antibodies Dendritic CellCytokines & Chemokinesantibodies


Asb-2 IMG-502 Sera N/A Rabbit H WB

Asb-11 IMG-504 Purifi ed N/A Rabbit H WB

BAFF-R (CT) IMG-5553 PAP N/A Rabbit H, M, R IHC-P, WB

Bonzo (CT) IMG-5591 Purifi ed N/A Rabbit H WB

Bonzo (NT2) IMG-5430 PAP N/A Rabbit H WB

CAMK 1D- IMG-5008 Purifi ed N/A Rabbit H WB

CCR5 IMG-333 Purifi ed N/A Rabbit H WB

CCR10 IMG-286 Purifi ed N/A Rabbit H Flow-Intra, IHC-Fr, WB

CTRP2 IMG-5454 PAP N/A Rabbit H, M, R WB

CTRP7 IMG-5463 PAP N/A Rabbit H, M, R WB

CTRP7 (CT) IMG-5461 PAP N/A Rabbit H, M, R WB

CX3CR1 IMG-183 Purifi ed N/A Rabbit H, M, R IHC-P, WB

CX3CR1 (EL) IMG-5464 PAP N/A Rabbit H WB

CXCR1/CD191 IMG-329 Purifi ed N/A Rabbit H, M Flow-CS, WB

CXCR1/IL-8R A IMG-334 Purifi ed N/A Rabbit H WB

CXCR3 IMX-6202 Sera N/A Rabbit H Peptide ELISA*

CXCR3 IMG-331A Purifi ed N/A Rabbit H, M, R WB

IMG-331E Purifi ed N/A Rabbit H, M, R WB

CXCR3 (CCR3) IMG-72169 PAP N/A Rabbit H IHC-P

CXCR4/CD194 IMG-332 Purifi ed N/A Rabbit H, M, R WB

CXCR4 IMG-126 Purifi ed N/A Rabbit H, M WB

CXCR4 IMG-537 Purifi ed N/A Rabbit H, M, R IHC-P, WB

CXCR4 (NT) IMG-125 Purifi ed N/A Rabbit H, M IF/ICC, WB

CXCR5 IMG-327 Purifi ed N/A Rabbit H WB

EBI3 IMG-6100A Purifi ed N/A Rabbit Chimp, H WB

Eotaxin (CT) IMG-5594 Purifi ed N/A Rabbit H WB

FAM3C IMG-5989A PAP N/A Rabbit Multi-species IHC-P, WB

GPR81/FKSG80 IMG-71232 PAP N/A Rabbit H, R IHC-P

GPR81/FKSG80 IMG-72204 PAP N/A Rabbit H IHC-P

GPR81/FKSG80 IMG-72205 PAP N/A Rabbit H IHC-P

GITRL IMG-5474 PAP N/A Rabbit H, M WB

GM-CSF IMG-226A Purifi ed MP1-22E9 Rat M ELISPOT, FA-Neut, Flow-Intra,

IHC (cryosections), WB

IMG-226E Azide-Free MP1-22E9 Rat M ELISPOT, FA-Neut, Flow-Intra,

IHC (cryosections), WB

GM-CSF IMG-227A Purifi ed MP1-31G6 Rat M ELISPOT, IHC (cryosections),

IP, WB

IFN- IMG-209A Purifi ed 144BS Mouse H ELISA, Flow-CS, IP

IFN- IMG-210A Purifi ed 43-11 Mouse H CITA, CLIA, FA-Neut

IMG-210E Azide-Free 43-11 Mouse H CITA, CLIA, FA-Neut

IL-1 Ra IMG-203A Purifi ed 1.384 Mouse H CLIA, FA-Neut, IHC-Fr

IL-1b IMG-212A Purifi ed 1400.24.17 Rat M FA-Neut

IMG-212E Azide-Free 1400.24.17 Rat M FA-NeutIHC analysis of Pituitary, Anterior using IMG-71232 at 3 ug/ ml.

GPR81/FKSG80(IMG-71232)

NEW


Dendritic Cell antibodiesCytokines & Chem

okines antibodies


Cell-surface fl ow cytometry analysis of IL-24 in Jurkat cells using IMG-5223A at 5 ug/10^6 cells. The shaded histogram represents cells only, green represents isotype control, and purple represents anti-IL-24 antibody.

IL-24/MDA-7(IMG-5223A)

IL-1RAcP (C1) IMG-5481 PAP N/A Rabbit H WB

IL-1RAcP (C2) IMG-5482 PAP N/A Rabbit H WB

IL-2 IMG-204A Purifi ed N7.48A Mouse H Flow-Intra, IF/ICC,

IHC (cryosections)

IMG-204C FITC N7.48A Mouse H Flow-Intra, IF/ICC,

IHC (cryosections)

IL-4 IMG-206A Purifi ed 4D9 Mouse H FA-Neut, IF/ICC, WB

IMG-206E Azide-Free 4D9 Mouse H FA-Neut, IF/ICC, WB

IL-4 IMG-207A Purifi ed 8F12 Mouse H Flow-Intra, IF/ICC, WB

IMG-207E Azide-Free 8F12 Mouse H FA-Neut, Flow-Intra, IF/ICC, WB

IL-6 IMG-220A Purifi ed MP5-20F3 Rat M ELISPOT, IF/ICC, IP

IMG-220D PE MP5-20F3 Rat M ELISPOT, Flow-Intra, IF/ICC, IP

IMG-220E Azide-Free MP5-20F3 Rat M ELISPOT, IF/ICC, IP

IL-6 IMG-221A Purifi ed MP5-32C11 Rat M ELISPOT, IF/ICC, IP

IMG-221B Biotin MP5-32C11 Rat M ELISPOT, IF/ICC, IP

IL-10 IMG-222B Biotin JES5-16E3 Rat M Flow-Intra, WB

IL-10 IMG-223A Purifi ed JES5-2A5 Rat M ELISPOT, WB

IL-10 IMG-317A Purifi ed JES3-9D7 Rat H FA-Neut, Flow-Intra, WB

IMG-317E Azide-Free JES3-9D7 Rat H FA-Neut, Flow-Intra, WB

IL-10 IMG-318A Purifi ed JES3-12G8 Rat H IHC-Fr

IL-17A IMG-6545A Purifi ed 4K5F6 Mouse H Flow-Intra, WB

IMG-6545A647 Alexa Fluor® 647 4K5F6 Mouse H Flow-Intra

IMG-6545B Biotin 4K5F6 Mouse H Flow-Intra, WB, ELISA

IMG-6545D PE 4K5F6 Mouse H Flow-Intra

IL-17B/E R IMG-345A Purifi ed 97C691 Mouse H WB

IL-17B/E R IMG-470A Purifi ed N/A Rabbit H WB

IL-17E IMG-323A Purifi ed 68C1039.2 Mouse H, M WB

IL-18RA/AcPL IMG-5353A Purifi ed NA Rabbit H, M WB

IL-19 IMX-5182 Sera N/A Rabbit H Peptide ELISA*

IL-19 IMX-5198 Sera N/A Rabbit H Peptide ELISA*

IL-20RA IMG-5226A Purifi ed N/A Rabbit H, M WB

IL-20RA IMX-5179 Sera N/A Rabbit H Peptide ELISA*


IL-21 (IN) IMG-5572 PAP N/A Rabbit H IF/ICC, WB

IL-21R IMG-547 Purifi ed N/A Rabbit H WB

IL-21R (NT) IMG-5484 PAP N/A Rabbit H, M, R WB




IL-22R (NT) IMG-5573 PAP N/A Rabbit H, R IF/ICC, WB

IL-23R IMG-5092A Purifi ed N/A Rabbit H, M IHC-P, WB

IL-23R IMX-5938 Sera N/A Rabbit Chimp, H Peptide ELISA*

NEW

NEW

NEW

NEW


antibodies Dendritic CellScreening Services for Inflammation & Immune Signaling Pathways

IMGENEX is a recognized leader in the provision of tools for studying cellular responses. We have experi-ence in the manufacturing of reagents and development

of applications and assay systems for studying pathways of cellular response, particularly pathways related to Im-mune Receptors with a focus on Toll-like Receptors. Over the past several years IMGENEX has developed a broad product portfolio in the area of Toll-like Receptors (TLRs), and related areas of Infl ammation and Immunology pro-viding advanced research tools to accelerate the increas-ing volume of applied research in these areas of biology.

In parallel with their critical role in shaping and regu-lating cellular and immune responses, is the recogni-tion of Toll-like Receptors as tunable targets to control these responses. Identifying new, more specifi c and more potent TLR agonists or antagonists is an area of

focus for companies with intent for commercial develop-ment of diagnostics for monitoring response, or inter-ested in therapeutics as prophylactic or curative drugs.

To help meet these objectives we now offer our new IM-GENEX Screening Services as a partner with expertise in cell signaling areas, as evidenced by the portfolio of prod-ucts already commercialized. We are recognized as au-thorities in cell signaling pathways especially in the area of NF-B related signaling pathways which are directly related to Infl ammation and Immune Signaling Pathways.

Through IMGENEX Screening Services our objective is to become your partner in accelerating your research and product development efforts and meeting your spe-cifi c objectives effi ciently and economically in accord with the scope, resources, and timetable structure desired.

TLR & Infl ammatory Ligand Discovery Screen • Identifi cation of TLR ligands in cell culture • Identifi cation of TLR ligands in tissue fl uids • Identifi cation of TLR specifi c ligands

NF- B SEAPorter™ Assay Kit (Cat. No. IMK-515)

The IMGENEX NF- B SEAPorter™ Assay Kit is designed to provide a flexible read-out system for the measurement of NF- B activation, by ELISA compatible techniques, via quantitative measurement of secreted alkaline phosphatase in cell supernatants.

The NF- B SEAPorter™ Assay Kit offers

plasmids containing SEAP cDNA, buffers, and substrate.

MyD88

TLR1orTLR6

TIRAP

MyD88 MyD

88

TIRAP

TRIF

TRAM

TRIF

MD-2

CD

14 TLR4 TLR

3

TLR2

TLR5

TLR7

TLR8

TLR9or

TLR11

IRAK1/4 TRAF6

MAPKs IKKs

AP-1I B NF- B

NF- B

TBK1 IKK

IRF-3

SEAP

NF- B Transfected HEK 293Stable Cells (Cat. No. IML-101)

NF- B inducible secreted alkaline

phosphatase gene-designed as an optimal

choice of cells for TM

Assay Kit.

TLR & Infl ammatory Ligand Activation Screen • Pathway Analysis • Activation Potential

TLR & Infl ammatory Pathway Analysis • Mediator Cascade Analysis • Cytokines • Chemokines • Prostaglandins • Interferons

TLR3TLR4

TLR7TLR8TLR9

TLR2TLR3IL-1

IL-1

TLR1TLR2TLR4TLR5TLR6IL-1

TLR7TLR8TLR9TLR10TLR11

TLR3TLR4TLR5

NOD1 NOD2 TLR2

RECE

PTO

RPA

THW

AYRE

AD-O

UT

IRF3 IRF7 ROS CRE-AP-1 NF-B

IRF3

IRF7

ROS

IL-1

MyD8

8

P13K

RIP1

NOD1

NOD2

RIP2TRAF6

TRAF2

Partnering Information & Initiation of IMGENEX Screening Services Project

1Contact IMGENEX Screening Services

Tel: 858-642-0978 x 244Toll-Free: 888-723-4363 x [email protected]

2Assurance of Confi dentiality

Exchange NDA assures confi -dentiality

3Discussion of Overall Project

Project Scope, Timetable, Objectives

4IMGENEX provides SOW- Statement of Work

Systems, Assays, Reagents Planned

Data Analysis Formats and Requirements

Timetable & MilestonesQuotation Presentation

5In Depth Discussion and Project Review

Review and revise SOW as agreed upon

6Agreement and Go Ahead

Start partnering with IMGENEX

11175 Flintkote Avenue, Suite E

San Diego, CA 92121

Toll-free (888) 723-4363

Fax (858) 642-0937

Email [email protected]

www.imgenex.com

www.imgenex.com

Documents

Dendritic Cell Research Tools, Product Flyer