DAFTAR PUSTAKAeprints.undip.ac.id/46201/9/Monica_Adyah_Permata... · 2015. 8. 28. · Expanding maternal and neonatal survival (EMAS) 2012 – 2016: Kementrian Kesehatan Republik

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LAMPIRAN

Lampiran 1. Ethical Clearance

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Lampiran 2. Izin penelitian

65

Lampiran 3. Informed Consent

66

Lampiran 3. Informed Consent (lanjutan)

67


68


Lampiran 4. Prosedur pembuatan preparat jaringan

69

Pengecatan : Hematoksilin Eosin

1. Tali pusat dipotong sepanjang 2 cm.

Potongan tali pusat diimasukkan ke dalam pot penampung

berisi buffer formalin 10% dengan perbandingan volume

jaringan: formalin adalah 1:10.

Pemberian identitas pot penampung jaringan.

Pengiriman sampel ke laboratorium sentral bagian Patologi

Anatomi.

2. Jaringan mentah dipotong menjadi 3 bagian (dipilih bagian

yang sesuai dengan kriteria penelitian).

Potongan jaringan dimasukkan dalam kaset.

Kaset jaringan kemudian direndam kembali dalam wadah

berisi formalin 10% hingga Processing jaringan siap

dilakukan.

3. Kaset jaringan dimasukkan ke dalam keranjang alat processing

jaringan yakni Microm STP 120 (1 keranjang bisa memuat hingga 80

kaset). Processing jaringan selama 17, 5 jam.

Microtom STP 120 terdiri dari beberapa tabung, yaitu :

Formalin 10% 1, lama perendaman 2 jam

Formalin 10% 2, lama perendaman 2 jam

Alkohol 70 % , lama perendaman 1 jam

Alkohol 80%, lama perendaman 1 jam

Alkohol 96%, lama perendaman 2 jam

Etanol, lama perendaman 2 jam

Xylol, lama perendaman 1 jam

Parafin 1, lama perendaman 2 jam



Tujuan processing:

Menghilangkan cairan pada jaringan (dehidrasi dg alkohol

bertingkat yakni : alkohol 70%,80%,96% dan etanol).

Xylol untuk clearing.

Parafin untuk mematangkan jaringan.

4. Pembuatan blok jaringan matang dengan alat Histocenter . Digunakan

cairan paraffin panas yang kemudian didinginkan untuk membekukan

blok jaringan.

70

5. Blok jaringan dipotong dengan mikrotom (ketebalan 2 mikron).

Hasil pemotongan dimasukkan dalam air hangat agar

mengembang, kemudian jaringan ditempelkan pada kaca objek.

Preparat diberi identitas

6. Preparat dipanaskan dalam oven 70 derajat selama 10 menit

(untuk melelehkan parafin)

Setelah selesai preparat dinginkan, kemudian ditata pada rak

jaringan untuk persiapan proses pengecatan.

7. Pengecatan dilakukan dengan alat Veristein dengan total waktu 2,5

jam. Veristein terdiri dari beberapa tabung, yaitu :

Xylol 1, lama perendaman 10 menit



Etanol , lama perendaman 3 menit

Alkohol 96% , lama perendaman 3 menit

Alkohol 80% , lama perendaman 3 menit

Alkohol 70%, lama perendaman 3 menit


Aqua

Hematoksilin , lama perendaman 15 menit

HCL, lama perendaman 1 menit

Bruing, lama perendaman 10 menit

Aqua, lama perendaman 3 menit



Eosin, lama perendaman 3 menit



Etanol, lama perendaman 3 menit




Fungsi masing-masing cairan :

Xylol berfungsi untuk melarutkan lilin

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Aqua, merupakan pelarut hematoksilin fungsi menyamakan pH

dengan hematoksilin

Hematoksilin berfungsi untuk mewarnai inti

HCL berfungsi untuk mencegah pewarnaan berlebih, agar

pewarnaan tidak terlalu biru

Bruing berfungsi untuk memperjelas warna inti

Alkohol 50% dan 70%, merupakan pelarut eosin

Eosin, berfungsi untuk mewarnai sitoplasma

Alkohol bertingkat, untuk rehidrasi jaringan

Xylol untuk proses clearing

8. Preparat yang sudah diwarnai ditetesi Ez Mount kemudian ditutup

dengan deck glass .

Ez Mount berfungsi untuk merekatkan deck glass dan mengawetkan

reparat jaringan (dengan pengawetan ini bisa disimpan hingga 10

tahun)

72

73

74

Lampiran 6. Hasil analisis statistik

Explore

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

Umur ibu 32 100.0% 0 0.0% 32 100.0%

Umur kehamilan 32 100.0% 0 0.0% 32 100.0%

Cara persalinan 32 100.0% 0 0.0% 32 100.0%

Paritas 32 100.0% 0 0.0% 32 100.0%

Tests of N ormality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

Umur ibu .135 32 .143 .941 32 .079

Umur kehamilan .254 32 .000 .852 32 .000

Cara persalinan .282 32 .000 .753 32 .000

Paritas .402 32 .000 .615 32 .000

a. Lilliefors Significance Correction

T-Test

Group Statistics

Diagnosis N Mean Std. Deviation Std. Error Mean

Umur ibu Preeklampsia berat 16 33.00 6.563 1.641

Kehamilan normotensi 16 28.31 5.582 1.396

Independent Samples Test

Levene's

Test for

Equality of

Variances

t-test for Equality of Means

F

Sig

.

t Df

Sig

. (2

-tai

led)

Mea

n

Dif

fere

nce

Std

. E

rro

r

Dif

fere

nce

95% Confidence

Interval of the

Difference

Low

er

Upper

Umur

ibu

Equal

variances

assumed .084 .774 2.176 30 .038 4.688 2.154 .289 9.086

Equal

variances

not

assumed

2.176 29.248 .038 4.688 2.154 .284 9.091

75

Lampiran 6. Hasil analisis statistik (lanjutan)

Mann-Whitney Test

Test Statisticsa

Umur kehamilan Cara persalinan Paritas

Mann-Whitney U 85.500 123.500 80.000

Wilcoxon W 221.500 259.500 216.000

Z -1.697 -.182 -2.156

Asymp. Sig. (2-tailed) .090 .855 .031

Exact Sig. [2*(1-tailed Sig.)] .110b .867b .073b

a. Grouping Variable: Diagnosis

b. Not corrected for ties.

Means

Report

Diagnosis Umur ibu Umur kehamilan

Preeklampsia berat

Mean 33.00 38.06

Std. Deviation 6.563 .854

Median 35.00 38.00

Minimum 18 37

Maximum 40 39

Kehamilan normotensi

Mean 28.31 38.88

Std. Deviation 5.582 1.258

Median 27.00 38.00

Minimum 20 38

Maximum 39 42

Total

Mean 30.66 38.47

Std. Deviation 6.449 1.135

Median 32.00 38.00

Minimum 18 37

Maximum 40 42

Explore


Cases

Valid Missing Total


Luas area tali pusat 32 100.0% 0 0.0% 32 100.0%

Luas area pembuluh darah 32 100.0% 0 0.0% 32 100.0%

Luas area Wharton's jelly 32 100.0% 0 0.0% 32 100.0%

76


Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

Luas area tali pusat .198 32 .003 .879 32 .002

Luas area pembuluh darah .202 32 .002 .845 32 .000

Luas area Wharton's jelly .180 32 .010 .875 32 .002

a. Lilliefors Significance Correction

Mann-Whitney Test

Test Statisticsa

Luas area tali pusat Luas area pembuluh

darah Luas area Wharton's jelly

Mann-Whitney U 61.000 41.000 67.000

Wilcoxon W 197.000 177.000 203.000

Z -2.525 -3.279 -2.299

Asymp. Sig. (2-tailed) .012 .001 .022

Exact Sig. [2*(1-tailed Sig.)] .011b .001b .021b

a. Grouping Variable: Diagnosis

b. Not corrected for ties.

Means


Cases

Included Excluded Total


Luas area tali pusat *

Diagnosis 32 100.00% 0 0.00% 32 100.00%

Luas area pembuluh darah *

Diagnosis 32 100.00% 0 0.00% 32 100.00%

Luas area Wharton's jelly *

Diagnosis 32 100.00% 0 0.00% 32 100.00%

77


Report

Diagnosis Luas

area tali pusat

Luas area pembuluh

darah Luas area Wharton's jelly

Preeklampsia berat

Mean 85.2857 12.6448 72.6410

Std. Deviation

38.14485 4.23282 35.68374

Median 81.9518 11.8321 70.1197

Minimum 30.81 7.19 23.62

Maximum 154.16 20.69 135.46

Kehamilan normotensi

Mean 52.0836 8.4818 43.6018

Std. Deviation

16.45145 2.27845 15.19417

Median 46.3182 7.8793 38.2380

Minimum 30.80 6.41 23.74

Maximum 89.18 15.91 79.07

Total

Mean 68.6847 10.5633 58.1214

Std. Deviation

33.45881 3.95647 30.74823

Median 53.4325 9.0636 45.7462

Minimum 30.80 6.41 23.62

Maximum 154.16 20.69 135.46

78

Lampiran 7. Dokumentasi

Lampiran 6. Dokumentasi (lanjutan)

Lampiran 6. Dokumentasi (lanjutan)

Gambar 9. Pengambilan sampel tali pusat

Gambar 10. Processing jaringan

Gambar 11. Preparat penelitian

79

Gambar 12. Pembacaan dan perhitungan preparat

80

Lampiran 8. Biodata mahasiswa

Identitas

Nama : Monica Adyah Permata

NIM : 22010111120033

Tempat/tanggal lahir : Karawang, 25 September 1992

Jenis kelamin : Wanita

Alamat : Gg. Madi 2, RT01/01, Ds.Pucung, Kec. Kotabaru,

Kab. Karawang, Jawa Barat

Nomor Hp : 083875162522

e-mail : [email protected]

Riwayat Pendidikan Formal

1. SD : SD Negeri Cikampek Utara II Lulus tahun :2004

2. SMP : SMP Negeri II Cikampek Lulus tahun :2007

3. SMA : Hopewell High School Lulus tahun :2010

SMA Negeri 1 Karawang Lulus tahun :2011

4. FK UNDIP : Masuk tahun : 2011

Keanggotaan Organisasi

1. Staf Hubungan Luar HIMA KU Tahun : 2011 s.d. 2014

2. Ketua Financial ROHIS KU Tahun : 2012 s.d. 2013

3. Paduan suara mahasiswa KU Tahun : 2011 s.d. 2014

4. Volunteer Bina Antar Budaya Tahun : 2011 s.d. sekarang

Pengalaman Penelitian

-

mailto:[email protected]

Documents

DAFTAR PUSTAKAeprints.undip.ac.id/46201/9/Monica_Adyah_Permata... · 2015. 8. 28. · Expanding maternal and neonatal survival (EMAS) 2012 – 2016: Kementrian Kesehatan Republik