Waste Map: Nelson Lab mycotoxin experiments and waste streamsCreated by Henry Wells on 4 June 2016

Page 1: Waste generated during sorting and testing activities in 153 Dimock lab:* Note: Orange boxes contain, or have come into contact with aflatoxin, and require disposal via AF waste streams* Note: Blue boxes contain materials that have not been contaminated with AF* Note: Green boxes do not refer to any physical materials – rather, they represent a process within waste generation

Page 2: Waste generated during sorting and testing activities in 303G Plant Sciences Building lab:

* Note: Orange boxes contain, or have come into contact with aflatoxin, and require disposal via AF waste streams

* Note: Blue boxes contain materials that have not been contaminated with AF

Contaminated glass test tubes and/or macro-pipette tips

Final waste destination:

Liquid waste from assays (toxin extract + reagents)

Contaminated gloves, paper towels, assay cups, falcon tubes, AF and FUM dipsticks, pipette tips, Ziploc bags used to hold excess ground maize

Excess extract solution (original and dilutions)

Testing samples for Aflatoxin and Fumonisin

AMBER LIQUID WASTE JUG (vented cap, combined with 15% v:v of 5.25% bleach solution)

Additional solvent and tubes used for dilutions

CHEMOTHERAPY SHARPS BIN (yellow, with biohazard symbol)

Additional pipette tips, towels, gloves

CHEMOTHERAPY WASTE BAG (yellow, with biohazard symbol)

Contaminated weigh boats, paper towels, pipette tips, gloves

mReader test kits: AF and/or FUM dipsticks, assay cups

Toxin extract: solvent + ethanol-maize ‘slurry’, in glass and/or plastic tubes

Weigh boats, paper towels, pipette tips, gloves used during sample prep.

Extraction of toxins for testing

65% Ethanol extract solvent

Glass test tube or polypropylene falcon tube

Ground Maize Samples

* Note: Green boxes do not refer to any physical materials – rather, they represent a process within waste generation

For disposal of contaminated

waste:

1. Chemotherapy waste bags should be sent for incineration. Until EHS can pick them up, store in red biohazardous waste bin in lab (there will be one in both labs). Arrange for pick- up at:

http://oldsite.ehs.cornell.edu/rad/MedWasteForm.cfm

Additional materials for HPLC: autosampler vials, syringes & filters

ELISA test kits: mixing wells, antibody-coated wells, reagents

Contaminated glass test tubes and/or macro-pipette tips

Final waste destination:

Liquid waste from assays (toxin extract + reagents)

Contaminated gloves, paper towels, assay cups, falcon tubes, AF and FUM dipsticks, pipette tips, Ziploc bags used to hold excess ground maize

Excess extract solution (original and dilutions)

Testing samples for Aflatoxin and Fumonisin

AMBER LIQUID WASTE JUG (vented cap, combined with 15% v:v of 5.25% bleach solution)

Additional solvent and tubes used for dilutions

CHEMOTHERAPY SHARPS BIN (yellow, with biohazard symbol)

Additional pipette tips, towels, gloves

CHEMOTHERAPY WASTE BAG (yellow, with biohazard symbol)

Contaminated weigh boats, paper towels, pipette tips, gloves

mReader test kits: AF and/or FUM dipsticks, assay cups, reagents

Toxin extract: solvent + ethanol-maize ‘slurry’, in glass and/or plastic tubes

Weigh boats, paper towels, pipette tips, gloves used during sample prep.

Extraction of toxins for testing

65% Ethanol (mReader) or 80% Methanol (ELISA, HPLC) extract solvent

Glass test tube or polypropylene falcon tube

Ground Maize Samples

2. Contents of chemotherapy sharps containers should also be sent for incineration. Arrange for pick-up via the same link in #1.

3. Contents of amber waste jugs (i.e. all liquid waste generated during experimental process – toxin extract, maize slurry, liquid waste from assay reagents) should be combined with a 5.25% bleach (sodium hypochlorite) solution at 15% bleach v : v 85% liquid waste. Arrange for pick-up of full jugs here: https://sp.ehs.cornell.edu/training/Pages/default.aspx. These should be labeled with a Cornell EHS hazardous waste form such as the one in the image on the right: