Course Overview Biology of Mammalian Cells and Tissues - Histology VIBS 443/ VIBS 602 Larry Johnson Cells Tissues Organs/systems Objectives To make a transition from molecular events to organ systems. To have classical histology merged with modern cell biology. To learn technical skills - new types of vision - using the light microscope and electron microscope. Objectives To learn the relationship between cell structure and cell function, and how organ architecture facilitates its function. To learn normal histology as a prerequisite for understanding pathology. To learn how to communicate and have fun with histology. Materials Laboratory notebook file, study guides, and items to identify Course schedule Computers to view histology images, electron micrographs, and their care PowerPoint slides to introduce each laboratory, conference, and lecture Labeled electron micrographs Atlas images diFiores Atlas of Histology w/ Functional Correlations 12 th edition Introductory to labs YouTube VIBS Histology Introductory to labs PPTs VIBS Histology Introductory to labs PPTs VIBS Histology To view the Imagescope files on students computers in computer labs 1. Login as a vet or grad student or if undergrad, use the ID of cvmlab with the password of AStud3nt. 2. Wait for the icons on the lower right corner of the task bar to appear (usually around 5 icons). 3. Click on Start, VIBS folder, Imagescope 4. Once the program loads, Click on File, Open Image 5. Change to the X:\classes drive 6. Click on "Shortcut to LJohnsonHistologySlides 7. Choose the file you want to view. Hence, you will login, load program, load files, and select file. Materials Lecture outlines, laboratory exercises, word slides, and study guides for each lecture. [Note that some of these materials are modified from copyrighted textbooks (see last page for original sources) and should not be copied for non-personal use.] Evaluations - must be returned to receive final exam. Materials Textbooks Junqueiras Basic histology Text & Atlas by Anthony L. Mescher 13e (ISBN ) Earlier versions will likely work too as the textbook materials will be used as a supplement source of information. Organization Cell Biology - Tissues - Organs - Organ Systems Lectures: (26) Begin usually at 10:20 A.M. are supplemented by five clinical correlations to relate lectures material to pathological conditions. The objective of lectures is to learn about structure - function relationships and fundamental concepts. Laboratories: To learn cell and tissue appearance and function. Subject matter of laboratories usually will be that material covered in lecture of the previous class period. Conferences: (10) To develop conceptual thinking for problem solving. Organization Preparative homework allows the student to become familiar with the subject of the lecture prior to the lecture period. Preparative homework (25 sets total) is due prior to the lecture on the day that the subject matter is covered in the lecture. Organization The homework will consist of a list of 3 to 5 structures or structural features unique or specifically characteristic of cells, tissues, organs, or organ systems covered in the lecture that day, and one sentence for each describing how these structures or structural features contribute to the unique function of that given cell, tissue, organ, or organ system. Possibly, a randomly-selected subset of homework will be graded; however, all homework submitted on time will be recorded. Parts of course will be FLIPPED for more class time discussions. You will watch short videos in preparation for class. Lab introductions to set up the observation of images as directed within the lab manual Introductions to conferences Lab introductions to set up the observation of images as directed within the lab manual Introductions to conferences FLIP = FLoating Instrument Platform Exams: Quiz to test all materials covered since last quiz or didactic test Practical exams to test concepts and identifications Essay exams on conference material Didactic exams to test everything Writing Assignments Term Paper Objectives To advance understanding of conference topics as they relate to real-world situations To improve communication skills of students in medical fields To improve awareness of issues related to common diseases Writing Assignments Term Paper Prompt Choose one of the discussion conference topics about which to compose your essay. Assume this will be published in a scientific journal and will be read by your colleagues. Use outside sources, which should be cited correctly at the end of the paper, and describe the implications of your topic to the medical field. The final product should be words (not to exceed 3,500 words), typed and double spaced, in Times New Roman, with 1 margins. Be sure to include the title of the discussion conference in the paper and a list of your sources at the end. If you use a direct quotation, you must correctly cite it in the paper. Writing Assignments Submittals The following will be due throughout the semester. Feedback will be given at each step, with a final grade given for the final draft. Outline Description First Draft Second Draft Final Draft Grading of Term Paper Submitting of each paper on time 10 points First and Second Draft: 40 points Final Draft: 100 points Total points of course: 150 points H.Grading 1.Point distribution Point Values a.Preparative homework100 b.Quiz (6) every other week200 (50 points each, drop 2) c.Term Paper150 d.Mid-term i.Practical portion100 ii.Conference essay portion 25 iii.Didactic portion150 e.Final i.Practical portion100 ii.Conference essay portion25 iii.Didactic portion150 Total A=900+; B= ; C= ; D= ; F=599-0 Organization Student participation in class and student enhancement of the objectives of the course are encouraged and may pay off in borderline cases. Scholastic dishonesty including plagiarism as defined at the TAMU Web Site (http://student- rules.tamu.edu/rules20.htm) will not be allowed. Grades may be curved at the end of the semester. Getting Started in the Laboratory Light microscope parts Kohler illumination page 28 field diaphragm 1.Focus on the tissue specimen using the course/fine focus knobs. 2. Close the field diaphragm. 3.Focus the condenser (e.g., image of the closed field diaphragm) on the specimen using the condenser height adjustment knob. 4.Center the condenser (e.g., image of the field diaphragm) with the two condenser centering knobs so that the light passing through the opening of the field diaphragm is concentric with the peripheral edges of view. 5.Open the field diaphragm so that field diaphragm is just outside of view. Kohler illumination Kohler illumination 1.Focus on the tissue. 2.Close the field diaphragm. 3.Focus the condenser. 4.Center the condenser 5. Open the field diaphragm. Focus on the tissue Close the field diaphragm Focus the condenser Center the condenser Open the field diaphragm Cartilage growth in endochondral bone formation of the Fetal finger 220 Fingertip, monkey - sweat glands and ducts among Pacinian corpuscles 105 Skin, scalp 209 Skeletal muscle of Tongue, monkey 136 Skin, melanocytes, guinea pig (DOPA reaction) 107 Birds on the Move Backgrounds Rockport Birds on the Wing