Copyright 2009 Pearson Education, Inc. Art and Photos in PowerPoint Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 10 DNA Structure and Analysis Copyright 2009 Pearson Education, Inc. 10.1The Genetic Material Must Exhibit Four Characteristics Replication Storage of information Expression of information Variation by mutation Copyright 2009 Pearson Education, Inc. Figure 10.1 Copyright 2009 Pearson Education, Inc. 10.2Until 1944, Observations Favored Protein as the Genetic Material Copyright 2009 Pearson Education, Inc. Figure 10.2 Copyright 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transformation: Early Studies Copyright 2009 Pearson Education, Inc. Figure 10.3 Copyright 2009 Pearson Education, Inc. Table 10.1 Copyright 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transformation: The Avery, MacLeod, and McCarty Experiment Copyright 2009 Pearson Education, Inc. Figure 10.4 Copyright 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages The HersheyChase Experiment Copyright 2009 Pearson Education, Inc. Figure 10.5 Copyright 2009 Pearson Education, Inc. Figure 10.6 Copyright 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transfection Experiments Copyright 2009 Pearson Education, Inc. 10.4Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes Indirect Evidence: Distribution of DNA Indirect Evidence: Mutagenesis Copyright 2009 Pearson Education, Inc. Table 10.2 Copyright 2009 Pearson Education, Inc. Figure 10.7 Copyright 2009 Pearson Education, Inc. 10.4Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes Direct Evidence: Recombinant DNA Studies Copyright 2009 Pearson Education, Inc. 10.5RNA Serves as the Genetic Material in Some Viruses Copyright 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Nucleotides: Building Blocks of Nucleic Acids Copyright 2009 Pearson Education, Inc. Figure 10.9 Copyright 2009 Pearson Education, Inc. Figure 10.9a Copyright 2009 Pearson Education, Inc. Figure 10.9b Copyright 2009 Pearson Education, Inc. Figure 10.10 Copyright 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Nucleoside Diphosphates and Triphosphates Copyright 2009 Pearson Education, Inc. Figure 10.11 Copyright 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Polynucleotides Copyright 2009 Pearson Education, Inc. Figure 10.12 Copyright 2009 Pearson Education, Inc. Figure 10.12a Copyright 2009 Pearson Education, Inc. Figure 10.12b Copyright 2009 Pearson Education, Inc. 10.7The Structure of DNA Holds the Key to Understanding Its Function Base-Composition Studies X-Ray Diffraction Analysis Copyright 2009 Pearson Education, Inc. Table 10.3 Copyright 2009 Pearson Education, Inc. Figure 10.13 Copyright 2009 Pearson Education, Inc. 10.7The Structure of DNA Holds the Key to Understanding Its Function The WatsonCrick Model Copyright 2009 Pearson Education, Inc. Figure 10.14 Copyright 2009 Pearson Education, Inc. Figure 10.14a Copyright 2009 Pearson Education, Inc. Figure 10.14b Copyright 2009 Pearson Education, Inc. Figure 10.14c Copyright 2009 Pearson Education, Inc. Figure 10.15 Copyright 2009 Pearson Education, Inc. Figure 10.16 Copyright 2009 Pearson Education, Inc. 10.8Alternative Forms of DNA Exist Copyright 2009 Pearson Education, Inc. Figure 10.17 Copyright 2009 Pearson Education, Inc. Figure 10.17a Copyright 2009 Pearson Education, Inc. Figure 10.17b Copyright 2009 Pearson Education, Inc. 10.9The Structure of RNA Is Chemically Similar to DNA, but Single Stranded Copyright 2009 Pearson Education, Inc. Table 10.4 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Absorption of Ultraviolet Light (UV) Sedimentation Behavior Copyright 2009 Pearson Education, Inc. Figure 10.18 Copyright 2009 Pearson Education, Inc. Figure 10.19 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Denaturation and Renaturation of Nucleic Acids Copyright 2009 Pearson Education, Inc. Figure 10.20 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Molecular Hybridization Copyright 2009 Pearson Education, Inc. Figure 10.21 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Fluorescent in situ Hybridization (FISH) Copyright 2009 Pearson Education, Inc. Figure 10.22 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Reassociation Kinetics and Repetitive DNA Copyright 2009 Pearson Education, Inc. Figure C/C 0 = 1/(1+kC 0 t) Copyright 2009 Pearson Education, Inc. Figure 10.24 Copyright 2009 Pearson Education, Inc. Figure 10.25 Copyright 2009 Pearson Education, Inc. Figure 10.26 Copyright 2009 Pearson Education, Inc Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Electrophoresis of Nucleic Acids Copyright 2009 Pearson Education, Inc. Figure 10.27