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Conference Program and Abstracts

The Annual Meeting of the Asian Society of Ichthyologists - ASI 2017

22 – 24 August 2017

Ho Chi Minh city, Viet Nam

Organizing Committee of ASI 2017

Organizer

Assoc. Prof. Hoang Duc Huy,

Vietnam National University – Ho Chi Minh City University of Science, Vietnam

Local Organizers

Dr. Pham Quynh Huong,


Ms. Tran Trong Ngan,


Mr. Pham Manh Hung,


Scientific Committee

Assoc. Prof. Wei-Jei Chen,

National Taiwan University, Taiwan

Elected president of the Asian Society of Ichthyologists (ASI)

Prof. Siti Azizah Mohd Nor,

School of Biological Sciences,

Universiti Sains Malaysia, Penang, Malaysia

Assoc. Prof. Yahui Zhao,

Chinese Academy of Sciences, Institute of Zoology, Beijing, China

Dr. Larry M. Page,

Dickinson Hall, Florida Museum of Natural History, Florida, USA

Assoc. Prof. Hoang Duc Huy,


Dr. Jacques Panfili,

IRD, UMR MARBEC, Ho Chi Minh City University of Science, Vietnam

Dr. Jean-Dominique Durand,

IRD, UMR MARBEC, Ho Chi Minh City University of Science, Vietnam

Welcome!

Asian Society of Ichthyologists (ASI) - Association of Asian College of

Fisheries Researchers - was established in 2014 in Penang, Malaysia.

Since its founding, ASI has drawn the attention and networking of world

academics through its annual conferences such as the 2014 Penang

Conference in Malaysia, 2015 Guilin in China and 2016 Taipei in Taiwan.

This year, the ASI 2017 is organized in Ho Chi Minh city, Vietnam on 22-24

August 2017 by Assoc. Prof. Hoang Duc Huy, University of Science - VNU

HCM. The conference is an opportunity for ichthyologists to meet, present

and publish new research results; Exchange ideas as well as professional

experience; Develop cooperative relationships across sectors such as

taxonomy, ecology, evolution, exploitation and protection of aquatic

resources, etc.

We wish you a successful participation with fun and enjoy the summer time

in Ho Chi Minh City!

Dr. Hoang Duc Huy

Assoc. Professor

Faculty of Biology and Biotechnology

University of Science - VNU HCM, Viet Nam

Contents

1 General Program

3 Map of the Conference location

5 Oral Presentation Program

13 Poster Presentation List

15 Keynote Speaker Communications - Abstracts

18 Oral Presentation - Abstracts

74 Poster Presentation - Abstracts

93 Participant List

Asian Society of Ichthyologists 2017

Page 1

General Program

DATE TIME PROGRAM

21 August 2017

17:30 - 19:00

Registration

Welcome and cocktail party at Building I, Floor G, University of Science,

HCM city

22 August 2017

7:00 - 8:00 Registration

at Hotel Equatorial, HCM city

8:00 - 8:10 Opening ceremory

at Hotel Equatorial, HCM city

8:10 - 8:40 Room Saigon I: Keynote speaker

communications

8:40 - 9:20

Room Saigon I: Session 1: Diversity,

Taxonomy and Phylogenetics

Room Saigon II: Session 2:

Ecology, Life History, Fisheries, Genetics and

Aquaculture

9:20 - 9:40 Coffee break and Poster session

9:40 - 11:20





Aquaculture

11:20 - 13:00 Lunch

13:00 - 15:00





Aquaculture


15:20 - 17:00





Aquaculture

17:30 - 21:00 Conference Dinner at Tau Saigon Floating

Restaurant 5 Nguyen Tat Thanh, District 4, HCM city


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DATE TIME PROGRAM

23 August 2017

8:00 - 9:40 Room Saigon I:

Session 1: Diversity, Taxonomy, and Phylogenetics


10:00 - 11:20 Room Saigon I:

Session 1: Diversity, Taxonomy and Phylogenetics

11:20 - 13:00 Lunch

13:00 - 14:20 Room Saigon I:



14:40 - 16:00 Room Saigon I:


16:00 - 16:30 Room Saigon I: Keynote speaker

communications

16:30 - 17:00 Closing ceremory

24 August 2017

8:00 - 17:00 Fieldtrip in Can Gio Biosphere Reserve and

Mekong Delta

22:00 - 2:00 Fieldtrip in Binh Dien fish market

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22 August 2017 (Tuesday) O

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Time Agenda

7:00 Registration

Room Saigon I

Moderator Assoc. Prof. Hoang Duc Huy, Dr. Pham Quynh Huong

Vietnam National University - Ho Chi Minh City University of Science, Vietnam

8:00 Opening ceremory

8:10

Keynote speaker communications

Dr. Tan Heok Hui National University of Singapore

Documenting Fish species from Sundaland

Room Saigon I Saigon II

Moderator Prof. Yahui Zhao

Institute of Zoology, Chinese Academy of Sciences

Prof. Zainal Abidin Muchlisin Syiah Kuala University

Session Session 1 Session 2

8:40

Dr. Barry Russell Northern Territory Museum

F.L. Castelnau's contribution to early Asian ichthyology

Mr. Javaid Ayub former Director Fisheries and wildlife

Study of Ichthyofauna of River Poonch, Azad Jammu and Kashmir Pakistan

9:00

Prof. Wei-Jen Chen Institute of Oceanography, National Taiwan

University Progress on the research of fish systematics

through the Tropical Deep-Sea Benthos program

Dr. Chunlong Liu Institute of Oceanology, Chinese Academy of

Sciences Dynamic conservation plan on species invasion

revealed by spatial and temporal changes in invasion risk

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9:20 Coffee break and Poster Session

9:40

Dr. Lawrence M. Page Florida Museum of Natural History

Taxonomic review of Nemacheilus (Cypriniformes: Nemacheilidae) in mainland Southeast Asia.

Dr. Shan Li Natural History Research Center, Shanghai

Natural History Museum Invasiveness screening of non-native freshwater fishes in the middle reach of the Yarlung Zangbo

River

10:00

Ms. Jinqing Huang Guangxi University,

College of Life Science and Technology A new cave loach (Cypriniformes: Nemacheilidae)

found in South China

Prof. Xu Li Southwest Forestry University

Survival Status Survey of the Rare and Endemic Schizothorax in Lugu Lake

10:20

Dr. Chaiwut Grudpan Department of Fisheries, Faculty of Agriculture,

Ubon Ratchathani University The new species of fishes (Pisces: Balitoridae and Mastacembelidae) from the Mekong Gorge Area,

Ubon Ratchathani, Thailand.

Dr. Amirrudin B. Ahmad School of Marine and Environmental Sciences

Diversity and species composition of fish community of Peninsular Malaysia small streams

10:40

Dr. Yumnam Lokeshwor Singh Department of Zoology, University of Science &

Technology Meghalaya Schisturid fish Diversity in the Eastern Himalayan

Region of India

Mr. Mohamad Aqmal Bin Mohd Naser Universiti Malaysia Terengganu (UMT)

Fish diversity and emergence of non-native species in rice agroecosystem in northern

Peninsular

11:00

Mr. Fan Li Department of Oceanography, National Sun Yat-

Sen University An undescribed genus and species of bitterling from

China (Teleostei: Cyprinidae: Acheilognathinae)

Mr. Kenny Chua Wei Jie National University of Singapore/Department

of Biological Sciences Functional diversity and vulnerability of freshwater

fishes in Sundaland: making fair comparisons across unevenly sampled ecoregions

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11:20 Lunch

Room Saigon I Saigon II

Moderator Dr. Lawrence M. Page

Florida Museum of Natural History Dr. Jacques Panfili

Institut de Recherche pour le Développement

Session Session 1 Session 2

13:00

Prof. Yahui ZHAO Institute of Zoology, Chinese Academy of

Sciences Beauty under Ground: Cavefishes in China

Dr. Tran Duc Hau Department of Zoology, Faculty of Biology,

Hanoi National University of Education Larval and juvenile ichthyofauna in estuaries of the

northern Vietnam

13:20

Prof. Wei Zhou Southwest Forestry University/Faculty of

Forestry A Review of Garra (Teleostei: Cypriniformes) from

West Yunnan, China with Description of a New Species

Dr. Dinh Minh Quang Can Tho University

Spatial variation of Gobiid species assemblage in Mudflat and Mangrove habitat along Coastlines in

the Mekong Delta

13:40

Prof. I-Shiung Chen Institute of Marine Biology, National Taiwan

Ocean University Two new freshwater gobiid species of Rhinogobius (Teleostei, Gobiidae) from Fujian Province, China

Dr. Abu Hena Mustafa Kamal Universiti Putra Malaysia

Composition and Diversity of Phytoplankton in Relation to Environmental Variables and

Seasonality in a Tropical Mangrove Estuary, Malaysia

14:00

Mr. Yang Ding Guangxi University,

College of Life Science and Technology Development and Application of Index of Biological Integrity (IBI) Based on Fish to Assess the River’s

Health in the Upper Reach of Lijiang River

Dr. Javad Ghasemzadeh Chabahar Maritime University- Faculty of

Marine Sciences- Iran Reproductive Biology and Histological Study of

Gonads of Keeled Mullet (Chelon carinata Valenciennes, 1836) in the Persian Gulf and Oman

Sea

14:20

Prof. E Zhang Institute of Hydrobiology, Chinese Academy of

Sciences Underestimated species diversity of the catfish

genus Liobagrus from mainland China

Prof. Jen-Chieh Shiao Institute of Oceanography, National Taiwan

University Application of isotopic analysis to reconstruct

habitat use of Japanese sea bass (Lateolabrax japonicas)

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14:40

Ms. Xue-Lin Song Institute of Hydrobiology, Chinese Academy of

Sciences Taxonomic revision of the Cyprinid genus

Onychostoma Günther, 1896 and its phylogeny

Ms. Iki Murase Graduate school of Fisheries and

Environmental Sciences, Nagasaki University Geographic variation in life history of ayu species


15:20

Ms. Nguyen Hoang Xuan Anh Biology Department, Hanoi National University

of Education A review of the sparid fish (Perciformes: Sparidae)

from the Tien Yen River, northern Vietnam

Dr. Matthew A Campbell University of California Santa Cruz

Adaptation to residency in rainbow trout (O. mykiss) above barriers to migration: Alternative

molecular pathways towards a predictable phenotype

15:40 Mr. Zachary Randall

Florida Museum of Natural History Freshwater Fishes of the Mae Khlong Drainage

Mr. Nur Fadli School of Biological Sciences

Population Genetics of Aerolate Grouper (Epinephelus aerolatus) in Indo-Malaya

Archipelago utilizing mitochondrial cytochrome c oxidase subunit I (COI) gene

16:00

Dr. Jarungjit Grudpan Department of Fisheries, Faculty of Agriculture,

Ubon Ratchathani University Two new species of the Cyprinid Fishes

(Cyprinidae: Hypsibarbus and Mystacoleucus) from the Southern part of Shan-Tenasserim

Geographical Region

Dr. Abdullah. A. Muhammadar Syiah Kuala University/ Aquaculture

Effect of Salinity Changing on Methionine Content in Tiger Grouper Juvenile (Epinephelus

fuscoguttatus)

16:20

Dr. Seah Ying Giat Universiti Malaysia Terengganu

Historical and Recent Records on Malaysian Croakers

Prof. Wazir Singh Lakra ICAR- Central Institute of Fisheries Education

Blue Revolution in India

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16:40

Mr. Lim Hong-Chiun Universiti Sains Malaysia

Evidence of Cryptic Diversity of Freshwater Halfbeak Hemirhamphodon pogonognathus in

Sundaland

Prof. Hongyu Ma Shantou University, China

Genetic Sex Differences in the Mud Crab (Scylla paramamosain): Evidence from Sex Specific SNPs, and Differently Expressed Genes and

lncRNAs

17:00 Poster Session

17:30 Conference Dinner at Tau Saigon Floating Restaurant

5 Nguyen Tat Thanh, District 4, HCM city

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23 August 2017 (Wednesday)

Time Agenda

Room Saigon I

Moderator Prof. Wei-Jen Chen

Institute of Oceanography, National Taiwan University

Session Session 1

8:00 Prof. Siti Azizah Mohd Nor Universiti Sains Malaysia

Exploring hidden diversity in the Dermogenys spp. through DNA barcoding

8:20 Mrs. Wahidah Mohd Arshaad

Marine Fishery Resources Development and Management Department DNA Barcoding of Ray in Malaysia and Viet Nam

8:40

Ms. Noorul Azliana Binti Jamaludin Southeast Asia Fisheries Development Center (SEAFDEC)/Marine Fishery Resources and

Management Department (MFRDMD) DNA Barcoding reveals targeted species of sharks in Malaysia and Vietnam

9:00 Prof. Md. Sagir Ahmed Department of Zoology

DNA barcoding of freshwater fishes of Bangladesh

9:20

Dr. Mao-Ying Lee Institute of Oceanography, National Taiwan University

Diversity Exploration and Taxonomy in Symphurine Tonguefishes (Symphurus: Cynoglossidae) from Papua New Guinea, western Pacific, by combing morphological and molecular evidences


10:00 Dr. Dang Thuy Binh

Institute for Biotechnology and Environment, Nha Trang University Species diversity and phylogeny of Anguilliformes in Vietnam

10:20

Mr. Agung Setia Batubara Syiah Kuala University, Doctoral Program in Mathematics and Sciences Application (DMAS),

Graduate Program Morphometric and Genetic Variations of Genus Barbonymus (Pisces, Cyprinidae) harvested from Aceh

Waters, Indonesia

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10:40 Dr. Nuzhat Shafi

Department of Zoology, University of Azad Jammu and Kashmir (UAJK) Phylogenetic Analysis (16sr RNA) of Rainbow trout of Azad Jammu and Kashmir, Pakistan

11:00

Dr. Nian-Hong Jang-Liaw Taipei Zoo

Phylogeography of Barbodes semifasciolatus complex (Teleostei, Cyprinidae) of Hainan Island and adjacent area

11:20 Dr. Hoang Duc Huy

University of Science, Vietnam National University, Ho Chi Minh city Stream fishes (Teleostei) from Central Highlands and South Central Coast of Vietnam

11:40 Lunch

Room Saigon I

Moderator Prof. Siti Azizah Mohd Nor Universiti Sains Malaysia

13:00

Dr. Hairong Luo South China Sea Institute of Oceanology,

Chinese Academy of Sciences Biodiversity and Biogeography of Flatfishes (Teleostei: Pleuronectiformes) living in Chinese Waters

Session Session 1

13:20

Mr. Jamsari Amirul Firdaus Jamaluddin Universiti Sains Malaysia

Insight into the phylogeny of Asian Spiny eel (Synbranchiformes: Mastacembelidae): implication for their diversity, taxonomy and biogeography

13:40

Mr. Ryohei Miki Nobeoka Marine Science Station, University of Miyazaki

Tropical marine fish aggregations along the eastern coast of Kyushu, Japan, northwestern Pacific Ocean, with an assessment of influence of the Kuroshio Current

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14:00

Ms. Hoang Kim Duyen Hanoi National University of Education

Variations of otolith morphology of larval and juvenile yellow sea bream Acanthopagrus latus (Houttuyn, 1782) collected from northern Vietnam


14:40

Dr. Jie Zhang Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese

Academy of Sciences Structure and function of corneal surface of mudskipper fishes

15:00

Dr. Keita Koeda National Museum of Marine Biology & Aquarium

Sweepers (Teleostei: Perciformes: Pempheridae) in the Ogasawara Islands, Japan, with an undescribed species of the genus Pempheris

15:20

Mr. Harutaka Hata United Graduate School of Agricultural Sciences, Kagoshima University

An undescribed species of the anchovy genus Stolephorus (Clupeiformes: Engraulidae) from Southeast Asia

15:40

Mr. Derita Yulianto Syiah Kuala University/ Doctoral Program in Mathematics and Sciences Application (DMAS)

Truss morphometric analysis of mullets (Mugillidae) harvested from Lambada waters, Aceh Besar district, Indonesia

Room Saigon I

Moderator Assoc. Prof. Hoang Duc Huy, Dr. Pham Quynh Huong

Vietnam National University - Ho Chi Minh City University of Science, Vietnam

16:00

Keynote speaker communications

Prof. Zainal Abidin Muchlisin Syiah Kuala University

Morphometric variations of the Groupers (Serranidae) harvested from Pulo Aceh waters, Aceh province, Indonesia

16:30 Closing ceremony

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Code Name Title PO

ST

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S1-1 Mr. Chu Hoang Nam Morphological description of larvae and juveniles of flatfish (Pleuronectiformes)

collected from Ka Long and Tien Yen estuary, Northern Viet Nam

S1-2 Mr. Pakorn

Tongboonkua

A new species of sinistral flatfish genus Chascanopsetta (Teleostei: Bothidae)

from Papua New Guinea, western Pacific Ocean

S1-3 Prof. Wei-Jen Chen The anatomy of the endocrine pancreas among teleosts: phylogenetic

implications

S1-4 Ms. Shih-Yu Wang Molecular Systematics of lizard fishes (Teleostei: Synodontidae): a preliminary

result based on two gene makers

S1-5

Dr. Mohammad

Sadegh Alavi-

Yeganeh

Identification and morphological comparison of Sillaginidae Species in Southern

Coast of Iran by using traditional morphologic characters and Geometric

statistics

S1-6 Mr. Thanh Huynh Kim Survey on the species composition of the Perciformes in different habitats in

Con Dao island, Vietnam

S1-7 Prof. Md. Sagir

Ahmed

Molecular characterization and phylogenetic relationship of freshwater barbs of

Bangladesh exploring Cytochrome Oxidase Subunit I (COI) gene

S1-8 Dr. Kang-Ning Shen Phylogenetic evolution of global marine angelfishes (Pomacanthidae): a

coordinated study by molecular phylogeny and otolith morphometry

S1-9 Ms. Thao Thi Bich Vo Identifying fish larvae by DNA barcoding approach at Con Dao Island, Vietnam

S1-10 Mrs. To Thuy Dung Asymmestry otoliths of Sillago sihama (Forsskal, 1775) in some areas of

Vietnam.

S2-1 Ms. Cheng, Ching-

Chun

Age composition and population mixing of Pacific bluefin tuna by using otoliths

annuli and isotope analysis

S2-2 Mr. Han Shang Jung Fast identification of Forbidden shark species by insulated isothermal PCR，

iiPCR technique

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S2-3 Mr. Feng Lin Comparisons on biological characteristics of Topmouth gudgeon of the invasion

and original areas in China

S2-4 Ms. Jhen Hsu The study of changes in size, age, sex ratio and otolith sampling methods of

Pacific Bluefin tuna (Thunnus orientalis) caught in the northwestern

S2-5 Prof. Hui Zhang

Comparative study the expression of calcium cycling genes in Bombay

duck (Harpadon nehereus) and beltfish (Trichiurus lepturus) with

different swimming activities

S2-6 Prof. Md. Sagir

Ahmed Bangladesh Barcode of Life (BdBOL)

S2-7 Prof. Yongshuang

Xiao

Population genetic structure of the rock bream; Oplegnathus fasciatus based on

the SSR

S2-8 Mr. Danial Hariz

Zainal Abidin

DNA Barcoding of the Japanese scad, Decapterus maruadsi (Temminck &

Schlegel, 1843) in South China Sea and neighbouring waters


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Keynote Speaker Communications

Abstracts


Page 16

Documenting Fish species from Sundaland

Tan Heok Hui

Lee Kong Chian Natural History Museum, National University of Singapore

Corresponding author: Tan Heok Hui ([email protected])

There is at present an estimated 3000+ species of freshwater fish recorded from

Southeast Asia, and this figure is expected to rise. This increase in species number can

be attributed to several factors: practise of modern species concepts, ability to use fresh

characters (e.g. colour), recognition of cryptic species using both morphology and

genetic clues, and exploration of neglected habitats. But, are new species that easy to

come by? Are special tools or skills required? Does the systematic research work end

there? What other potential areas of study are available? The speaker will draw upon

personal field experience and research work to shed some light upon the exploration

phase of freshwater field work, subsequent discovery of new fish species and follow-up

research work.


Page 17

Morphometric Variations of the Groupers (Serranidae) harvested

from Pulo Aceh waters, Aceh province, Indonesia

Zainal A. Muchlisin, Afriana Ramadhani, Muhammad Ali Sarong,

Agung Setia Batubara

Syiah Kuala University, Faculty of Marine and Fisheries, Kopelma Darussalam, Banda Aeh

23111, Indonesia

Corresponding author: Zainal A. Muchlisin ([email protected])

The objective of the present study was to examine the morphometric variations of the

groupers within the family of Serranidae harvested from Pulo Aceh waters, Aceh

Province, Indonesia. The sampling was conducted from May to September 2016 in two

landing sites i.e. Ulelhee, Banda Aceh City and Ujong Pancu village of Aceh Besar

District. The fish samples were catches by fishermen using line hand fishing and bottom

gillnet. Based on the initial survey showed that five species of Serranidae were

frequently caught by fishermen, namely; Plectropomus leopardus, P. laevis, P.

maculatus, Epinephelus fuscoguttatus and E. bleekeri. A total of 200 fish samples (40

sample of each species) were used in this study. A total of 14 truss morphometric

characters were measured using digital calipers. The study showed that three group of

Serranidae was successfully discriminated. The first group consisted P. leavis and P.

leopardus, the second group consisted E. bleekeri and E. fuscoguttatus, and the third

group was P. maculates, where the first and third groups were high morphological

similarity.


Page 18

Oral Presentation

Abstracts


O-S1 ǀ Page 19

F.L. Castelnau's contribution to early Asian ichthyology

Barry C Russell

Northern Territory Museum, GPO Box 4646, Darwin, NT 0801, Australia

Corresponding author: Barry C Russell ([email protected])

François Louis Nompar de Caumont Laporte, comte de Castelnau, also known as

Francis de Castelnau (1802-1880), was a widely traveled French explorer, naturalist

and diplomat in the 19th Century. In 1843-47 he led a major expedition to the Matto

Grosso, Brazil, and Peru, but following the 1848 Revolution in France he became a

diplomat and was successively appointed as French Consul at Salvador de Bahia,

Brazil (1848-55); the Cape of Good Hope, South Africa (1856-58); Bangkok, Siam

(1858-62); and Melbourne, Australia (1863-1877), where he remained until his death

in 1880. Castelnau was well known as an entomologist who, under the name François

Laporte, published more than 35 papers, mainly on beetles (Coleoptera). He was also

a prolific ichthyologist, who took advantage of his travels and various diplomatic

postings to collect and describe a total of 469 new species of fishes which he published

in 24 papers using the name Francis de Castelnau. While based in Bangkok, Castelnau

travelled widely in Southeast Asia and collected and painted fishes from places he

visited. In Batavia, he met the famous Dutch ichthyologist Pieter Bleeker, with whom

he corresponded and shared his notes and paintings, and who subsequently published

a series of papers on the fishes of Siam and Singapore, based largely on Castelnau’s

work. For nearly 150 years, Castelnau’s collections and notebooks were thought to be

lost, until recently uncovered in the Zoology Museum of the University of Liège

(ZMUL), Belgium. Castelnau’s fish collections in the ZMUL from his time in Bangkok

comprise about 250 dried mounted specimens, mainly from Siam and Singapore. His

illustrations are contained in a series of folios, and include paintings of more than 940

fishes from Singapore (616), Siam (181), Malacca (17), Batavia (15), Palembang (6),

Saigon (4), ‘China’ (2) and Banka (1). Castelnau’s paintings and specimens are

historically and scientifically important, and provide a source of verification and

identification for publications by Bleeker on the fishes of Siam and Singapore.


O-S1 ǀ Page 20

Progress on the research of fish systematics through the Tropical Deep-Sea

Benthos program

Wei-Jen Chen

Institute of Oceanography, National Taiwan University, No.1 Sec. 4 Roosevelt Rd.

Taipei 10617, Taiwan

Corresponding author: Wei-Jen Chen ([email protected])

March 22, 1976, the research vessel Vauban catches the "living fossil" mud lobster

Neoglyphea inopinata in deep water off Lubang Island, in the Philippines, exactly from

where the Albatross had collected the first specimen in 1908. This spectacular

rediscovery becomes the birthmark of the "Musorstom campaigns" which in 1999

became the Tropical Deep-Sea Benthos (TDSB) expeditions. With Neoglyphea came

a cornucopia of all kinds of benthic biota which Jacques Forest and Alain Crosnier, the

initiators of the program, farmed out to a worldwide network of specialists. Taiwanese

partners jointed this program since 2000 and the cooperative activities of both countries

became important thanks to the bilateral cooperation research project from MOST and

ANR entitled “Taiwan France marine diversity exploration and evolution of deep-sea

fauna (TFDeepEvo)” conducted between 2013 and 2016. In 2016, forty years later, the

TDSB expeditions have made over 5,000 dredge and trawls hauls for the biodiversity

explorations. Here, I present some progress on the research of fish systematics through

the TDSB. By combining our exploration strategy with some hypothesis-driven

studies, we provided a better access to the taxonomic knowledge of the deep-sea fishes,

especially with help of modern molecular tools, and better understandings of causes of

the pattern of diversity and evolution of the deep-sea fauna.


O-S1 ǀ Page 21

Taxonomic review of Nemacheilus (Cypriniformes: Nemacheilidae) in

mainland Southeast Asia

Lawrence M. Page and John M. Pfeiffer

Florida Museum of Natural History, University of Florida, Gainesville, Florida, 32611,

USA

Corresponding author: Lawrence M. Page ([email protected]), John M. Pfeiffer

([email protected])

Currently recognized species of Nemacheilus in mainland Southeast Asia are N.

arenicolus, N. banar, N. binotatus, N. cleopatra, N. longistriatus, N. maysae, N. ornatus,

N. pallidus, N. platiceps, N. paucimaculatus, N. selangoricus, and N. troglocataractus.

Analyses of morphological and molecular data (COI+RAG1) from recently collected

specimens from Thailand, Cambodia, and Malaysia indicate that N. pallidus is a junior

synonym of N. maysae, and an undescribed species of Nemacheilus occurs in the large

tributaries of the Mekong River in Thailand. The new species is small -- with a

maximum known standard length (SL) of 26.5 mm, has a color pattern of dusky bars

that cross the back and extend down the side of the body with no conspicuous black

spots, and modally 10 pectoral rays. Molecular data suggest that it is most closely

related to N. maysae, which reaches a much larger size – to 66.2 mm SL, has black

blotches along the side of the body and along the dorsal midline, a conspicuous black

spot on the anterior dorsal-fin rays, and modally 11-13 pectoral rays. Nemacheilus

maysae occurs throughout mainland Southeast Asia including the Mae Khlong, Chao

Phraya, Mekong, and many coastal drainages of peninsular Thailand and Malaysia. The

new species appears to be restricted to the Khorat Plataeu ecoregion of the Mekong

River Basin, and is known only from the Songkram and Mun River systems in Thailand.


O-S1 ǀ Page 22

A new cave loach (Cypriniformes: Nemacheilidae) found in South China

J.-Q. Huang, Z.-Q. Wu, Y.-H. Zhao

Guangxi University, College of Life Science and Technology, No. 100 Daxue East Road,

Nanning City, Guangxi Zhuang Autonomous Region, P.R. China

Corresponding author: Yahui Zhao ([email protected]), Zhiqiang Wu ([email protected])

A new cave-dwelling loach was found from Guangxi, China. It belongs to a new-

established genus Troglonectes. This new species can be distinguished from those blind

or eye-doted species in the genus including T. acridorsalis, T. barbatus, T. elonatus, T.

macrolepis, T. microphthalmus, T. translucens and T. duanensis by having functional

eyes. It is most similar to T. furcocaudalis from body shape and color pattern. However,

the new species differs to the latter by having 8-9 branched dorsal-fin rays (vs. 7), body

width (at anus) percent (%) of standard length 8.2±0.3 (vs. 5.0±1.0), distance between

anterior and posterior nostrils percent (%) of head length 2.0±0.0 (vs. 6.1±1.0), distance

between posterior nostril and eye percent (%) of head length 14.5±0.8 (vs. 10.0±1.9).

This new species is only found in a subterranean pool with diameter about 10 meters.

Interestingly, it has a sympatric distribution with several other cavefishes such as T.

translucens, Protocobitis typhlops and Sinocyclocheilus macrophthalmus.


O-S1 ǀ Page 23

The new species of fishes (Pisces: Balitoridae and Mastacembelidae) from

the Mekong Gorge Area, Ubon Ratchathani, Thailand

Chaiwut Grudpan

Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University,

Warin Chamrab, Ubon Ratchathani, Thailand 34190

Corresponding author: Chaiwut Grudpan ([email protected])

The Mekong gorge area is the uniqueness geographical pattern in the Mekong region

located along the Mekong mainstream through the sandstone table mountain bordering

Thailand and Lao PDR. Two new species of fishes founded from rocky rapids habitat

especially in the dry season. Schistura n.sp. has complete lateral line scales with the

most attractive characteristic can be easily distinguished from others in the same genus

with 3 longitudinal stripes running from the tip of snout to the caudal fin base at mid-

dorsal and mid-lateral of body but this characters shared similarity to others genera,

Nemacheilus binotatus and Ambastaia nigrolineata.

Macrognathus n.sp. is the second new species founded from this area. Share the color

patterns of dorsal and anal fin base with ocellate marking included the low number of

rostral tooth plates with M. siamensis compared with others in the same genus (less than

14 vs. 14 or more). The new species can be distinguished with the congener by the

longer length and shape of snout, the caudal fin strongly confluent with dorsal and anal

fin compare with the separated caudal fin with dorsal and anal fin.


O-S1 ǀ Page 24

Schisturid fish Diversity in the Eastern Himalayan Region of India

Yumnam Lokeshwor Singh

Department of Zoology, University of Science & Technology Meghalaya, Techno City,

Kling Road, Baridua, Ri-Bhoi District, Meghalaya, India -- 793101

Corresponding author: Yumnam Lokeshwor Singh ([email protected])

The Eastern Himalayan Region of India is drained by four major drainages viz. the

Ganga-Brahmaputra, the Barak-Surma-Meghna, the Chindwin-Irrawaddy and the

Kaladan/ Kolodyne. These drainages are the repository of nemacheilid loaches, holding

more than 53 nemacheilid species under five genera viz. Aborichthys, Acanthocobitis,

Neonoemacheilus, Physoschistura and Schistura, representing 16% of total fish species

of the region. Out of the total nemacheilid species of the region, the present study

recognises 34 species under genus Schistura from different drainages of the northeastern

India; of which 11 schisturid species are described recently viz. Schistura aizawlensis,

S. fasciata, S. ferruginea, S. koladynensis, S. paucireticulata, S. porocephala, S.

liyaiensis, S. maculosa, S. nebeshwari, S. phamhringi and S. scyphovectata. The Barak-

Surma-Meghna drainage harboured with 11 species; the Brahmaputra drainage with 10;

the Chindwin-Irrawaddy drainages of the region with 9 species; and the Koladyne

drainage with 4 species (described recently) only. Low species representation from the

Koladyne drainage might be inability to explore the entire stretch of the river basin,

inaccessible to reach various areas due to hostile geo-morphological features, and

various river and land laws for each and every tribe inhabited along the river basin that

prohibit the entry of non-local people for exploration. Schisturid species described

recently to the world are Schistura koladynensis, S. porocephala, S. nebeshwari, and S.

scyphovectata. High degree of endemism is reported from the upper reaches of the river

basin of the region. Of the 11 species reported from the Barak-Surma-Meghna drainage

of the region, 6 species viz., Schistura aizawlensis, S. fasciata, S, ferruginea, S.

liyaiensis, S. maculosa and S. paucireticulata, are described recently to the world. Six

new Schistura, two each from Arunachal Pradesh, Meghalaya and Nagaland, have been

collected recently from Brahmaputra drainage of northeastern India. Out of 9 reported

schisturids from the Chindwin-Irrawaddy drainage of the region, a new Schistura has

been collected from Duta stream of Manipur, India. As per IUCN 2010 report, the region

has high species richness, endemism, and threats. In view of the prevalent threats to the

freshwater biodiversity of the region, proper exploration and evaluation of the

ichthyofaunal resources is essential for taking up conservational measures for future

generation.


O-S1 ǀ Page 25

An undescribed genus and species of bitterling from China

(Teleostei: Cyprinidae: Acheilognathinae)

Fan Li, Te-yu Liao, Ryoichi Arai, Liangjie Zhao

Department of Oceanography, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan, R. O. China.

Corresponding author: Fan Li ([email protected])

An undescribed genus and species from a tributary of the Yangtze River, in Chongqing,

China, will be presented. Phylogenetic analysis of one mitochondrial gene and six

nuclear genes places the new genus as a sister group of the unnamed (sensu Chang et

al. 2014) and Rhodeus clades, supporting the establishment of a new genus.


O-S1 ǀ Page 26

Beauty under Ground: Cavefishes in China

Yahui Zhao1, Danté Fenolio2, Daphne Soares3, Andrew Gluesenkamp2, Matthew

Niemiller4, Prosanta Chakrabarty5

1 Institute of Zoology, Chinese Academy of Sciences, No.1-5 Beichen Xilu Road,

Chaoyang District, Beijing 100101, P. R. China 2 Conservation and Research, San Antonio Zoo

3 New Jersey Institute of Technology 4 Illinois Natural History Museum

5 Louisiana State University Museum of Natural Science

Corresponding author: Yahui Zhao ([email protected])

Cavefishes, or hypogean fishes, are a distinctive group of fishes restricted to

subterranean environments for at least for part of their lives. Based on their

troglomorphism, which includes morphological adaptations to the cave environment

such as the reduction or loss of eyes and pigmentation, cavefishes can be divided to two

types: stygobites (troglomorphic) and stygophiles (non-troglomorphic). China is home

to the highest number of cavefish species in the world, with more than 70 stygobitic

species out of about 141 cavefish species. As our fieldwork continues, more species are

being found and described. All of these cavefish species are endemic to China. With the

exception of Onychostoma macrolepis in north China, all species are found in southwest

China, mostly in the karst environment of the Yunnan-Guizhou Plateau.

Sinocyclocheilus, the largest cavefish genus, has experienced rapid evolution and

diversification. Species from this genus possess horns and humpbacks (i.e. horn-like

structures and hyper development of a dorsal protuberance similar to a humpback),

probably resulting from parallel evolution. Sympatric distribution of Chinese cavefishes

is common, and sometimes several species are found in the same cave or subterranean

river. Caves and karsts are very fragile, balanced habitats, and cavefish species are

extremely sensitive to environmental change. Threats from rapid economic growth have

increased the need for conservation efforts for cave-dwelling communities in recent

decades. Conservation of Chinese cavefishes is an urgent issue.

The Chinese Cavefish Working Group is a collaborative team composed by researchers

from China and the United States. The primary goal is to document the distribution and

diversity of Chinese cavefishes and to gather information on their status and threats to

wild populations. This may be used to make sound conservation and management

decisions. In addition to these scientific goals, the CCWG seeks to train the next

generation of Chinese cave biologists so that they may continue to expand our

understanding of cavefishes in China. Research efforts are not limited to surveys and

collection but also include taxonomy, systematics, biomechanics, ecology, population

biology, and conservation science.


O-S1 ǀ Page 27

A Review of Garra (Teleostei: Cypriniformes) from West Yunnan, China

with Description of a New Species

Chao Sun1, Xu Li1, Wei Zhou1, Fenglian Li2

1 Faculty of Forestry, Southwest Forestry University, 300 Bailongsi Road, Kunming

650224, Yunnan, China, +86 (0)871 63862458 2 Wildlife Conservation Society China Program, Room2-1101, Tower2, Ronghua Shijia,

No. 29 Xiaoyingbeilu, Chaoyang District, Beijing 100101, China, +86 (0)10 8486-7735

Corresponding author: Wei Zhou ([email protected])

Species of the genus Garra (Cypriniformes: Cyprinidae) from the Irrawaddy River and

Salween River basins in west Yunnan, China are difficult to identify and their taxonomy

remains problematic. The dorsal tip of the snout and the frontal part of the head in each

species varies greatly. The snout in some species is smooth and not specialized; others

have a conspicuous, deeply notched secondary rostrum (a rostral projection). Thus, we

conducted a systematic review to identify this group using a combination of molecular

and morphological approaches. The results showed that 15 species of Garra did not

cluster as a monophyletic. Garra imberba and G. nujiangensis as a sister group form an

independent clade (A1) and are distanced from the rest of the Garra species, which are

clustered in another independent clade (B) in the phylogenetic tree. According to the

combination of morphological characters of the proboscis and rostrum, the snout shape

of Garra from western Yunnan Province, China can be divided into 4 types: (1) smooth

snout; (2) entire proboscis; (3) bi-lobed proboscis; and (4) tri-lobed proboscis. The types

of snout shapes in Garra are consistent with the topological structure of the

phylogenetic tree. It could be further deduced that the evolutionary direction of the

proboscis in Garra was from no proboscis to a simple proboscis, to an entire proboscis,

to bi-lobed and tri-lobed proboscises, separately. In addition, G. surgifrons, a new

species from Longchuan-jiang, an upper tributary of the Irrawaddy River in western

Yunnan is described here. Two specimens of G. surgifrons clustered into one distinct

clade within clade B in the phylogenetic tree. Garra surgifrons can be distinguished

from all other congeners occurring in China and Southeast Asia by a combination of the

following characters: having a rostral projection and a tri-lobed proboscis; both dorsal

and caudal fins without black mark; the proboscis short and broad, a roughly quadrate

shape from the dorsal view with a poorly developed and slightly protuberant middle

lobe whose front tip is not suspended from the lateral view and the width of the middle

lobe almost equal to the width of the lateral lobes; posterior margin of mental adhesive

disc extending close to or reaching vertical through the central line of the eye and never

reaching the posterior margin of the eye; two pairs of barbels; 16 circumpeduncular

scales; and 32–34 lateral line scales.


O-S1 ǀ Page 28

Two new freshwater gobiid species of Rhinogobius (Teleostei, Gobiidae)

from Fujian Province, China

I-Shiung Chen1, 2

1 Institute of Marine Biology, National Taiwan Ocean University, Jhongjheng, Keelung

20224, Taiwan 2 National Museum of Marine Science and Technology, Jhongjheng, Keelung 20248,

Taiwan

Corresponding author: I-Shiung Chen ([email protected])

Two new species of freshwater gobiid fish of genus Rhnogobius Gill, 1859, were

collected from the two different drainages (both Jangshi and Dongshi basins) of

southern region of Fujian Province, China. In the Fujian province, China, one fluvial

species, R. xianshuiensis, was firstly described from the upper tributary of the Mulan

River basin by Chen et al., 2009b. Two more species were described from the upper

tributaries of Hanjiang basin including both R. changtinensis and R. ponkouensis by

Huang & Chen, 2007. The fourth species was described from the hillstream of Minjiang

basin, as R. reticulatus Li & Zhong, 2007. Later on, another endemic species, R.

longyanensis Chen et al., 2008 was described from the Julongjiang basin, middle region

of the province. Two species including both R. rubrolineatus Chen & Miller, 2008 and

R. sagittus Chen & Miller, 2008 from the Minjiang basin. Both new species can be well

distinguished from other congeners by their specific patterns of coloration and meristic

features: and they belong to the non-diadromous, fluvial species with vertebral count 26

& 27. A diagnostic key to all valid species from Fujian Province, China would be

provided and comparative studies of related species would also be discussed.


O-S1 ǀ Page 29

Development and Application of Index of Biological Integrity (IBI)

Based on Fish to Assess the River’s Health in the Upper Reach of Lijiang

River

Yang Ding, Zhiqiang Wu, Zhaojun Zhu

Guangxi University, College of Life Science and Technology, No.100 Daxue East Road, Nanning City, Guangxi Zhuang Autonomous Region, P.R. China

Corresponding author: Zhiqiang Wu ([email protected])

Four samplings for the biodiversity of fish species were carried out quarterly during the

period from April 2014 to January 2015. Referring historical data, the indicator system

for river health assessment in the middle and lower reaches of Lijiang River was

established, and the current river health was evaluated for the middle- and lower-reach

of the Lijiang River. The main results of this study are as follows.

1. There were 10161 fish samples collected in the research area, and they were identified

to be 74 species, and fell into 5 orders, 15 families and 55 genera, respectively. The

major orders were Cypriniformes, Siluriformes, Cyprinodontiformes,

Synbgranchiformes, and Perciformes. Of all the species, Cypriniformes, including 3

families and 50 species, accounted for 67.57%, Perciformes, including 7 families and

14 species, accounted for 18.92%, Siluriformes, including 3 families and 8 species,

accounted for 10.81%, Cyprinodontiformes, including 1 families and 1 species,

accounted for 1.35%, and Synbgranchiformes, including 1 families and 1 species,

accounted for 1.35%.

2. Within the biodiversity, the peak value of Dma (7.98) appeared in summer, the peak

values of He' appeared in summer (3.24) and autumn (3.13), and the peak values of Je'

appeared in summer (0.79) and autumn (0.80). Meanwhile, the λ value was rather low,

being 0.06 in summer. Contrary to their counterparts in summer, the values of Dma、

He'、Je' reached their lowest level in spring, and the value of λ in spring reached the

highest level among the value variations in the four season. This indicates that the fish

biodiversity is high and the fish species is rich in summer, while the biodiversity is lower

and the fish species is poorer in spring. No quite difference was found between the

indexes and the biodiversity did not vary substantially in autumn and winter, indicating

that the seasonal change of the fish species is inconspicuous in these seasons. Besides,

the dominance index λ in spring was highest compared to other seasons. Zacco platypus

and carassius were observed to be in the lead in the number of individual, with the

percentages of 12.04% and 11.61%, respectively, of the total number of individual in

spring.

3. According to the results of the dimensionless sequencing graph of fish communities,

the sampling sites were relatively convergent, and intersected each other, except for the

Pingle county dissociating outside. The results of the ANOSIM suggest that the

structure of fish community in Pingle county varies from other sampling sites, as 39

species were collected, counting for 52.70% of the total number of species, where the

sharpbelly and hemiculterella wui predominated in number of species. Besides, the


O-S1 ǀ Page 30

results of NMDS indicate that the sampling sites in four seasons mingled with each

other, gathering together, and there was no significant seasonal-variation to be observed

(P>0.05).

4. Based on the level of order in the study of the composition of fish fauna, the degree

of presence at the level of order could not determine degree of presence at the level of

family. Meanwhile, on the level of family, the degree of presence of Cyprinidae fauna,

which appeared to be in lead in the number of genus, was low, whereas the degrees of

presence of Odontobutidae fauna and Channidae fauna, which appeared to be less in the

number of genus, were relatively high, indicating that the sequencing of absolute

number and sequencing of degree of presence vary to a large extent. However, on the

level of genus, if sequenced by degree of presence, Cyprinidae accounted for 9 in the

top 10, though each absolute number of the individual of these genera were less than 2,

and the degree of presence of Rhinogobius fauna, which was in the lead of the absolute

number, was merely 6.6%, indicating that the differentiated degree of fishes in the

Lijiang River is low, and the diversity is poor on the species level. Based on the above

analysis shows that the demonstrated the weak correlation between the two levels, and

the distinct difference between the analytical approach of sequencing degree of fauna

presence and the analytical approach of absolute number sequencing.

5. There were 22 initial indexes selected in this study, divided into 5 project levels

including species composition and richness, trophic structure, alien invasive species and

their health condition, fertility guild, and tolerance. 7 evaluation indexes were selected,

including the amount of fish species, the percentage the number of Cyprinidae species

accounted for the number of total species, the percentage the number of Gobioninae

species accounted for the number of total species, the percentage the number of

Cultrinae species accounted for the number of total species, the percentage the number

of omnivorous species accounted for the number of total species, the percentage the

number of sensitive species accounted for the number of total species, the percentage

the number of tolerant species accounted for the number of total species. The indicator

system for the index of biological integrity (F-IBI) was then established for the fish

species in the middle- and lower-reach of Lijiang River. A segmented analysis was

carried out for the Lijiang River, and the results suggest that the number of each fish

species in each segment of Lijiang River, merely scoring 1 in average, was generally

lower than the number of historical data. This revealed a sharp decrease of the fish

species in Lijiang River. In each segment, as in the middle- and lower-reach of the

Lijiang River, the IBI scores were all above 40, indicating an average- and above-the-

average level, and that no river reach is poor, or of none, in the number of fish.


O-S1 ǀ Page 31

Underestimated species diversity of the catfish genus Liobagrus from

mainland China

E Zhang

Institute of Hydrobiology, Chinese Academy of Sciences.

Corresponding author: E Zhang ([email protected])

We investigate the phylogenetic relationships of genus Liobagrus in mainland of China

using 1704 aligned base pairs of DNA sequence data from the Cyt b and CO1 genes of

the mitochondrial genome. A total of 584 sequences were amplified from 303

specimens. Specimens were collected from the Chang-Jiang basin, Zhu-Jiang basin,

Min-Jiang basin and Qiantang-Jiang basin. Maximum likelihood and Bayesian analysis

of the data indicate that there are many unknown species of Liobagrus in China. We are

far from elucidating the true diversity of Liobagrus.


O-S1 ǀ Page 32

Taxonomic revision of the Cyprinid genus Onychostoma Günther, 1896 and

its phylogeny

Xue-Lin Song

Institute of Hydrobiology, Chinese Academy of Sciences.

Corresponding author: Xue-Lin Song ([email protected])

The Asian-endemic genus Onychostoma includes a total of 23 valid species. The

monophyly of this genus, and the taxonomic position of some species were in doubt

recent years. We amplified two mtgenes cyt b, CO1 and a nuclear gene RAG2 and tried

to resolve these problems. The results showed species of Onychostoma interlaced with

Acrossocheilus, they formed a monophyletic clade, together with Folifer brevifilis.


O-S1 ǀ Page 33

A review of the sparid fish (Perciformes: Sparidae) from the Tien Yen River,

Northern Vietnam

Nguyen Hoang Xuan Anh

Biology Department, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay,

Hanoi

Corresponding author: Nguyen Hoang Xuan Anh ([email protected])

The family Sparidae consists of 38 genera and 155 species, mainly distributed in tropical

and temperate coastal waters. In Vietnam, this family consists of 3 genera, and 4 species,

in which, the number of species of the genus Acanthopagrusare dominant, and they

have high commercial value in fisheries. In the Tien Yen River, there are 4 sparid

species (i.e., Acanthopagrus latus, A. berda, Parargyrops edita and Rhabdosargus

sarba) that could be collected in the estuarine environment. Collections of sparid fish

from the Tien Yen River, northern Vietnam could be identified to 7 species. Of which,

2 were unknown species (Acanthopagrus sp. and Sparus sp.), and 2 were new records

(i.e., Acanthopagrusschlegeilii and Pagrus major) for the research area. The genera

Pagrus and Sparus were the first finding for Vietnam. Morphology of 7 species based

on 67 adult specimens from the Tien Yen River was also given. In addition, the present

study provided comparisons in morphological features of identified species to the above

2 unknown species.


O-S1 ǀ Page 34

Freshwater Fishes of the Mae Khlong Drainage

Zachary S. Randall, Lawrence M. Page

Florida Museum of Natural History. 1659 Museum Road, Gainesville, FL 32609

Corresponding author: Zachary S. Randall ([email protected])

The Mae Khlong drainage contains a rich abundance of freshwater fishes whose

diversity has gone undocumented. The Mae Khlong is one of four major river systems

(Mekong, Chao Phraya, and Salween) which drain the fresh waters of Thailand. The

Mae Khlong originates in western Thailand in the mountains of Kanchanaburi Province

and drains through Ratchaburi and Samut Sakhon provinces to the Gulf of Thailand.

Based on vouchered museum specimens from 109 collection events, the Mae Khlong

drainage consists of a large diversity of freshwater fishes representing 10 orders, 28

families, 89 genera, and 134 species. Institutional collections likely represent an

underestimate for the total number of species that occur in the drainage, including

several yet-to-be described species. At most of the collection sites, photographs of live

fishes were taken to document species diversity and variation that until now were

without high resolution images. The images give the best representation for natural

coloration patterns since coloration and skin transparency of a specimen are lost when

the specimen is preserved in ethanol. Live color photographs are extremely valuable for

field identifications and documenting natural coloration in fishes. Over the past years,

we have taken live images of around 74 genera and 90 species from the Mae Khlong

drainage.


O-S1 ǀ Page 35

Two new species of the Cyprinid Fishes (Cyprinidae: Hypsibarbus and

Mystacoleucus) from the Southern part of

Shan-Tenasserim Geographical Region

Jarungjit Grudpan

Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University,

Warin Chamrab, Ubon Ratchathani, Thailand 34190

Corresponding author: Jarungjit Grudpan ([email protected])

The Kra Buri River or Pak Chan River located at the Southern part of Shan-Tenasserim

Geographical Region called the Kra Isthmus of Malay peninsula on the bordered

between Thailand; Ranong Province and Myanmar; Tenasserim State. Two species of

the Cyprinid fishes found in the correlation with the congener from the northern part of

the same geographical region. Hypsibarbus n.sp. shared the more number of vertebrae

with H. salweenensis compare with others (37 and 38 VS less than 36). The new species

can be easily distinguished with H.salweenensis by the bright yellowish body and fins

VS the silver gray with hyaline fins.

The second, Mystacoleucus n.sp. is similar to M. argenteus by lowest number of

branched anal fin rays compared to others (6 VS 8-10). This species can be distinguished

with the congener by the more slender shape of dorsal fin spine and the color pattern of

fin by hyaline fins with black marginal tip at dorsal and reddish tips of caudal fin VS

the yellowish caudal fin and pelvic fins with the bright orange dorsal fin with black

marginal.


O-S1 ǀ Page 36

Historical and Recent Records on Malaysian Croakers

Ying Giat Seah1,2, Norhafiz Hanafi1, Abd Ghaffar Mazlan1, Li Lian Wong3, Ning

Labbish Chao4

1 School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu (UMT),

21030 Kuala Nerus, Malaysia 2 Fish Division, South China Sea Repository and Reference Center, INOS, UMT, 21030

Kuala Nerus, Malaysia 3 Institute of Tropical Aquaculture, UMT, 21030 Kuala Nerus, Malaysia

4 National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng,

Pingtung, 944, Taiwan, ROC

Corresponding author: Ying Giat Seah ([email protected])

The croakers (family Sciaenidae) are one of the popular food fishes, salted-dried fishes

and surimi seafood resources in Malaysia. This monophyletic family is having a

cosmopolitan distribution throughout the tropical continental shelf waters except few

species living solely in freshwater. With approximately 291 valid croaker species, there

are 32 species were reported occur in Malaysian waters, however, Malaysian literatures

only clearly documented 19 species. Previous study focusing on Peninsular Malaysia

was conducted using comparative morphology and partial mitochondrial 16S rRNA

gene sequences; managed to identify 21 species including one new species, one new

record and four species were deposited in Malaysia for the first time. In this recent

scientific exploration on East Malaysia in 2017, preliminary results suggested that a

total of 22 species were confirmed consisting one species firstly deposited in Malaysia,

with several possible new species still await confirmation. Based on the historical

records and four years of collected specimen studies, 26 species were verified present

in Malaysian waters whereas eight nominal species still not encountered yet and few

undescribed species still likely to be discovered. The marine biodiversity of Malaysia is

still not fully explored and further taxonomic studies are really necessary.


O-S1 ǀ Page 37

Evidence of Cryptic Diversity of Freshwater

Halfbeak Hemirhamphodon pogonognathus in Sundaland River Basins

Lim Hong-Chiun1, 2, SitiAzizah M.N.1

1 School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia 2 School of Biological Sciences, Faculty of Integrative Sciences and Technology, Quest

International University Perak, Jalan Raja Permaisuri Bainun, 30250 Ipoh, Perak, Malaysia

Corresponding author: Lim Hong-Chiun ([email protected])

Population level assessment and phylogeography of H. pogonognathus was conducted

with a combination of both mtDNA (cyt b region) and nDNA markers (Single Copy

Nucleotide Polymorphism (SCNP) markers, Hp5 and Hp54) to assess the genetic

variability, population structuring, historical demography as well as attempt to discover

hidden diversity. A total of twenty-five populations were included in the assessments.

The results revealed high population structure indicating that most of the populations

contribute to the total gene pool. In addition, the findings highlighted the presence of

hidden diversity or cryptic species which is agreement with the initial hypothesis of new

species discovery. The historical demographic analyses revealed that most of the

populations had experienced rapid population size reduction. Therefore, each of the

populations should be treated as a separate management unit in the context of

conservation. On the other hand, the geological history such as the cyclical glaciation

events during the Pleistocene epoch coupled with the Paleo-drainage rearrangements

have greatly influenced the genetic diversity of the H. pogonognathus populations.

Overall, a more integrated investigation framework needs to be carried out to resolve

the cryptic diversity issue, and conservation strategies and management plans should be

implemented on each of the populations for long term population sustainability and

conservation of species.


O-S1 ǀ Page 38

Exploring hidden diversity in the Dermogenys spp. through DNA barcoding

Nurul Farhana Samsudin1, Muchlisin Zainal Abidin2, Duong Thuy Yen3, Suwat

Tanyaros4, Larry Page5, Yahui Zhao6, Mark de Bruyn7, Siti Azizah Mohd Nor1

1 School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang,

Malaysia 2 Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, 23111, Indonesia

3 College of Aquaculture and Fisheries, Cantho University, Cantho, Vietnam 4 Faculty of Science and Fisheries Technology, Rajamangala University of Technology

Srivijaya Trang campus Sikao, 92150 Trang, Thailand 5 Florida Museum of Natural History, Gainesville 32611, Florida

6 Institute of Zoology, Chinese Academy of Sciences, Chaoyang District 100101, Beijing 7 School of Biological, Earth and Environmental Sciences, University of New South Wales,

Sydney, Australia

Corresponding author: Siti Azizah Mohd Nor ([email protected])

A DNA barcoding study was conducted on the halfbeak, genus Dermogenys to resolve

its taxonomic status. A total of 290 samples of Dermogenys spp. from 33 localities of

freshwater and brackish drainages of Malaysia, Sumatra, Thailand and Vietnam were

amplified based on the mitochondrial cytochrome oxidase c subunit I (COI) gene. A

contiguous fragment length of 651 base pairs successfully differentiated four presumed

species (D. collettei, D. siamensis, D. sumatrana and Dermogenys sp.) and two

geographical groups of D. bispina; Kudat and Sandakan D. bispina. The putative

Dermogenys sp. was sampled in sympatry with D. collettei in three populations. This

study proved the efficacy of DNA barcoding technique to differentiate Dermogenys

species and its potential in new and hybrid species discoveries.


O-S1 ǀ Page 39

DNA Barcoding of Ray in Malaysia and Viet Nam

Wahidah Mohd Arshaad, Noorul Azliana Jamaludin and Ahmad Ali

Marine Fishery Resources Development and Management Department, Taman Perikanan

Chendering, 21080 Kuala Terengganu, Terengganu, Malaysia

Corresponding author: Wahidah Mohd Arshaad ([email protected])

The Southeast Asian Region has a rich fauna of rays. At least 148 species of rays and

skates were inhibiting this region from freshwater to deep ocean. In Southeast Asian

Region, Indonesia recorded the highest number of rays with 106 species and follow by

Malaysia, recorded 84 species. An accurate identification of ray species is one of the

most difficult tasks especially for look-alike species. We used molecular approach,

Cytochrome oxidase subunit I (COI) sequencing (~652bp) also known as DNA

barcoding to identify ray fishes sampled from Malaysia (Pahang, Terengganu, Perak,

Sabah and Sarawak) and Vung Tau, Viet Nam. DNA information for species of

elasmobranch (cartilaginous fish in the class Chondrichthyes, including sharks

(Selachii) and the rays, skates and sawfish (Batoidae) is accumulating. Total number

14,284 specimens with barcodes of elasmobranch COI sequences were lodged on

BOLD (Barcode of Life Data System) to date involves of 899 species collected from 47

countries worldwide. At least 35 numbers of rays species has been identified. The

phylogenetic relationships among these 41 species were review.


O-S1 ǀ Page 40

DNA Barcoding reveals targeted species of sharks in Malaysia and Vietnam

Noorul-Azliana J., Wahidah M.A., Ahmad A.

Southeast Asia Fisheries Development Center (SEAFDEC)/Marine Fishery Resources

Development and Management Department (MFRDMD), Kuala Terengganu, Terengganu,

Malaysia

Corresponding author: Wahidah Mohd Arshaad ([email protected]),

Dr. Ahmad Ali ([email protected])

Species identification is the first basic steps in phylogenetic, biodiversity monitoring

and conservation. DNA Barcoding has been proposed to provide an update on the

composition of shark in the Malaysia and Vietnam waters. In this study, species

identification system through DNA Barcoding approach was applied to 121 specimens

inferred by mitochondrial COI gene. Phylogenetic signals were also apparent in this

dataset.

The samples were collected from anal fin of sharks at the landing port from Malaysia

namely; Kuantan, Sandakan, Kota Kinabalu, Bagan Panchor, Mukah and Vung Tau,

Vietnam. From all identified specimens with approximately 660bp region of the

mitochondrial COI gene were provided with chondrichthyan sequence and could be

matched to reference specimens in DNA barcode database and were able to identified.

Of these, 34 species of sharks were detected. The most abundant species was

Rhizoprionodon acutus (13/121-10.7%). The fisheries managers can use this DNA

Barcoding to gather data since the lack of basic information for many sharks species

due to inaccurate identification of many chondrichthyan species in this region.


O-S1 ǀ Page 41

DNA Barcoding of Freshwater Fishes of Bangladesh

Md. Sagir Ahmed, Luthfun Nahar, Sabrina Rahman Dina, Nusrat Jahan Sanzida,

J.B.M. Aysha Akter and Nafisa Nawal Islam

Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh

Corresponding author: Md. Sagir Ahmed ([email protected])

The rapid increase of human population and consequent intensification of agricultural,

industrial and infrastructural activities along with deficient management have led to the

destruction of habitat, ecosystem and biodiversity of the country. Over fishing, use of

destructive fishing gears, catch of spawnner and under size fishes are the main causes

of loss fish diversity and production in the country. The country has already lost more

than a dozen vertebrate fauna during the last century. The lack of proper database on

our biodiversity is one of the greatest impediments for utilization and safeguarding of

our interests. The country now needs to maintain the genetic identity as well as integrity

of species in their natural habitats. This study represents the first comprehensive

molecular assessment of freshwater fishes from Bangladesh. We analysed cytochrome

c oxidase I (COI) gene sequences for 78% of the species mentioned in the current

Bangladesh Red List. Barcodes were obtained from 350 specimens, representing 195

species of freshwater fish belongs to 12 Orders and 57 Families. The average Kimura

two-parameter (K2P) distances within-species, genera, families, and orders were 0.32%,

15.83%, 19.14%, and 25.06%, respectively. DNA barcode discriminated congeneric

species without any confusion and some new cryptic species have been explored. This

is the first effort to compile a reference library of DNA barcodes that provides species

level identifications for freshwater fishes of Bangladesh. The study strongly validated

the efficiency of COI as an ideal marker for DNA barcoding of Bangladesh freshwater

fishes.


O-S1 ǀ Page 42

Diversity Exploration and Taxonomy in Symphurine Tonguefishes

(Symphurus: Cynoglossidae) from Papua New Guinea, western Pacific,

by combing morphological and molecular evidences

Mao-Ying Lee, Wei-Jen Chen

Institute of Oceanography, National Taiwan University, No.1, Sec. 4, Roosevelt Road,



Symphurine tonguefishes belong to the genus Symphurus. They are small-sized, left-

sided flatfishes. The species of this genus are distributed at tropical to temperate seas

around the world; most of them are deep-sea and occur at depths between 200 and 1500

meters. Symphurus includes about 80 valid species; among them, 30 species are found

in the tropical Indo-West Pacific. Although this genus displays great species diversity,

no studies so far documented the presence of any recognized species of Symphurus

around waters off Papua New Guinea. During several biodiversity expeditions in

western Pacific conducted under the Tropical Deep-Sea Benthos program and the

cooperation project between Taiwan and France, TFDeepEvo, we collected several

specimens from northern and eastern Papua New Guinea that can be further diagnosed

as Symphurus. After the detailed morphological comparisons with, for example, the

meristic and morphometric characters between examined specimens and type

specimens of other nominal species, we revealed that at least 11 species, including six

possibly new species could be sorted out from the collected specimens. Molecular

approaches have been regularly used to highlight the potential issues of cryptic diversity

of the nominal species; they provided also great help in recognizing the species with

accuracy. Our study demonstrated that the combined morphological and molecular

approaches are essential to complete a comprehensive taxonomic revision of

symphurine tonguefishes from Papua New Guinea.


O-S1 ǀ Page 43

Species Diversity and Molecular Phylogeny of Anguilliformes in Vietnam

Binh Dang Thuy, Oanh Truong Thi, Phuong Dinh Thi, Quyen Vu Dang Ha

Institute for Biotechnology and Environment, Nha Trang University

Corresponding author: Binh Dang Thuy ([email protected])

The order Anguilliformes is one of wild distributed marine and freshwater eel, play a

key role of marine ecosystems, however, their taxonomic relationships remain poorly

understood. The current study applied DNA barcoding for identifying 13 species of

marine eels (representing 4 families and 7 genera) collected from central (Khanh Hoa

and Phu Yen provinces), Vietnam. The phylogenetic relationships of the Anguilliformes

in Vietnam are examined using mitochondrial sequence data through Neighbour

Joining, Maximum Likelihood and Bayesian Inference approaches. The phylogram

showed strongly support for a monophyletic family Muraenidae, Congridae and

Ophichthidae. Two clades were clearly detected: Muraenidae formed distinct clade

from remaining families Congridae, Synaphobranchidae and Ophichthidae. Among 4

families examined, Synaphobranchidae exhibited paraphyly as Muraenesox spp. were

clustered to species of ophichthid species, while, Dysomma anguillare and Ilyophis

brunneus were sister species to Bathyuroconger spp. (Congridae). This is the first DNA

barcoding data of marine eel species in Vietnam, contributing significantly to the

assessment of crab biodiversity, and as a basis for resource management and

conservation. Additional sampling for Anguilla fish species was necessary to better

understand of their taxonomy and phylogeny.


O-S1 ǀ Page 44

Morphometric and Genetic Variations of Genus Barbonymus

(Pisces, Cyprinidae) harvested from Aceh Waters, Indonesia

Agung Setia Batubara2, Zainal A. Muchlisin1,6, Deni Efizon3, Nur Fadli1, Zairin

Thomy4, Afrita Ida Utami5, Mohd Nor Siti-Azizah6

1 Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, Indonesia 2 Doctoral Program in Mathematics and Sciences Application (DMAS), Graduate Program,

Syiah Kuala University, Banda Aceh, Indonesia 3 Faculty of Fisheries and Marine Sciences, University of Riau, Pekanbaru, Indonesia

4 Faculty of Sciences, Syiah Kuala University, Banda Aceh, Indonesia 5 School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia

6 Center for Marine and Fisheries Studies, Syiah Kuala University, Banda Aceh, Indonesia

Corresponding author: Zainal A. Muchlisin ([email protected])

The objective of the present study was to analyze the morphometric and genetic

variations of the three presumed taxa within Barbonymus group locally name as Nelah,

Lampam-A and Lampam-B. The Nelah samples were collected from Nagan Raya

District, while the Lampam-A and Lampam-B were collected from Aceh Tamiang

District, Indonesia. A total 150 individual of fish samples (50 individual of every taxon)

were measured for traditional morphometric characters, while 5 random fin tissue

samples from each taxon were taken and used for genetic analysis using COI gene, and

one outgroup (Rasbora sumatrana) from the Genbank was used for genetic data

comparison. The results of univariate (ANOVA) and multivariate (Discriminant

function analysis, DFA) analysis showed that Lampam-A and Lampam-B have an

overlapping morphological characteristics, while Naleh was discriminated distinctly.

The genetic data showed that Lampam-A and Lampam-B were grouped in the same

clade indicate monophyletic, while Naleh grouped in the different clade. Therefore,

there is an agreement data between biometric and genetic (COI) of Barbonymus. The

NCBI blasting showed that Lampam-A and Lampam-B are considered the same species,

Barbonymus schwanenfeldii; while Naleh is considered as B. gonionotus. The

intraspecies divergence between B. schwanenfeldii and B. gononiotus was 3.1%, where

the interspecific genetic divergence among B. schwanenfeldii was 0.5% and 0.0%

among B. gonionotus samples. It is concluded that the morphometric and genetic data

indicated two valid species of Barbonymus in Aceh waters i.e. B. schwanenfeldii and B.

gonionotus.


O-S1 ǀ Page 45

Phylogenetic Analysis (16sr RNA) of Rainbow trout of Azad Jammu and

Kashmir, Pakistan

Nuzhat Shafi1, Ulfat1, Ghazanfar Ali1, Javaid Ayub2, Tasleem Akhtar1

1 University of Azad Jammu and Kashmir, Muzaffarabad 2 Program Director Community development GoAJK

Corresponding author: Nuzhat Shafi ([email protected])

The intra specific divergence and phylogenetic analysis of rainbow trout (Oncorhynchus

mykiss) were examined; collected from four different hatcheries of Neelum valley viz

Pattika, Kuttan, Salkhalan and Dawarian of Azad Jammu and Kashmir from 2012 to

2014. Three different genes Cytochrome b, 16SrRNA and ATPase were used as

consistent genetic marker for phylogenetic analysis and intra specific divergence of the

species. Aforementioned study scrutinized the intra-specific sequence divergence of

Oncorhynchus mykiss on Cytb gene (1.4%) and ATPase gene (1.5%). While 16S rRNA

confirmed complete homology (99.3 -100 %) within samples of trout hatcheries as well

as with sequences of six other international Oncorhynchus species taken from NCBI.

Hence, it is considered to be conserve gene showing no divergence. The probability of

divergence may be due to water quality including temperature, pH, and DO etc.

Indicated study confirmed that Oncorhynchus species in attendance in Kashmir valley

belong to same phylogentic lineage with little nucleotide substitutions.


O-S1 ǀ Page 46

Phylogeography of Barbodes semifasciolatus Complex

(Teleostei, Cyprinidae) off Hainan Island and Adjacent Area

Shih-Pin Huang1, I-Shiung Chen2, 3, Thien Quang Huynh4, Yahui Zhao5, Chunguang

Zhang5, Nian-Hong Jang-Liaw6

1 Biodiversity Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan 2 Institute of Marine Biology, National Taiwan Ocean University, Jhongjheng, Keelung

20224, Taiwan 3 National Museum of Marine Science and Technology, Jhongjheng, Keelung 20248,

Taiwan 4 Southern Institute of Ecology, Vietnam Academy of Science and Technology 01, Mac

Dinh Chi Street, District 01, Ho Chi Minh City, Vietnam 5 Key Laboratory of Zoological Systematics and Evolution Institute of Zoology, Chinese

Academy of Sciences, Chaoyang District, Beijing 100101, China 6 Conservation Research Center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan,


Corresponding author: Nian-Hong Jang-Liaw ([email protected])

The spotted barbs Barbodes semifasciolatus complex is known for worldwide aquarium

trade. This species is native to southern mainland China, Hainan Island, Taiwan, and

northern Vietnam. This small cyprinid is notorious for its disputation of taxonomically

local varieties, morphs, and possible hybrids due to restocking. Consequently, so far the

phylogeographic analysis and their relationship between this species and other related

species is still remain unclear. Totally 130 individuals of P. semifasciolatus collected

from Hainan Island, southern mainland China, Taiwan and northern Vietnam which had

been sequenced with partial mitochondrial cytochrome b (927 bp in length) and COI

(633 bp) for attempting to infer their phylogeography. Additionally, two related

morphological species, B. snyderi and B. paucimaculatus also included for analyzing

their phylogeny. The existence of frequently gene flow have been observed in most

populations from Hainan Island. We speculated that it was caused by floodwaters,

forming routes and promote the gene flow of Hainan populations from each rivers in

the modern time. On the other hand, the population from Vietnam was found completely

nested within Hainan Island lineage. We speculated that it was caused by river

connection which connected Hainan and northern Indochina Peninsula during ice age

of the late Pleistocene. The preliminary recognized morphotypes of B. semifasciolatus

populations shows low correlation with defined clades of molecular analyses, regardless

of their apparently conspicuous morphological differentiation between populations.

According to present molecular and color patterns evidence, the species validity within

B. semifasciolatus complex and its related species will also be discussed.


O-S1 ǀ Page 47

Stream fishes (Teleostei) from Central Highlands and

South Central Coast of Vietnam

Hoang Duc Huy, Jean-Dominique Durand, Pham Manh Hung, Tran Trong Ngan

Lab of Zoology, Department of Ecology and Evolutionary Biology, University of Science –

Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Vietnam.

Corresponding author: Huy Duc Hoang ([email protected])

Stream fishes (Teleostei) of Southeast Asia drainages are largely species members of

orders Cypriniformes, Siluriformes, Synbranchiformes, and Perciformes living in clear,

running waters. Among them, cyprinids and catfishes are important groups of fish in

diversity and high economic values. In Central Highlands and South Central Coasts’

river basins of Vietnam, their taxonomy is still unsettled. Based on our last study in

Langbiang Plateau, the taxonomy of several groups (Onychostoma, Poropuntius, Tor)

is still not clear, with diversity patterns obscured by a perceived variability hiding

cryptic species. It therefore could be predicted that the higher species diversity and

complicated sympatric relationships to these species. The problem of these species

being misidentified due to uncertain diagnosis is the reason of lacking distributional

data or not represented in collections, or listed incorrectly on protected species lists

adding to the uncertainty. The purpose of this study is to examine taxonomy and

systematic of stream fishes and to resolve some of the outstanding issues regarding the

relationships within this group. To do this, both morphological and molecular biological

surveys were employed in this research. So, molecular phylogenetic studies are being

used to investigate fishes classification and evolution at species level in the same region

including their inter-relationships using nucleotide sequences from the mitochondrial

cytochrome (cyt) b and cytochrome c oxidase subunit 1 (CO1) genes and more recently

from nuclear DNA. The distribution of some morphological characters important in

classification; and biogeography of some taxa will be discussed. Furthermore, the study

will explore the systematic significance of the morphologies on the basis of the

comprehensive molecular phylogeny. Since then, this project will provide a natural link

in understanding concurrent geographical and biotic evolution of a given region. This

will be helpful information contributing for managing sustainable indigenous fish

resources in Central Highlands and South Central Coasts.


O-S1 ǀ Page 48

Biodiversity and Biogeography of Flatfishes (Teleostei: Pleuronectiformes)

living in Chinese Waters

Xiaoyu Kong1 and Hairong Luo1, 2

1 South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou,

China 510301 2 University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: Hairong Luo ([email protected])

As Pleuronectiformes in Fauna Sinica (Li and Wang 1995) recorded, flatfish consists

with three suborders, eight families, 50 genera and 134 species inhabiting off Chinese

waters. These tree suborders are Psettodoidei, Pleuronectoidei and Soleidei.

Psettodoidei only has one family. Pleuronectoidei includes five families, they are

Citharidae, Paralichthyidae, Bothidae, Pleuronectidae and Samaridae. And Soleidei has

two families, including Soleidae and Cynoglossidae. Comparing Li and Wang (1995)’s

work with the record of flatfish in Fishes of the world (Nelson 2016), we find eight of

14 families and 47 of 127 genera in the world (Nelson 2016) are consistent with that of

Li and Wang (1995)’s record. Therefore, family, genus and species diversities of

Pleuronectiformes living in Chinese Waters represent 57.1%, 39.4% and 17.4% of the

global diversities of flatfish, respectively. Around coastal waters of China, flatfishes are

widely distributed from cold temperate areas (Bohai and Yellow seas), warm temperate

waters (East China Sea), to subtropical and tropical regions (along southern coast from

Taiwan Straits westward to South China Sea). Because flatfish is the common costal

fish, identifying valid status of its species and distinguishing difference between each

species are not only the foundational works of relative fishery, but also the key to do its

biological and ecological researches. However, comparing morphological characters of

specimens from our fish collection of Chinese coastal waters with previous records of

corresponding species, we found several species are questionable with uncompleted

record of various pigmentations of same species. As well as, several potential new

species living in Chinese waters are not recorded. To facilitate the knowledge of species

diversity of flatfish, this study will focus on questionable species, do available fish

collections covering different seasons, and combine morphology and molecular

methods, to analysis taxonomic status of flatfish living in Chinese waters.


O-S1 ǀ Page 49

Insight into the phylogeny of Asian Spiny eel

(Synbranchiformes: Mastacembelidae): Implication for their Diversity,

Taxonomy and Biogeography

Jsari Amirul Firdaus Jamaluddin1, Nam So2,3, Bui Minh Tam4, Tran Thi Thuy Ha5,

Amirrudin Ahmad6, Mohd Shalahuddin Adnan7, Yuichi Kano8, Preeda Phumee9,

Chaiwut Grudpan10, Myint Soe11, Md. Latiful Islam12, Heok Hui Tan13, Mohammad

Sadegh Alavi-Yeganeh14, Arunachalam M.15, Lawrence M. Page16, Richard L.

Mayden17, Siti Azizah Mohd Nor1 1 School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia

2 Inland Fisheries Research and Development Institute (IFReDI), Fisheries Administration, Phnom Penh,

Cambodia 3 Mekong River Commision, Sangkat Chak Angre Krom, Khan Menachey, Phnom Penh, Cambodia

4 Department of Freshwater Aquaculture, College of Aquaculture and Fisheries, Can Tho University,

Can Tho city, Vietnam 5 Centre of Aquaculture Biotechnology, Research Institute for Aquaculture No1, Dinh Bang - Tu Son -

Bac Ninh, Viet Nam 6 School of Marine and Enviromental Sciences, Universiti Malaysia Terengganu, Kuala Nerus,

Terengganu, Malaysia 7 Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia, Johor,

Malaysia 8 Institute of Decision Science for a Sustainable Society, Kyushu University, Fukuoka, Japan

9 Faculty of Science and Fisheries and Technology, Rajamangala University of Technology Srivijaya

Trang Campus, Trang, Thailand 10 Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani,

Thailand 11 Insitute of Fisheries Technology, Department of Fisheries, Yangon, Myanmar

12 Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna, Bangladesh 13 Lee Kong Chian Natural History Museum, National University of Singapore, Singapore

14 Department of Marine Biology, Faculty of Marine Sciences, Tarbiat Modares University Noor,

Mazandaran, Iran 15 Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University,

Tamilnadu, India 16 Florida Museum of Natural History, University of Florida, Florida, USA

17 Department of Biology, Saint Louis University, St. Louis, Missouri, USA

Corresponding author: Jsari Amirul Firdaus Jamaluddin ([email protected])

The family Mastacembelidae is the most species-rich of the order Synbranchiformes

and widespread throughout tropical and subtropical regions of Africa and Asia.

However, phylogenetic relationships amongst Asian mastacembelids have never been

tested. The current study utilised a combination of mitochondrial (cytochrome b and

cytochrome oxidase c subunit 1) and nuclear DNA (first intron of the nuclear S7

ribosomal protein) to investigate its evolutionary relationships. The major lineages

concur with the currently proposed morphological species groups except for the

Mastacembelus armatus species group. The findings also revealed greater diversity than

previous studies based on morphological approach and uncovered deep intraspecific

lineage diversity which is attributed to low dispersal capabilities, ancient evolutionary

history, biogeographical patterns, dynamic river systems, or even cryptic speciation.

Referring to Mastacembelidae as an example, even in well-studied river systems,

freshwater fish diversity in this region is still underestimated and far from resolved.


O-S1 ǀ Page 50

Tropical marine fish aggregations along the eastern coast of Kyushu, Japan,

northwestern Pacific Ocean, with an assessment of influence of

the Kuroshio Current

Ryohei Miki1, Atsunobu Murase1, Masaaki Wada2, Keita Koeda3, Kazuo Hoshino4,

Hiroyuki Motomura5, Hiroshi Senou6

1 Nobeoka Marine Science Station, University of Miyazaki 2 Fisheries Cooperative Association of Iorigawa

3 National Museum of Marine Biology & Aquarium 4 Oita Marine Palace Aquarium, UMITAMAGO

5 Kagoshima University Museum 6 Kanagawa Prefectural Museum of Natural History

Corresponding author: Ryohei Miki ([email protected])

Four major distributional patterns of tropical fishes occurring in inshore waters of

southern Japan have been recognized: pattern 1: distributed only in tropical/subtropical

islands (such as the Ryukyu islands); pattern 2: continuously distributed from

tropical/subtropical islands to a temperate region; pattern 3: mainly distributed in

tropical/subtropical islands, and discontinuously occurring in a temperate region; and

pattern 4: occurring only in a temperate region. The latter two discontinuous

distributional patterns are most likely to be formed by an influence of transportation of

the Kuroshio Current which flows from the northeastern part of the Philippines to the

Pacific coasts of Japan, via the western side of the Ryukyu Islands.

Kyushu, the southernmost main island of Japan, faces the Pacific Ocean eastward and

the East China Sea westward. Marine fish species diversity and distribution along the

eastern coast of Kyushu (composed of Oita, Miyazaki, and part of Kagoshima

prefectures) are poorly known, although a study on fishes in the area is important to

understand fish diversity in Japanese waters as the area lengthening north to south is

located at the upper reaches of the Kuroshio Current in a domestic temperate region. To

assess an influence of the Kuroshio Current against coastal fish fauna of a temperate

region in Japan in terms of space and time, occurrence of “sporadic tropical species

(STS)” (defined here as tropical coastal fishes showing discontinuous distribution

patterns in a temperate region of Japan: above-mentioned patterns 3 and 4) in eastern

Kyushu was investigated, focusing latitudinal gradients of fish diversity and seasonal

changes of the number of species. In this presentation, we give preliminary results of

relationship of fish diversity in eastern Kyushu and the Kuroshio Current.


O-S1 ǀ Page 51

Variations of otolith morphology of larval and juvenile yellow sea bream

Acanthopagrus latus (HOUTTUYN, 1782) collected from northern Vietnam

Hoang Kim Duyen

Hanoi National University of Education

Corresponding author: Hoang Kim Duyen ([email protected])

Otolith are the structures located in the inner ear cavity of all teleost fish. Each side

includes sagittae, lapilli and asterisci that are different in shape, size and location. The

morphology of otoliths has been used in species identification, age and growth

determination, larval ecology, fish stock identification and environmental

reconstruction of the fish habitats. However, little is known about otolith morphology

during early stages of fish. To examine whether otolith could be used as a precise tool

in identification of larvae and juveniles, this study spent a total of 157 specimens (10.2-

14.7 mm standard length) of yellow sea bream larvae and juveniles collected by a seine

net at the bank waters of the Kalong and Tien Yen Rivers, northern Vietnam. A total of

254 sagittae, 109 lapilli, and 27 asterisci were removed and analyzed. The data showed

that otolith morphology was changed with growth in Acanthopagrus latus larvae and

juveniles. The sagittae and asterisci were the largest and the smallest otolith,

respectively. Sagittae were oval-shaped and changed remarkably in shape with growth.

The shape of sagittae show an asymmetry between the right and the left sides in this

species. The shape and size of sagittae were also different among the two rivers, and it

can be an evident to conclude that otoliths can not use as a precise tool in identification

during this fish’s early stages.


O-S1 ǀ Page 52

Structure and function of corneal surface of mudskipper fishes

Wenxian Hu1, 2, Bin Kang2, Jie Zhang1

1 Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology,

Chinese Academy of Sciences. 2 School of Life Sciences, Yunnan University

Corresponding author: Jie Zhang ([email protected])

Natural selection plays a key role in the adaptive evolution and attracts wide attention

on theoretical research in biology. To adapt their different inhabiting environments, fish

species have formed corresponding unique sensing, motion and respiration system

through the long process of evolution. These morphological or physiological

specializations are obviously outstanding especially for fishes which live in special

habitats. Oxudercine Gobies, which live at intertidal area, are described as a unique

transitional biota showing specialized tissues and organs to adapt to their amphibious

life. Vertebrate corneal epithelium cell plays an important role for imaging, and the cell

density, together with the appearance or type of affiliated microstructures, is considered

as a result of evolution adapting to alternate terrestrial or aquatic environment. Here we

investigated the corneal cells of both larvae and adult amphibious mudskippers

Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus, to testify the

relationship between morphology and function. The cell density values of the two

species were 31,137 and 31,974 cells per mm2 in larvae and then significantly decreased

to 15,826 and 25,954 cells per mm2 in adult (P<0.001), respectively, which could be

explained as the habitat change from aquatic to different degrees of terrestrial

environment. The corneal epithelium cells were ridge type in larvae and differentiated

into ridge type and reticular type in adult P. magnuspinnatus and ridge type, reticular

type and ridge–reticular type in adult B. pectinirostris. Four kinds of microstructures as

microridge, microvilli, microplicae and microhole appeared in both species. In addition,

corneal and habitat data correlation analysis showed a strong correlation between the

microridge width and habitat, with a correlation coefficient of 0.8, and corneal cell

microridge width appears gradually narrowing trend from aquatic transition to the

terrestrial environment. Such difference of microridge width and its separation indicated

that a dense cell connection was requested in a saltier and more terrestrial environment.


O-S1 ǀ Page 53

Sweepers (Teleostei: Perciformes: Pempheridae) in the Ogasawara Islands,

Japan, with an undescribed species of the genus Pempheris

Keita Koeda1, Hiroyuki Motomura2

1 National Museum of Marine Biology & Aquarium. 2 Houwan Road, Checheng, Pingtung,

94450, Taiwan

2 The Kagoshima University Museum

Corresponding author: Keita Koeda ([email protected])

Six species of family Pempheridae, Parapriacanthus ransonneti, Pempheris adusta, Pe.

oualensis, Pe. schwenkii, Pe. ufuagari, and Pe. sp. were confirmed from the Ogasawara

Islands on the basis of a field survey and the vouchered specimens in the museum

collection. Pempheris sp. is most similar to Pe. japonica, endemic to Japanese and

Korean waters, in having adherent scales with strong ctenii on the lateral and ventral

surfaces of the body. However, the former can be distinguished from the latter by the

several scale counts, and the presence of a distinct blackish blotch on the pectoral-fin

base. Pempheris sp. and Pe. ufuagari are restricted to the Ogasawara Islands and the

oceanic islands of Japan, including the Ogasawara Islands, respectively.

Parapriacanthus ransonneti and Pe. schwenkii are temperate water species basically

distributed around the Japanese mainland. In contrast, Pe. adusta and Pe. oualensis are

tropical water species that does not or rarely occur around the Japanese mainland. The

distributional pattern of each species of the family Pempheridae occurring in the

Ogasawara Islands demonstrates its microcosm of the unique biodiversity in the islands

where temperate, tropical, and oceanic islands species co-occur in such small islands.


O-S1 ǀ Page 54

An undescribed species of the anchovy genus Stolephorus

(Clupeiformes: Engraulidae) from Southeast Asia

Harutaka Hata1, Hiroyuki Motomura2

1 United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24

Korimoto, Kagoshima 890-0065, Japan 2 The Kagoshima University Museum

Corresponding author: Harutaka Hata ([email protected])

During a revisionary study of the genus Stolephorus (Clupeiformes: Engraulidae), 32

un-identified specimens of the genus were collected from Southeast Asia. The

specimens are similar to S. chinensis (Günther, 1880) in sharing almost the same

numbers of gill rakers and scutes, the maxilla reaching posteriorly to the preopercular

margin, a pair of dark patches behind the occiput without following a pair of dark lines,

the rounded preopercular margin, and no black spots below eye or tip of lower jaw. The

un-identified specimens however differs from S. chinensis in having a lower number of

the transverse scales (8 vs. 10 in the latter), a deeper body (19.7–24.1% SL vs. 16.0–

18.9%), a longer dorsal-fin base (15.0–17.6% SL vs. 13.7–15.8%), and a wider

interorbital area (25.0–26.6% HL vs. 21.7–24.2%). Although the un-identified

specimens were collected only from Malaysia and Indonesia, S. chinensis was known

only from Amoy, China to Ha Long Bay, northern Vietnam. It suggests that the two

species are distributed allopatrically. In the clupeiform fishes, some paired sister species

are also known to occur allopatrically in northern and southern Vietnam, indicating that

the boundary line for the two paired sister species’ distributions might be located at the

middle part of Vietnam.


O-S1 ǀ Page 55

Truss morphometric analysis of mullets (Mugillidae) harvested from

Lambada waters, Aceh Besar district, Indonesia

Derita Yulianto1, Zainal Abidin Muchlisin2, Agung Setia Batubara1, Indra Zainun3

1 Doctoral Program in Mathematics and Sciences Application (DMAS), Graduate Program,

Syiah Kuala University, Banda Aceh, Indonesia 2 Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh 23111, Indonesia

3 Faculty of Agriculture, Syiah Kuala University, Banda Aceh, Indonesia

Corresponding author: Zainal Abidin Muchlisin ([email protected])

The objective of the present study was to examine the morphometric variations of the

mullets (Mungillidae) in Lambada waters, Aceh Besar District, Indonesia. The sampling

was conducted from January to March 2017 by using gillnets with the mesh size of 1.0

to 2.0 inches. Four species of mullets were recorded during the study i.e. Crenimugil

heterocheilos, Liza melinoptera, L. subviridis, and L. tade. A total of 60 samples of each

species (240 samples totally) were measured for truss morphometric involved 24

characters. The data were subjective to Discriminate Function Analysis (DFA). The

DFA analysis resulted in three functions i.e. Function 1 has the eigenvalue of 57.11

explains 99.1% of total variants, while function 2 and function 3 have eigenvalues lower

than 1 and both functions explain 0.9% of total variants. The scatter plot of DFA showed

that there were two groups of mullets where C. heterocheilos, Liza melinoptera, L.

subviridis were grouped into the same group, while L. tade was discriminated distantly.

The fish species within the first group had higher morphology similarity and these

species were different significantly with the second group of L. tade.


O-S2 ǀ Page 56

Study of Ichthyofauna of River Poonch, Azad Jammu and Kashmir Pakistan

Javaid Ayub1, Nuzhat Shafi2, Tasleem Akhtar2, Danish Kiani2, Atiya Zareen2

1 Director Fisheries Government of Azad Jammu and Kashmir, Block no. 5, Room no. 4

New civil sectt.Chattar Muzaffarabad AJK 2 University of Azad Jammu and Kashmir, Muzaffarabad

Corresponding author: Javaid Ayub ([email protected])

A survey was conducted to analyze the overall status of ichthyofauna from different

localities of River Poonch in 2012. River Poonch is the habitat of many endangered and

threatened species of the world. Collected fishes were identified. which belong to 3

Orders, 5 Families, 13 Genera and 16 Species. According to this study, Cyprinidae was

the richest Family, which constituted ten Species. Whilst Sisoridae were characterized

by two species. Nemacheilidae, Cobitidae and Mastacembelidae were illustrated as

single species each. Statistically, fish fauna richness of River Poonch was significantly

high. Nonetheless, the aquatic environment of the river was badly affected by

anthropogenic activities like extraction of gravel, sand stones, use of illegal methods for

fishing like dynamiting, electro-fishing, extensive use of fixed nets, poisoning and

pollution due to untreated household sewage running directly in to river. Keeping in

view the importance of river Poonch, its fauna and last resort for endangered fishes like

Mahseer, the government of AJK on advice of department of wildlife and fisheries and

Himalayan Wildlife Foundation (HWF), declared River Poonch as “Mahseer National

Park” the country’s first ever aquatic national park.


O-S2 ǀ Page 57

Dynamic conservation plan on species invasion revealed by spatial and

temporal changes in invasion risk

Chunlong Liu1,2, Lise Comte2, Julian D. Olden2, Weiwei Xian1

1 Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology,

Chinese Academy of Sciences, Qingdao 266071, China 2 School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, USA

Corresponding author: Weiwei Xian ([email protected])

Determining holistic national action to mitigate impacts of species invasion plays a key

role on biodiversity conservation. However, current national plans on preventing

invasionsare almost static without considering establishment of potential species and

expansion of established species. To provide suggestions on setting dynamic plan for

conserving China’s biodiversity, we identified establishment risk of nonnative

freshwater fish across China under three scenarios (i.e. current, 2050s, 2070s) and

explored future changes in habitat suitability. All nonnative species were first grouped

into aquaculture and ornamental species based on introduction vector, and then

classified into established and potential species based on establishment status. For each

group, we quantified their establishment risk by overlapping introduction risk with

habitat suitability across China. Changes in habitat suitability were calculated to predict

their extraction and expansion in the future. Species invasiveness under each scenario

was defined as projected suitable area (sum of cell probability * cell area). Our results

indicated southeastern China has high establishment risk and western Tibet has high

suitability but low introduction risk for all groups. Different groups also exhibited

obvious differences in establishment risk in different regions. In the future, habitat

suitability would increase in northern and central China and decrease in southern China,

with the increase in 2050 being much higher than 2070. Increase of species invasiveness

is also higher in 2050 than 2070. Our research therefore revealed the importance for

setting dynamic conservation plans on preventing species invasions according to

regional differences in establishment risk and future changes in habitat suitability.

Conservation resource should therefore be reasonably allocated in introduction and

establishment stages; and eradication strategies should be adjusted according to future

climates.


O-S2 ǀ Page 58

Invasiveness screening of non-native freshwater fishes in the middle reach of

the Yarlung Zangbo River, Tibetan Plateau, China

Shan Li, Jiakuan Chen, Xiaoming Wang, Gordon H. Copp

Natural History Research Center, Shanghai Natural History Museum, Branch of Shanghai Science & Technology Museum, Shanghai, 200041

Corresponding author: Shan Li ([email protected])

The aim of the present study was to identify potentially invasive non-native freshwater

fishes in the middle reach of the Yarlung Zangbo River, on the Tibetan Plateau, China,

using the Aquatic Species Invasiveness Screening Kit (AS-ISK), as decision-support

tool. Based on the independent evaluations of 24 non-native freshwater fishes, a

threshold score of 29 distinguished species likely to pose a high risk of becoming

invasive (≥29) from species likely to pose low-to-medium risk (<29) in the risk

assessment (RA) area. Nine species were categorized as “high risk”: goldfish Carassius

auratus, topmouth gudgeon Pseudorasbora parva, brook trout Salvelinus fontinalis,

Oriental weatherfish (a.k.a dojo gudgeon) Misgurnus anguillicaudatus, Siberian taimen

Hucho taimen, and common carp Cyprinus carpio, peled Coregonus peled, western

mosquitofish Gambusia affinis, and Chinese rice fish Oryzias sinensis. The three lowest

scoring species were Arctic cisco Coregonus autumnalis, Wuchang bream

Megalobrama amblycephala, and Chinese ice fish Neosalanx taihuensis, which are

unlikely to be invasive because they are unable to complete their life cycle in the RA

area. Climate change assessments scores increased or remained the same for warm-

water species, and decreased for coldwater species. Confidence factors for the

assessments were from 0.65–0.91 with the mean of 0.81 ± 0.02 SE. This study was the

first application of AS-ISK in western China, and the results suggest that AS-ISK is a

useful and valid tool for identifying potentially invasive risk aquatic species in China.


O-S2 ǀ Page 59

Survival Status of Rare and Endemic Schizothorax

(Cypriniformes: Cyprinidae) in Lugu Lake, Northwestern Yunnan of China

Xu Li1, Wei Zhou1*, Lian-xiang Li2

1 Faculty of Forestry, Southwest Forestry University, 300 Bailongsi Road, Kunming

650224, Yunnan, China, +86 (0)871 63862458 2 Lugu Lake Provincial Nature Reserve Management Bureau, Lijiang, 674300, Yunnan,

China, +86 (0)888 5881027

Corresponding author email address: Xu Li ([email protected]), Wei Zhou ([email protected])

Schizothorax labrosus, S. microstomus and S. ninglangensis are three specialized

sympatric endemic fish species in Lugu Lake, northwestern Yunnan of China. Their

differentiation history was also a typical case of fish specialization in plateau lakes in

Yunnan with a great scientific value for analyzing the historical formation of the

Qinghai-Xizang Plateau and for studying on the influence of species differentiation in

sympatric organisms. Continually threatened by many factors such as introduction of

exotic fish, over-fishing and water quality decline, the population of three species of

Schizothorax has been decreased year by year. At present, these species are extremely

rare and even once considered to be extinct. We set up 2 transect lines in the spawning

and foraging sites of the fish in Lugu Lake, and the composition of fish in Lugu Lake

was studied by means of underwater sonar image records and specimen collection from

January to June in 2017. Eight sections of underwater sonar images were recorded and

60 times were fished by nets. After measured the body length and width of the captured

fish specimens, morphological models for different fish species were built. The fish

individuals from sonar images would be identified and coded firstly. Matching the

image individual characteristics of body length and width with the characteristics of

morphological model, the species would be determined. The results showed that there

are 15 species in Lugu Lake, among which 12 are exotic species, including

Paramisgurnus dabryanus, Clenopharyngodon idellus, Cyprinus carpio, Carassius

auratus, Rhodeus sinensis, Abbottina rivularis, Pseudorasbora parva, Silurus

meridionalis, Protosalanx hyalocranius, Micropercops swinhonis, Rhinogobius

giurinus and Gambusia affinis. There are two native species, Misgurnus

anguillicaudatus and Schizothorax microstomus. Besides Schizothorax microstomus,

other Schizothorax individuals were found in sonar images that were speculated to be

Schizothorax ninglangensis according to account of historical documents. However,

Schizothorax labrosus was not found in this investigation. The 2 species individual

numbers of Schizothorax found in this investigation are less than 1‰ of the total

individuals from Lugu Lake, and they are already on the verge of extinction. The

increase of fishing intensity is one of the important reasons for the decline of the yield

of early Schizothorax. Another reason is the introduction of exotic fish species into Lugu

Lake. Because the food habits of many fish species are similar to or overlap with the

food habits of the three species of Schizothorax, coupled with competition for living

space, native fish are often at a disadvantage. Therefore, the fish composition in the lake

has been dominated by alien species.


O-S2 ǀ Page 60

Diversity and species composition of fish community of

Peninsular Malaysia small streams

Amirrudin B. Ahmad

Universiti Malaysia Terengganu, School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

Corresponding author: Amirrudin B. Ahmad ([email protected])

Fish communities of Oriental region, particularly the small streams are diverse, but

basic pattern of species richness, distribution, abundance and spatial similarities are still

poorly described. To document these patterns and to quantify the relative magnitudes

of spatial variation in species composition, small stream fish communities in Peninsular

Malaysia were studied. Fish were collected from 50 small streams across the country.

A total of 103 fish species were identified from 15181 specimens collected from 50

small streams throughout Peninsular Malaysia, with members of the family Cyprinidae

predominant. The majority of those recorded were native to Peninsular

Malaysia but two (Esomus metallicus and Trichopodus pectoralis) were introduced

species. The main findings is that many fishes exhibited discontinuous patterns of

distribution and were considered to be rare while only a handful were widely distributed

and abundant. Ordination based on the relative resemblance of fish communities to one

another support the existence of two distinct ichthyogeographic divisions in Peninsular

Malaysia. In addition, a degree of dissimilarity between regions was observed in terms

of species richness and ‘diagnostic’ species, despite frequent species overlap between

some regions. It is recommended that a sizeable augmentation of the existing protected

areas is needed to safeguard Malaysia’s exceptionally diverse stream-dwelling fauna of

which fishes are simply the most well-known inhabitants.


O-S2 ǀ Page 61

Fish diversity and emergence of non-native species in rice agroecosystem in

northern Peninsular Malaysia

M. Aqmal-Naser, Amirrudin B. A.

University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Kenyir

Research Institute, University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

Corresponding author: Amirrudin B. A. ([email protected])

Twenty six species from 14 families were recorded with Cyprinidae being the dominant

family (9 species) followed by Osphronemidae (3 species), Clariidae (2 species) and

Bagridae (2 species). Most of the individuals were collected from family Cyprinidae

(3778 specimens), Anabantidae (1239) and Aplocheilidae (544 specimens) from the

river, concrete canals, earth ditches and storm drains at the rice field, during the three

different stages of paddy planting for two seasons of rice cultivation. There were seven

non-native species recorded in this study namely, Barbonymus gonionotus, Clarias

gariepinus, Esomus metallicus, Gambusia affinis, Hypostomus plecostomus,

Trichopodus pectoralis, and Oreochromis niloticus. African catfish and Nile tilapia

were considered as invasive species due to their capabilities to overpopulate the

ecosystem and competing with the native species for food and breeding ground. Other

introduced species such as Barbonymus gonionotus and Trichopodus pectoralis were

however produce less impact to native species and are the favorable fishes among locals.

The emergence numbers of invasive species can threatening the native species

population in the rice field, and this need further studies on how they can replace the

indigenous species and lead to the local extinction.


O-S2 ǀ Page 62

Functional diversity and vulnerability of freshwater fishes in Sundaland:

making fair comparisons across unevenly sampled ecoregions

Kenny WJ Chua, Darren CJ Yeo

National University of Singapore/Department of Biological Sciences, 14 Science Drive 4,

Singapore 117543, Republic of Singapore

Corresponding author: Kenny WJ Chua ([email protected])

In Sundaic Southeast Asia, optimal functioning of freshwater ecosystems is threatened

by potential extirpations of fish species due to rapid and widespread forest habitat loss.

The functional diversity of Sundaic ichthyofauna represents a crucial link between

biodiversity and ecosystem functioning, and assessing this functional diversity is an

important first step in understanding potential impacts on ecosystem functioning.

However, fair comparisons of functional diversity across Sundaic freshwater ecoregions

are hampered by differences in taxonomic coverage between ecoregions. In this study

we aim to understand the variation in functional diversity of fishes across freshwater

ecoregions of Sundaland, and its vulnerability to potential species losses associated with

forest habitat conversion. To do so, we apply a rarefaction and extrapolation framework

to fairly compare functional diversity of Sundaic ecoregions at a standardised level of

sampling coverage. We also make use of Hill numbers to assess functional diversity in

terms of both functional richness and functional evenness. Lastly, we apply a matrix-

calibrated species-area model to predict how functional diversity might be impacted by

continued forest and species loss. The findings provide the first ecoregion-scale

assessment of functional diversity of freshwater fishes across Sundaland, revealing

insights on the stability of ecosystem functioning against forest loss, thereby informing

conservation prioritisation efforts.


O-S2 ǀ Page 63

Larval and juvenile ichthyofauna in estuaries of the northern Vietnam

Tran Duc Hau1, Ta Thi Thuy2, Izumi Kinoshita3, Tran Trung Thanh3

1Hanoi National University of Education, Vietnam

3Kochi University, Japan 2Hanoi Metropolitan University, Vietnam

Corresponding author: Tran Duc Hau ([email protected])

Estuarine fish assemblages are often dominated by larvae and juveniles from the

spawners inhabiting sea, so that estuarine biotopes function as nursery grounds during

transforming from larval to juvenile stages for the coastal fishes. Although the northern

Vietnam is located in tropical zone latitudinally, the coastal ichthyofauna resembles

rather a temperate’s one, and can display transitional biodiversity from tropical to

temperate waters. However, little has been studied about estuarine assemblage of fish

early stages in Vietnam, so that we investigated the larval and juvenile ichthyofauna in

estuaries on the northern coast of Vietnam for a detailed understanding of the fish

community structure. Collections of larvae and juveniles were made along the bank

waters in three estuaries (of the Kalong: ca. 21°30’N, Tien Yen: ca. 21°20’N and So

Rivers: ca. 20°10’N) of the northern Vietnam from 2013 to 2015. During this period, a

total of ca. 100,000 fishes of more than 74, 46 and 45 species from 29, 25 and 23

families, were collected in the Kalong, Tien Yen and So estuaries, respectively. Three

estuary communities were occupied by larvae and juveniles belonging to chiefly

Gobiidae, Sparidae, Sillaginidae, Leiognathidae and Ambassidae, and were little

different. Most of fishes from these families were distributed as late larvae to early

juveniles in developmental stage. They occurred more abundantly in wet (May-October)

than dry (November-April) season in any estuaries.


O-S2 ǀ Page 64

Spatial Variation of Gobiid Species Assemblage in Mudflat and

Mangrove Habitat Along Coastlines in the Mekong Delta

Dinh Minh Quang1, Tran Dac Dinh1, Tran Quoc Ngu1.2, Tran Thi Hoa1,3,

Truong Thi Truc1,4, Tran Thuy Trang 1,5 and Le Thi Truc Linh1,6

1 Can Tho University, Can Tho 900000, Vietnam

2 Luong Tam High School, Hau Giang 950000, Vietnam

3 Nguyen Thi Minh Khai Specializing High School, Soc Trang 940000, Vietnam

4 Tap Son High School, Soc Trang 940000, Vietnam

5 Nguyen Dinh Chieu Specializing High School, Dong Thap 870000, Vietnam

6 Phan Van Hung High School, Soc Trang 950000, Vietnam

Corresponding author: Dinh Minh Quang ([email protected])

This study provides data on species composition and diversity of families Gobiidae and

Eleotridae in the mudflat and mangrove habitat along the coastline in the Mekong Delta.

A total of 37,133 individuals was sampled using bottom gill nets, fishing rods and hands

at five sites from June 2016 to May 2017. The PRIMER 6.0 was performed to calculate

the biodiversity indices of gobiid assemblage. The results showed that 45 species

belonging to 26 genera and families Eleotriade and Gobiidae were recorded in the study

sites. Of 45 species, family Eleotridae comprised eight species accounting for 17.78%

whereas 37 species (78.22%) were recorded for family Gobiidae. The gobiid

assemblage displayed a high Shannon-Weaver (H’=1.21±0.01 SE), species richness

(d=4.22±0.07 SE) species evenness (Pielou=0.84±0.01) and inverse dominance (1-

=0.91±0.01 SE) indices. These four indexes varied with study sites not seasons and

months. The gobiid richness and inverse dominance indices reached the highest point

in Ganh Hao (4.61±0.15 SE and 0.92±0.04 SE) but lowest in Tran De (3.74±0.12 SE

for d) and Nha Mat (0.90±0.01 SE for 1-). The Pielou index displayed the greatest in

Tran De (0.86±0.01 SE) but lowest in Nha Mat (0.82±0.11 SE), the Shannon-Weaver

index was highest in Ganh Hao (1.27±0.03 SE) but lowest in Duyen Hai (1.19±0.02

SE). The fish composition in the study areas was quite abundant but some commercial

fish rarely were found, indicating that local government should establish an effective

plan for future management and exploitation fish resources.


O-S2 ǀ Page 65

Composition and Diversity of Phytoplankton in Relation to Environmental

Variables and Seasonality in a Tropical Mangrove Estuary, Malaysia

A.S.M. Saifullah1, 2, Abu Hena Mustafa Kamal1, Mohd Hanafi Idris3, Amy Halimah

Rajaee1

1 Department of Animal Science and Fishery, Universiti Putra Malaysia Bintulu Sarawak

Campus, 97008 Bintulu, Sarawak, Malaysia 2 Department of Environmental Science and Resource Management, Mawlana Bhashani

Science and Technology University, Santosh-1902, Tangail, Bangladesh. 3 School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Kuala

Terengganu, Terengganu Darul Iman, Malaysia

Corresponding author: Abu Hena Mustafa Kamal ([email protected])

Phytoplankton community and abundance of a tropical mangrove estuary were

investigated in Sarawak, Malaysia. Data were collected monthly from January 2013 to

December 2013, and was pooled into seasons to examine the influence of seasonality.

The estuary revealed relatively species-rich and a total of 102 species under 43 genera

were recorded comprising 6 species of Cyanophyceae; 4 species of Chlorophyceae; 63

species of Bacillariophyceae and 29 species of Dinophyceae. The species composition

as well as the mean abundance (cells/L) of phytoplankton were found in an order of

Bacillariophyceae>Dinophyceae> Cyanophyceae> Chlorophyceae. Mean abundance of

phytoplankton ranged from 5694 to 88890 cells/L over the study period with its higher

value in the dry season. Species recorded from the estuary, were dominated by

Pleurosigma normanii, Coscinodiscus sp., Coscinodiscus centralis, Coscinodiscus

granii, Dinophysis caudata, Ceratium carriense, Ceratium fusus and Ceratium

lineatum. Abundance of phytoplankton demonstrated positive correlation with

chlorophyll a (r=0.69), ammonium (r=0.64) and silica (r=0.64). Significant differences

(ANOSIM and nMDS) were observed in the species community structure between

intermediate and the wet season. The species assemblages were positively correlated

with surface water temperature, salinity, pH, ammonium and nitrate in the intermediate

and dry season towards larger species composition in the respective seasons whereas

silica showed influence on the species assemblage in the wet season. The findings

suggest that the Sibuti mangrove estuary is productive in terms of diversified fishery

resources, which are influenced by the hydrobiological factors.


O-S2 ǀ Page 66

Reproductive Biology and Histological Study of Gonads of Keeled Mullet

(Chelon carinata Valenciennes, 1836) in the Persian Gulf and Oman Sea

Javad Ghasemzadeh, Abdolhamid Aran

Chabahar Maritime University - Faculty of Marine Sciences - Iran, Chabahar Maritime

University - Faculty of Marine Sciences- Daneshgah Blvd. Chabahar - Iran. Post code:

99717-56499

Corresponding author: Javad Ghasemzadeh ([email protected])

This study aimed to study the reproductive biology and histology of gonad development

of keeled mullet (Chelon carinata Valenciennes, 1836) in the waters of the Persian Gulf

and Oman Sea along the south-eastern coast of Iran. For this purpose mullet specimens

were collected monthly from coastal waters of the Jask, Tang, Konarak, Chabahar Bay,

Pasabandar and Gwater along the Oman Sea and Bandar Abbas, Gheshm Island,

Bushehr, Genaveh, Abadan and Khorramshar fishing areas in Persian Gulf during May

2016 to April 2017. Among the overall samples, a total of 450 keeled mullets were

collected. After measurement and recording the biometry and total weight of each fish,

the gut content was investigated and the gonads were taken, weighed and stored in %10

formaline for 48 hours, then thoroughly washed with pure water and transferred into

70% ethanol. The gonad samples were prepared for histological observation by standard

method of hydration, clearing and imbedding in paraffin. Then 5 micron sections of the

tissues were prepared by microtome and stained by hematoxillin-eozine method, and

studied under a light microscope. The gondo-somatic index, macro and microscopic

changes of gonads in different months were investigated. The results revealed that

Keeled mullet displayed an isochronal spawning pattern, and the ovarian maturation

cycle of this species had six stages of non-mature, resting stage, developing stage,

advanced or ripe stage, spawning stage and spent or spawned stage. The spawning of C.

carinatus in the Oman Sea occurred during March to early April, while in the Persian

Gulf it happened from late March towards the end of April and early May.


O-S2 ǀ Page 67

Application of isotopic analysis to reconstruct habitat use of Japanese sea

bass (Lateolabrax japonicas) and discrimination from reared fish

Jen-Chieh Shiao, Yu Shieh, Saulwood Lin

National Taiwan University / Institute of Oceanorgraphy, No. 1, Sec. 4, Roosevelt Rd,

Taipei, Taiwan 10617

Corresponding author: Jen-Chieh Shiao ([email protected])

Japanese sea bass (Lateolabrax japonicas) widely distributed from Japan to the South

China Sea may show diadromous migration. However, the migratory behaviors of this

species are rarely studied and still unclear. Japanese sea bass is a food fish providing

from fish farming or fishery catch of wild individuals. Stable isotopic analysis of

otoliths and muscles were conducted to clarify the habitat use of wild Japanese sea bass

and to discriminate the wild population from the cultured fish. Otolith δ18O values and

water temperature of the corresponding period were used to predict the salinity

experienced by the fish. The estimated salinity profiles suggested that some Japanese

sea bass entered the estuaries and rivers to forage at different life stage. However, some

fish resided in the marine environment during its whole life. The stable isotopic

composition were more variable in the wild fish (δ18O: -3.02 ± 1.3‰; δ13C: -4.92 ±

1.8‰, n=18) than in the reared fish (δ18O: -4.11 ± 0.3‰; δ13C: -9.08 ± 0.6‰, n=7). In

addition, the stable isotope composition of otolith (K-W test, δ18Ooto: p = 0.025;

δ13Coto: p = 0.001) and muscle (K-W test, δ14Nmus: p = 0.036; δ13Cmus: p <0.001)

were significantly different between the hatchery and wild fish. These results suggested

that Japanese sea bass can use diverse habitats from rivers, estuaries to oceans. The

stable isotopic compositions of otoliths and muscles are useful tools to distinguish wild

fish from the fish farming.


O-S2 ǀ Page 68

Geographic variation in life history of ayu species

Iki Murase1, Takahiro Irie2, Kei’ichiro Iguchi1

1 Graduate school of Fisheries and Environmental Sciences, Nagasaki University, Bunky-

cho 1-14, Nagasaki-city, Nagasaki 852-8521, Japan 2 Atmosphere and Ocean Research Institute, The University of Tokyo

Corresponding author: Iki Murase ([email protected])

The ayu, Plecoglossus altivelis, is an annual amphidromous fish, migrating between

coastal marine waters (for juvenile growth) and rivers (for growth and spawning). This

species is distributed from the northern part of Vietnam to the Korean Peninsula and

very common in the Japanese Archipelago. In Japanese populations, spawning occurs

mainly in autumn and larval fishes drift downstream to spend in coastal waters during

winter until the upstream migration in the coming spring. Previous studies reported that

their lifetime schedule of spawning and upstreaming show geographic variations,

reflecting the latitudinal gradient of environmental conditions. However, the earlier

studies are based on a limited amount of data and a finer grained analysis is required for

understanding of the life history variation. In this study, therefore, we analyzed the

starting dates of spawning and upstreaming by collecting the data from 104 rivers

(including spawning dates of ayu from continental coast and subspecies) covering the

entire distribution, mainly derived from literatures. The dataset ranges from 1983 to

2016, but some rivers contain missing year values. As a result, we found a clear

latitudinal trend that adults spawn earlier at higher latitude while upstreaming occurs

earlier at lower latitude. Furthermore, insular subspecies did not significantly deviate

from the continental populations with respect to the regression line between latitude and

spawning date.


O-S2 ǀ Page 69

Temporal and spatial variation and correlation with environmental factors

of fish community in the upper reaches of Lijiang River, China

Yang Ding, Zhiqiang Wu, Zhaojun Zhu

Guangxi University, College of Life Science and Technology, No.100 Daxue East Road, Nanning City, Guangxi Zhuang Autonomous Region, P.R. China

Corresponding author: Zhiqiang Wu ([email protected])

Four investigations for the biodiversity of the species in the upstream Lijiang River were

carried out seasonally from April 2013 to January 2014. The present study focuses on

the upper main stream and four tributaries of the Lijiang River. Seasonal sampling-

investigations were carried out among 19 sampling sites to acquire important

environmental information about water temperature, dissolved oxygen and flow rate,

and the composition of fish species.

A total of 6926 fish samples were collected during investigation. Identification indicted

that they were 51 species belonging to 4 order, 14 families and 40 genera. Non-metric

Multidimensional Scalings ordination (NMDS) was applied to compare fish

communities in the upper reach of Lijiang River. The results showed that there was little

apparent change associated with the seasons, the fish communities were differed

between each sampling site. Difference of stream characteristics was likely to be caused

by anthropogenic activities like human settlements, agriculture activities and

engineering construction, which would result in variation of fish communities. There

was a close relationship between diversity index and environmental factors such as

dissolved oxygen, water temperature, pH, river width and depth. The fish species was

significantly positively related to pH, water temperature, river width and depth, while

being significantly negative correlation to dissolved oxygen. Canonical Correspondence

Analysis (CCA) ordination with the stepwise selection method revealed that 8 of the 9

environmental variables significantly correlated to the fish communities such as

altitude, distance from the convergence of each tributary, stream width, water

temperature, dissolved oxygen, pH, water depth and sediment particle size. Meanwhile,

fish communities at different sites were associated with different environmental

variables in different seasons.


O-S2 ǀ Page 70

Genetic Sex Differences in the Mud Crab (Scylla paramamosain): Evidence

from Sex Specific SNPs, and Differently Expressed Genes and lncRNAs

Hongyu Ma, Xiaolong Yang, Guidong Miao

Shantou University, Daxue Road 243, Shantou City, Guangdong Province, China

Corresponding author: Hongyu Ma ([email protected])

The mud crab (Scylla paramamosain) is a commercially important marine portunid

species in China. Females exhibit higher growth rate and bigger body size than males.

With mature ovary, females are more nutritious and expensive than males also. In order

to identify the genetic difference between males and females, sex specific SNP loci were

isolated using RAD-sequencing, and sex specific or differently expressed genes and

lncRNAs were discriminated using transcriptome-sequencing.

A total of 1.78 million potential SNPs were detected from 20 specimens (10 males and

10 females) using RAD-sequencing. After comparison analysis, 20 SNP loci distributed

among 11 contigs were found to be homogygous in males, but heterozygous in females,

which indicated that these loci are the candidate female specific markers. In order to

confirm if they are sex specific markers, a pair of primers for one SNP locus (70 C>T)

was further designed to amplify and sequence it in additional 95 specimens (42 males

and 53 females). The results showed that not only this SNP locus, but 7 new SNP loci

(62 C>T, 74 C>T, 145 T>C, 147 G>A, 163 C>T, 186 A>T, and 194 T>G) were also

identified and confirmed to be homogygous in males and heterozygous in females.

Hence, a total of 8 SNP markers were confirmed to be female specific in the mud crab.

Moreover, we successfully developed a rapid molecular sexing technique for this crab

species according to the female specific SNP loci.

Transcriptome-sequencing generated 79.2 and 79.8 million reads from ovary and testis

respectively. A total of 287.6 thousand transcripts and 262.6 thousand unigenes were

obtained. 316 unigenes showed significantly differential expression level between ovary

and testis based on the transcriptome data. Further, 20 unigenes were randomly selected

and confirmed by qRT-PCR. Three unigenes were observed to express only in ovary or

in testis, but did not express in both gonads. In addition, a total of 233 thousand putative

lncRNAs were identified, and they were predicted to link to 19,898 target unigenes. In

total, 147 lncRNAs exhibited significantly differential expression level between ovary

and testis based on transcriptome data. Further, the different express of 26 lncRNAs

have been validated by qRT-PCR assay. Three lncRNAs were found to express only in

ovary, and one lncRNA was found to express only in testis. Nine lncRNAs showed a

positive trans-acting effect to their target 8 genes in ovary and testis by qRT-PCR

experiment. All qRT-PCR results showed a good consistency with Illumina sequencing

data in this study.

This work indicated an obvious genetic difference between ovary and testis of the mud

crab (Scylla paramamosain), which will facilitate to eliminate the molecular mechanism

to gonad development and differentiation in crabs.


O-S2 ǀ Page 71

Adaptation to residency in rainbow trout (O. mykiss) above barriers to

migration: Alternative molecular pathways towards a predictable phenotype

Matthew A Campbell

University of California Santa Cruz, PO Box 1181, Palmer AK 99645

Corresponding author: Matthew A Campbell ([email protected])

How organisms maximize fitness in their current environments is a major focus of

biological research. In particular, the identification of links between a phenotype

increasing fitness and a heritable basis. Genetic studies of adaptation are prolific, but

many questions remain. Does adaptation utilize existing variation or new mutations?

Are supergenes major players in most adaptations? Does the same phenotypic change

occur with the same underlying genetic basis? In the frame of these questions from

RADseq data we identify genomic regions under differential selection by comparing the

genomic diversity of four populations of now above-barrier and landlocked rainbow

trout (O. mykiss) with the ancestral diversity present in a below-barrier steelhead

population. Compared to anadromous rainbow trout, the landlocked populations show

altered frequencies of inversions on chromosomes 5 and 20. Numerous genomic regions

outside of the two inversion regions are also identified to be under differential selection,

but there is little concordance among above-barrier populations in specific the genomic

regions identified. Our results indicate standing variation plays a role in the ongoing

adaptation of the landlocked populations and that both super genes and single genes

contribute to adaptation of these populations. While selection acts on above-barrier

populations to favor a resident ecotype universally, the resulting genetic changes are

largely distinct between landlocked populations.


O-S2 ǀ Page 72

Population Genetics of Aerolate Grouper (Epinephelus aerolatus) in

Indo-Malaya Archipelago utilizing mitochondrial cytochrome c oxidase

subunit I (COI) gene

NurFadli1, 2, Zainal A. Muchlisin2, Mohd N. Siti-Azizah1

1 School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia 2 Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, Indonesia

Corresponding author: Nur Fadli ([email protected])

Aerolate grouper (Epinephelus aerolatus) is one of commercially-important fish in

Indo-Malaya Archipelago (IMA). Here, we report the genetic structure of this fish in

IMA using COI gene. The fish samples were collected from fish landing sites across

IMA waters. All obtained sequences were edited and aligned using MEGA 6.06

program. Distinctive haplotypes were determined using DnaSP Version 5.10 software.

Hierarchical molecular variance analysis (AMOVA) and pair wise FST comparisons

were examined using Arlequin Versions 3.1. In total, 207 sequences were generated

from thirteen localities. The read lengths were 633bp lengths where 566 were conserve

sites, 35 sites parsimony informative and 32 singletons. In addition, 50 haplotypes were

produced in this study. The Maximum-Likelihood trees of E. aerolatus were split into

two distinct clades with bootstrap values of 99%. This revealed the heterogenous

structure of aerolate grouper populations within the IMA region.


O-S2 ǀ Page 73

Effect of Salinity Changing on Methionine Content in Tiger Grouper

Juvenile (Epinephelus fuscoguttatus)

Abdullah A. Muhammadar, Dedi. F. Putra, Zainal. A. Muchlisin

Syiah Kuala University/ Aquaculture, Jl. Kuta Inong Balee No 26, Darussalam, Banda

Aceh, Indonesia

Corresponding author: Abdullah A. Muhammadar ([email protected])

The objective of the study was to examine the effect of water salinity changing on

methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). The treatment

is the salinity changing of 22 psu and 32 psu for 24 hours at four intervals; (a) salinity

change at 2 hours interval, (b) salinity change at 3 hours interval, (c) salinity shock at 4

hours interval, and (d) salinity change at 6 hours interval, (e) control without salinity

change (the fish were reared in salinity 32 psu). The salinity was up and down for 24

hours according to the respective treatment. The Anova test showed that the salinity

change gave a significant effect on the methionine content on the fish carcass. The

methionine was changing significantly when the fish subjected to salinity shock

between 2 hours to 4 hours intervals. However, no significant changes of methionine

content when the fish subjected to salinity changes within 6 hours intervals. Thus the

cultured of tiger grouper juvenile in the waters with shock salinity fluctuations every 6

hours has no risk of methionine deficiency.


Page 74

Poster Presentation

Abstracts


P-S1 ǀ Page 75

Morphological description of larvae and juveniles of flatfish

(Pleuronectiformes) collected from Ka Long and Tien Yen estuary,

Northern Viet Nam

Nguyen Ha My1, Chu Hoang Nam2, Tran Duc Hau2, Nguyen Xuan Huan1

1 VNU University of Science, Vietnam 2 Hanoi National University of Education, Vietnam

Corresponding author: Nguyen Ha My ([email protected])

Flatfish (Pleuronectiformes) includes about 570 species in 14 families. Flatfish are

characterized by morphological changes from larvae to juvenile (symmetry to

asymmetry with one eye migrating to the other side). This order consists of 34 species

in Vietnam, and plays a commercial importance for local fisheries. However,

information of early stages of flatfish has been poorly concerned. Based on 137

specimens collected from the bank waters of the Kalong and Tien Yen estuaries in

northern Vietnam from 2013 to 2015, the present study attempted to report first

descriptions of larvae and juveniles for Vietnam. Five species (postlarvae and juveniles)

of five genera and three families were identified and described morphologically, namely

Pseudorhombus arsius, Tephrinectes sp., Brachirus orientalis, Solea ovata and

Cynoglossus sp. There were five species from Kalong estuary, and two species in Tien

Yen estuary. Of these species, only Solea ovata appeared at both estuaries, with a catch

per unit effort of 1.42 individuals/2 minutes towing in Kalong and 20.4 individuals/2

minutes towing in Tien Yen. In other species, the catch per unit effort was less than 1

individuals/2 minutes towing. A detailed description of morphology and identification

key to species were also given in the present study.


P-S1 ǀ Page 76

A new species of sinistral flatfish genus Chascanopsetta (Teleostei: Bothidae)

from Papua New Guinea, western Pacific Ocean

Pakorn Tongboonkua, Mao-Ying Lee, Wei-Jen Chen

Institute of Oceanography, National Taiwan University, No.1, Sec. 4, Roosevelt Road,


Corresponding author: [email protected]

Left-eyed flounders of the genus Chascanopsetta occur in the deep waters at depths

ranging from 160 to 640 meters in the Indian, Pacific and Atlantic Oceans. They are

predators and can bury in sediment to camouflage themselves on the seabed and prevent

current shear. The genus Chascanopsetta can be distinct from other bothid genera by

having a strongly compressed and essentially deep-bodied and by a tremendously large

mouth. Currently, eight species are recognized in Chascanopsetta, and five of them are

distributed in western Pacific. During several biodiversity expeditions in western

Pacific conducted under the Tropical Deep-Sea Benthos program and the cooperation

project between Taiwan and France, TFDeepEvo, we collected Eleven specimens of

Chascanopsetta. Among them, 7 present distinguishable morphological features to

other 5 nominal species occurring in this region, and could belong to an undetermined

species of genus Chascanopsetta. In this study, we carefully examined their morphology

and compare them with the specimens of other congeneric species. The result revealed

that the combination of the following characters can be used as diagnose of this new

species to others: (1) Number of gill-rakers on the first gill arch (14-18 v.s. 0-13). (2)

Counts of anal-fin rays (77-84 v.s. 85-98). (3) Number of caudal vertebrae (36-38 v.s.

40-44). We are currently generating the DNA sequence data from mitochondrial

cytochrome oxidase subunit I (COI) gene and other nuclear gene(s) from the examined

specimens of this potentially new species and other species that are available in our lab

to confirm its taxonomic status and to infer its phylogenetic position within the

Chascanopsetta.


P-S1 ǀ Page 77

The anatomy of the endocrine pancreas among teleosts:

phylogenetic implications

Bruno Chanet1, Wei-Jen Chen2, Nalani Schnell1 and Claude Guintard3

1 ISYEB, UMR7205 MNHN-CNRS-UPMC-EPHE, Muséum national d'Histoire naturelle,

Dpt Systématique et Evolution, CP30, 25 rue Cuvier, 75005 Paris, France 2 Institute of Oceanography, National Taiwan University, Roosevelt Road, Taipei 10617,

Taiwan 3 Laboratoire d’Anatomie Comparée, ONIRIS – Ecole Nationale Vétérinaire de

l’Agroalimentaire et de l’Alimentation, Nantes Atlantique, Route de Gachet, CS 40 706,

44307 Nantes Cedex 03, France.


A survey of the comparative anatomy of the endocrine pancreas on the basis of

literature, dissections and MRI for the specimens across 300 actinopterygian species in

46 orders and 119 families was carried out. The result revealed a wide range of variation.

We observed that: (1) this variation was categorized firstly by the organization of the

organ: diffused versus compact, with few large macroscopic islets, named Brockmann

Bodies (BB) or principal islet, (2) the large endocrine pancreatic nodules have appeared

several times during the evolution of the Teleostei: at least in some Cypriniforms, some

Salmoniforms and the immense majority of the lineages within the Ctenosquamata

(Myctophiformes plus Acantomorpha). Moreover, the presence of a BB in the

Ateleopodiformes may justify a close affinity of this group with the Ctenosquamata

and/or Aulopiformes, which is in agreement with the most recent molecular hypothesis

(Chen et al. 2014; Betancur-R et al., 2013). Within the Acanthomorpha, the absence of

BB is observed in parrotfishes and some of their allies from the Labridae. More

investigations are still needed to corroborate the existence or absence of BB as putative

synapomorphies for these lineages.


P-S1 ǀ Page 78

Molecular Systematics of lizard fishes (Teleostei: Synodontidae):

a preliminary result based on two gene makers

Shih-Yu Wang, Barry C. Russell, Hsuan-Ching Ho, Wei-Jen Chen

Institute of Oceanography, National Taiwan University, Room 303, Institute of

Oceanography, National Taiwan University, No.1 Sec. 4 Roosevelt Rd., Taipei 10617,

Taiwan

Corresponding author: Shih-Yu Wang ([email protected])

The lizardfish family Synodontidae, a member of the order Aulopiformes, contains 81

currently recognized species that are placed in four genera, including Synodus, Saurida,

Harpadon, and Trachinocephalus. They are mostly voracious and carnivorous fishes of

moderate size, living on sandy bottoms (expect Harpadon species which are pelagic) in

tropical shallow and deep waters in the Atlantic, Indian, and Pacific Oceans. Most

species of the Synodontidae family occur in the Indo-West Pacific. However, there are

still some obscurities in the taxonomy (especially from those taxa occurring in the Indo-

West Pacific) and phylogeny of the lizardfish family. Actually, some cryptic species

may exist but has not yet been investigated. Recently developed molecular approaches

based on the variation of DNA sequences are useful to improve the accuracy of species

identification in such kind of circumstances and to study the evolutionary relationships

of the taxa. In this study, multiple-gene analyses based on the sequences presently from

COI and RH genes of the samples will be conducted to reconstruct the phylogeny of the

Synodontidae. The monophyly of each genus and subfamily will be tested according to

the phylogenetic results. The examined samples were collected during several

biodiversity expeditions in western Pacific conducted under the Tropical Deep-Sea

Benthos program and the cooperation project between Taiwan and France, TFDeepEvo,

and from museum collections of the National Museum of Marine Biology and

Aquarium (Taiwan) and the Museum & Art Gallery of the Northern Territory

(Australia). With the compiled dataset of this throughout taxon sampling, we intend also

to examine the validity of species and explore the cryptic diversity of the family.


P-S1 ǀ Page 79

Identification and morphological comparison of Sillaginidae Species in

Southern Coast of Iran by using traditional morphologic characters and

Geometric statistics

Mohammad Sadegh Alavi-Yeganeh

Tarbiat Modares University/ Department of Marine Biology, Nur, Po box: 14155-4838-

Corresponding author: Mohammad Sadegh Alavi-Yeganeh ([email protected])

Identification and Taxonomy of Sillaginid fishes of southern coast of Iran were

investigated by using traditional morphometrics (10 metric and 28 metric characters),

geometrics morphometric methods (20 homologous landmarks) and a molecular marker

(Co1 sequencing). Fish samples were obtained from eight regional fish market from The

Persian Gulf and Oman sea shores (Chabahar, Jask, Bandar abass, Qeshm, Hormoz,

Dayer, Kangan, Bushehr) during summer to winter 2016. A total number of 280

specimens were used for morphological comparison and 17 specimens were used for

molecular analysis. Four species: Sillago sihama (Silver sillago) S. srabica (Arabian

sillago), S. attenuate (Slender sillago) and S. indica (Indian sillago) were identified in

the Iranian coast of the Persian Gulf and Oman Sea based on swim bladder and key

characters. Despite of FAO report of S. chondropus from study area, no specimens were

observed in this study. Also S. indica was reported from Iranian as the first record. The

statistical analysis including Discriminate analysis, Principle component Analysis,

Principle component Analysis and Canonical Variate Analysis of morphological traits

revealed the geometrics morphometry, morphometric and meristic methods respectively

as the most convenient methods of morphological comparison in Sillaginid fishes of

Iran. According to the result, S. sihama can be distinguished from the other species

having the least predorsal length. S. indica has the most body depth and the most caudal

peduncle depth. Despite significant statistical differences of S. attenuatae with other

sillaginid species, there was no diagnostic character for this species. The landmark

based geometric morphometric method revealed some more morphological differences

among species comparing with traditional methods including some caudal peduncle and

body measurement traits.


P-S1 ǀ Page 80

Survey on the species composition of the Perciformes in different habitats in

Con Dao island, Vietnam

Huynh Kim Thanh, Hoang Duc Huy, Jean-Dominique Durand, Pham Manh Hung,

Tran Trong Ngan


Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Vietnam.

Corresponding author: Thanh Huynh Kim ([email protected])

Perciformes has a high diversity with more than 1,614 species recorded in Southeast

Asia. However, the Perciformes in Con Dao Island still does not have sufficient

information on species composition, habitat, only one study by Nguyen Huu Phung and

Nguyen Van Long since 1997. The study “Survey on the species composition of the

Perciformes in different habitats in Con Dao Island, Vietnam” aims to provide a list of

the Perciformes in this area and their habitats. The study period is from January to April

2017 at different coastal habitats in Con Dao Island. Sampling specimen by fishermen

supporting, direct observation by diving and analyzing local ecological knowledge. Fish

identification is based on morphological characteristics. The results of the study

recorded total of 80 fish species, 27 genera, 15 families. Their habitats are coral reefs,

seagrass beds and mangroves. Of these, 56 species living in coral reef; 1 in coral reefs

and seagrasses; 3 species live in seagrass and mangroves; 18 species live in mangrove

and coral reefs; 2 species live in seagrass. For completed fish data to reflect the species

composition of the Perciformes in Con Dao, the study should extend in the rest of

months (July - December).


P-S1 ǀ Page 81

Molecular characterization and phylogenetic relationship of freshwater

barbs of Bangladesh exploring Cytochrome Oxidase Subunit I (COI) gene

Md. Sagir Ahmed, Luthfun Nahar and Nafisa Nawal Islam



A study was conducted to know the molecular characterization, phylogenetic

relationships and the biodiversity status of freshwater barbs of Bangladesh. Samples

were collected from the major rivers, haor, baor, beels and floodplains of north eastern

regions of Bangladesh. Eleven species under three genera of the family of Cyprinidae

were recorded. DNA Barcoding has been considered as a global bio-identification

system for freshwater and marine fishes in recent years. In the present study, all species

of barbs were investigated using both morphological and molecular genetic analyses

using mitochondrial Cytochrome c Oxidase subunit 1 (COI) gene. The average Kimura

two parameter (K2P) distances within species and genera were 0.39%, 15.80%,

respectively. In addition to barcode-based species identification system, phylogenetic

relationships among the species have also been attempted. The neighbour-joining tree

revealed distinct clusters in concurrence with the taxonomic status of the species.


P-S1 ǀ Page 82

Phylogenetic evolution of global marine angelfishes (Pomacanthidae):

a coordinated study by molecular phylogeny and otolith morphometry

Kang-Ning Shen1, Chen-Yi Lee2, Chih-Wei Chang2,3

1 Aquatic Technology Laboratories, Agricultural Technology Research Institute, Hsinchu

300, Taiwan ROC 2 Department of Exhibition, National Museum of Marine Biology and Aquarium, Pingtung

944, Taiwan ROC 3 Graduate Institute of Marine Biology, National Dong Hwa University, Hualien 974,

Taiwan ROC

Corresponding author: Chih-Wei Chang ([email protected])

Fishes of the Pomacanthidae, commonly known as marine angelfishes, widely

distributed in coral reefs of the tropical Atlantic, Indian, and Pacific Oceans. There are

at least 8 genera and 82 species of pomacanthids in the world and 6 genera and 29

species in Taiwan. Species numbers of pomacanthids are relatively few in comparison

with other coral reef fish families. However, the interspecific variations in the

zoogeographic range, fish shape, and size enable pomacanthids the adequate model for

phylogeny studied by both molecular (genetic) and otolith morphological (phenotypic)

approaches. With the advantage of having the greatest species numbers of preserved

pomacanthid specimens, the study particularly aims to the evaluations and debates such

as monophyly of Centropyge and Holacanthus, validity of Paracentropyge, and

systematics of Pygoplites and Apolemichthys arcuatus. Results indicated that

Chaetodontoplus is the most ancestral genera in Pomacanthidae; Pomacanthus comes

the second and all other genus cluster in a big clade with very low bootstraps support.

For otoliths, Pomacanthidae is composed of clades based on otolith circularity, while

Genicanthus shows a relative consistence in otolith morphology. Centropyge and

Genicanthus share similar otolith morphological parameters, whereas the other genus

reveal multiple clades. Several species of Pomacanthidae still have an ambiguous

position in comparison to the congeneric species based on otolith morphology, e.g.,

Centropyge venusta, C. interruptus, Genicanthus semifasciatus, and Pomacanthus

zonipectus.


P-S1 ǀ Page 83

Identifying fish larvae by DNA barcoding approach at

Con Dao Island, Vietnam

Vo Thi Bich Thao, Pham Manh Hung, Jean-Dominique Durand, Hoang Duc Huy,

Tran Trong Ngan


Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Vietnam

Corresponding author: Thao Thi Bich Vo ([email protected])

Information on composition of fish larvae is basis for understanding early life cycles of

fishes that is necessary for establishing the marine protected areas, particularly for Con

Dao Island (the oldest Vietnamese MPA) due to limited database up to now. The study

“Identifying fish larvae by DNA barcoding approach at Con Dao Island, Vietnam” is

conducted with main objective: identifying fish larvae to the species level by DNA

barcoding approach collected in three different habitats: seagrass beds, coral reefs and

sandy areas. Sampling specimen from June to July 2016 in three habitats divided into

five locations. Fish larvae are collected by light traps at night, then divided into similar

morphology groups by morphology method and identified by DNA barcoding approach.

Total of 419 sequences, including 349 sequences were identified to the species level

(83.3%): Hypoatherina temminckii, Hypoatherina panatela, Harpadon nehereus,

Saurida argentea, Saurida macrolepis, Saurida micropectoralis, Saurida tumbil,

Opisthonema oglinum, Sardinella jussieu, Spratelloides delicatulus, Spratelloides

gracilis, Encrasicholina heteroloba, Encrasicholina punctifer, Stolephorus indicus,

Gerres filamentosus, Gerres japonicus, Gerres oyena, Equulites leuciscus, Secutor

ruconius, Sillago sihama, Rhynchopelates oxyrhynchus, Lepturacanthus savala; 5

sequences were identified to the genus level (1.2%): Sardinella sp., Encrasicholina sp.,

Bregmaceros sp.; 65 sequences were identified to the family level (15.5%): Atherinidae

sp., Clupeidae sp., Gerreidae sp., Gobiidae sp.. The study records 22 species belong to

11 families, 6 orders of fish larvae in three habitats. Seagrass beds (17 species) have the

highest species diversity include: 5 species belong to Synodontidae, 5 species belong to

Clupeidae, 2 species belong to Engraulidae, 2 species belong to Gerreidae, 2 species

belong to Leiognathidae, 1 species belong to Sillaginidae, 1 species belong to

Trichiuridae. Coral reefs (12 species) include: 2 species belong to Atherinidae, 3 species

belong to Clupeidae, 3 species belong to Engraulidae, 1 species belong to Gerreidae, 2

species belong to Leiognathidae, 1 species belong to Terapontidae. Sandy areas (3

species) include: 2 species belong to Clupeidae, 1 species belong to Gerreidae.

Perciformes have the most diverse family, Clupeidae (Clupeiformes) have the most

diverse genus, Saurida (Synodontidae) have the most diverse species. Fish larvae

identification by DNA barcoding approach to the species level have high accuracy.

DNA barcoding approach is essential for identifying fish larvae to provide information

on composition of fish larvae for Con Dao Island. For specimens were not identified to

the species level, it need to combinate with study on adult fishes by molecular approach

and compare with morphology of fish larvae. The study on fish larvae composition in

three habitats: seagrass beds, coral reefs and sandy areas monthly is necessary at Con

Dao Island in the next time.


P-S1 ǀ Page 84

Asymmestry otoliths of Sillago sihama (FORSSKAL, 1775) in

some areas of Vietnam

Ta Thi Thuy1, To Thuy Dung2, Tran Duc Hau2 1Hanoi Metropolitan University

2Hanoi National University of Education

Corresponding author: To Thuy Dung; email: [email protected]

Otolith is a structure in the inner ear of all bony fish. This structure plays a role as a

balance system and contributes to fish hearing. Each otolith comprises 3 pair-structures

of sagittae, lapilli and asterisci, which are different in shape, size and location in

semicircular canals. There are usually an asymmetry between the left and the right side

of each pair. To examine the asymmetry of otolith structure in Sillago sihama

(Sillaginidae) from different geographic locations, we collected samples at the Ka Long

and Tien Yen Rivers in Quảng Ninh province; the So River in Nam Dinh Province and

Danang City. From 287 specimens of Sillago sihama collected from the four areas, a

total of 533 sagittae, 180 lapilli, 69 asterisci were removed. Morphological examination

indicated that there was an asymmetry of otolith shape among two sides, but varied

between rivers. The asymmetry occurred clearly at 9.9-18.5 mm body length for the

Kalong, 11.7-17.3 mm for the Tien Yen but unclearly for the so samples. For the otolith

size, Kalong and Tien Yen samples showed a similarity until juvenile stage but became

different in mature fish. Importantly, otolith size of this species revealed an asymmetry

for the mature fish collected in Danang. Thus, the present study indicate that variation

in otolith morphology could be found between right and left sides and among

geographical locations, reminding a further consideration in using otolith morphology

as an identification character in this species. Also, morphology of otolith was given in

the present study.


P-S2 ǀ Page 85

Population mixing of Pacific bluefin tuna (Thunnus orientalis) revealed by

otoliths isotope analysis

Cheng, Ching-Chun, Shiao, Jen-Chieh

Institution of Oceanography, National Taiwan University


Pacific bluefin tuna (Thunnus orientalis; PBF) have two spawning grounds, one in the

western Pacific Ocean ranging from the Philippines Sea to the Okinawa and the other

one located in southeastern area of the Sea of Japan. According to our previous study,

PBF hatched in each spawning ground can be determined by otolith δ18O profile, which

represents the water temperature experienced by the fish. The PBF hatched in the Sea

of Japan experienced the lower winter water temperature at earlier age, which created

the first δ18O peak around 800-1000 μm from the core. In contrast, the PBF hatched in

the western Pacific Ocean experienced the winter temperature at later age and recorded

the first δ18O peak around 1300-1500 μm from the core. Sagittal otoliths of 49 PBF

caught in the western Pacific Ocean and 13 PBF caught in the Sea of Japan were

analyzed by the isotope ratio mass spectrometry. The sequential variations of otolith

δ18O compositions suggested that most PBF caught in the northwest Pacific Ocean were

hatched in the same spawning ground (92%, n = 45) while only a small portion (8%, n

= 4) were hatched in the Sea of Japan. For the PBF caught in the Sea of Japan, 62%

were hatched in the Pacific Ocean while 38% of the fish were hatched in the Sea of

Japan. The results suggested that Pacific spawning grounds have greater contribution to

the stock biomass of the PBF. PBF hatched in the Sea of Japan showed higher fidelity

to the natal spawning ground. The PBF shall be managed as a signal population because

the PBF with different natal origins mixed in both spawning grounds, indicating

sufficient genetic flow.


P-S2 ǀ Page 86

Fast identification of Forbidden shark species by insulated isothermal PCR,

iiPCR technique

Han Shang Jung, Jen-Chieh Shiao

National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan Institute

of Oceanography Room 426

Corresponding author: Han Shang Jung ([email protected])

Identification of shark species at landing has been difficult because most key diagnostic

features have been removed at catch. Molecular approaches such as DNA barcoding are

able to identify the species without any morphological characters but this approach takes

several days and have to be done in a laboratory with expensive instruments. To shorten

the process of species identification, two species-specific TaqMan probes

complementary to mitochondrial ND2 gene were designed to identify silky shark

(Carcharhinus falciformis) and oceanic whitetip shark (Carcharhinus longimanus)

using a PCR assay performed in capillary tubes under insulated isothermal conditions

(iiPCR assay). After the extraction of sharks DNA by a simple DNA extraction kit,

DNA amplicons were reproducibly generated for 30 min in an iiPCR portable device

(Micro POCKITTM) in which a special polycarbonate capillary tube (R-tubeTM) was

heated isothermally by a copper ring attached to its bottom and shielded by a thermal

baffle around its upper half. Both probes were mixed with the same primer pairs in a

signal reagent, which can detect silky shark and oceanic whitetip shark at the same time.

A total of 29 samples from 6 species were tested, including Sphyrna lewini, Prionace

glauca, Alopias superciliosus, Alopias pelagicus and two target species of

Carcharhinus falciformis and C. longimanus. The results are very promising that 20

samples of targeted species and nine samples of non-targeted species were all correctly

distinguished by the iiPCR assay, with efficiency achieved 100%. The iiPCR assay

developed in this study can distinguish target forbidden shark species from others and

provided a rapid method to identify forbidden shark species.


P-S2 ǀ Page 87

Comparisons on biological characteristics of Topmouth gudgeon of

the invasion and original areas in China

F. Lin1, 2, L. Q. Fan3, F. M. Lin1, Q. H. Zhou1, J. Zhang2

1Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin541004, P. R. China.

2Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China.

3Institute of Plateau Ecology, Tibet Agricultural and Animal Husbandry College, No. 8

Xueyuan Street,Bayi, Linzhi, 860000, P. R. Chinayang

Topmouth gudgeon, Pseudorasbora parva (Temminck and Schlegel, 1846) is a small

cyprinid species that rarely achieves lengths above 80 mm and native to East Asia. Their

high phenotypic plasticity in fitness related life history traits such as rapid growth, early

maturity, fecundity, batch spawning, nest guarding and broad environmental tolerance

limits has been classified as an international non-native species with the ability to

establish sustainable populations following introduction into novel environments. P.

parva has been proven to expand its range, spreading from its native area to more 32

countries in less than 50 years, presenting a potential risk to native ichthyofaunal

through the adverse effects of inter-specific competition, facultative parasitism,

pathogen transfer and disruption of ecosystem functioning. In this study, samples of P.

parva from 13 populations, which including Tibet, Yunnan, Xinjiang, Beijing, Tianjing,

Wuhan, Guilin and so on, were collected in China from 2016 to 2017. A total of 3671

individuals were analyzed for the parameters of length and weight relationships (LWRs)

and Von-Bertalanffy growth equations to estimate the maximum body length, growth

and mortality parameters. The coefficient b of LWRs ranged from 2.95 to 3.34, and the

estimated Von-Bertalanffy growth function parameters were 81.38~112.88mm(L∞),

0.33~0.52yr-1(k), -0.38~-0.63yr-1(t0) respectively as were calculated with ELEFAN

method in FiSAT computer package. Total mortality coefficient (Z) ranged from 1.02

to 2.77 were estimated by transformed body length catch curve method and natural

mortality coefficient (M) ranged between 1.02 and 2.77 were calculated with Pauly

equation. The invasive populations usually showed a slower growth, smaller size,

prolonged longevity comparing with native populations. Furthermore, multivariate

statistics (Procrustean analysis and canonical correlation analysis) identified a strong,

range-wide association between environmental variables (Latitude and longitude,

annual temperature, altitude, average water temperature, flow velocity, conductivity,

Dissolved oxygen, food abundance and so on) and life history parameters. The different

adaptation mechanisms of the invasive populations were also discussed.


P-S2 ǀ Page 88

The study of changes in size, age, sex ratio and otolith sampling methods of

Pacific Bluefin tuna (Thunnus orientalis) caught in

the northwestern Pacific Ocean

Jhen Hsu, Jen-Chieh Shiao, Yi-Jay Chang

Institute of Oceanography, National Taiwan University

Corresponding author: Jen-Chieh Shiao ([email protected])

Pacific bluefin tuna (Thunnus orientalis, PBF), a high-valued species, is heavily

exploited and its biomass reached the historically low level. This study examined the

demographic changes of the PBF in 2002 to 2016 and the outcomes may improve the

management of this species. PBF smaller than 200 cm were rarely found in the tropical

areas (south of 24°N) and the mean sizes of the PBF caught in tropical areas were

significantly larger than those caught in the subtropical areas (north of 24°N, t-test , p

< 0.001). These results suggested that the choice of spawning latitudes of the PBF was

influenced by the size of spawning adults. The sex ratio of smaller (< 200 cm) and

intermediate size classes (200-230 cm) was female dominated (50-75%) while larger

fish (> 240 cm) were male dominated (62-100%). A classical Age-Length Key (ALK)

based on the ages and lengths of 892 fish were applied to the length frequencies of the

catches in the northwestern Pacific spawning grounds to estimate the annual age

compositions of the PBF. Ages of the spawners concentrated in 7-20 years old (range =

4-28 years), corresponding to fork lengths between 200 and 240 cm. In 2013 to 2015,

2005-2009 year-cohorts (age 6-10) increased in relative abundance while the strong

1994 and 1996 year-cohorts decreased because they were near the end of their life span.

We further evaluated the efficiency of three otolith sampling methods: the random

otolith sampling method (ROS), the equal otolith sampling method (EOS) and the

reweighting otolith sampling (REW) by simulating approach using different sample

size. The uncertain reduced as the sample size increased for all three methods, however,

the REW provides higher efficiency than the ROS and EOS methods. Very little

uncertainty can be reduced when the sample size increase to 500, which might be the

minimum effective otolith sample sizes. The REW methods and 500 otoliths can be

regarded as a guild line for the future study and management.


P-S2 ǀ Page 89

Comparative study the expression of calcium cycling genes in Bombay duck

(Harpadon nehereus) and beltfish (Trichiurus lepturus) with

different swimming activities

Hui ZHANG1,2, Weiwei XIAN1,2

1 Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology,

Chinese Academy of Sciences, Qingdao 266071, China 2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory

for Marine Science and Technology, Qingdao 266071, China

Corresponding author: Weiwei XIAN ([email protected])

The contraction and relaxation events of the muscle is mediated by the coordination of

many important calcium cycling proteins of ryanodine receptor (RYR), troponin C

(TNNC), parvalbumin (PVALB), sarcoendoplasmic reticulum calcium transport

ATPase (SERCA) and calsequestrin (CASQ). In higher vertebrates, the expression level

of calcium cycling proteins are positively correlated to the muscle

contraction/relaxation ability of the cell. In this study, we used RNAseq to explore the

expression profile of calcium cycling genes between two marine fish of Bombay duck

(Harpadon nehereus) and beltfish (Trichiurus lepturus) with poor and robust swimming

activities, respectively. We have studied the hypothesis whether the expression level of

calcium cycling proteins are also positive correlated to swimming ability in fish. We

used Illumina sequencing technology (NextSeq500) to sequence, assemble and annotate

the muscle transcriptome of Bombay duck for the first time. A total of 47,752,240

cleaned reads (deposited in NCBI SRA database with accession number of

SRX1706379) were obtained from RNA sequencing and 26,288 unigenes (with N50 of

486 bp) were obtained after de novo assembling with Trinity software. BLASTX against

NR, GO, KEGG and eggNOG databases show 100%, 65%, 26%, 94% and 88%

annotation rate, respectively. Comparison of the dominantly expressed unigenes in fish

muscle shows calcium cycling gene expression in beltfish (SRX1674471) is 1.4- to

51.6-fold higher than Bombay duck. Among five calcium cycling genes, the fold change

results are very significant in CASQ (51.6 fold) and PVALB (9.1 fold) and both of them

are responsive for calcium binding to reduce free calcium concentration in the

sarcoendoplasmic reticulum and cytoplasm. In conclusion, we confirmed that the high

abundant expression rate of calciumcycling genes in robust swimming fish species. The

current muscle transcriptome and identified calcium cycling gene data can provide more

insights into the muscle physiology of fish.


P-S2 ǀ Page 90

Bangladesh Barcode of Life (BdBOL)

Md. Sagir Ahmed



Bangladesh having an area of 147,570 sq km, contains 251 inland fishes, 402 marine

fishes, 34 amphibians, 126 inland reptiles, 17 marine reptiles, 388 resident birds, 300

migratory birds, 110 inland mammals and 03 marine mammals. This is really an

extraordinary situation that such a great diversity still exists in an unusually

overpopulated country. Unfortunately, there is no database and regular monitoring

system exists for this invaluable resources. BdBOL (Bangladesh Barcode of Life) is an

Association that aims at establishing a central coordination of the activities related to

the DNA barcodes in Bangladesh. The BdBOL network (bdbol.net) was founded with

the goal of using DNA barcoding to capture the diversity of life in Bangladesh and to

use this information to monitor national biodiversity and enhance conservation

strategies. The association BdBOL has as main goal to promote the use of genetic

methods in the study and monitoring of Bangladesh biodiversity through: the

collaboration and the exchange of professional experiences; the coordination of the

national initiatives; the creation of a network of expertise in the field of DNA

sequencing and of its scientific and practical application; the support of its members on

fund raising; the taking into account of its members interests in matter of management

of the genetic resources; establish relationships with international institutions active in

the same domain. Our vision is to assemble a comprehensive library of standardized

DNA sequences (DNA barcodes) as a reference resource for research and management

of biodiversity in Bangladesh. We will also promote international collaboration for

DNA barcoding of biodiversity.


P-S2 ǀ Page 91

Population genetic structure of the rock bream; Oplegnathus fasciatus

based on the SSR

Yongshuang Xiao1, Jun Li

Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao,

Shandong 266071, China

Corresponding author: Jun Li ([email protected])

As a rocky reef species, Oplegnathus fasciatus displays significant geographical

differences that may be congruent with patterns of molecular variation. We used ten

polymorphism microsatellite loci to characterize the genetic structure and test the

impact of ocean currents and the Yangtze River on the population genetic connectivity

for the rock bream, O. fasciatus, sampled from Jiaonan (JN), Zhoushan (ZS) and Taiwan

(TW). The average number of alleles was 17.78, with a total of 160 alleles, and the

average allele value in the East China Sea (ZS = 13.70) was higher than that in the

Yellow Sea (JN = 6.70). The individuals at the ZS location showed the highest expected

heterozygosity (He = 0.86), Nei’s genetic diversity (H = 0.84) and Shannon genetic

diversity (I = 2.21). Genetic bottleneck tests showed that individuals had experienced a

recent bottleneck effect in the JN location. AMOVA analysis showed that 12.19% of

genetic variation existed among populations, indicating significant population genetic

differentiation in the species. The values of population pairwise differentiations index

(Fstand Φpt) ranged from 0.01 to 0.29. The UPGMA tree showed two diverged

genealogical branches corresponding to sampling localities (Clade A=JN, Clade B= ZS

and TW) in the species. The AMOVA and STRUCTURE analyses suggested that O.

fasciatus may be composed of 2 stocks in the examined range, and the freshwater

discharge from the Yangtze River might act as a physical barrier to genetic exchange

between north and south China stocks of the species.


P-S2 ǀ Page 92

DNA Barcoding of the Japanese scad, Decapterus maruadsi (Temminck &

Schlegel, 1843) in South China Sea and neighbouring waters

Danial Hariz Zainal Abidin, Noorul Azliana Jamaludin, Wahidah Mohd Arshaad,

Nguyen Viet Nghia, Siti Azizah Mohd Nor, and Noor Adelyna Mohammed Akib

Centre for Global Sustainability Studies (CGSS), University Sains Malaysia, 11800,

Penang, Malaysia


DNA barcoding of the cytochrome oxidase subunit I (COI) gene was utilized to assess

the phylogenetic relationships of the Japanese scad, Decapterus maruadsi among nine

populations within the South China Sea and neighbouring waters. Here, a 651 bp region

of COI was sequenced for 142 individuals sampled from populations representing South

China Sea, Java Sea, Andaman Sea and Streets of Malacca. Phylogenetic analysis of the

region formed two clades of D. maruadsi samples from northern Vietnam (Nghe An

and Cat Ba) that showed high genetic divergence (0.3% to 2.6%) with the Cat Ba

(Vietnam) population, being the most divergent from all the other populations.

Meanwhile, samples from central Vietnam (Khanh Hoa) formed an admixed population

with South China Sea and neighbouring waters. Therefore, further phylogenetic analysis

was performed with the sampled Decapterus maruadsi and other Decapterus spp.

sequences available on BOLD to confirm the presence of cryptic species among the

studied individuals. The present study exhibited the power of DNA barcoding in

assessing phylogenetic relationships among D. maruadsi samples from populations

within the South China Sea and neighbouring waters.


Page 93

PARTICIPANT LIST

Australia

Barry Russell [email protected] Northern Territory Museum

Bangladesh

Md. Sagir Ahmed [email protected] Department of Zoology

China

Chunlong Liu [email protected] Institute of Oceanology, Chinese Academy of Sciences

E Zhang [email protected] Institute of Hydrobiology, Chinese Academy of Sciences

Feng Lin [email protected] Institute oceanography National Taiwan University

Guangping Cheng [email protected] Guangxi University , College of Animal Science and Technology

Hairong Luo [email protected] South China Sea Institute of Oceanology, Chinese Academy of ciences

Hongyu Ma [email protected] Shantou University, China

Huang Wen [email protected] School of Marine Sciences,Guangxi University

Hui Zhang [email protected] Institute of Oceanology Chinese Academy of Sciences

Jinqing Huang [email protected] Guangxi University, College of Life Science and Technology

Jun Li [email protected] Institute of Oceanology, Chinese Academy of Sciences

Liang Cao [email protected] Institute of Hydrobiology, Chinese Academy of Sciences

Man Zhang [email protected] Guangxi University, College of Animal Science and Technology

Shan Li [email protected] Natural History Research Center, Shanghai Natural History Museum

Wang Yinghui [email protected] School of Marine Sciences,Guangxi University

Wei Zhou [email protected] Southwest Forestry University/Faculty of Forestry

Weiwei XIAN [email protected] Institute of Oceanology, Chinese Academy of Sciences


Page 94

Wenhong Li [email protected] Guangxi University , College of Animal Science and Technology

Xiao-Yu Kong [email protected] South China Sea Institute of Oceanology, Chinese Academy of Sciences

Xu Li [email protected] Southwest Forestry University

Xue-Lin Song [email protected] Institute of Hydrobiology, Chinese Academy of Sciences

Yahui Zhao [email protected] Institute of Zoology, Chinese Academy of Sciences

Yang Ding [email protected] Guangxi University, College of Life Science and Technology

Yang Jian [email protected] School of Environment and Life Sciences, Guangxi Teachers Education University

Yongshuang Xiao [email protected] Institute of Oceanology Chinese Academy of Sciences

Yuanzhou Xiao [email protected] Shanghai Ocean University

Zhiqiang Wu [email protected] Guangxi University

France

Jacques Panfili [email protected] IRD / UMR MARBEC

Jean-Dominique Durand [email protected] IRD (Institut de recherche pour le développement)

India

Yumnam Lokeshwor Singh [email protected] Department of Zoology, University of Science & Technology Meghalaya

Indonesia

Derita Yulianto [email protected]

Syiah Kuala University/ Doctoral Program in Mathematics and Sciences Application (DMAS)

Abdullah. A. Muhammadar [email protected] Syiah Kuala University/ Aquaculture

Agung Setia Batubara [email protected]

Syiah Kuala University, Doctoral Program in Mathematics and Sciences Application (DMAS), Graduate Program

Zainal Abidin Muchlisin [email protected] Syiah Kuala University


Page 95

Iran

Javad Ghasemzadeh [email protected] Chabahar Maritime University- Faculty of Marine Sciences- Iran

Mohammad Sadegh Alavi-Yeganeh

[email protected] Department of Marine Biology, Tarbiat Modares University, Mazandaran

Japan

Daisuke Hoshizaki [email protected] Graduate School of Fisheries and Environmental Sciences, Nagasaki University

Harutaka Hata [email protected] United Graduate School of Agricultural Sciences, Kagoshima University

Hiroyuki Motomura [email protected] The Kagoshima University Museum

Iki Murase [email protected] Graduate school of Fisheries and Environmental Sciences, Nagasaki University

Kei'ichiro Iguchi [email protected] Graduate School of Fisheries Science and Environmental Studies

Ryohei Miki [email protected] Nobeoka Marine Science Station, University of Miyazaki

Malaysia

Abu Hena Mustafa Kamal [email protected] Universiti Putra Malaysia

Amirrudin B. Ahmad [email protected] School of Marine and Environmental Sciences

Danial Hariz Zainal Abidin [email protected]

CENTRE FOR GLOBAL SUSTAINABILITY STUDIES (CGSS), UNIVERSITI SAINS MALAYSIA

Jamsari Amirul Firdaus Jamaluddin

[email protected] Universiti Sains Malaysia

Lim Hong-Chiun [email protected] Universiti Sains Malaysia

Mohamad Aqmal Bin Mohd Naser

[email protected] Universiti Malaysia Terengganu (UMT)

Noorul Azliana Binti Jamaludin

[email protected]

Southeast Asia Fisheries Development Center (SEAFDEC)/Marine Fishery Resources and Management Department (MFRDMD)

Nur Fadli [email protected] School of Biological Sciences

Seah Ying Giat [email protected] Universiti Malaysia Terengganu


Page 96

Siti Azizah Mohd Nor [email protected] Universiti Sains Malaysia

Wahidah Mohd Arshaad [email protected] Marine Fishery Resources Development and Management Department

Pakistan

Javaid Ayub [email protected] former Director Fisheries and wildlife

Nuzhat Shafi [email protected] Department of Zoology, University of Azad Jammu and Kashmir (UAJK)

Singapore

Jie Zhang [email protected]

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences

Kenny Chua Wei Jie [email protected] National University of Singapore/Department of Biological Sciences

Tan Heok Hui [email protected] National University of Singapore

Sri Lanka

Hettiarachchige Priyanga Sajeewanie Jayapala

[email protected] Ocean University of Sri Lanka

Taiwan

Cheng, Ching-Chun [email protected] Taiwan Institute of Oceanography

Fan Li [email protected] Department of Oceanography, National Sun Yat-Sen University

Han Shang Jung [email protected] Taiwan Institute of Oceanography

I-Shiung Chen [email protected] Institute of Marine Biology, National Taiwan Ocean University

Jen-Chieh Shiao [email protected] Institute of Oceanography, National Taiwan University

Jhen Hsu [email protected] National Taiwan University

Kang-Ning Shen [email protected] National Taiwan Ocean University

Keita Koeda [email protected] National Museum of Marine Biology & Aquarium

Mao-Ying Lee [email protected] Institute of Oceanography, National Taiwan University

Nian-Hong Jang-Liaw [email protected] Taipei Zoo


Page 97

Pakorn Tongboonkua [email protected] National Taiwan University , Taipei · Institute of Oceanography

Shih-Yu Wang [email protected] National Taiwan University , Taipei · Institute of Oceanography

Wei-Jen Chen [email protected] Institute of Oceanography, National Taiwan University

Thailand

Chaiwut Grudpan [email protected] Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University

Jarungjit Grudpan [email protected] Department of Fisheries, Faculty of Agriculture, Ubon Ratchathani University

U.S.A

Lawrence M. Page [email protected] Florida Museum of Natural History

Matthew A Campbell [email protected] University of California Santa Cruz

Zachary Randall [email protected] Florida Museum of Natural History

Viet Nam

Chu Hoang Nam [email protected] Hanoi National University of Education, Vietnam

Dinh Minh Quang [email protected] Can Tho University

Hoang Kim Duyen [email protected] Hanoi National University of Education

Hoang Duc Huy [email protected] University of Science, Vietnam National University, Ho Chi Minh city

Huynh Kim Thanh [email protected] University of Science, Vietnam National University, Ho Chi Minh city

Nguyen Hoang Xuan Anh [email protected] Biology Department, Hanoi National University of Education

Pham Manh Hung [email protected] University of Science, Vietnam National University, Ho Chi Minh city

Ta Thi Thuy [email protected] Faculty of Natural Science, Hanoi Metropolitan University

To Thuy Dung [email protected] Ha Noi University of Education, Ha Noi, Vietnam


Page 98

Tran Duc Hau [email protected]

Department of Zoology, Faculty of Biology, Hanoi National University of Education

Tran Thi Viet Thanh [email protected]

Vietnam National Museum of Nature/Department of Experimental Taxonomy and Genetic Diversity

Tran Trong Ngan [email protected] University of Science, Vietnam National University, Ho Chi Minh city

Vo Thi Bich Thao [email protected] University of Science, Vietnam National University, Ho Chi Minh city

Dang Thuy Binh [email protected] Institute for Biotechnology and Environment, Nha Trang University

Designed by Vo Ngoc Thinh, Le Minh Phong, Pham Manh Hung

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