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1 CODIS Database for the Identification of Missing Persons and Unidentified Human Remains CODIS Database for the Identification of Missing Persons and Unidentified Human Remains John E.B. Stewart, Ph.D. National Missing Person DNA Database Program FBI Laboratory Quantico, VA COmbined DNA Index System Convicted Offenders Forensics Missing Persons CODIS Mission - to provide a means for crime laboratories to electronically compare DNA profiles, thereby linking crimes to each other and to convicted offenders Population Statistics

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CODIS Database for the Identification of Missing Persons and Unidentified Human Remains

CODIS Database for the Identification of Missing Persons and Unidentified Human Remains

John E.B. Stewart, Ph.D.National Missing Person DNA Database ProgramFBI LaboratoryQuantico, VA

COmbined DNA Index System

Convicted OffendersForensics

Missing Persons

CODIS Mission - to provide a means for crime laboratories to electronically compare DNA profiles, thereby linking crimes to each other and to convicted offenders

gPopulation Statistics

2

NDIS

CODIS Architecture

LDISGarland, TX

LDISEl Paso, TX

SDIS FDLECalDOJ

LDISLubbock, TX

DPS

CODIS Indexes

ForensicConvictedOffender Missing Persons

• Unidentified Human Remains

• Missing Person• Relatives of Missing

Person• Spouse

Recently AddedArresteeLegal

COMBINED DNA INDEX SYSTEM (CODIS)

Category Count (Through May 2008)

Investigations Aided 70,511

Forensic Hits 12,786

Offender Hits Within State 49,983

Offender Hits3 Inter State 7 400Offender Hits Inter State 7,400

NATIONAL DNA INDEX SYSTEM (NDIS)

Category Count (Through May 2008)

Convicted Offender 5,806,292

Forensic 222,199

Arrestees 119,222

Legal 288

Missing Person 517

Relatives of Missing Person 4,429

Unidentified Human Remains 1,637

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Convicted Offender Index

Contains DNA profiles of individuals convicted of felony sex offenses (and other violent crimes)

Th i DNA fil iThe crime scene DNA profile isalso searched against the offender index

Matches in the offender index provide investigators with known suspects

Arrestee

An Arrestee Index consists of DNA records of persons who have been arrested or indicted or charged in an information with a crime and are required by law to provide DNA samples.

LegalThe known reference sample from a person whose DNA sample is collected under applicable legal authorities, provided that DNA samples that are voluntarily submitted solely for elimination purposes shall not qualify as a Legal specimen. An example of a Legal specimen is a sample collected from a person found not guilty by reason of insanity who is required by the relevant state law to provide a DNA sample for analysis and entry into a state DNA database. The DNA profile for this specimen category is stored in a Legal Index.

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INDEXMinimum number of

STR’smtDNA

Convicted Offender STR 13Forensic STR 10Unidentified Human 8

NDIS Searches

Unidentified Human Remains 8

and Amelogenin

HV1 & HV2

Missing Person 8and

AmelogeninHV1 & HV2

Biological Relatives of Missing Persons 13

and Amelogenin

HV1 & HV2

Investigations Aided Through May 2008

70,511 Investigations Aided in 49 States and 2 Federal Laboratories

237

685 (CT)

9(DE)

61

(NJ)

(MA)(NH)

877

2,0222,158

4,3171,011

2,680 (RI)6

0 (VT)550

1,9027

2561

6,188

2,1584,3171,0116 957

2,6801,384

8

49

1,050

16 342558

30

19

The “Investigations Aided” is a metric that tracks the number of criminal investigations where CODIS has added value to the investigative process.

2,101

9(DE)

250 (FBI)

42 (US Army)

998 (MD)

825

17

7,816 (FL)

(Hawaii)27

6,531

214

2,119 432

860 4,659825

1,504

6,957

345

206

49

35

2,428

698

674

215

2,433

744

1,404

177 Federal, State and Local CODIS Laboratories

Forensic HitsThrough May 2008

12,786 Forensic Hits in 49 States and 2 Federal Laboratories

57

3341

6

146

120 (CT)

28

(NJ)

(MA)318(NH)22

197

2,127

507

4671931,128

361157

1

9

125

1368

20

103

5

0 (VT)

(RI)14

151

25

53

447 25

146 4 (DE)

104 (FBI)

13 (US Army)

301 (MD)359

157431

4

1,850 (FL)

(Hawaii)13

, 8

123

17

7

632

374

222

11

370

204

258

5

Offender HitsState DNA Index System (SDIS)Through May 2008

49,983 Offender Hits in 47 States and 2 Federal Laboratories

413

1,2212

16

411 (CT)

0

88

(NJ)

(MA)(NH)

5159

1,341

4,072

1,2973,264655

4 0

2,2331,138

12

728

3205

3

235

170 (RI)

(VT)0

5,701

1,562

151

1,344 349

6370 (DE)

34 (FBI)

6 (US Army)

548 (MD)3,988461

1,123

3

5,549 (FL)

(Hawaii)29

4,750

118

145

27

6

1,688

412

320

149

1,720

439

846

Offender HitsNational DNA Index System (NDIS) Through May 2008

7,400 National Offender Hits in 49 States and 2 Federal Laboratories

17187

1978

12

68 (CT)(NJ)

(MA)(NH)

999

335

306

244228200

223192

9

147

1073

5

145

99 (RI)

(VT)0

403

183

19

204 82

863 (DE)

283 (FBI)

33 (US Army)

97 (MD)408140

172

10

883 (FL)

(Hawaii)4

200314

73

111

36

27

246

75

121

91

212

98

254

The Unidentified Human Remains IndexSkeletal/Soft Tissue/Blood

Missing Person IndexSamples from Missing Persons

Deduced Missing Person Samples

The Relatives of Missing Persons Index

Biological Relatives of Missing Persons

The Spouse Index

Presumptive parent of a common child of a Missing Person

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INDEX Forensic STR

Convicted Offender STR

Unidentified Human Remains

Missing Person

Biological Relatives of Missing Persons

Forensic STR X X X X

Convicted Offender STR X X X

NDIS Searches

Unidentified Human Remains

X X X X X

Missing Person X X X

Biological Relatives of Missing Persons

X

Next Generation CODIS(NGCODIS)

Each NGCODIS product: - Will be fielded under “CODIS”

CODIS 6.0 (MP/DVI software): +Mito 1.4 replacementCODIS 7.X: CODIS 5.7.X replacement

- Is governed by FBI OCIO IT policies and procedures:FBI Approval of Final Design (Gate 3)FBI Approval of Final Design (Gate 3)Government Acceptance TestingFBI Approval to Test Gate (Gate 4/5)Scenario-based User Acceptance Testing Certification and AccreditationFBI Deployment Gate Review (Gate 6)

Deployment only occurs after FBI approval at Gate 6

Summary of NGCODIS Requirements CODIS 6.0 (MP/DVI)- Operate as a National Missing Persons System- Missing Persons (MP) Pedigree trees and use of metadata- Representative and Foundational profiles- Deployable to support Mass Disasters - Computer-Based Training

CODIS 7.X (5.7.X replacement)( p )- Re-architect and redevelop CODIS 5.7.X

Eliminate technology obsolescence issuesComply with FBI Enterprise ArchitectureAbility to efficiently process 50M DNA profiles Increase search algorithm performance: 50M vs. 50M (8 hrs)

- Ability to integrate new forensic DNA technologies with minor software changes

- Automated Hit Counting- Enhanced Security Features- Computer-Based Training

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CODIS 6.0 (MP/DVI) Development Status

FBI Government Acceptance Testing: CompleteUser Acceptance Testing complete: Complete- 6 sites executed user-developed operational scenarios

covering both fictional MP cases and fictional disasters

Deployment: Gate 6- FBI “Approval to Deploy within CONUS” expected Sept 08

Samples for NGCODIS

Circles are femalesSquares are males

A minimum of two Biological RelativesA minimum of two Biological Relatives

Samples for NGCODIS

Circles are femalesSquares are males

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Date of Birth of the Missing Person: Year ___ Month __ Day __

Date of last known contact with the Missing Person Year ___ Month ___ Day ___(If not you, by whom)

Last known location of the Missing Person: State ________ City or County ____________

Approximate Height of the Missing Person: _________ Feet ________ Inches

METADATA for Missing Persons

Are Dental records available: Yes __ No __ Contact Information: ______________Name and Phone Number

Population Group: American Indian/Alaskan Native Asian Black Hispanic White Hawaiian/Pacific Is.(Circle group)Other (explain)

Please provide any additional information about ancestry that could assist in identification.

List Physical Anomalies (healed broken bones) and Procedures (e.g. hip replacement) / Scars and Marks / Tattoos:

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Age Range _____ to _____ years old

Date of Recovery _____Month ___ Day ___Year

Geographic Location of Recovery _____________ City or County _______ State

GPS coordinates (if available) or approximate direction and location from a U.S. Post Office:(Include location of Post Office used in reference)

Sex of individual ___Male ___ Female ___Undetermined

P l i G

METADATA for Unidentified Human Remains

Population Group: American Indian/Alaskan Native Asian Black Hispanic White Hawaiian/Pacific Island

Other (explain)

Please provide any additional information about ancestry that could assist in identification.

Are Dental records available: Yes _______ No _______

Dental Record Contact Information: ______________________________Name and Phone Number

Height (height range) ______________________________

Scars/Marks/Tattoos

Physical Anomalies (including healed broken bones):

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DNA Analysis Unit II(Mitochondrial DNA)

DNA Analysis Unit I(Nuclear DNA)

CODIS Unit

Desiree Michelle Lekanoff

September 4, 2001Desiree Michelle Lekanoff (DOB 4/27/79) was reported missing by her boyfriend. Michelle was last seen by a friend on November 24, 2001.

May 7, 2003Investigator Hughes of the Alaska State Troopers turned over buccal samples from Antonia Lekanoff, mother of Desiree Michelle p ,Lekanoff to the FBI Field Office in Anchorage. The swabs were forwarded to the FBI’s National Missing Person DNA Database (NMPDD) Program.

June 18, 2003A decomposed, partially dismembered torso, with attached portions of upper and lower extremities was discovered on the mud flats beach near Buluga Point and McHugh Creek on the Seward Highway.

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Alaska Departmentof Public Safety

FBI Laboratory

July 10, 2003An STR (nuclear DNA) profile from the unidentified remains was developed by the State of Alaska Department of Public Safety Laboratory in Anchorage. The STR profile was entered into NDIS.

August 12, 2003Examiner Deborah Polanskey wrote a report describing theMitochondrial DNA profile determined from Antonina Lekanoff’s buccal sample The mtDNA profile was entered into the mtDNAbuccal sample. The mtDNA profile was entered into the mtDNA database. The extracts were sent to the FBI’s DNA Analysis Unit I (nuclear DNA).

May 14, 2004The STR profile from Antonina Lekanoff’s buccal sample was uploaded into NDIS. Prior to this upload, there had been six moderate stringency hits at NDIS between the unidentified human remains and convicted offender STR profiles. All of the STR hits to the convicted offender profiles were ruled out by gender and/or DNA type.

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Alaska Departmentof Public Safety

STR Locus Unidentified Remains

D3S1358 15, 16CSF1PO 10, 12D13S317 11, 11D16S539 9, 12D5S818 11, 12D8S1179 13, 14FGA 26, 26TH01 7, 7TPOX 8, 9VWA 16, 17Amelogenin X, X

Biological Mother

15, 1610, 1210, 119, 911, 1114, 1519, 267, 79, 1117, 17X, X

FBI Laboratory

Mitochondrial DNA Type

UnidentifiedRemains

16111 T16223 T

HV1 16265 G16290 T16319 A16362 C

73 G146 C

HV2 153 G235 G263 G315.1 C

BiologicalMother

16111 T16223 T16265 G16290 T16319 A16362 C

73 G146 C153 G235 G263 G315.1 C

May 28, 2004NDIS reported a moderate stringency hit between the STR profile from Antonina Lekanoff and the unidentified human remains.

June 1, 2004Chris Beheim from the State of Alaska Department of Public Safety Laboratory in Anchorage phones J.E.B. Stewart of the FBI Laboratory by phone and fax. After reviewing the information, Dr. Stewart requested that State of Alaska Department of Public Safety Laboratory send a tissue sample from the unidentified human remains.

June 22, 2004Deborah Polanskey of the FBI’s NMPDD program reported a mtDNA match between the unidentified human remains and the buccal sample. The mtDNA results were sent to the FBI Field Office in Anchorage and to the Alaska DPS Laboratory.

June 2004Using the DNA results (STR and mtDNA), the Medical Examiner’s Office from the State of Alaska Department of Health and Social Services made the determination that the unidentified human remains were those of Desiree Michelle Lekanoff.

Antonina Lekanoff was informed that the remains found on June 18 2003 were those of her daughterfound on June 18, 2003 were those of her daughter.

Desiree Michelle Lekanoff

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OMI Albuquerque

Contact Info

J. E. B. Stewart, Ph.D. [email protected] Carey 703-632-8383 [email protected]

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