POSTER PRESENTATION Open Access Co-infection of Mandrills with SIVmnd and STLV-1: implications for clinical outcome Sandrine Souquière 1 , Mirdad Kazanji 1,2* From 16th International Conference on Human Retroviruses: HTLV and Related Viruses Montreal, Canada. 26-30 June 2013 We showed previously that Mandrills infected with STLV have a variable proviral load and evidence of CTL-specific responses. STLV-infected mandrills show an activated immune system with no clear evidence of clinical manifestation or associated illness. Mandrills are also naturally infected with SIVmnd, with no T cell activation or CD4 + T cell depletion, despite a high viral load. In humans, dual infection with HIV and HTLV-1 may worsen clinical related outcomes. Our aim was to evaluate the effect of coinfection with SIVmnd and STLV-1 on viral burden, immunological changes and clinical outcome in Mandrills. We showed that SIV viral load was slightly higher in SIV-infected mandrills than in SIV+/STLV+ animals. In contrast, STLV-1 proviral load was higher in co-infected monkey than mono-infected group. Furthermore, the dually infected mandrills have statistically lower CD4+ T cells (p < 0.01). Coinfection was also associated with a decrease in the proportion of naive CD8+ T-cells (p < 0.001) and an increase in that of central memory cells (p < 0.05). A lower percentage of Ki67 was found in CD4 + and CD8 + T cells from SIV- infected animals than in the other groups. However co- infected monkeys have had the higher percentages of activated CD4 + and CD8 + T(p < 0.01). In the coinfected group, we identified two mandrills with high immune activation and clonal integration of STLV provirus. These animals presented pathological manifestations (infective dermatitis and generalized scabies) rarely encountered in nonhuman primates. These results high- light the importance of multiple infections in nonhuman primates. Authors’ details 1 Laboratoire de Rétrovirologie, Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon. 2 Institut Pasteur de Bangui, Bangui, Central African Republic. Published: 7 January 2014 doi:10.1186/1742-4690-11-S1-P41 Cite this article as: Souquière and Kazanji: Co-infection of Mandrills with SIVmnd and STLV-1: implications for clinical outcome. Retrovirology 2014 11(Suppl 1):P41. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit 1 Laboratoire de Rétrovirologie, Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon Full list of author information is available at the end of the article Souquière and Kazanji Retrovirology 2014, 11(Suppl 1):P41 http://www.retrovirology.com/content/11/S1/P41 © 2014 Souquière and Kazanji; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Co-infection of Mandrills with SIVmnd and STLV-1: implications for clinical outcome

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Page 1: Co-infection of Mandrills with SIVmnd and STLV-1: implications for clinical outcome

POSTER PRESENTATION Open Access

Co-infection of Mandrills with SIVmnd andSTLV-1: implications for clinical outcomeSandrine Souquière1, Mirdad Kazanji1,2*

From 16th International Conference on Human Retroviruses: HTLV and Related VirusesMontreal, Canada. 26-30 June 2013

We showed previously that Mandrills infected withSTLV have a variable proviral load and evidence ofCTL-specific responses. STLV-infected mandrills showan activated immune system with no clear evidence ofclinical manifestation or associated illness. Mandrills arealso naturally infected with SIVmnd, with no T cell acti-vation or CD4+ T cell depletion, despite a high viralload. In humans, dual infection with HIV and HTLV-1may worsen clinical related outcomes. Our aim was toevaluate the effect of coinfection with SIVmnd andSTLV-1 on viral burden, immunological changes andclinical outcome in Mandrills. We showed that SIV viralload was slightly higher in SIV-infected mandrills than inSIV+/STLV+ animals. In contrast, STLV-1 proviral loadwas higher in co-infected monkey than mono-infectedgroup. Furthermore, the dually infected mandrills havestatistically lower CD4+ T cells (p < 0.01). Coinfectionwas also associated with a decrease in the proportion ofnaive CD8+ T-cells (p < 0.001) and an increase in that ofcentral memory cells (p < 0.05). A lower percentage ofKi67 was found in CD4+ and CD8+ T cells from SIV-infected animals than in the other groups. However co-infected monkeys have had the higher percentages ofactivated CD4+ and CD8+ T (p < 0.01). In the coinfectedgroup, we identified two mandrills with high immuneactivation and clonal integration of STLV provirus.These animals presented pathological manifestations(infective dermatitis and generalized scabies) rarelyencountered in nonhuman primates. These results high-light the importance of multiple infections in nonhumanprimates.

Authors’ details1Laboratoire de Rétrovirologie, Centre International de Recherches Médicalesde Franceville (CIRMF), Franceville, Gabon. 2Institut Pasteur de Bangui,Bangui, Central African Republic.

Published: 7 January 2014

doi:10.1186/1742-4690-11-S1-P41Cite this article as: Souquière and Kazanji: Co-infection of Mandrills withSIVmnd and STLV-1: implications for clinical outcome. Retrovirology 201411(Suppl 1):P41.

Submit your next manuscript to BioMed Centraland take full advantage of:

• Convenient online submission

• Thorough peer review

• No space constraints or color figure charges

• Immediate publication on acceptance

• Inclusion in PubMed, CAS, Scopus and Google Scholar

• Research which is freely available for redistribution

Submit your manuscript at www.biomedcentral.com/submit

1Laboratoire de Rétrovirologie, Centre International de Recherches Médicalesde Franceville (CIRMF), Franceville, GabonFull list of author information is available at the end of the article

Souquière and Kazanji Retrovirology 2014, 11(Suppl 1):P41http://www.retrovirology.com/content/11/S1/P41

© 2014 Souquière and Kazanji; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

http://creativecommons.org/licenses/by/2.0

http://creativecommons.org/publicdomain/zero/1.0/