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Supplemental Material

Title

Inferring phylogenetic patterns of land snails of the genus Albinaria in the island of Dia (Crete,

Greece)

Authors

Aggeliki Dimopoulou1,2, Aglaia Antoniou3, Moisis Mylonas1,2, Katerina Vardinoyiannis1, Nikos

Poulakakis1,2

Table S1. Specimens used in this study. Sample codes, species names and number of specimen in this species, collection/museum

numbers (Field Code in NHMC), sampling localities, accession numbers, and references (NHMC: Natural History Museum of Crete,

p.s.: present study).

Map Code Species Field Code

(NHMC) Exact locality CoordinatesLONDD/LATDD

Accession No (GenBank)Reference

16S rRNA ITS1

1 A. retusa 01 11966 Dia island 25.2163/35.4541 KX893125 KX893187 p.s.




5 A. torticollis 01 12421 Dia island 25.2342/35.435 KX893129 KX893191 p.s.






11 A. jaeckeli 01 11966 Dia island 25.2163/35.4541 KX893135 KX893197 p.s.







18 A. teres 01 11969 Dia island 25.2033/35.4417 KX893142 KX893204 p.s.












30 A. manselli 01 8086 Kavalloi islet - northeast (east Crete) 26.2358/35.0305 KX893154 KX893216 p.s.

31 A. manselli 02 8087 Kavalloi islet - southwest (east Crete) 26.2285/35.0273 KX893155 KX893217 p.s.

32 A. teres 13 9299 Cha Gorge (east Crete) 25.8372/35.0845 KX893156 KX893218 p.s.

33 A. teres 14 14043 Kalo Chorio (east Crete) 25.5911/35.3055 KX893157 KX893219 p.s.

34 A. teres 15 2241 Moni Toplou (east Crete) 26.2427/35.2441 KX893158 KX893220 p.s.



37 A. teres 18 533 Koufonisi islet (east Crete) 26.1403/34.9351 KX893161 KX893223 p.s.

38 A. teres 19 14036 Ierapetra to Siteia (east Crete) 26.0682/35.089 KX893162 Failed p.s.

39 A. teres 20 14035 Kavousi Gorge (east Crete) 25.8614/35.1224 KX893163 KX893224 p.s.

40 A. teres 21 14038 Ampelos (east Crete) 26.2325/35.0403 KX893164 KX893225 p.s.

41 A. sturanyi 14039 Thrypti Mt, Afentis peak (east Crete) 25.8857/35.088 KX893165 KX893226 p.s.

42 A. hippolyti 13278 Almyros river (central Crete) 25.0463/35.3333 KX893166 Failed p.s.

43 A. ulrikae 13279 Paliokastro castle (central Crete) 25.0395/35.3655 KX893167 KX893227 p.s.

44 A. ulrikae 13279 Paliokastro castle (central Crete) 25.0395/35.3655 KX893168 KX893228 p.s.

45 A. violacea 13280 Fodele-Sises (central Crete) 24.8941/35.3925 KX893169 KX893229 p.s.

46 A. cretensis 13281 Kynigiana south (central Crete) 24.6843/35.3194 KX893170 KX893230 p.s.

47 A. ariadne 13284 Perama to Dafnes (central Crete) 24.754/35.3572 KX893171 KX893231 p.s.

48 A. hippolyti 13285 Kampos Apladianon (central Crete) 24.8094/35.3497 KX893172 KX893232 p.s.

49 A. corrugata 14011 Giouchtas Mt. (central Crete) 25.1453/35.2174 KX893173 KX893233 p.s.

50 A. wiesei 14012 Agios Georgios Selinari (east Crete) 25.5423/35.2847 KX893174 KX893234 p.s.

51 A. praeclara 14035 Kavousi Gorge (east Crete) 25.8614/35.1224 KX893175 KX893235 p.s.

52 A. praeclara 14035 Kavousi Gorge (east Crete) 25.8614/35.1224 KX893176 KX893236 p.s.

53 A. arthuriana 14040 Latsida-Kounali (east Crete) 25.5851/35.2817 KX893177 KX893237 p.s.

54 A. moreletiana 1 14018 Plaka – Vrouchas (east Crete) 25.7325/35.3104 KX893178 KX893238 p.s.

55 A. corrugata 11950 Dikti Mt. (east Crete) 25.4764/35.1232 KX893179 KX893239 p.s.

56 A. tenuicostata 14026 Gramvousa (west Crete) 23.5961/35.5843 KX893180 KX893240 p.s.

57 A. tenuicostata 14027 Falassarna (west Crete) 23.5901/35.4802 KX893181 KX893241 p.s.

58 A. sphakiota 13093 Imbros gorge (west Crete) 24.1666/35.2142 KX893182 KX893242 p.s.

59 A. spratti 13287 Drosia (central Crete) 24.8707/35.3431 KX893183 KX893243 p.s.

60 A. eburnea 10126 Agia Roumeli (west Crete) 23.9639/35.2321 KX893184 KX893244 p.s.

61 A. discolor 1178 Kithira island 23.0186/36.1562 KX893185 KX893245 p.s.

62 A. turrita 4466 Sifnos island 24.7248/36.9556 KX893186 KX893246 p.s.

63 A. moreletiana 2 --- Plaka AF031686 n/a Douris et al., 1998a

64 A. retusa --- Dia island AF031687 n/a Douris et al., 1998a

65 A. torticollis --- Dia island AF031688 n/a Douris et al., 1998a

66 A. torticollis x A. jaeckeli 01 --- Dia island AF031689 n/a Douris et al., 1998a

67 A. jaeckeli --- Dia island AF031690 n/a Douris et al., 1998a

68 A. torticollis --- Paximadi islet (east of Dia island) AF031691 n/a Douris et al., 1998a

69 A. teres --- Sitia (east Crete) AF031692 n/a Douris et al., 1998a

70 A. spratti --- Stroumboulas (central Crete) AF031674 AF136016 Douris et al., 1998a; Schilthuizen et al., 2004

71 A. corrugata --- Agia Fotini (east Crete) AF031680 AF136015 Douris et al., 1998a; Schilthuizen et al., 2004

72 A. ulrikae --- Ormos Alykis (central Crete) AF031676 AF136011 Douris et al., 1998a; Schilthuizen et al., 2004

73 A. cretensis --- Kynigiana (west Crete) AF031671 AF136019 Douris et al., 1998a; Schilthuizen et al., 2004

74 A. discolor --- Monemvasia (Peloponnesos) AF012079 AF254589 Douris et al., 1998b; van Moorsel et al., 2000

75 A. discolor --- Velopoula islet DQ665354 U19820 Douris et al., 2007; Schilthuizen et al., 2004

76 A. caerulea --- Vravrona (Attica) DQ665323 AY728368 Douris et al., 2007; Schilthuizen et al., 2004

77 A. caerulea --- Folegandros island DQ665324 AF136012 Douris et al., 2007; van Moorsel et al., 2000

78 A. voithii --- Taygetos (Peloponnesos) KR375677 KR375902 Kornilios et al., 2015

79 A. voithii --- Platoma (Peloponnesos) KR375691 KR375913 Kornilios et al., 2015

80 A. maculosa --- Kalamata (Peloponnesos) KR375750 KR375962 Kornilios et al., 2015

81 Isabellaria saxicola --- Ymitos (Attica) AF012080 AF136020 Douris et al., 1998b; Schilthuizen et al., 2004

Figure S1. Bayesian Inference (BI) tree reconstructed from the concatenated DNA dataset, excluding the specimens with missing data. Numbers on branches indicate posterior probabilities. The arithmetic mean -lnL= -7763.21. The MCMC convergence diagnostics revealed that a) the average standard deviation of split frequencies was 0.007143, b) the plot of the generation versus the log probability of the data (the log likelihood values) produced a "white noise" graph, c) the average Potential Scale Reduction Factor (PSRF) is 1.00 for all parameters (maximum PSRF is 1.001), and d) the minimum value of minimum Estimated Sample Sizes (ESS) was 1587.17 (ESS value below 100 may indicate that the parameter is undersampled), indicating convergence and stationarity.

Figure S2. The likelihood plot of the Albinaria’s delimitation. Upon convergence, the chain should stay at high likelihood locations most of the time and sometimes explores low likelihood locations (Zhang et al., 2013).

Figure S3. Bayesian Inference (BI) tree reconstructed from the concatenated DNA dataset after Gblocks filtering. Numbers on branches indicate posterior probabilities and bootstrap supports (BI/ML).

Figure S4. Maximum Likelihood tree reconstructed from the 16S rRNA. Numbers on branches indicate bootstrap values (ML) and posterior probabilities (BI). To test alternative phylogenetic hypothesis and relationships, we compared the best resulting ML topology to topology constrained to fit alternative hypothesis using the approximately unbiased test (AU), Shimodaira–Hasegawa (SH) test, Kishino – Hasegawa test and the weighted SH (wSH) and KH (wKH) in the program CONSEL (Shimodaira & Hasegawa, 2001). Constraint topologies were estimated in RAxML with the −g constraint option using the best scoring tree for topology tests estimated from 200 ML searches starting from random tree topologies. Results of the AU, KH, SH, and wKH and wSH tests didn’t reject the alternative hypothesis (p=0.218), which supported that A. retusa is more closely related to the other island endemics (A. jaeckeli, A. torticollis)

Figure S5. Maximum Likelihood tree reconstructed from the ITS1. Numbers on branches indicate bootstrap values (ML) and posterior probabilities (BI). To test alternative phylogenetic hypothesis and relationships, we compared the best resulting ML topology to topology constrained to fit alternative hypothesis using the approximately unbiased test (AU), Shimodaira–Hasegawa (SH) test, Kishino – Hasegawa test and the weighted SH (wSH) and KH (wKH) in the program CONSEL (Shimodaira & Hasegawa, 2001). Constraint topologies were estimated in RAxML with the −g constraint option using the best scoring tree for topology tests estimated from 200 ML searches starting from random tree topologies. Results of the AU, KH, SH, and wKH and wSH tests rejected the alternative hypothesis (p=0.005) formulated by Douris et al. (1998a), which supported the monophyly of A. jaeckeli and A. torticollis species group, and presented A. retusa as more closely related to other taxa such as A. moreletiana that to the rest of the island endemics.

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Cloud Object Storage | Store & Retrieve Data …€¦ · Web view25.2342/35.435 KX893132 KX893194 p.s. 9 A. torticollis 05 12422 Dia island 25.2408/35.4493 KX893133 KX893195 p.s