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8/13/2019 Clinical Practice Week
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CLINICAL PRACTICE WEEK
BASIC LABORATORY TECHNOLOGY
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8/13/2019 Clinical Practice Week
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Basic Lab Tech/DMLT 13 th Batch
Page 2 of 5 Department of Biomedical Sciences/FHS/MNU
CLINICAL PRACTICE WEEK
BASIC LABORATORY TECHNOLOGY
DAY 1:
Task: 1: Glass pipetting (15 minutes)
1. Measure 10ml of the solution C by using the graduated pipette
provided2. Measure 01ml of the solution D using the graduated
pipette provided
Task 2: Identification of glass ware (2minutes x7)
ITEM USE
Task 3: Approximate reagent preparation (15 minutes)
1. Prepare 50ml of 2.5% Sodium chloride less accurately
Task 4: Harvard Trip Balance (7.5 x 2 minutes)
Measure the mass of the following using the Harvard Trip Pan
Balance
A:
B:
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Basic Lab Tech/DMLT 13 th Batch
Page 3 of 5 Department of Biomedical Sciences/FHS/MNU
DAY 2:
Task 1: Serial dilution/Microtitre dilution (15 minutes)
In immunology, serial dilutions are often made of serum samples.
How could you a final
dilution of 1 to 320 be made? The sample volume is 20l. In a
micro titer plate make thestated serial doubling dilutions from the
provided Solution F, as given in the table
Well1 2 3 4 5
A1/10 1/20 1/40 1/160 1/320
Complete the following
a. Volume of sample should be taken in to the first well 20
l
b. Volume of diluent should be taken in to the first well
____________
c. Volume of diluent should be taken in to the 2 nd and the
following wells ________
d. Volume of diluted solution to be transferred from the first
well to the second
well___________
e. Dilution of the solution in the 8 th well is
______________
Procedure
1. Label the micro titer plate2. Set the correct volume in the
micropipette3. Pipette _______ l of diluent into the first well4.
Pipette _______ l of sample into the first well5. Pipette _______ l
of diluent into the wells from 2 to 86. Mix the content of first
well and transfer ____ l of solution from first well to the
second well and repeat the procedure serially with the six
remaining tubes7. Discard _______ l from the last tube.
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8/13/2019 Clinical Practice Week
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Basic Lab Tech/DMLT 13 th Batch
Page 4 of 5 Department of Biomedical Sciences/FHS/MNU
Task 2: Microscope (15 minutes)
1. Focus the given urinary sediment at 40x and show it
Task 3: Colorimeter (15 minutes)
1. Measure the absorbance of the following solution. Use
de-ionized water as thereagent blankA:B:
Task 4: pH meter (15 minutes)
1. Measure the pH of the solutions provided and record your
value
H:
J:
Task 5: Accurate reagent preparation (15 minutes)
1. Prepare 50ml of 3% Sodium Chloride accurately
Task 6: (5minutes x3)
1. Identify the labeled parts of the centrifuge and state their
function
A:
B:
C:
2. Identify the labeled parts of the autoclave
A:
B:
C
3. Read the volumes of the following
A: C:
B: D:
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8/13/2019 Clinical Practice Week
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Basic Lab Tech/DMLT 13 th Batch
Page 5 of 5 Department of Biomedical Sciences/FHS/MNU
DAY 4: Autoclave
1. Set up the provided items for a decontamination cycle2. Run a
decontamination cycle in the manual autoclave( the pressure
cooker)
