Chemistry Fermentation

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    ENDRIYA VIDAYALAYA

    AURANGABAD

    Investigatory Project

    TOPIC ! To Co"#are t$e Rate o% &er"entation o% Given'a"#(e o% )$eat %(o*r+ Gra" %(o*r+ Rice &(o*r , Potato

    '*-"itte. By ! '$*-$a" 'rivastava Ro(( No/ 01021

    C(ass ! 3II 'cience

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    CERTI&ICATE

    This is to certify that SHUBHAM

    SRIVASTAVA, a student of class XII-A has

    successfully completed the research on the

    below mentioned project under the guidance

    of MR. RAKESH KUMAR( Subject Teacher )

    during the year 20151! in partial ful"llment of#$%&'ST practical e*amination conducted

    by +'SS#%, ew -elhi.

    Signature of

    Signature of

    %*ternal e*aminer

    #hemistryTeacher

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    ACKNO)LEDGE4ENT

    I 5o*(. (i6e to ac6no5(e.ge 5it$ #(eas*re t$at o*r C$e"istry

    teac$er 74r/Ra6es$ K*"ar8 t$at 5it$ $is *se%*( , #ro#er g*i.ance/

    )e are a-(e to co"#(ete t$is #rojecte. s*ccess%*((y/

    Na"e ! '$*-$a" 'rivastava

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    DECLARATIONI .o $ere-y .ec(are t$at t$is #roject 5or6 $as -een origina((y carrie.

    *n.er t$e g*i.ance an. s*#ervision o%

    4r/ Ra6es$ K*"ar+ Ken.riya Vi.aya(aya+ A*ranga-a.

    '$*-$a" 'rivastava

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    OB9ECTIVE

    T$e #*r#ose o% t$e e:#eri"ent is! to co"#are t$e

    rate o% %er"entation o% t$e given sa"#(es o%

    5$eat %(o*r+ gra" %(o*r+ rice %(o*r , #otatoes/

    I -eco"e intereste. in t$is I.ea 5$en I sa5 so"e e:#eri"ents on

    %er"entation an. 5ante. to %in. o*t so"e scienti%ic %acts a-o*t

    %er"entation/ T$e #ri"ary -ene%it o% %er"entation is t$e conversion

    o% s*gars an. ot$er car-o$y.rates+ eg+/ Converting j*ice into 5ine+

    grains into -eer+ car-o$y.rates into car-on .io:i.e to (eaven -rea.

    an. s*gars in vegeta-(es into #reservative organic aci.s/

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    INTRODUCTION

    &ER4ENTATION ty#ica((y is t$e conversion o% car-o$y.rates to

    a(co$o( an. car-on .io:i.e or organic aci.s *sing yeasts+

    -acteria+ or a co"-ination t$ere o%+ *n.er anaero-ic

    con.itions/ A "ore restricte. .e%inition o% %er"entation is t$e

    c$e"ica( conversion o% s*gars into et$ano(/ T$e science o%

    %er"entation is 6no5n as ;y"o(ogy/ &er"entation *s*a((y

    i"#(ies t$at t$e action o% "icroorganis"s is .esira-(e+ an.

    t$e #rocess is *se. to #ro.*ce a(co$o(ic -everages s*c$ as

    5ine+ -eer+ an. ci.er/ &er"entation is a(so e"#(oye. in

    #reservation tec$ni

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    i"#(ies t$at t$e action o% "icroorganis"s is .esira-(e+ an.

    t$e #rocess is *se. to #ro.*ce a(co$o(ic -everages s*c$ as

    5ine + -eer+ an. ci.er/ &er"entation is a(so e"#(oye. in

    #reservation tec$ni

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    o% (iving yeast ce((s/ )$i(e st*.ying t$is#rocess in 0H+

    E.*ar. B*c$ner o% ?*"-o(.t University o% Ber(in+ Ger"any+

    %o*n. t$at s*gar 5as %er"ente. even 5$en t$ere 5ere no

    (iving yeast ce((s in t$e "i:t*re + -y a yeast secretion t$at $e

    ter"e. ;y"ase/ In 0H $ereceive. t$e No-e( Pri;e in

    C$e"istry %or $is researc$ an. .iscovery o% ce((!%ree

    %er"entation/FOne year #rior+ in 0+ et$ano( %er"entation

    st*.ies (e. to t$e ear(y .iscovery o% NADJ/

    Uses

    &oo. %er"entation $as -een sai. to serve %ive "ain #*r#oses

    Enric$"ent o% t$e .iet t$ro*g$ .eve(o#"ent o% a .iversity o%

    %(avo*rs+ aro"as+ an. te:t*res in %oo. s*-strates/

    Preservation o% s*-stantia( a"o*nts o% %oo. t$ro*g$ (actic aci.+

    a(co$o(+ acetic aci. an. a(6a(ine %er"entations

    Bio(ogica( enric$"ent o% %oo. s*-strates 5it$ #rotein+ essentia(

    a"ino aci.s+ essentia( %atty aci.s+ an. vita"ins

    E(i"ination o% ant n*trients/

    A .ecrease in coo6ing ti"es an. %*e( re

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    a((o5ing ani"a( #ro.*cts s*c$ as 5$o(e %is$+ %is$ $ea.s+ 5a(r*s+ sea

    (ion an. 5$a(e %(i##ers+ -eaver tai(s+ sea( oi(+ -ir.s+ etc/+ to %er"ent %or

    an e:ten.e. #erio. o% ti"e -e%ore -eing cons*"e./ T$e ris6 is

    e:acer-ate. 5$en a #(astic container is *se. %or t$is #*r#oseinstea. o% t$e o(.!%as$ione. "et$o.+ grass!(ine. $o(e+ as t$e

    -ot*(in*" -acteria t$rive in t$e anaero-ic con.itions create. -y t$e

    air!tig$t enc(os*re in #(astic/

    'a%ety o% &er"ente. &oo.s!

    &er"ente. %oo.s genera((y $ave a very goo. sa%ety recor. even int$e .eve(o#ing 5or(. 5$ere t$e %oo.s are "an*%act*re. -y #eo#(e5it$o*t training in "icro-io(ogy or c$e"istry in *n$ygienic+

    conta"inate. environ"ents/ T$ey are cons*"e. -y $*n.re.s o%

    "i((ions o% #eo#(e every .ay in -ot$ t$e .eve(o#e. an. t$e

    .eve(o#ing 5or(./ An. t$ey $ave an e:ce((ent sa%ety recor./)$at is

    t$ere a-o*t %er"ente. %oo.s t$at contri-*tes to sa%ety )$i(e

    %er"ente. %oo.s are t$e"se(ves genera((y sa%e+ it s$o*(. -e note.

    t$at %er"ente. %oo.s -y t$e"se(ves .o not so(ve t$e #ro-(e"s o%

    conta"inate. .rin6ing 5ater+ environ"ents $eavi(y conta"inate.

    5it$ $*"an 5aste+ i"#ro#er #ersona( $ygiene in %oo. $an.(ers+ %(ies

    carrying .isease organis"s+ *n%er"ente. %oo.s carrying %oo.

    #oisoning or $*"an #at$ogens an. *n%er"ente. %oo.s+ even 5$en

    coo6e. i% $an.(e. or store. i"#ro#er(y/ A(so i"#ro#er(y %er"ente.

    %oo.s can -e *nsa%e/ ?o5ever+ a##(ication o% t$e #rinci#(es t$at (ea.

    to t$e sa%ety o% %er"ente. %oo.s co*(. (ea. to an i"#rove"ent in t$e

    overa((

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    T?EORY !

    )$eat %(o*r+ gra" %(o*r+ rice %(o*r an. #otatoescontain starc$ as t$e "ajor constit*ent/ 'tarc$ #resent in t$ese %oo.

    "ateria(s is %irst -ro*g$t into so(*tion/ In t$e #resence o% en;y"e.iastase+ starc$ *n.ergoes %er"entation to give "a(tose/ 'tarc$

    gives -(*e!vio(et co(o*r 5it$ io.ine 5$ereas #ro.*ct o% %er"entation

    starc$ .oes not give any c$aracteristic co(o*r/ )$en t$e

    %er"entation is co"#(ete t$e reaction "i:t*re sto#s giving -(*e!

    vio(et co(o*r 5it$ io.ine so(*tion/ By co"#aring t$e ti"e re

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    en;y"e .iastase is o-taine. -y ger"ination o% "oist -ar(ey see.s in

    .ar6 at 0 .egree Ce(si*s/ )$en t$e ger"ination is co"#(ete t$e

    te"#erat*re is raise. to .egree Ce(si*s to sto# %*rt$er gro5t$/

    T$e see.s are cr*s$e. into 5ater an. %i(tere./ T$e %i(trate containsen;y"e .iastase an. is ca((e. "a(t e:tract/

    4ATERIAL' REMUIRED !

    Conica( %(as6

    Test t*-e

    &*nne(

    &i(ter #a#er

    )ater -at$

    0 Io.ine so(*tion

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    Yeast

    )$eat %(o*r

    Gra" %(o*r

    Rice %(o*r

    Potato

    A

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    Kee# t$is %(as6 in a 5ater -at$ "aintaine. at a te"#erat*re o% !

    .egree ce(si*s/ A.. 1 "( o% "a(t e:tract/

    A%ter 1 "in*tes ta6e 1 .ro#s o% t$e reaction "i:t*re an. a.. to

    .i(*te. io.ine so(*tion/

    Re#eat ste# a%ter every 1 "in*tes/ )$en no -(*is$ co(o*r is

    #ro.*ce. t$e %er"entation is co"#(ete/

    Recor. t$e tota( ti"e ta6en %or co"#(etion o% %er"entation/

    Re#eat t$e e:#eri"ent 5it$ gra" %(o*r e:tract+ rice %(o*r e:tract+

    #otato e:tract an. recor. t$e o-servations/

    OB'ERVATION!

    Accor.ing to e:#eri"ent

    S no. Object Fermented Time Required (hr)

    1 Wheat flour 10

    2 Gram flour 12.

    ! Rice flour 1

    " #otato 1!

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    Conc(*sion !

    Rice %(o*r ta6es "a:i"*" ti"e %or %er"entation an. 5$eat %(o*r

    ta6es t$e "ini"*" ti"e %or %er"entation/

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    BIBLIOGRAP?Y

    )i6i#e.ia!t$e %ree enc(yc(o#e.ia

    C$e"istry "an*a(

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    T$% %-

    T$+/