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© 2014, Charles River Laboratories International, Inc.
Technical Book
Charles River Immunodeficient Models Xenograft Data Catalog
Fox Chase SCID® Congenic (C.B-17 SCID) Mouse
Xenograft Data
Technical Book
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
Technical Book
Table of Contents1 Introduction
2-11 Severe Combined Immunodeficiency (SCID) Models
2-11 Fox Chase SCID® Congenic Mouse
2 Bladder - Cell Line: SW780-e203
2 Blood - Cell Line: Daudi-e207
2 Blood - Cell Line: DOHH-2-e207
3 Bone - Cell Line: Saos-2-e202
3 Brain - Cell Line: SF295-v2-e200
3 Breast - Cell Line: BT474-e221
3 Breast - Cell Line: JIMT-1-e203
3 Breast - Cell Line: SKBR3-e200
4 Head and Neck - Cell Line:
SK-GT-e203
4 Kidney - Cell Line: Caki-1-e203
4 Leukemia (Blood) - Cell Line:
K562-e205
4 Leukemia (Blood) - Cell Line:
MOLT-4-e200
5 Liver - Cell Line: Hep3B-e220
5 Liver - Cell Line: HepG2-e201
5 Lung - Cell Line: Calu3-e200
5 Lung - Cell Line: H810-e202
5 Lung - Cell Line: H929-e204
5 Lung - Cell Line: MSTO-211H-e202
6 Lymphoma (Blood) - Cell Line:
Ramos-e213
6 Lymphoma (Blood) - Cell Line:
RL-e209
6 Lymphoma (Blood) - Cell Line:
SU-DHL-6-e204
6 Melanoma - Cell Line: SKMEL-5-e203
7 Multiple Myeloma (Blood) - Cell Line:
H929-e204
7 Multiple Myeloma (Blood) - Cell Line:
OPM-2-e206
7 Multiple Myeloma (Blood) - Cell Line:
RPMI-8226-e207
8 Ovarian - Cell Line: A2780-e307
8 Ovarian - Cell Line: OVCAR3-e226
8 Ovarian - Cell Line: OVCAR5-e203
8 Ovarian - Cell Line: SKOV3-e206
9 Pancreas - Cell Line: Capan-1-e204
9 Pancreas - Cell Line: Capan-1-e205
9 Pancreas - Cell Line: KP4-e200
9 Pancreas - Cell Line: MiaPaCa-2-e276
10 Prostate - Cell Line: CWR22Rv1-e227
10 Prostate - Cell Line: LNCaP-e222
10 Skin - Cell Line: A375-e319
10 Skin - Cell Line: A2058-e269
11 Stomach - Cell Line: MKN-45-e201
11 Stomach - Cell Line: NCI-N87-e213
11 Stomach - Cell Line: SNU5-e206
11 Thyroid - Cell Line: 8505c-e203
12-14 Cell Line Testing - 360 Diagnostics
12 Rodent CLEAR
13 Human CLEAR
13 Contamination CLEAR
14 Discovery Services
14 Collection of Oncology Research Experiments (CORE)
14 Supplemental Services
Technical Book
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
Technical Book
1
IntroductionOverviewOncology is one of the leading areas of research into new therapeutics. Due to the challenges inherent in researching and developing anticancer therapeutics, it is critical that you have the right tools and resources available to you. Backed by decades of technical, scientific and veterinary experience, Charles River’s global portfolio of high-quality oncology models gives you the benefit of partnering with an industry leader offering an infrastructure capable of advancing your research now and in the future. The following information provides an overview of Charles River’s portfolio of immunodeficient and immunocompetent oncology animal models produced in North America.
Selecting the Appropriate Immunodeficient Animal ModelImmunodeficient animal models are extremely useful in a wide range of biomedical research, including infectious disease, stem cell, immunology and oncology studies. Due to the unique vulnerability that makes these models vital to research, their care and maintenance demands a high level of expertise and technological resources.
Selecting the most appropriate animal model to use is one of the more challenging steps a researcher must take when designing an oncology study. Quite often, resources and time are logged performing literature searches for what has been previously published or conducting trial and error pilot studies to assess positive tumor growth. As such, Charles River has developed the following information in an effort to assist you in expediting your model selection process.
The following table illustrates the Charles River portfolio of immunodeficient animal models. Depending on your research design and cell line, it is critical to understand the level of immunodeficiency each model possesses.
Xenograft Data CatalogThe following section contains tumor growth data collected from xenograft studies that were conducted by Charles River Discovery Services. This data represents various cell lines and histotypes implanted and grown in select Charles River immunodeficient animal models and should be used as reference data only. Volume 1 contains xenograft data* for the following models:
*Charles River will continue to expand our data catalog in upcoming volumes to include additional data on immunodeficient animal models produced by
Charles River.
• Athymic Nude Mouse
• Fox Chase SCID® Congenic Mouse (C.B-17 SCID)
• Fox Chase SCID® Beige Mouse
• SCID Hairless Outbred (SHO®) Mouse
Strain Hair T-Cell Deficient B-Cell Deficient NK Cell Deficient
Athymic Nude Mouse No Yes No No
CD-1® Nude Mouse No Yes No No
NU/NU Mouse No Yes No No
BALB/c Nude Mouse No Yes No No
NIH-III Mouse No Yes Yes Impaired
RNU Rat No Yes No No
SCID Hairless Outbred (SHO®) Mouse No Yes Yes No
SCID Hairless Congenic (SHC™) Mouse No Yes Yes No
Fox Chase SCID® Congenic Mouse Yes Yes Yes No
Fox Chase SCID® Beige Mouse Yes Yes Yes Impaired
NOD SCID Mouse Yes Yes Yes Impaired
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
2
Fox Chase SCID® Congenic Mouse (C.B-17 SCID)Strain Code: 236Nomenclature: CB17/Icr-Prkdcscid/IcrIcoCrlOrigin: SCID mice possess a genetic autosomal recessive mutation (SCID). Discovered in 1980 by Bosma in C.B-17/Icr mice at Fox Chase Cancer Center. SCID mice show a severe combined immunodeficiency affecting both B and T lymphocytes. They have normal natural killer (NK) cells, macrophages and granulocytes. To Charles River in 1991 from a Charles River France foundation colony.
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1 4 8 11 15 18 22 25 29 32 36 39 43
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Bladder|Cell Line: SW780-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
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2700
3000
1 3 7 10 14 17 21 24 28 31
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Blood|Cell Line: Daudi-e207
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
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3500
4000
4500
1 4 9 12 16 19
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Blood|Cell Line: DOHH-2-e207
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
Bladder
Blood
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Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
Technical Book
3
0
20
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240
8 12 15 19 22 26 33 37 41
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10Animal 11 Animal 12 Animal 13 Animal 14 Animal 15
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Breast|Cell Line: SKBR3-e200
Animal 11 Animal 12 Animal 13 Animal 14 Animal 15
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
0
20
40
60
80
100
120
140
160
180
200
8 11 15 18 22 25 29 32 36 39 43 50 57 60 64
Days
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5
Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Bone|Cell Line: Saos-2-e202
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
0
100
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1000
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1 4 7 11 14 18 21 25 28 32 35 39 42 46 49 53 56 60
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Breast|Cell Line: JIMT-1-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
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800
1000
1200
1 4 8 11 15 18 22 25 29 32 36 39 43 46
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Breast|Cell Line: BT474-e221
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
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2800
3200
3600
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4400
1 4 8 11 15 18
Days
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5 Animal 6 Animal 7 Animal 8 Animal 9
EndpointTu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Brain|Cell Line: SF295-v2-e200
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
Bone Brain
Breast
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
4
Leukemia (Blood)
0
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1 5 8 12 15 20 23 27 30 34 37 41 44 48 51 55
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Head and Neck|Cell Line: SK-GT-4-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1400
1 4 8 11
Animal 1 Animal 2 (ES) Animal 3 Animal 4 (ES) Animal 5 (ES)
Animal 6 Animal 7 (TP) Animal 8 (ES) Animal 9 (ES) Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Leukemia (Blood)|Cell Line: K562-e205
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
1 4 9 13 16 20 23 27 30 34 37 41 44 49
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Leukemia (Blood)|Cell Line: MOLT-4-e200
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1 4 8 11 15 18 22 25 29 32 36 39 43 46 50 53 57 60 64 67 71 74
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Kidney|Cell Line: Caki-1-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
Head and Neck Kidney
Technical Book
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
5
0
300
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1500
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2400
1 4 9 13 16 20 23 27 30 34 37 41 44 48
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Liver|Cell Line: Hep3B-e220
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
2700
1 4 7 11 14 18 21 26 29 33 36 39 42
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP)Animal 5 (TP) Animal 6 (TP) Animal 7 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lung|Cell Line: H810-e202
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7
Endpoint
0
200
400
600
800
1000
1200
1400
1 4 8 12 15 19 22 26 29 33 36 40 43 47
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP)
Animal 5 (TP) Animal 6 (TP) Animal 7 (TP) Animal 8 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lung|Cell Line: MSTO-211H-e202
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8
Endpoint
0
300
600
900
1200
1500
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2100
2400
2700
3000
3300
1 3 7 10 14 17 21 24 28 31 35
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lung|Cell Line: H929-e204
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
8 12 15 16 22 26 29 33 36 40 43 47 50 54 57 61 64 68 71 75 78 82 85 89 92 95
Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5
Animal 6 (TP) Animal (TP) Animal 8 Animal 9 Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Liver|Cell Line: HepG2-e201
Endpoint
Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
0
200
400
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800
1000
1200
1400
1 4 9 13 16 20 23 27 30 34 37 41 44 48 51 55 58 62 65 69 72 76 79 83 86 90 94
Animal 1 (TP) Animal 2 (NTRu) Animal 3 (TP) Animal 4 (TP)
Animal 5 (TP) Animal 6 (TP) Animal 7 (TP) Animal 8 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lung|Cell Line: Calu3-e200
Endpoint
Animal 1 Animal 2 (NTRu)* Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8
*NTRu - nontreatment - related death
Liver
Lung
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
6
Melanoma
0
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2800
3200
3600
4000
1 4 9 12 16
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP) Animal 6 (TP)
Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP) Animal 11(TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lymphoma (Blood)|Cell Line: Ramos-e213
Animal 11
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
2700
3000
1 4 7 10 14 17 21 24 28 31 35
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Lymphoma (Blood)|Cell Line: RL-e209
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
2700
1 3 7 10 15 17 21 24 28 31 35 38 42 45 49 52 56 59 63
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 Animal 9 (TP) Animal 10 (TP)
EndpointTu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Lymphoma (Blood)|Cell Line: SU-DHL-6-e204
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1400
1600
1 4 8 12 15 19 22 26 29 33 36 40 43 47 50 54
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Melanoma |Cell Line: SKMEL-5-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
Lymphoma (Blood)
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Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
Technical Book
7
Multiple Myeloma (Blood)
0
300
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2100
2400
2700
3000
3300
1 3 7 10 14 17 21 24 28 31 35 38
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: H929-e204
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
400
800
1200
1600
2000
2400
2800
3200
3600
1 4 8 11 15 18 22 25 29 32 36
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: OPM-2-e206
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
2700
1 4 9 13 16 20 23 27 30 34 37 41 44 48 51 55 58
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: RPMI-8226-e207
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
8
Ovarian
0
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3200
1 5 9 13 16 20 23 27 30
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Ovarian|Cell Line: A2780-e307
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1400
1 4 9 12 16 19 23 26 29 33 37 40 44 47 51 54 58 61 65 68 72 75 79 82 86 89 93 96 100 104
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 Animal 9 (TP) Animal 10 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Ovarian|Cell Line: OVCAR3-e226
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1400
1600
1800
1 4 8 11 14 18 21 25 28 32 35 39 42 49 49 53 56 60 63
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Ovarian|Cell Line: OVCAR5-e203
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
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1800
2100
2400
2700
1 5 8 12 15 19 22 26 29 33 36 40 43 47 50 54
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
Days
Tu
mo
r Vol
um
e (m
m3)
CB17SCIDTissue: Ovarian|Cell Line: SKOV3-e206
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
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Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
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9
Pancreas
0
100
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800
900
1000
1 4 9 13 16 21 24 27 30 34 37 41 44 48 51 56 59 63 66 70 72 76 79 83 86 90
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP)
Animal 5 (TP) Animal 6 (TP) Animal 7 (TP) Animal 8 (TP)
Endpoint
CB17SCIDTissue: Pancreas|Cell Line: Capan-1-e204
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8
Endpoint
0
100
200
300
400
500
600
700
800
900
1000
1 4 8 12 15 19 22 27 30 33 36 40 43 48 51 54 57 61 64 68 71 75 78 83 86 90
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17SCIDTissue: Pancreas|Cell Line: Capan-1-e205
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
400
800
1200
1600
2000
2400
2800
3200
8 13 16 21 24 28 31 35 38 42
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17SCIDTissue: Pancreas|Cell Line: KP4-e200
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
400
800
1200
1600
2000
2400
2800
3200
3600
7 11 14 18 21 25 28 32 35 39 42 46 49 54 57 60 63 67 71 74 77 81 85
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17SCIDTissue: Pancreas|Cell Line: MiaPaCa-2-e276
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1
10
0
50
100
150
200
250
300
350
400
450
500
550
1 4 7 11 14 18 21 25 28 32 39 46 53 55 59
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10Animal 11 Animal 12 Animal 13 Animal 14 Animal 15
CB17SCIDTissue: Prostate|Cell Line: LNCaP-e222
Animal 11 Animal 12 Animal 13 Animal 14 Animal 15
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
0
400
800
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1600
2000
2400
2800
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1 4 8 12 16 19 23 26 30 33 37
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
CB17 SCIDTissue: Skin|Cell Line: A375-e319
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
1 5 8 12 15 19 22 26 29 33 36 40 43 47 50 54
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (NTRu) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17SCIDTissue: Prostate|Cell Line: CWR22Rv1-e227
Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
1 4 8 11 15 18 22 25 29 32 36 39 43 46
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (NTRu) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (NTRu) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17 SCIDTissue: Skin|Cell Line: A2058-e269
Endpoint
Animal 1 (NTRu)* Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 (NTRu)*
*NTRu - nontreatment - related death
Animal 9 Animal 10
Skin
Prostate
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Stomach
Thyroid
0
300
600
900
1200
1500
1800
2100
2400
1 2 5 9 12 16 19 23 26 30 33 37 40 44 47 51 54 58 62
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5
Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
CB17 SCIDTissue: Stomach|Cell Line: MKN-45-e201
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
200
400
600
800
1000
1200
1400
1600
1800
1 4 8 12 15 19 22 26 29 33 36 40 43 47 50 54 57 61 34 68 71 75 78
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)
Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)
Endpoint
CB17 SCIDTissue: Stomach|Cell Line: NCI-N87-e213
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
0
300
600
900
1200
1500
1800
2100
2400
1 4 7 10 13 16 21 24 28 31 35 38
Tu
mo
r Vol
um
e (m
m3)
Days
Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)
Endpoint
CB17 SCIDTissue: Stomach|Cell Line: SNU5-e206
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9
Endpoint
0
100
200
300
400
500
600
700
800
900
1 6 9 13 16 20 23 27 31 34
Tu
mo
r Vol
um
e (m
m3)
Days
CB17SCIDTissue: Thyroid|Cell Line: 8505c-e203
Animal 1 (ES) Animal 2 Animal 3 Animal 4 (ES) Animal 5
Animal 6 (ES) Animal 7 Animal 8 Animal 9 (ES) Animal 10 (ES)
Endpoint
Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10
Endpoint
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Cell Line Testing – 360 DiagnosticsResearch biologics of rodent or human origin are often introduced into research animals as part of an investigative procedure. Precautionary screening should be performed to confirm that biologics are free of infectious agents and that they are originating from the appropriate host species.
Rodent CLEARResearch biologics are risk factors for vivarium biosecurity. Furthermore, a contamination may compromise the integrity of your in vivo research. Charles River offers TaqMan® PCR testing on the OpenArray® platform so you can perform precautionary screening on your research biologics to confirm that they are free of rodent infectious agents.
PCR Panels to Screen Cell Lines and Research Biologics for Rodent Infectious Agents
Agent Mouse Essential Panel Rat Essential PanelMouse/Rat
Comprehensive Panel
Murine norovirus (MNV) • •
Mouse parvoviruses* (MPV-1, MPV-2, MPV-3, MPV-4, MVM) • •
Mouse hepatitis virus (MHV) • •
Reovirus (Type 1 & 3) (REO) • • •
Lymphocytic choriomeningitis virus (LCMV) • • •
Lactate dehydrogenase-elevating virus (LDV) • • •
Mouse rotavirus (MRV/EDIM) • •
Theiler’s murine encephalomyelitis virus (TMEV [GDVII]) • • •
Mousepox (Ectromelia) (ECTRO) • •
Hantavirus hantaan (HANT) • •
Hantavirus seoul (SEO) • •
Polyoma virus (POLY) • •
K virus (K) •
Mouse adenovirus (MAV-1 & MAV-2) • •
Mouse cytomegalovirus (MCMV) •
Mouse thymic virus (MTLV) •
Pneumonia virus of mice (PVM) •
Sendai (SEND) • • •
Rat cytomegalovirus (RCMV) • •
Rat theilovirus (Theiler’s-like virus of rats [RTV]) • •
Rat parvoviruses* (RPV, KRV, RMV, H-1) • •
Rat rotavirus (IDIR) • •
Rat coronavirus (RCV, SDAV) • •
Mycoplasma (genus) (including Acholeplasma laidlawii) • • •
Mycoplasma pulmonis • • •
Positive template control • • •
Negative template control • • •
Spike inhibition control • • •
Nucleic acid recovery control (NARC) • • •
*Strain determination assays are performed on all positive results.
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Human CLEARTo help maintain the validity of your in vivo research, it is important to perform precautionary screening on your research biologics to confirm that they are free of infectious agents. Charles River provides TaqMan® PCR testing to identify human infectious agents in any of your research biologics. TaqMan® technology is ten to one hundred times more sensitive than traditional gel-based qualitative PCR, and the use of an internal probe provides incomparable specificity. This technology allows samples to be analyzed without opening reaction tubes, which prevents the release of potentially contaminating PCR products, a common downfall associated with gel-based PCR assays.
PCR Panels to Screen Cell Lines and Research Biologics for Human Infectious Agents
Contamination CLEARThe Cell Line Examination and Report (CLEAR) PCR Panel allows you to check the identity of your cell lines. It can detect inter-species contamination of less than 0.5% using TaqMan® PCR assays. Cells that can be differentiated include those originating from mouse, rat, Chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) species.
Agent Human HEP/HIV PanelHuman Essential
PanelHuman
Comprehensive Panel
Polyomavirus (John Cunningham virus) • •
Polyomavirus (BK virus) • •
Herpesvirus type 6 • •
Herpesvirus type 7 • •
Herpesvirus type 8 • •
Parvovirus B19 • •
Epstein-Barr virus • •
Hepatitis A virus • •
Hepatitis B virus • • •
Hepatitis C virus • • •
Papillomavirus type 16 • •
Papillomavirus type 18 • •
Human T-lymphotropic virus • •
Human cytomegalovirus • •
Human immunodeficiency virus type 1 • • •
Human immunodeficiency virus type 2 • • •
Adeno-associated virus • •
Human foamy virus • •
Mycoplasma (genus) (including Acholeplasma laidlawii) • • •
Lymphocytic choriomeningitis virus (LCMV) •
Hantavirus hantaan •
Hantavirus seoul •
Spike inhibition control • • •
Nucleic acid recovery control (NARC) • • •
Positive template control • • •
Negative template control • • •
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Discovery ServicesCharles River provides discovery services with extensive experience in cancer pharmacology and specialty oncology models. Our broad range of models and support services allows clients to choose the most appropriate study design and screening method to identify promising compounds and optimize lead candidates.
• In Vitro Assays
• In Vivo Pharmacology Services:
- Human tumor xenografts
- Patient-derived xenografts
- Syngeneic models
- Orthotopic models
• Patient-Derived Human Tumor Grafts
• Integrated Drug Discovery
Collection of Oncology Research Experiments (CORE)The CORE (Collection of Oncology Research Experiments) is an online library of peer-reviewed publications designed to help you find the most appropriate research model for your oncology cell lines. Search through the publications by first selecting your tissue type.Simply select the cell line you want to reference and the publications will be listed by animal model. The publications were collected from various online resources and are non-curated. Visit www.criver.com/core for publications.
Supplemental ServicesCharles River’s global infrastructure gives you access to unparalleled products and services in the field of oncology. Whether you are in basic research and discovery or are ready to move your therapeutic into the clinical development phase, we are strategically positioned to help you accelerate your cancer research and anticancer drug development efforts.
Safety Assessment• General Toxicology Studies
• Infusion Toxicology Studies
• IND-Enabling Programs
• Pathology Services
• Drug Metabolism and Pharmacokinetics
• Laboratory Sciences
Clinical Support• Clinical Bioanalysis
• Clinical Pharmacokinetics
• Clinical Immunology and Biomarker Services
• Pathology for Clinical Trials
Manufacturing Support• GMP-Compliant Biologics Testing
• Endotoxin Detection and Microbial Identification