Charles River Immunodeficient Models Xenograft Data Catalog · Research Models Immunodeficient Models enograft Data Catalog - Volume 1 2 Fox Chase SCID® Congenic Mouse (C.B-17 SCID)

© 2014, Charles River Laboratories International, Inc.

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Technical Book

Charles River Immunodeficient Models Xenograft Data Catalog

Fox Chase SCID® Congenic (C.B-17 SCID) Mouse

Xenograft Data

Technical Book

Research Models Immunodeficient Models Xenograft Data Catalog - Volume 1

Technical Book

Table of Contents1 Introduction

2-11 Severe Combined Immunodeficiency (SCID) Models

2-11 Fox Chase SCID® Congenic Mouse

2 Bladder - Cell Line: SW780-e203

2 Blood - Cell Line: Daudi-e207

2 Blood - Cell Line: DOHH-2-e207

3 Bone - Cell Line: Saos-2-e202

3 Brain - Cell Line: SF295-v2-e200

3 Breast - Cell Line: BT474-e221

3 Breast - Cell Line: JIMT-1-e203

3 Breast - Cell Line: SKBR3-e200

4 Head and Neck - Cell Line:

SK-GT-e203

4 Kidney - Cell Line: Caki-1-e203

4 Leukemia (Blood) - Cell Line:

K562-e205

4 Leukemia (Blood) - Cell Line:

MOLT-4-e200

5 Liver - Cell Line: Hep3B-e220

5 Liver - Cell Line: HepG2-e201

5 Lung - Cell Line: Calu3-e200

5 Lung - Cell Line: H810-e202

5 Lung - Cell Line: H929-e204

5 Lung - Cell Line: MSTO-211H-e202

6 Lymphoma (Blood) - Cell Line:

Ramos-e213


RL-e209


SU-DHL-6-e204

6 Melanoma - Cell Line: SKMEL-5-e203

7 Multiple Myeloma (Blood) - Cell Line:

H929-e204


OPM-2-e206


RPMI-8226-e207

8 Ovarian - Cell Line: A2780-e307

8 Ovarian - Cell Line: OVCAR3-e226

8 Ovarian - Cell Line: OVCAR5-e203

8 Ovarian - Cell Line: SKOV3-e206

9 Pancreas - Cell Line: Capan-1-e204

9 Pancreas - Cell Line: Capan-1-e205

9 Pancreas - Cell Line: KP4-e200

9 Pancreas - Cell Line: MiaPaCa-2-e276

10 Prostate - Cell Line: CWR22Rv1-e227

10 Prostate - Cell Line: LNCaP-e222

10 Skin - Cell Line: A375-e319

10 Skin - Cell Line: A2058-e269

11 Stomach - Cell Line: MKN-45-e201

11 Stomach - Cell Line: NCI-N87-e213

11 Stomach - Cell Line: SNU5-e206

11 Thyroid - Cell Line: 8505c-e203

12-14 Cell Line Testing - 360 Diagnostics

12 Rodent CLEAR

13 Human CLEAR

13 Contamination CLEAR

14 Discovery Services

14 Collection of Oncology Research Experiments (CORE)

14 Supplemental Services

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1

IntroductionOverviewOncology is one of the leading areas of research into new therapeutics. Due to the challenges inherent in researching and developing anticancer therapeutics, it is critical that you have the right tools and resources available to you. Backed by decades of technical, scientific and veterinary experience, Charles River’s global portfolio of high-quality oncology models gives you the benefit of partnering with an industry leader offering an infrastructure capable of advancing your research now and in the future. The following information provides an overview of Charles River’s portfolio of immunodeficient and immunocompetent oncology animal models produced in North America.

Selecting the Appropriate Immunodeficient Animal ModelImmunodeficient animal models are extremely useful in a wide range of biomedical research, including infectious disease, stem cell, immunology and oncology studies. Due to the unique vulnerability that makes these models vital to research, their care and maintenance demands a high level of expertise and technological resources.

Selecting the most appropriate animal model to use is one of the more challenging steps a researcher must take when designing an oncology study. Quite often, resources and time are logged performing literature searches for what has been previously published or conducting trial and error pilot studies to assess positive tumor growth. As such, Charles River has developed the following information in an effort to assist you in expediting your model selection process.

The following table illustrates the Charles River portfolio of immunodeficient animal models. Depending on your research design and cell line, it is critical to understand the level of immunodeficiency each model possesses.

Xenograft Data CatalogThe following section contains tumor growth data collected from xenograft studies that were conducted by Charles River Discovery Services. This data represents various cell lines and histotypes implanted and grown in select Charles River immunodeficient animal models and should be used as reference data only. Volume 1 contains xenograft data* for the following models:

*Charles River will continue to expand our data catalog in upcoming volumes to include additional data on immunodeficient animal models produced by

Charles River.

• Athymic Nude Mouse

• Fox Chase SCID® Congenic Mouse (C.B-17 SCID)

• Fox Chase SCID® Beige Mouse

• SCID Hairless Outbred (SHO®) Mouse

Strain Hair T-Cell Deficient B-Cell Deficient NK Cell Deficient

Athymic Nude Mouse No Yes No No

CD-1® Nude Mouse No Yes No No

NU/NU Mouse No Yes No No

BALB/c Nude Mouse No Yes No No

NIH-III Mouse No Yes Yes Impaired

RNU Rat No Yes No No

SCID Hairless Outbred (SHO®) Mouse No Yes Yes No

SCID Hairless Congenic (SHC™) Mouse No Yes Yes No

Fox Chase SCID® Congenic Mouse Yes Yes Yes No

Fox Chase SCID® Beige Mouse Yes Yes Yes Impaired

NOD SCID Mouse Yes Yes Yes Impaired


2

Fox Chase SCID® Congenic Mouse (C.B-17 SCID)Strain Code: 236Nomenclature: CB17/Icr-Prkdcscid/IcrIcoCrlOrigin: SCID mice possess a genetic autosomal recessive mutation (SCID). Discovered in 1980 by Bosma in C.B-17/Icr mice at Fox Chase Cancer Center. SCID mice show a severe combined immunodeficiency affecting both B and T lymphocytes. They have normal natural killer (NK) cells, macrophages and granulocytes. To Charles River in 1991 from a Charles River France foundation colony.

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CB17SCIDTissue: Bladder|Cell Line: SW780-e203

Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9

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CB17SCIDTissue: Blood|Cell Line: Daudi-e207

Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10

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CB17SCIDTissue: Blood|Cell Line: DOHH-2-e207


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Bladder

Blood

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Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10Animal 11 Animal 12 Animal 13 Animal 14 Animal 15

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CB17SCIDTissue: Breast|Cell Line: SKBR3-e200

Animal 11 Animal 12 Animal 13 Animal 14 Animal 15


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CB17SCIDTissue: Bone|Cell Line: Saos-2-e202


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Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)

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CB17SCIDTissue: Breast|Cell Line: JIMT-1-e203


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CB17SCIDTissue: Breast|Cell Line: BT474-e221


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CB17SCIDTissue: Brain|Cell Line: SF295-v2-e200


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Bone Brain

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Leukemia (Blood)

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CB17SCIDTissue: Head and Neck|Cell Line: SK-GT-4-e203


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Animal 1 Animal 2 (ES) Animal 3 Animal 4 (ES) Animal 5 (ES)

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CB17SCIDTissue: Leukemia (Blood)|Cell Line: K562-e205


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CB17SCIDTissue: Leukemia (Blood)|Cell Line: MOLT-4-e200


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CB17SCIDTissue: Kidney|Cell Line: Caki-1-e203


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Head and Neck Kidney

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CB17SCIDTissue: Liver|Cell Line: Hep3B-e220


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CB17SCIDTissue: Lung|Cell Line: H810-e202

Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7

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CB17SCIDTissue: Lung|Cell Line: MSTO-211H-e202

Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8

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CB17SCIDTissue: Lung|Cell Line: H929-e204


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Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5

Animal 6 (TP) Animal (TP) Animal 8 Animal 9 Animal 10 (TP)

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CB17SCIDTissue: Liver|Cell Line: HepG2-e201

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Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10

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CB17SCIDTissue: Lung|Cell Line: Calu3-e200

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Animal 1 Animal 2 (NTRu)* Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8

*NTRu - nontreatment - related death

Liver

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Melanoma

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CB17SCIDTissue: Lymphoma (Blood)|Cell Line: Ramos-e213

Animal 11


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CB17SCIDTissue: Lymphoma (Blood)|Cell Line: RL-e209


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Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 Animal 9 (TP) Animal 10 (TP)

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CB17SCIDTissue: Lymphoma (Blood)|Cell Line: SU-DHL-6-e204


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CB17SCIDTissue: Melanoma |Cell Line: SKMEL-5-e203


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Lymphoma (Blood)

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Multiple Myeloma (Blood)

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CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: H929-e204


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CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: OPM-2-e206


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CB17SCIDTissue: Multiple Myeloma (Blood)|Cell Line: RPMI-8226-e207


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Ovarian

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CB17SCIDTissue: Ovarian|Cell Line: A2780-e307


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Animal 6 (TP) Animal 7 (TP) Animal 8 Animal 9 (TP) Animal 10 (TP)

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CB17SCIDTissue: Ovarian|Cell Line: OVCAR3-e226


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Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)

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CB17SCIDTissue: Ovarian|Cell Line: OVCAR5-e203


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CB17SCIDTissue: Ovarian|Cell Line: SKOV3-e206


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Pancreas

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CB17SCIDTissue: Pancreas|Cell Line: Capan-1-e204

Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8

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CB17SCIDTissue: Pancreas|Cell Line: Capan-1-e205


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CB17SCIDTissue: Pancreas|Cell Line: KP4-e200


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Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 (TP) Animal 9 (TP) Animal 10 (TP)

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CB17SCIDTissue: Pancreas|Cell Line: MiaPaCa-2-e276


Endpoint


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Animal 1 Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10Animal 11 Animal 12 Animal 13 Animal 14 Animal 15

CB17SCIDTissue: Prostate|Cell Line: LNCaP-e222



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CB17 SCIDTissue: Skin|Cell Line: A375-e319


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Animal 1 (TP) Animal 2 (NTRu) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)


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CB17SCIDTissue: Prostate|Cell Line: CWR22Rv1-e227

Animal 1 Animal 2 (NTRu) Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 Animal 9 Animal 10

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Animal 6 (TP) Animal 7 (TP) Animal 8 (NTRu) Animal 9 (TP) Animal 10 (TP)

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CB17 SCIDTissue: Skin|Cell Line: A2058-e269

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Animal 1 (NTRu)* Animal 2 Animal 3 Animal 4 Animal 5Animal 6 Animal 7 Animal 8 (NTRu)*

*NTRu - nontreatment - related death

Animal 9 Animal 10

Skin

Prostate

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Stomach

Thyroid

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CB17 SCIDTissue: Stomach|Cell Line: MKN-45-e201


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CB17 SCIDTissue: Stomach|Cell Line: NCI-N87-e213


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Animal 1 (TP) Animal 2 (TP) Animal 3 (TP) Animal 4 (TP) Animal 5 (TP)Animal 6 (TP) Animal 7 (TP) Animal 8 (TP) Animal 9 (TP)

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CB17 SCIDTissue: Stomach|Cell Line: SNU5-e206


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CB17SCIDTissue: Thyroid|Cell Line: 8505c-e203

Animal 1 (ES) Animal 2 Animal 3 Animal 4 (ES) Animal 5

Animal 6 (ES) Animal 7 Animal 8 Animal 9 (ES) Animal 10 (ES)

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Endpoint


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Cell Line Testing – 360 DiagnosticsResearch biologics of rodent or human origin are often introduced into research animals as part of an investigative procedure. Precautionary screening should be performed to confirm that biologics are free of infectious agents and that they are originating from the appropriate host species.

Rodent CLEARResearch biologics are risk factors for vivarium biosecurity. Furthermore, a contamination may compromise the integrity of your in vivo research. Charles River offers TaqMan® PCR testing on the OpenArray® platform so you can perform precautionary screening on your research biologics to confirm that they are free of rodent infectious agents.

PCR Panels to Screen Cell Lines and Research Biologics for Rodent Infectious Agents

Agent Mouse Essential Panel Rat Essential PanelMouse/Rat

Comprehensive Panel

Murine norovirus (MNV) • •

Mouse parvoviruses* (MPV-1, MPV-2, MPV-3, MPV-4, MVM) • •

Mouse hepatitis virus (MHV) • •

Reovirus (Type 1 & 3) (REO) • • •

Lymphocytic choriomeningitis virus (LCMV) • • •

Lactate dehydrogenase-elevating virus (LDV) • • •

Mouse rotavirus (MRV/EDIM) • •

Theiler’s murine encephalomyelitis virus (TMEV [GDVII]) • • •

Mousepox (Ectromelia) (ECTRO) • •

Hantavirus hantaan (HANT) • •

Hantavirus seoul (SEO) • •

Polyoma virus (POLY) • •

K virus (K) •

Mouse adenovirus (MAV-1 & MAV-2) • •

Mouse cytomegalovirus (MCMV) •

Mouse thymic virus (MTLV) •

Pneumonia virus of mice (PVM) •

Sendai (SEND) • • •

Rat cytomegalovirus (RCMV) • •

Rat theilovirus (Theiler’s-like virus of rats [RTV]) • •

Rat parvoviruses* (RPV, KRV, RMV, H-1) • •

Rat rotavirus (IDIR) • •

Rat coronavirus (RCV, SDAV) • •

Mycoplasma (genus) (including Acholeplasma laidlawii) • • •

Mycoplasma pulmonis • • •

Positive template control • • •

Negative template control • • •

Spike inhibition control • • •

Nucleic acid recovery control (NARC) • • •

*Strain determination assays are performed on all positive results.

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Human CLEARTo help maintain the validity of your in vivo research, it is important to perform precautionary screening on your research biologics to confirm that they are free of infectious agents. Charles River provides TaqMan® PCR testing to identify human infectious agents in any of your research biologics. TaqMan® technology is ten to one hundred times more sensitive than traditional gel-based qualitative PCR, and the use of an internal probe provides incomparable specificity. This technology allows samples to be analyzed without opening reaction tubes, which prevents the release of potentially contaminating PCR products, a common downfall associated with gel-based PCR assays.

PCR Panels to Screen Cell Lines and Research Biologics for Human Infectious Agents

Contamination CLEARThe Cell Line Examination and Report (CLEAR) PCR Panel allows you to check the identity of your cell lines. It can detect inter-species contamination of less than 0.5% using TaqMan® PCR assays. Cells that can be differentiated include those originating from mouse, rat, Chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) species.

Agent Human HEP/HIV PanelHuman Essential

PanelHuman

Comprehensive Panel

Polyomavirus (John Cunningham virus) • •

Polyomavirus (BK virus) • •

Herpesvirus type 6 • •



Parvovirus B19 • •

Epstein-Barr virus • •

Hepatitis A virus • •

Hepatitis B virus • • •

Hepatitis C virus • • •

Papillomavirus type 16 • •

Papillomavirus type 18 • •

Human T-lymphotropic virus • •

Human cytomegalovirus • •

Human immunodeficiency virus type 1 • • •

Human immunodeficiency virus type 2 • • •

Adeno-associated virus • •

Human foamy virus • •

Mycoplasma (genus) (including Acholeplasma laidlawii) • • •

Lymphocytic choriomeningitis virus (LCMV) •

Hantavirus hantaan •

Hantavirus seoul •

Spike inhibition control • • •

Nucleic acid recovery control (NARC) • • •

Positive template control • • •

Negative template control • • •


14

Discovery ServicesCharles River provides discovery services with extensive experience in cancer pharmacology and specialty oncology models. Our broad range of models and support services allows clients to choose the most appropriate study design and screening method to identify promising compounds and optimize lead candidates.

• In Vitro Assays

• In Vivo Pharmacology Services:

- Human tumor xenografts

- Patient-derived xenografts

- Syngeneic models

- Orthotopic models

• Patient-Derived Human Tumor Grafts

• Integrated Drug Discovery

Collection of Oncology Research Experiments (CORE)The CORE (Collection of Oncology Research Experiments) is an online library of peer-reviewed publications designed to help you find the most appropriate research model for your oncology cell lines. Search through the publications by first selecting your tissue type.Simply select the cell line you want to reference and the publications will be listed by animal model. The publications were collected from various online resources and are non-curated. Visit www.criver.com/core for publications.

Supplemental ServicesCharles River’s global infrastructure gives you access to unparalleled products and services in the field of oncology. Whether you are in basic research and discovery or are ready to move your therapeutic into the clinical development phase, we are strategically positioned to help you accelerate your cancer research and anticancer drug development efforts.

Safety Assessment• General Toxicology Studies

• Infusion Toxicology Studies

• IND-Enabling Programs

• Pathology Services

• Drug Metabolism and Pharmacokinetics

• Laboratory Sciences

Clinical Support• Clinical Bioanalysis

• Clinical Pharmacokinetics

• Clinical Immunology and Biomarker Services

• Pathology for Clinical Trials

Manufacturing Support• GMP-Compliant Biologics Testing

• Endotoxin Detection and Microbial Identification

Documents

Charles River Immunodeficient Models Xenograft Data Catalog · Research Models Immunodeficient Models enograft Data Catalog - Volume 1 2 Fox Chase SCID® Congenic Mouse (C.B-17 SCID)