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BOI206 core course in B.sc of Applied Biology, USM, Malaysia
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Principles of Biochemistry BOI206
Lecture 1
Introduction to the Study of Cell
PRINCIPLES OF BIOCHEMISTRY (BOI206)
Compulsory
3 (3 x 1 h lecture per week)
42
Dr. Intan Haslina Ishak
Dr. Rashidah Abdul Rahim
Prof. Dr. K. Sudesh Kumar
Course:
Type:
Total Units:
No. of Lectures:
Lecturers:
Course outline 1. An introduction to biochemistry
2. Organisation of cell – Prokaryotic and eukaryotic cells.
3. Components of cells: Structure and function – Water and buffer, Acid amino and protein, Fatty acid and lipid, Monosaccharides and
carbohydrates, & DNA and RNA, Membrane and membrane transportation.
4. Enzymology – Enzyme as biological catalyst, Enzyme kinetics, Enzyme inhibitions, Enzyme regulation,
Allosteric enzymes.
5. Energetics and Metabolism – Principle of bioenergetics, Carbohydrate metabolism (glycolysis, citric acid cycle, pentose
phosphate pathway, gluconeogenesis), Photosynthesis, Oxidation in biology, Oxidation of fatty acids (lipid metabolism).
Objectives:
Course contents are divided into 4 major sections:
1. Concept of a cell: Prokaryote, Eukaryote, Animal and
Plant Cells; cellular organelles
2. Cell components (Structure & Function): Water and
buffer, amino acids and protein, fatty acids and lipid,
monosaccharide and carbohydrate, nucleotides and
DNA, RNA, membrane and membrane transport
Introduction to cells, organelles, molecules; structure &
function relationship; working of a cell
3. Enzymology: Enzyme as catalysts, enzyme kinetics,
inhibitor effects, enzyme regulations, allosteric enzymes
4. Energetics and metabolisms: Bioenergetic principles,
carbohydrate metabolisms (glycolysis), TCA cycle,
pentose phosphate pathway, gluconeogenesis and
photosynthesis, fatty acid oxidation
Course evaluation:
Laboratory Practical:
Test/Quiz/Assignment:
Tests:
Test/Quiz/Assignment/Practical: 50%
Final Exam: 50%
30%
20%
Test 1: Will be determined later
Test 2: Will be determined later
Come to class!!!!!!
• Review lecture notes. (Will be uploaded on elearn)
• Read book chapters. – Biochemistry, 5th edition, Campbell, M. K. & Farrel, S.O,
Thomson-Brooks/Cole.
– Principles of Biochemistry, 4th edition, Voet, D. & Voet, J.G., John Wiley & Sons, Inc.
Principles of
Biochemistry
4th Edition
Biochemistry
5th Edition
Biochemistry
• Chemistry of living organisms.
• The study of biology at the molecular level.
Scientists study cells from various perspectives such as:
1. Cytology: what we learn with a microscope
2. Genetics: what we learn using genetic techniques
3. Biochemistry: what we learn through biochemical
analysis
Characteristics of Life: All Living Things
1. Have metabolic activity; ability to acquire and use
energy
2. Use homeostatic controls which respond to environmental change
3. Show growth, development and reproduction
4. DNA is molecule of inheritance
5. Have adaptive potential; heritable variation in form,
function and behavior allow changes to accommodate
changing environment
6. Display diversity; variations in form, function and
behavior as a result of natural selection and
environmental change
biosphere tissue
ecosystem Cell: smallest
living unit community
population organelle
multicellular
organism
molecule
organ system atom
organ subatomic particle
Levels of Organization of Life
CLASSIFICATION: THE THREE DOMAIN SYSTEM
This system proposes that a common ancestor cell ("Cenancestor") gave
rise to three different cell types, each representing a domain. The three
domains are the Archaea (archaebacteria), the Bacteria (eubacteria), and
the Eukarya (eukaryotes). The Eukarya are then divided into 4 kingdoms:
Protists, Fungi, Animalia, and Plantae.
Eukarya
(eukaryotes)
The Three Domain System, proposed by Woese and others,
is an evolutionary model of classification based on:
i) differences in the sequences of nucleotides in the
cell's ribosomal RNAs (rRNA),
ii) the cell's membrane lipid structure
iii) its sensitivity to antibiotics.
Comparing rRNA structure is especially useful. Because
rRNA molecules throughout nature carry out the same
function, their structure changes very little over time.
Therefore similarities and dissimilarities in rRNA nucleotide
sequence are a good indication of how related or unrelated
different cells and organisms are.
The Bacteria and the Eukarya
have membranes composed of
unbranched fatty acid chains
attached to glycerol by ester
linkages.
The Archaea have membranes
composed of branched
hydrocarbon chains attached to
glycerol by ether linkages.
Prokaryotic cells are generally much smaller and more simple
than eukaryotic.
The smaller a cell, the greater is its surface-to-volume
ratio.
A large surface-to-volume ratio, as seen in smaller
prokaryotic cells, means that nutrients can easily and
rapidly reach any part of the cells interior.
In the larger eukaryotic cell, the limited surface area when
compared to its volume means nutrients cannot rapidly diffuse
to all interior parts of the cell.
That is why eukaryotic cells require a variety of specialized
internal organelles to carry out metabolism, provide
energy, and transport chemicals throughout the cell.
Both, have to carry out the same life biochemical processes.
Cells with a high metabolic rate are usually small.
Why?
Structure of virus: nucleic acid with a protein envelope
Virus: genetic parasite! Is it a cell?
T4 Bacteriophage: infects prokaryotes
Used by
scientists to
introduce
foreign genetic
material into
bacterial cells:
genetic
engineering/
recombinant
technology
Adenovirus (cold)
Nucleus
Cytoplasm
Plasma membrane
Protoplasm/protoplast
Cell wall
Plastid
Chloroplast
Nuclear plasma
Nucleolus
Chromatin
Important terms: Definitions? Nuclear membrane
Mitochondrion
Golgi apparatus/body
Cytosol
Endoplasmic reticulum
(smooth &
rough/granulated)
Centriole
Centrosome
Ribosome
Liposome
Vacuole
PROKARYOTE
Bacterial cell
Bacterial nucleoid (EM)
Bacterial spore
Bacterial (E. coli) flagellar
monotrichous
peritrichous
lophotrichous
amphitrichous
Locomotor organelles
Eukaryotic cell
May have flagella or cilia. Flagella and cilia are organelles
involved in locomotion and in eukaryotic cells consist of a
distinct arrangement of sliding microtubules surrounded by
a membrane. The microtubule arrangement is referred to
as a 2X9+2 arrangement.
Prokaryotic cell
Some have flagella, each composed of a single, rotating
fibril and not surrounded by a membrane. No cilia.
Transmission Electron
Micrograph of Escherichia coli
O157:H7
Flagella
composition: A bacterial flagellum has 3 basic parts: a filament, a hook, and a
basal body.
Structure of an eukaryotic cell
Structure of an eukaryotic cell
Plant Cell
Scytonemia (blue-green algae)
Mitochondria
Lysosome
Centriole & centrosome
Microfilament & Microtubule:
Cytoskeleton
Nucleus
Nuclear body
Eukaryotic cell
a. Bounded by a nuclear membrane having pores connecting
it with the endoplasmic reticulum
b. Contains one or more paired, linear chromosomes
composed of deoxyribonucleic acid (DNA) associated with
histone proteins
c. Nucleolus present.
d. Nuclear body is called a nucleus
Prokaryotic cell
a. Not bounded by a nuclear membrane
b. Usually contains one circular chromosome composed of
deoxyribonucleic acid (DNA) associated with histone-like
proteins.
c. No nucleolus.
d. Nuclear body is called a nucleoid
Chromosome
Cell division
Eukaryotic cell
a. By mitosis
b. Sex cells in diploid organisms are produced through
meiosis
Prokaryotic cell
a. Usually by binary fission. No mitosis.
b. Organisms are haploid. No meiosis needed.
Nucleic acid
Endoplasmic reticulum (smooth & rough)
EM
Rough endoplasmic reticulum (EM)
Golgi apparatus
Lipid assembly, polypeptide chain modification,
packaging of polypeptides for transport to other
various locations in cell
Plasma membrane
Cytoplasmic membrane (cell membrane, plasma
membrane)
Eukaryotic cell
a. Cytoplasmic membrane is a fluid phospholipid bilayer
containing sterols as well as carbohydrates.
b. Capable of endocytosis (phagocytosis and pinocytosis) and
exocytosis.
Prokaryotic cell
a. Cytoplasmic membrane; is a fluid phospholipid bilayer
without carbohydrates and usually lacking sterols . Many
bacteria do contain sterol-like molecules called hopanoids.
b. Incapable of endocytosis and exocytosis.
Size comparison of cellular components
Comparison of eukaryotic and prokaryotic cells and selected organels
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