CHAPTER 1 INTRODUCTION - Cole-Parmer · PDF fileChapter 1: Introduction provides a brief overview of ... Wiring diagrams for both the 115V and 230V units are ... DISCUSSION OF THE

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CCHHAAPPTTEERR 11IINNTTRROODDUUCCTTIIOONN

Congratulations on your purchase of a LabconcoPurifier Delta Series Biosafety Cabinet. Your Purifieris designed to protect you, your product and yourlaboratory environment from biohazardous aerosols. Itis the result of Labconco�s more than 25 yearsexperience of manufacturing biohazard cabinetry, andmany of its features were suggested to us by users likeyou.

The Purifier offers many unique features to enhancesafety, performance and ergonomics. To take fulladvantage of them, please acquaint yourself with thismanual and keep it handy for future reference. If youare unfamiliar with how biosafety cabinets operate,please review Chapter 4: Theory of Operation andSafety Precautions before you begin working in thecabinet. Even if you are an experienced biosafetycabinet user, please review Chapter 5: Using YourPurifier; it describes your Purifier�s features so that youcan use the cabinet efficiently.


Chapter 1: Introduction

About This ManualThis manual is designed to help you learn how toinstall, use, and maintain your biosafety cabinet.Instructions for installing optional equipment on yourcabinet are also included.

Chapter 1: Introduction provides a brief overview ofthe biosafety cabinet, explains the organization of themanual, and defines the typographical conventions usedin the manual.

Chapter 2: Prerequisites explains what you need to doto prepare your site before you install your biosafetycabinet. Electrical and service requirements arediscussed.

Chapter 3: Getting Started contains the informationyou need to properly unpack, inspect, install, andcertify your biosafety cabinet.

Chapter 4: Theory Of Operation And SafetyPrecautions explains how the Purifier operates and theappropriate precautions you should take when using thecabinet.

Chapter 5: Using Your Purifier discusses the basicoperation of your cabinet. Information on how toprepare, use and shut down your Purifier are included.

Chapter 6: Maintaining Your Purifier explains how toperform routine maintenance on your biosafety cabinet.Information on how to safely disinfect the interior ofyour cabinet and replace the lamps are included.

Chapter 7: Modifying Your Biosafety Cabinet describeshow to install the optional equipment on the cabinet.

Chapter 8: Troubleshooting contains a table ofproblems you may encounter while using yourbiosafety cabinet including the probable causes of theproblems and suggested corrective actions.

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Appendix A: Purifier Components contains labeleddiagrams of all of the components of the biosafetycabinets.

Appendix B: Biosafety Cabinet Dimensions containscomprehensive diagrams showing all of the dimensionsfor the 3, 4 and 6 foot models of the biosafety cabinet.

Appendix C: Biosafety Cabinet Specifications containsthe electrical requirements for the biosafety cabinet.Wiring diagrams for both the 115V and 230V units arealso included.

Appendix D: Purifier Accessories lists the part numberand descriptions of all of the accessories available foryour biosafety cabinet.

Appendix E: Quick Chart for the Delta Purifiersprovides useful operating specifications.

Appendix F: References lists the various resourcesavailable that deal with biosafety.

Typographical ConventionsRecognizing the following typographical conventionswill help you understand and use this manual:

• Book, chapter, and section titles are shown in italictype (e.g., Chapter 3: Getting Started).

• Steps required to perform a task are presented in anumbered format.

• Comments located in the margins providesuggestions, reminders, and references.

• Critical biosafety information is presented inboldface type in paragraphs that are preceded by thebiosafety icon. Failure to comply with theinformation following a biosafety icon may result inillness or death.

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• Critical information is presented in boldface type inparagraphs that are preceded by the exclamationicon. Failure to comply with the informationfollowing an exclamation icon may result in injuryto the user or permanent damage to your biosafetycabinet.

• Critical information is presented in boldface type inparagraphs that are preceded by the wrench icon.These operations should only be performed by atrained certifier or contractor. Failure to complywith the information following a wrench icon mayresult in injury to the user or permanent damage toyour biosafety cabinet.

• Important information is presented in capitalizedtype in paragraphs that are preceded by the pointericon. It is imperative that the information contained�

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in these paragraphs be thoroughly read andunderstood by the user.

• Information that is specific to a particular model ofBiosafety Cabinet is preceded by a number icon.The 3' icon indicates the text is specific to the 3-footwide model. The 4' icon indicates the text isspecific to the 4-foot model. The 6' icon indicatesthe text is specific to the 6-foot model.

Your Next StepIf your Purifier needs to be installed, proceed toChapter 2: Prerequisites to ensure your installation sitemeets all of the requirements. Then, go to Chapter 3:Getting Started for instructions on how to install yourbiosafety cabinet and make all of the necessaryconnections.

If you would like to review how biosafety cabinetsoperate, go to Chapter 4: Theory Of Operation AndSafety Precautions.

For information on the operational characteristics ofyour biosafety cabinet, go to Chapter 5: Using YourPurifier.

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If your biosafety cabinet is installed and you need toperform routine maintenance on the cabinet, proceed toChapter 6: Maintaining Your Purifier.

For information on making modifications to theconfiguration of your unit, go to Chapter 7: ModifyingYour Biosafety Cabinet.

Refer to Chapter 8: Troubleshooting if you areexperiencing problems with your Purifier.




CCHHAAPPTTEERR 22PPRREERREEQQUUIISSIITTEESS

Before you install your biosafety cabinet, you need toprepare your site for installation. Carefully examine thelocation where you intend to install your cabinet. Youmust be certain that the area is level and of solidconstruction. In addition, a dedicated source ofelectrical power must be located near the installationsite.

Carefully read this chapter to learn:

• the location requirements for your installationsite.

• the electrical power requirements for yourinstallation site.

• the exhaust requirements for your installationsite.

• the service line requirements for yourinstallation site.

• the space requirements for your installation site.

Refer to Appendix C: Biosafety Cabinet Specificationsfor complete biosafety cabinet electrical andenvironmental conditions, specifications andrequirements.

8

Chapter 2: Prerequisites

Location RequirementsThe Purifier should be located awayfrom traffic patterns, doors, fans,ventilation registers, fume hoods and

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any other air-handling device thatcould disrupt its airflow patterns. Allwindows in the room should be closed.Figure 2-1 shows the optimumlocation for the Purifier.

DO NOT locate the cabinet on a cart,dolly, or mobile bench. ALL Purifierinstallations must be permanent andstationary.

haust RequirementsIF YOU INTEND TO CONNECTYOUR PURIFIER TO EITHER THEOPTIONAL CANOPY OR B3

Figure 2-1


CONNECTION KITS, FIRST



EXAMINE THE LOCATION TOENSURE THAT IT IS COMPATIBLEWITH THE CABINET�S EXHAUSTDUCT. THE AREA DIRECTLYABOVE THE CABINET�S EXHAUSTPORT SHOULD BE CLEAR OFSTRUCTURAL ELEMENTS, WATERAND UTILITY LINES, OR OTHERFIXED OBSTRUCTIONS. THERESHOULD BE ENOUGH CLEARANCETO ALLOW FOR THE PASSAGE OFA 10" PVC DUCT. AVOID CABINETLOCATIONS THAT REQUIREEITHER AN ELBOW DIRECTLY ONTOP OF THE CABINET�S EXHAUSTCONNECTION OR AN EXCESSIVENUMBER OF ELBOWS TO CLEAROTHER ITEMS. FOR A FURTHERDISCUSSION OF THE CABINET�SEXHAUST SYSTEMREQUIREMENTS, PLEASE GO TOChapter 3: Getting Started.

Electrical RequirementsThe different Purifier models have the followingelectrical requirements:

Model # Requirements36204- 00-09 115 VAC, 60 Hz, 12 Amps36205- 00-09 115 VAC, 60 Hz, 12 Amps36205- 10-19 100 VAC, 50 / 60 Hz, 12 Amps36205- 20-29 230 VAC, 50 Hz, 6 Amps

36208- 00-09 115 VAC, 60 Hz, 12 Amps36209- 00-09 115 VAC, 60 Hz, 12 Amps36209- 10-19 100 VAC, 50 / 60 Hz, 12 Amps36209- 20-29 230 VAC, 50 Hz, 6 Amps

36212- 00-09 115 VAC, 60 Hz, 16 Amps36213- 00-09 115 VAC, 60 Hz, 16 Amps36213- 10-19 100 VAC, 50 / 60 Hz, 16 Amps36213- 20-29 230 VAC, 50 Hz, 8 Amps

Table 2-1

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All Purifiers with model numbers ending in �00 to �09are designed for operation at 115 volts 60Hz,alternating current. Models with numbers ending in �10 to �19 are designed for operation at 100 to 120 volts50/60Hz. Purifiers with model numbers ending in �20to �29 are designed for operation at 230 +/- 20 volts, 50Hz alternating current. A dedicated outlet with a circuitbreaker rated at 20 amps should be located as close aspossible to the right rear side of the cabinet, at a heighteven with, or higher than, the bottom of the cabinet inits final location. If your electrical outlet is distant fromthis location, contact Labconco�s Product ServiceDepartment for information on longer cords.

Always follow the plugmanufacturer�s instructions for theproper assembly and testing of the

SeAll outsaccrequmusthe

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plug and power cord.

BOTH ELECTRICAL OUTLETS OFTHE 100 AND 115 VOLT PURIFIERARE PROTECTED BY A GROUND
FAULT INTERRUPTER CIRCUIT(GFIC). LABCONCO DOES NOTRECOMMEND INSTALLING A GFICON THE OUTLET THAT THEPURIFIER PLUGS INTO.
rvice Line Requirementsservice lines to the Purifier should be ¼ inchide diameter, metal, and equipped with an easily

essible shut-off valve, should disconnection beired. If the service line pressure exceeds 40 PSI, itt be equipped with a pressure regulator to reduceline pressure.

The use of flammable gases or solventsshould be avoided in the Purifier.Open flames in the cabinet will


disrupt the laminar airflow in thecabinet and may damage the HEPA



filters. Flammable gases or solventsmay reach explosive concentrations inthe cabinet or ductwork. If you feelthat your procedure requires the useof an open flame or flammablematerials, contact the appropriatesafety official.

The use of air or gases under highpressure should be avoided as theymay seriously disrupt the airflowpatterns in the cabinet.

Space RequirementsThe dimensions for the different models are shown inAppendix B: Biosafety Cabinet Dimensions.

Your Next StepAfter you have determined that the location you haveselected accommodates the installation and operationalrequirements of your biosafety cabinet, you are ready tobegin installation. Proceed to Chapter 3: GettingStarted.



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CCHHAAPPTTEERR 33GGEETTTTIINNGG SSTTAARRTTEEDD

Now that the site for your biosafety cabinet is properlyprepared, you are ready to unpack, inspect, install, andcertify your unit. Read this chapter to learn how to:

• unpack and move your Purifier.• set up the cabinet.• connect the electrical supply source.• connect the service lines.• connect to an exhaust system (optional).• arrange certification of your Purifier.

Depending upon which model you are installing, youmay need common plumbing and electrical installationtools in addition to two 1/2" wrenches, a 7/16" wrench,a flat-blade screwdriver, a Phillips screwdriver, and acarpenter level to complete the instructions in thechapter.

The Purifier models weigh between400 � 700 lbs. (182-318 kg). Thecarton allows for lifting with a!

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mechanical lift truck or floor jack. Ifyou must lift the Purifier manually,use at least six (6) persons and followsafe-lifting guidelines.

14

Chapter 3: Getting Started

Unpacking Your BiosafetyCabinetCarefully unpack your Purifier and inspect it fordamage that may have occurred in transit. If your unitis damaged, notify the delivery carrier immediately andretain the entire shipment intact for inspection by thecarrier.

DO NOT RETURN GOODSWITHOUT THE PRIORAUTHORIZATION OF LABCONCO.UNAUTHORIZED RETURNS WILLNOT BE ACCEPTED.

IF YOUR CABINET WAS DAMAGEDIN TRANSIT, YOU MUST FILE ACLAIM DIRECTLY WITH THEFREIGHT CARRIER. LABCONCOCORPORATION AND ITS DEALERSARE NOT RESPONSIBLE FORSHIPPING DAMAGES.

Do not discard the carton or packing material for yourPurifier until you have checked all of the componentsand installed and tested the unit.

Do not remove the Purifier from its shipping skid untilit is ready to be placed into its final location. Move theunit by placing a flat, low dolly under the shipping skid,or by using a floor jack.

Do not move the cabinet by tilting itonto a hand truck.

The United StatesInterstate CommerceCommission rulesrequire that claims befiled with the deliverycarrier within fifteen (15)days of delivery.

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Purifier ComponentsLabconco manufactures biosafety cabinets for operationat an 8-inch or 10-inch sash opening. Each of thesecabinets is available in a 3-foot, 4-foot and 6-footmodel. Models are available in 115V, 100V or 230V.

Locate the cabinet model you received in the followinggroup of tables. Verify that the components listed arepresent and undamaged.

Catalog # Purifier Description36204-00 3 foot Purifier, 10 inch sash opening, 115 VAC36204-04 3 foot Purifier with UV lamp & fixture, 10 inch sash opening, 115 VAC36205-00 3 foot Purifier, 8 inch sash opening, 115 VAC36205-04 3 foot Purifier with UV lamp & fixture, 8 inch sash opening, 115 VAC36205-10 3 foot Purifier, 8 inch sash opening, 100 VAC 50/60 Hz36205-14 3 foot Purifier with UV lamp and fixture, 8 inch opening, 100 VAC 50/60 Hz36205-20 3 foot Purifier, 8 inch sash opening, 230 VAC36205-24 3 foot Purifier with UV lamp & fixture, 8 inch sash opening, 230 VAC

36208-00 4 foot Purifier, 10 inch sash opening, 115 VAC36208-04 4 foot Purifier with UV lamp & fixture, 10 inch sash opening, 115 VAC36209-00 4 foot Purifier, 8 inch sash opening, 115 VAC36209-04 4 foot Purifier with UV lamp & fixture, 8 inch sash opening, 115 VAC36209-10 4 foot Purifier, 8 inch sash opening, 100 VAC 50/60 Hz36209-14 4 foot Purifier with UV lamp and fixture, 8 inch opening, 100 VAC 50/60 Hz36209-20 4 foot Purifier, 8 inch sash opening, 230 VAC36209-24 4 foot Purifier with UV lamp & fixture, 8 inch sash opening, 230 VAC

36212-00 6 foot Purifier, 10 inch sash opening, 115 VAC36212-04 6 foot Purifier with UV lamp & fixture, 10 inch sash opening, 115 VAC36213-00 6 foot Purifier, 8 inch sash opening, 115 VAC36213-04 6 foot Purifier with UV lamp & fixture, 8 inch sash opening, 115 VAC36213-10 6 foot Purifier, 8 inch sash opening, 100 VAC 50/60 Hz36213-14 6 foot Purifier with UV lamp and fixture, 8 inch opening, 100 VAC 50/60 Hz36213-20 6 foot Purifier, 8 inch sash opening, 230 VAC36213-24 6 foot Purifier with UV lamp & fixture, 8 inch sash opening, 230 VAC

Plus the Following:

Part # Component Description37371-00 User�s Manual

Drain Valve AssemblyPower Cord

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If you did not receive one or more of the componentslisted for your Purifier, or if any of the components aredamaged, contact Labconco Corporation immediatelyfor further instructions.

Removing the ShippingSkid

LEAVE THE PURIFIER ATTACHEDTO ITS SHIPPING SKID UNTIL IT ISAS CLOSE TO ITS FINAL

AcTsh

T

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2

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LOCATION AS POSSIBLE. MOVETHE UNIT BY USING A SUITABLEFLOOR JACK, OR BY PLACING AFURNITURE DOLLYUNDERDNEATH THE SKID. DONOT MOVE THE CABINET BYTILTING IT ONTO A HAND TRUCK.
fter you verify the Purifier components, move yourabinet to the location where you want to install it.hen, follow the steps listed below to remove theipping skid from your unit.

o remove the shipping skid:

The side panels of the Purifier areheavy; exercise caution whenremoving and installing them.

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Remove the plastic wrapping and corner posts.Remove the packaging material from the top of thecabinet.Remove both side panels by loosening and keepingthe screws and 3 acorn nuts that secure each panelto the Purifier, as shown in Figure 3-2.Remove and discard the four screws that secure theunit to the skid, as shown in Figure 3-1.Remove the two sash retaining screws and bracketsfrom the bottom edge of the sash. Discard thescrews and brackets.



Figure 3-1

Figure 3-2

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5. Examine the sash cables at both upper front cornersof the Purifier, as shown in Figure 3-3. The cablesshould rest securely in the pulley.

Ensure that both sash cables areproperly oriented before proceeding.Releasing the sash weights with the

6.

7.

8.

9.

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cables improperly positioned candamage the cables, pulleys or its axle.

Locate the weight restraining bolt in each weightchannel. It is a ¼ inch diameter bolt that runs fromthe front through to the rear of the channel. Itslocation in the channel will vary from model tomodel. Remove each bolt while supporting the sashcable, ensuring that it is seated in the pulley. Savethe bolts for future use if the unit is to betransported.Install the two sash stop screws and spacers on thefront of the cabinet, as shown in Figure 3-4.Raise and lower the sash to check it for properoperation. The sash should move smoothly. If thesash binds or requires excessive force to operate,examine the cables and pulley for proper operation.Examine the right weight track as shown in Figure3-3. When the sash is completely closed and restingagainst the sash stop, the top surface of the weightshould be 1 5/8" (41 mm) below the top of theweight channel. If it is not, adjust the weightchannel by loosening the six nuts that secure thechannel to the side wall of the cabinet. Slide theweight channel up or down until the dimension isproper. Retighten all six nuts to secure the channel.



Figure 3-3

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Installing the Purifier On AnExisting Work Surface

The Purifier is very top heavy! Usecaution when lifting or moving theunit.

Whsurfsuprelaas wsup

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Figure 3-4


en installing the Purifier onto an existing workace or benchtop, ensure that the structure can safelyport the combined weight of the cabinet and anyted equipment. The work surface should be at leastide as the unit and 31 inches deep to properly

port the unit.



The work surface should be smooth and durable. Thesurface should be nonporous and resistant to thedisinfectants and chemicals used in conjunction withthe Purifier. These criteria will allow a proper seal toform between the bottom of the Purifier and the worksurface.

A hole or notch may need to be cut in some worksurfaces in the right front corner to accommodate thedrain valve.

Installing the Purifier on theLabconco Base StandLabconco offers Base Stands as an option to support thePurifier, which are to be purchased separately. Thestands are adjustable from a working (work surface)height of 30 inches (76.2 cm) to 36 inches (91.4 cm) in1 inch (2.5 cm) increments. The base stand for eachPurifier model is listed in Table 3-1 below.

Purifier Model # Base Stand Model #36204-xx, 36205-xx 37303-0036208-xx, 36209-xx 37304-0036212-xx, 36213-xx 37306-00

For further information regarding Purifier Base Standsor other accessories, please contact Labconco�sCustomer Service Department at 1-800-821-5525 or816-333-8811 weekdays, between the hours of 7:00a.m. and 6:00 p.m. CST.

Table 3-1



1. Before positioning the Purifier Base Stand in itsfinal location, decide the final height of the stand.The height of the work surface can be anywherebetween 30 to 36 inches.

2. Select the height of the stand and slide four (4) legextensions into base stand corner posts and attachwith 2 ¼ inch long bolt, flatwasher, lockwasher andnut. Ensure that the same height hole is selected foreach leg. Tighten the leg bolts securely. See Figure3-5.

3. Move the base stand into its final location. Using acarpenter�s level, adjust each leveling foot until thestand is level in both planes as shown in Figure 3-6.You are now ready to lift the Purifier onto its stand.

Figure 3-5

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The Purifier is very top heavy! Usecaution when lifting or moving it.

4.

5.

6.

7. 8.

Figure 3-6

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Carefully lift the Purifier onto the top rails of thebase stand and slide it into position.Using a drift punch, or a medium sized screwdriver,align one of the four holes in the bottom flange ofthe Purifier with its matching hole in the base stand.Insert the 5/16-inch mounting bolt through the holeand secure it with a flatwasher, lockwasher andacorn nut.Repeat steps 5 and 6 for the remaining bolts.Tighten all four bolts securely.

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Connecting The Purifier toService LinesThe service lines (if any) should be connected to thetube fitting(s) on the outside of the liner wall as shownin Figure 3-7. To install the tubing, follow these steps:

1. Ensure that the tubing is ¼ inch outside diameter,soft metal, and that the end has been deburredcompletely.

2. Route the tubing from the rear of the cabinet,ensuring that it will line up with the slot in the backof the side panel.

Make sure that the tube routing willnot contact any electrical wires.

3.

4.

5.

6.

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If routing tubing to the front valveposition, route the tubing under thesash weight track and at the bottomedge of the side panel. Ensure that thetubing will not interfere with the sashweight when the sash is raised orlowered.

Make sure that the tube fitting nut on the fitting isloose, but do not remove it. Look inside the fittingto make sure the tube ferrule is there.Push the tube into the fitting until it is properlyseated. The tube will go approximately ¾ inch intothe fitting.Tighten the tube fitting nut hand tight and then,using a 7/16 inch wrench, tighten it at least ¾ turnmore.Close the service valve in the Purifier and thenslowly open the shutoff valve on the service valve.Inspect the fitting for leakage. Tighten the tube nutslightly if needed.

Product S


Optional Exhaust SystemConnectionsUnless specially modified by Labconco at your request,all Purifier Delta Series Biosafety Cabinets are shippedin the �Type A mode,� meaning they recirculate theirHEPA filtered exhaust air back into the laboratory.Certain applications such as working with odorousproducts or volatile toxic materials will require theconnection of your Purifier to an exhaust system. Thissection contains specific information on both theseexhaust systems and their requirements.

THE CANOPY CONNECTION, ALSOREFERRED TO AS A THIMBLE ORAIR GAP CONNECTION, ALLOWS

Figure 3-7

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SINGLE OR MULTIPLEBIOHAZARD CABINETS TO BECONNECTED TO AN EXHAUSTSYSTEM. DURING OPERATION,

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THE EXHAUST SYSTEM DRAWSALL OF THE CABINET�S EXHAUSTAIR, PLUS A VOLUME OF ROOMAIR (THROUGH THE HOLES ORSLOTS IN THE CANOPY) INTO THEEXHAUST DUCT. CANOPYCONNECTIONS OFFER THEADVANTAGE OF GANGINGMULTIPLE CABINETS TOGETHER(IF PROPERLY BALANCED) TO ASINGLE EXHAUST. THE CANOPYALSO FUNCTIONS AS A �SHOCKABSORBER� ALLOWING THESYSTEM TO FUNCTION PROPERLYDURING CHANGES IN ROOM AIRPRESSURE. THE CANOPYCONNECTION IS DESIGNEDPRIMARILY FOR WORKINVOLVING NON-TOXIC ODOROUSMATERIALS.

THE TYPE B3 CONNECTION IS ASEALED EXHAUST SYSTEM FROMTHE TOP OF THE CABINET TO THE�


REMOTE BLOWER. TYPE B3EXHAUST SYSTEMS SHOULD BEDEDICATED TO A SINGLECABINET, AND THE CABINETMUST BE EQUIPPED WITH ANEXHAUST FLOW ALARM, SUCHTHAT AN ALARM SOUNDS, ANDTHE CABINET BLOWERS SHUTOFF IN THE EVENT OFINSUFFICIENT EXHAUST FLOW.THE B3 CONNECTION ALLOWSTHE PURIFIER TO BE USED WITHVOLATILE TOXIC CHEMICALS ORRADIONUCLIDES, PROVIDED THERECIRCULATION OF VOLATILECOMPOUNDS IN THE WORK AREAWILL NOT INTERFERE WITH THEWORK.

Product Serv


If your research involves the use of toxic compounds orvolatile materials, contact your facility�s safety officeror Labconco to ensure that your Purifier and its exhaustsystem are compatible with the materials you will beworking with.

Canopy Connection KitThere are three Canopy Connection Kits available forPurifiers. They are:

Canopy Kit Number Purifier Width37307-00 3 feet*37307-01 4 feet37307-02 6 feet

Verify the kit you have ordered is thecorrect one for your Purifier.

ExReThePVCsizeshogreawat

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Table 3-2

haust Systemquirements

Canopy Connection Kit is sized to accept 10-inch ductwork. The exhaust system�s blower should be

d to handle the exhaust volume of each cabinet, aswn in Table 3-3, with a static pressure equal to orter than the system pressure, plus 0.2 inches of

er.

The exhaust system should be fittedwith a backdraft damper to preventthe reversing of airflow in the system.

*On models with serial numbers beginning in 99xx, 0001 through 0007, contact Labconco at 1-800-821-5525for further details.

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LABCONCO MANUFACTURES ANEXHAUST BLOWER WITHINTEGRAL BACKDRAFT DAMPER,

ModelNumber

AvVe

36204-0x36205-0x36205-1x36208-0x36209-0x36209-1x36209-2x36212-0x36213-0x26213-1x36213-2x

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PART NUMBER 36680-00, THAT ISSUITABLE FOR MOST SINGLECABINET INSTALLATIONS. THEREMOTE BLOWER IS DISCUSSEDFURTHER IN APPENDIX D.
Purifier Airflow Dataerage Inflowlocity (FPM)

Work OpeningArea (sq. ft.)

Exhaust Volume ofCabinet (CFM)

Exhaust Volume ofThimble (CFM)

105 2.53 253-278 278-306105 2.03 203-223 223-245105 2.03 203-223 223-245105 3.37 337-370 371-408105 2.69 269-296 296-325105 2.69 269-296 296-325105 2.69 269-296 296-325105 5.03 503-554 553-609105 4.03 403-443 443-488105 4.03 403-443 443-488105 4.03 403-443 443-488

nstalling the Canopy KitThe Canopy Kit and its connection tothe exhaust duct should be installedby a qualified certifier or contractor.

Table 3-3

Installing a canopy connection andexhaust system will impact theperformance of the Purifier. Do notattempt to use the cabinet until it hasbeen properly certified as described inthis chapter.

Use caution when removing orinstalling components on the exhaustfilter. When exposed, the surface of!


the HEPA filter is very fragile and canbe damaged easily. If you suspect that



the exhaust filter has been damagedduring the installation of the canopy,DO NOT use the cabinet; have itcertified by a qualified certifier beforeusing it.

1. Unplug the unit.2. Some early 3-foot wide Purifiers (serial numbers

beginning with 99xx, 0001 through 0007) wereequipped with a single piece, orange exhaust coveras shown in Figure 3-8. Contact Labconco at 1-800-821-5521 for details on conversion of theseunits.

Later model 3-foot, 4-foot and 6-foot wide Purifiersutilize a two piece exhaust cover system. Removethe four nuts that secure the orange exhaust diffusercover from the top of the Purifier, as shown inFigure 3-9. Remove the diffuser cover but leave theprimary exhaust control cover in place, as shown inFigure 3-9.

3. Install the canopy connection on the top of thePurifier, ensuring that the canopy slot faces thefront of the unit, as shown in Figure 3-10. Reinstallthe four nuts that were used to secure the exhaustdiffuser, and tighten them securely.

4. Connect the exhaust stack to the exhaust system,preferably using a flexible duct connection, sizedfor 10-inch PVC duct.

Balancing of airflows in the cabinet isaccomplished by adjustment of thecabinet�s exhaust control system andspeed control. The exhaust systemshould be considered a separatesystem, distinct from the Purifier.Changing the damper setting or thespeed control will affect the inflow anddownflow values. Both must bechecked after any adjustment.

3'

3' 4' 6'

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Downflow and inflow values andmeasurement procedures for thePurifier are described fully in Chapter6: Maintaining Your Purifier.

After adjusting the cabinet inflow anddownflow to specifications, the airflowin the exhaust duct can be determined

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in two ways.

By calculating the Total Exhaustvolume and subtracting either thecanopy inflow volume or the cabinetinflow volume from it.

OR

By adding the canopy inflow volumeand the cabinet inflow volume,yielding the Total Exhaust volume.

easuring the Canopynflow Volumepen the exhaust damper until the following average

irflows are noted flowing into the center of the canopyot:

Cabinet Width Average Inflow (FPM)36204 514-57636205 411-45336208 699-78236209 555-59736212 1029-113136213 823-926

Table 3-4



Measuring the TotalExhaust VolumeMeasure the airflow in the exhaust duct in accordancewith American Society of Heating, Refrigerating andAir-Conditioning Engineers (ASHRAE) recommendedprocedures. It should correspond to the airflow valuesshown in Table 3-3.

Measuring the CabinetInflow VolumeMeasuring the cabinet inflow volume is covered inChapter 6: Maintaining Your Purifier.

Figure 3-8



Figure 3-9

Figure 3-10

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Type B3 Connection KitThere are three Type B3 Connection Kits available forPurifiers. They are:

Canopy Kit Number Purifier Width37308-00 3 feet37308-01 4 feet37308-02 6 feet

Verify the kit you have ordered is thecorrect one for your Purifier.

ExReThePVCsize

shogreawat

Table 3-5

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haust Systemquirements

Type B3 Connection Kit is sized to accept 10-inch ductwork. The exhaust system�s blower should be

d to handle the exhaust volume of each cabinet, as

wn in Table 3-6, with static pressure equal to orter than the system pressure plus 0.2 inches of

er.

The exhaust system should be fittedwith a backdraft damper to preventthe reversing of airflow in the system.

LABCONCO MANUFACTURES ANEXHAUST BLOWER WITHINTEGRAL BACKDRAFT DAMPER,

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PART NUMBER 36680-00, THAT ISSUITABLE FOR MOST SINGLECABINET INSTALLATIONS. THEREMOTE BLOWER IS DISCUSSEDFURTHER IN Appendix D: PurifierAccessories.

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Purifier Airflow DataModel

NumberAverage InflowVelocity (FPM)

Work OpeningArea (sq. ft.)

Exhaust volume ofCabinet (CFM)

Alarm SetpointVolume (CFM)

36204-0x 105 2.53 253-278 228 ±536205-0x 105 2.03 203-223 183 ±536205-1x 105 2.03 203-223 183 ±536205-2x 105 2.03 203-223 183 ±536208-0x 105 3.37 337-370 303 ±536209-0x 105 2.69 269-296 242 ±536209-1x 105 2.69 269-296 242 ±536209-2x 105 2.69 269-296 242 ±536212-0x 105 5.03 503-554 453 ±536213-0x 105 4.03 403-443 363 ±536213-1x 105 4.03 403-443 363 ±536213-2x 105 4.03 403-443 363 ±5

Installing the Type B3 KitThe Type B3 Kit and its connection tothe exhaust duct should be installedby a qualified certifier or contractor.

Installing a Type B3 connection andexhaust system will impact theperformance of the Purifier. Do notattempt to use the cabinet until it hasbeen properly certified as described inthis chapter.

Use caution when removing orinstalling components on the exhaustfilter. When exposed, the surface of

Table 3-6

!


the HEPA filter is very fragile and canbe damaged easily. If you suspect thatthe exhaust filter has been damaged,DO NOT use the cabinet; have itcertified by a qualified certifier beforeusing it.



1. Unplug the unit.2. Some early 3-foot wide Purifiers were equipped

with a single piece, orange exhaust cover, as shownin Figure 3-8. Remove this cover by removing thesix acorn nuts that secure it to the top of thePurifier. Lift the cover straight up.

Later 3-ft units, as well as all 4-foot and 6-foot widePurifiers utilize a two piece exhaust cover system.Remove the four nuts that secure the orange exhaustdiffuser cover from the top of the Purifier. Using a7/16" wrench, remove the primary exhaust controlcover. Using the same wrench, remove the twoangled stiffeners on either side of the exhaust filteroutlet opening, as shown in Figure 3-11.

3. Carefully locate the exhaust connector on top of thecabinet, ensuring the exhaust damper is orientedtowards the front of the cabinet. Reinstall the sixlockwashers and acorn nuts that were used to securethe angled stiffeners on the top of the unit.

4. Connect the exhaust stack to the exhaust system,preferably using a flexible duct connection, sizedfor 10-inch PVC duct.

5. Remove the two acorn nuts that secure theelectronics module to the top of the Purifier. Tiltthe electronics module onto its rear surface.

6. Locate the speed control board. On the board,locate the red 8-wire electrical connector, identifiedon the board as �J5�, as shown in Figure 3-12. Notethat the wiring harness has a separate 8-pinconnector that plugs into a mating 8-pin connectorthat is soldered to the PCB.

7. Locate the yellow jumper wire on J5. If this yellowwire has two mating connectors on it, unplug theseconnectors and connect the wire harness #37527that is supplied with the B3 Kit as described in step8A. If the yellow wire has no connectors and is justa piece of wire that loops around to J5, mark theposition of each end of the wire on the connectorwith a marking pen. Carefully remove both ends ofthe wire from the connector by pulling straight upon each end of the wire. Connect the wire harness#37527 that is supplied with the B3 Kit as describedin step 8B.

3' 4' 6'

3'



8. A) Locate the two switch wires (harness #37527)coming out of the switch enclosure located on theside of the exhaust stack. Route the wires along thewiring harness entering the right side of theelectronics module. Plug in the terminals on theend of these two wires (from the switch enclosure)to the connectors on the yellow wires that connectto J5 (see step 7 above).B) Locate the two switch wires (harness #37527)coming out of the switch enclosure located on theside of the exhaust stack. Route the wires along thewiring harness entering the right side of theelectronics module. If the wires included with theB3 Kit have terminals on them, cut these terminalsoff. Press the end of each wire into the positions inthe J5 connector that the yellow jumper wireoccupied. The orientation of the wires on theconnector does not matter. Use a small screwdriverto drive the wires into the connector, forming agood electrical connection.

9. Secure the electronics module back to the top of thePurifier. The unit is now ready to be certified.

Balancing of the exhaust flow isaccomplished by adjusting theconnection kit�s exhaust damper.Changing the damper setting or the

speed control will affect the inflow anddownflow values. Both must bechecked after any adjustment.

Downflow and inflow values andmeasurement procedures aredescribed fully in Table 3-6, andChapter 6: Maintaining Your Purifier.

Calibration of the FaceVelocity Alarm

A qualified certification technicianshould calibrate the Face Velocity



Alarm. The alarm switch is set at thefactory at its lowest pressure setting,and it will need to be calibrated beforeusing the Purifier.

1. Set the exhaust damper so that the exhaust volumeis at the alarm setpoint as indicated in Table 3-6,and mark the damper position.

2. With the Purifier running, turn the adjusting screw,as shown in Figure 3-13 very slowly clockwise(increasing the pressure setting) until the alarm isactivated. This is the alarm setpoint.

3. Confirm the alarm operation by opening the damperslightly and pressing the �Alarm Silence� switch.The cabinet will operate normally. While thecabinet is running, slowly return the damper to itssetpoint. If the alarm does not sound at the setpoint,repeat steps 2 and 3 until proper operation isobtained.

4. Open the exhaust damper until the exhaust volumeis at the normal exhaust volume, as indicated inTable 3-6.

5. With the exhaust volume set, the downflow can beestablished as described in Chapter 6: MaintainingYour Purifier.

Figure 3-11



Figure 3-12

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Drain Valve InstallationIn order to prevent damage during shipping, the drainvalve assembly has not been installed in the unit. Ifdesired, the valve should be installed after the unit is inits final location. To install the valve assembly, followthese steps:

The work surface is heavy. Usecaution when handling it.

1.

!

Figure 3-13

39ice 1-800-522-7658

The six foot work surface is a largepart. Use two people and properlifting technique when removing itfrom the cabinet.

Lift the work surface out of the Purifier byremoving the thumbscrews at the front of the worksurface and loosening those at the rear of the pan.

6'



2. Using a putty knife, carefully remove and discardthe stainless steel cover that is sealed over the drainmounting holes. Scrape any remaining sealant thatis around the holes.

3. Apply a light coating of the silicone sealant to themounting surface of the drain assembly. Attach thedrain assembly as shown in Figure 3-13. Wipe offany excess sealant from the cabinet bottom. Ensurethat the center drain hole is unobstructed.

4. Make sure the drain valve is in the closed position.5. Reinstall the work surface.6. Allow the silicone sealant to cure for at least eight

hours before exposing it to liquid.

Figure 3-14



Initial CertificationPrior to use, all Purifier cabinets should be certified bya qualified certifier. Under normal operatingconditions, the Purifier cabinet should be recertified atleast annually, and when moved or serviced. Thecertifier should perform the following tests, asrecommended in NSF International Standard Number49:

• Downflow Velocity Profile Test• Inflow Velocity Test• Airflow Smoke Patterns• HEPA Filter Leak Test• Electrical Leakage and Ground Circuit

Resistance Test• Vibration Test *• Noise Level Test *• Lighting Intensity Test *

*These tests are user comfort relatedtests and may be omitted at the user�s orcertifier�s discretion.

If you have any questions regarding certificationagencies or need assistance in locating one, contactLabconco�s Product Service Department at 1-800-522-7658 or 816-333-8811.

Your Next StepAfter your Purifier has been installed and certified, youare ready to proceed to Chapter 4: Theory Of OperationAnd Safety Precautions.




CCHHAAPPTTEERR 44TTHHEEOORRYY OOFF

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All biohazard cabinets operate using the followingprinciples:

• Filtration and retention of particulates by HighEfficiency Particulate Air (HEPA) filter(s)

• Laminar airflow (sometimes)• Directional airflow

The major components in a Biohazard Cabinet are:

• The HEPA filter(s)• The motor/blower to force air through the unit• A speed control for the motor• Cabinet air intakes (grilles), ductwork and air

balance controls

44

Chapter 4: Theory Of Operation And Safety Precautions

HEPA FiltersHEPA filters are disposable, dry-type particulate filters.The filter material or media is typically made ofborosilicate microfibers that are made into a thin sheet,in a process similar to the production of paper. Thissheet is folded, or pleated to increase its surface area.The pleats are held in place by aluminum diffusers orby beads of glue that add rigidity to the media pack.The media pack is then set into a suitable frame, and theperimeter sealed to the filter frame, as shown in Figure4-1.

The HEPA filter media is very fragile.Do not touch or contact the mediasurface. If you think the surface of aHEPA filter is damaged, DO NOTUSE THE CABINET. Have theHEPA filter integrity tested by aqualified certifier before using thecabinet.

HEPA Filters are only effectiveagainst particulate material. Gaseswill pass through the filter.!




Laminar AirflowLaminar airflow is defined as the movement of a bodyof air in a single direction, with a uniform velocity. Inpractice, the laminar downflow of air in the cabinetcaptures any aerosol generated in the work area of thecabinet, and directs it to the HEPA filters. In order to betrue laminar downflow, a number of individualdownflow velocity test points (The Downflow VelocityProfile) must be +/- 20 % of the average of all the testpoints. This is shown graphically in Figure 4-2.

Figure 4-1



Directional AirflowDirectional airflow also plays a key role in biosafetycabinet performance. Air is drawn into the front of thecabinet at the front grille. This �curtain� of air makes itmore difficult for aerosols to escape out of the workarea of the cabinet and into the outside environment.This airflow is often calculated and referred to as theInflow Volume or Average Inflow Velocity. This isshown graphically in Figure 4-3.

Figure 4-2



Motor/BlowerThe motor/blower assembly pulls air into the front ofthe cabinet, and recirculates it internally. During itsrecirculation, the air is split into two separate streams.One path leads through the exhaust HEPA filter and outof the unit. The second path flows through the supplyHEPA filter, which then flows down through the workarea.

Figure 4-3

48


Speed ControlThe speed control should only beadjusted by a qualified certifier.

The speed control is an electronic circuit that allows thecertifier to set the motor speed by adjusting its voltage.The control will 'hold' this voltage, as line voltagefluctuates, allowing for stable operation. ThePurifier speed control is rated for 25 Amps current, farin excess of the motor's normal current draw, to allowfor greater reliability.

Cabinet Air Intakes (Grilles),Ductwork and Air BalanceControlsCabinet containment and performance are affected bythe location, size, and pattern of the grilles at the frontand rear of the work area.

Never block or obstruct the grilles ofthe Purifier.

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internal ductwork of the Purifier conveys the air the work area to the blower, and then from theer to the filters. The positive pressure plenum of

Purifier is unique in the industry, utilizing perfectifold technology to deliver a more uniform airflowoth HEPA filters, optimizing filter loading andrational life.

volume of air exhausted (and thus drawn into thet of the unit) by the Purifier is controlled by aue exhaust cover system. Rather than using a

rictive slotted plate to control the exhaust flow, thefier uses a cover with a series of controlled orifices. By opening or closing the holes, the certifier canisely control the volume of air exhausted by the

Product S


unit. This patent pending system allows for moreprecise control of the exhaust airflow, while allowingthe unit to be installed with minimal overheadclearance.

Safety PrecautionsThe Purifier Biohazard Cabinetshould be certified by a qualifiedcertification technician before itsinitial use. The cabinet should berecertified whenever it is relocated,serviced or at least annuallythereafter.

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Some components of the PurifierBiohazard Cabinet should only beserviced by a qualified certificationtechnician. Some internal componentsof the Purifier may becomecontaminated during operation of theunit. Only experienced personnelcompetent in the decontaminationprocedure should decontaminate thecabinet before servicing contaminatedcomponents. If you have anyquestions regarding certificationagencies, or need assistance in locatingone, contact Labconco�s ProductService Department at 800-522-7658or 816-333-8811.

Ensure that the unit is connected toelectrical service in accordance withlocal and national electrical codes.!

49ice 1-800-522-7658

Failure to do so may create a fire orelectrical hazard. Do not remove orservice any electrical componentswithout first disconnecting thePurifier from electrical service.

Avoid the use of flammable gases orsolvent in the Purifier. Care must betaken to ensure against the

50


concentration of flammable orexplosive gases or vapors. An openflame should NOT be used in thePurifier. Open flames may disruptthe airflow patterns in the cabinet,burn the HEPA filter and/or damagethe filter�s adhesive. Gases under highpressure should not be used in thePurifier cabinet, as they may disruptthe airflow patterns of the cabinet.

HEPA FILTERS ARE ONLYEFFECTIVE FOR THEENTRAPMENT OF PARTICULATE�

�

MATTER. MANIPULATIONSWHICH GENERATE GASES ORVAPORS, I.E., TOXIC CHEMICALSOR RADIONUCLIDES, MUST BEEVALUATED CAREFULLY FROMTHE STANDPOINT OF BUILDUP TODANGEROUS LEVELS, THEDECONTAMINATION OF THECABINET, AND COMPLIANCEWITH APPLICABLE REGULATIONS.

The surface of the HEPA filters arefragile and should not be touched.Care must be taken to avoidpuncturing either HEPA filter duringinstallation or normal operation. Ifyou suspect that a HEPA filter hasbeen damaged, DO NOT use thecabinet; contact a local certificationagency or Labconco at 800-821-5525or 816-333-8811 for recertificationinformation.

THE HEPA FILTERS IN THEPURIFIER CABINET WILLGRADUALLY ACCUMULATE

!


AIRBORNE PARTICULATEMATTER FROM THE ROOM ANDFROM WORK PERFORMED IN THECABINET. THE RATE OFACCUMULATION WILL DEPEND

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UPON THE CLEANLINESS OF THEROOM AIR, THE AMOUNT OF TIMETHE CABINET IS OPERATING ANDTHE NATURE OF WORK BEINGDONE IN THE CABINET. INTYPICAL INSTALLATIONS ANDUSAGE, THE HEPA FILTERS WILLLAST APPROXIMATELY FIVEYEARS OR MORE BEFOREREQUIRING REPLACEMENT.

Proper operation of the cabinetdepends largely upon the cabinet�slocation and the operator�s work!

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habits. Consult the Installation andNormal Operation sections of thismanual for further details.

AVOID DIRECT EXPOSURE OFPLASTIC OR COATED MATERIALSTO ULTRAVIOLET (UV)

51ice 1-800-522-7658

RADIATION. NEVER BYPASS THESAFETY INTERLOCK OR ATTEMPTTO WORK IN THE PURIFIERCABINET WHEN THE UV LIGHT ISON.

WHEN SURFACE DISINFECTINGTHE PURIFIER:

• AVOID SPLASHING THEDISINFECTING SOLUTION ON SKINOR CLOTHING.

• ENSURE ADEQUATEVENTILATION.

• CAREFULLY FOLLOW THEMANUFACTURER�S SAFETYINSTRUCTIONS WHEN HANDLINGDISINFECTANTS AND ALWAYSDISPOSE OF DISINFECTINGSOLUTIONS IN ACCORDANCEWITH LOCAL AND NATIONALLAWS.

52


DO NOT ALLOW DISINFECTANTSWITH HIGH CONCENTRATIONS OFFREE CHLORINE TO CONTACT THESTAINLESS STEEL COMPONENTSOF THE PURIFIER FOR A LONGPERIOD OF TIME. FREE CHLORINEWILL CORRODE STAINLESS STEELAFTER EXTENDED CONTACT.

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The electrical receptacle cover,because of its construction, may bedifficult to surface decontaminate. Inthe event of gross contamination, thecover should be removed, sterilizedand/or decontaminated as requiredand discarded. The receptacle covershould then be replaced with therepair part listed in Appendix A:Purifier Components.

Biohazard cabinets should bedecontaminated using formaldehydegas for any of the following reasons:

• Maintenance work requiring entryinto contaminated areas.

• Before HEPA filter changes.• Before performing performance tests

requiring entry into contaminatedareas.

• Before moving the cabinet.• Before changing research programs.• After the gross spill of biohazardous

material.

THE PROCEDURES FORPERFORMING A GASEOUSDECONTAMINATION ARE


THOROUGHLY OUTLINED IN THEU.S. DEPARTMENT OF HEALTH,EDUCATION AND WELFAREBOOKLET ENTITLED �FormaldehydeDecontamination of Laminar FlowBiological Safety Cabinets,� WHICH ISAVAILABLE FROM THE NATIONAL



INSTITUTES OF HEALTH, DIVISIONOF SAFETY, BETHESDA,MARYLAND 20892, OR FROMLABCONCO CORPORATION.

Your Next StepAfter you understand the theory of operation and safetyprecautions, you are ready to proceed to Chapter 5:Using Your Purifier.




CCHHAAPPTTEERR 55UUSSIINNGG YYOOUURR

PPUURRIIFFIIEERR

Operating the Sliding SashBecause of the Purifier's counterbalanced, anti-rackingsash mechanism, it will take only a few pounds of forceto move the sash up or down, and you can operate thesash smoothly with one or two hands positioned anywhere along the handle.

The sash position alarm and safety interlock systemsense the sashes position and act appropriately. YourPurifier is designed to operate with either an 8- or 10-inch sash opening. Raising the sash above its operatingheight will activate the audible and visual alarms. Theaudible alarm can be temporarily muted (forapproximately five minutes) by depressing andreleasing the alarm silence button. Closing the sashback to its operating position will reset the alarm anddefeat the muting of the alarm. The safety interlocksystem senses when the sash is closed, and will shut offthe cabinet blower (to prevent it from overheating). Theinterlock also allows the optional ultraviolet (UV) lampto operate only when the sash is closed, to protect theoperator from irradiation.


Chapter 5: Using Your Purifier

1. To start the Purifier, raise the sash until you feel thesash tactile position indicator (TPI). The TPIfunctions by increasing the resistance you feel onthe sash as it nears its proper operating position.Once in position, the TPI will 'set', requiringadditional upward force to be raised further. Thecorrect sash position can be confirmed by aligningthe handle of the sash with the indicator decallocated on the left side of the opening. The indicatordecal is shown in Figure 5-1.

Figure 5-1

Product Service 1-8


2. Turn the blower switch to the 'On' position, asshown in Figure 5-2. The audible and visual alarmswill self-test and be activated for approximatelythree seconds. The alarms will then shut off. If thealarms continue after three seconds, recheck thesash position.

The Type Bvelocity alaexhausting drops belowoccurs:

Figure 5-2

5700-522-7658

3 Purifier Cabinets are equipped with a facerm system that monitors the volume of airfrom the cabinet. If the exhaust volume the minimum requirement, the following

58


• An audible alarm sounds.• The red "Alarm" indicator light illuminates.• The cabinet blower, supplying the downflow air

in the work area, shuts off.

During an alarm, the cabinet blowershuts off to prevent air from escapingthe work area of the cabinet.

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If the alarm reactivates after fiveseconds, DO NOT continue to use thePurifier. NEVER OVERRIDE THEALARM RESET SWITCH TOCONTINUE WORKING IN THECABINET. You MUST correct theproblem in the exhaust system beforeresuming operations.

The Alarm Activates most common causes of alarm activation are:

• Mechanical failure of the external blower.• An obstruction or leak in the exhaust system.• Loading of the exhaust HEPA filter.


If the alarm sounds during use,immediately take appropriate actionto prevent contamination to you andother personnel in the area.

setting The Alarmstem

e the cause of the face velocity alarm has beenrmined and corrected, the alarm may be reset byressing the "Alarm Silence" switch.

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59ervice 1-800-522-7658

IMPORTANT: If pressing the "AlarmSilence" switch does not reset thecabinet into normal operation, or thealarm activates frequently, DO NOTcontinue to use the Purifier. NEVEROVERRIDE THE ALARM RESETMECHANISM TO CONTINUEWORKING IN THE CABINET. YouMUST correct the problem in theexhaust system before resumingoperations.

orking In Your Purifierlanning

• Thoroughly understand procedures andequipment required before beginning work.

• Arrange for minimal disruptions, such as roomtraffic or entry into the room while the cabinet isin use.

tart-up• Turn off UV light if included.• Slowly raise the sash until the TPI engages at

the proper height. Confirm the sash is at itsproper height by examining the sash indicatordecal located on the left side of the work area.

• Turn on fluorescent light and cabinet blower.• Check the return air grilles for obstructions, and

note the pressure gauge reading.• Allow the cabinet to operate unobstructed for 5

minutes.• Wash hands and arms thoroughly with

germicidal soap.• Wear a long sleeved lab coat with knit cuffs and

over-the- cuff rubber gloves. Use protectiveeyewear. Wear a protective mask if appropriate.

ipe-Down• Raise the sash to its full open position

(approximately 19 inches open). Mute the alarmby depressing the "Alarm Silence" switch.



• Wipe down the interior surfaces of the cabinetwith 70% ethanol, or a suitable disinfectant, andallow to dry.

Loading Materials and Equipment• Only load the materials required for the

procedure. Do not overload the cabinet.• Do not obstruct the front, side, or rear return air

grilles.• Large objects should not be placed close

together.• Slowly close the sash until the TPI is engaged

correctly.• After loading the cabinet, wait two to three

minutes to purge airborne contaminants fromthe work area.

Work Techniques• Keep all materials at least 4 inches inside of the

sash, and perform all contaminated operationsas far to the rear of the work area as possible.

• Segregate all clean and contaminated materialsin the work area.

• Arrange materials to minimize the movement ofcontaminated materials into clean areas.

• Keep all discarded contaminated material to therear of the work area.

• Avoid moving materials or the operator's handsand arms through the front access openingduring use.

• Avoid the use of an open flame.• Use proper aseptic technique.• Avoid using techniques or procedures that

disrupt the airflow patterns of the cabinet.• If there is a spill or splatter during use, all

objects in the cabinet should be surfacedecontaminated before removal. Thoroughlydisinfect the working area of the cabinetWHILE IT IS STILL IN OPERATION. Thiswill prevent the release of contaminants fromthe cabinet.



Final Purging• Upon completion of work, the cabinet should be

allowed to operate for two to three minutesundisturbed, to purge airborne contaminantsfrom the work area.

Unloading Materials and Equipment• Objects in contact with contaminated material

should be surface decontaminated beforeremoval from the cabinet.

• All open trays or containers should be coveredbefore being removed from the cabinet.

Wipe-Down• Wipe down the interior surfaces of the cabinet

with 70% ethanol, or a suitable disinfectant, andallow to dry.

• Periodically lift the work surface and wipedown the area beneath it.

• Inspect and clean the towel catch located at therear of the work area, beneath the work pan.

• Dispose of rubber gloves appropriately, andhave lab coat laundered properly.

• Wash hands and arms thoroughly withgermicidal soap.

Shutdown• Turn off the fluorescent light and cabinet

blower, close the sash, and turn on the UV lightif appropriate.

Safety and ComfortMany factors in our work environment determinewhether we work efficiently and in a manner thatpromotes good health and safety. By considering,acting on, and periodically reevaluating therecommendations in this section, it is possible to createa safer, more comfortable, healthful, and efficient workenvironment.

62


Working intensely or for a long timein uncomfortable or unnaturalpositions poses risks.

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anizing Your Adjustments order in which you make various adjustments tor body position and work area may vary dependinghe adjustability of your furniture.

LABCONCO OFFERS AN OPTIONALERGONOMIC CHAIR ANDADJUSTABLE FOOTREST. FOR
FURTHER INFORMATION SEEAppendix D: Purifier Accessories.
PAY PARTICULAR ATTENTION TOADJUSTING YOUR POSTURE INTHE AFTERNOON WHEN YOU MAYTEND TO GET FATIGUED.

WHEN YOU REPOSITION ANY ONEPART OF YOUR BODY, YOU MANYNEED TO ADJUST OTHER PARTSAS WELL.

Don�t sit in one fixed posture all day.

t, Knees, and Legse sure your feet can rest solidly and comfortably on

floor or footrest while sitting. Use an adjustableir that allows your feet to rest firmly on the floor, ora footrest. If you use a footrest, be sure it is wideugh to accommodate different leg positions withinr comfort zone.

REST YOUR FEET FIRMLY ON THEFLOOR OR A FOOTREST.

Don�t dangle your feet and compressyour thighs.


Product Serv


Providing Enough Leg RoomBe sure you have sufficient space under your worksurface for your knees and legs. Avoid concentratedpressure points along the underside of your thigh nearthe knee and the backside of your lower leg. Stretchyour legs and vary your leg posture throughout the day.

Leg ComfortVary your leg positions throughout the day.

Avoid placing boxes or other itemsunder the cabinet that limit your legroom. You should be able to pull your

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chair all the way up to your Purifierwithout interference.

lk up from your Purifier periodically and take briefks.

k your chair to fully support your body. Distributer weight evenly and use the entire seat and backrestupport your body. If your chair has adjustable lowk support, match the contours of the chair's backreste natural curve of your lower spine. Always make your lower back is well supported. Make sure its comfortable in the position in which you areking.

DISTRIBUTE YOUR WEIGHTEVENLY AND USE THE ENTIRESEAT AND BACKREST TO
SUPPORT YOUR BODY.
Don�t slouch forward.

63ice 1-800-522-7658

ust oftenour chair is adjustable, experiment with thestments to find numerous comfortable positions, adjust the chair frequently.

64


If you are using a chair for the firsttime, or if you share a chair withsomeone else, don�t assume the

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settings are properly set for you.

earms, Wrists, and Handsp your forearms, wrists, and hands aligned in aight, natural position. Avoid bending or anglingr wrists while working.

't Anchor Your Wristsen working, do not anchor or rest your wrists on thek surface. Resting your palms while working mayarmful because it can cause you to bend your wrists

k and can apply pressure to the undersides of yourts.

MAINTAIN A STRAIGHT, NATURALWRIST POSITION WHILEWORKING.

Don�t rest your palms on the worksurface while working.


Don�t anchor your wrists, this maycause unnecessary strain.

ulders and Elbowsust your chair height or Purifier height so that yourulders are relaxed and your elbows hangfortably at your sides.

axember to relax, particularly in areas where muscle

ion often builds, such as your shoulders.

srking at your Purifier for long periods can be aally demanding task and may cause your eyes to

ome irritated and fatigued. Therefore, you should special attention to vision care, including the

owing recommendations:

Product S


Resting Your EyesGive your eyes frequent breaks. Periodically look awayfrom the work area and focus at a distant point. Thismay also be a good time to stretch, breathe deeply, andrelax.

Cleaning Your Sash and GlassesKeep your sash and glasses clean.

Having Your Eyes ExaminedTo be sure that your vision is adequately corrected,have your eyes examined regularly by a vision carespecialist. If you wear bifocals or trifocals, you mayfind monofocal glasses more comfortable for cabinetuse.

Eye comfortWhile working in your Purifier, and also while restingyour eyes, remember to blink. This helps keep youreyes naturally protected and lubricated, and helpsprevent dryness, a common source of discomfort.

GIVE YOUR EYES FREQUENTRESTS BY FOCUSING THEM ON ADISTANT POINT.

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rranging Your Work Arealiminating Glare and Reflections on the Sashake the time to eliminate glare and reflections. Toontrol daylight, use blinds, shades, or drapes, or tryther glare-reducing measures. Use indirect or reducedghting to avoid bright spots on the sash. If glare is aroblem, consider these actions:

• Turn off or reduce ceiling lights and use tasklighting (one or more adjustable lamps) toilluminate your work.

Avoid compromising your posture tocompensate for glare or reflections.!

65ice 1-800-522-7658

66


Positioning Work MaterialsPosition work materials directly in front of you to avoidtwisting your neck and torso. This makes it possible towork with your shoulders relaxed and your upper armshanging freely at your sides.

Minimizing Your ReachArrange your frequently used materials to minimize thedistance you reach for them.

Hand ManipulationNotice how you use your fingers and hands whileworking. Avoid keeping any unnecessary tension inyour thumbs and fingers. Observe whether you have aheavy touch or a light touch while working.

Reaching for MaterialsTo reach material that is not near your hands, moveyour whole arm; avoid stretching your fingers andangling your wrists.

RELAX YOUR THUMBS ANDFINGERS WHILE WORKING;NOTICE AND RELEASE EXCESS

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Don't work with unnecessary tensionin your thumbs and fingers.

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ist Position your whole arm and shoulder to move objects, not your wrist. Don't rest or anchor your wrist whileing items; keep your wrist, arm, and shoulder freeove.

ning Forearms, Wrists, and Handsen working in the Purifier, keep your forearms,ts, and hands comfortably aligned with each other straight, natural line. Avoid bending or anglingr wrists.

ing Breaks and Varying Your Tasksnoted earlier, your furniture placement, labipment, and lighting are only a few of the factors

Product S


that determine comfort. Your work habits are also veryimportant. Remember the following:

• Take breaks.• When you work at your Purifier for long

periods, take short breaks at least once per hourand preferably more often. You may find thatfrequent, short breaks will benefit you morethan fewer, longer breaks.

• If you find that you forget to take breaks, use atimer.

• During your breaks, stand up and stretch,especially any muscles and joints you may haveheld in an extended static posture whileworking.

Vary Your TasksExamine your work habits and the types of tasks youperform. Break up the routine and try to vary your tasksduring the day. By doing so, you may avoid sitting inone position or performing the same activitiescontinuously for several hours using your hands, arms,shoulders, neck, or back.

Reduce Sources of StressTake an inventory of things at work that are stressful toyou. If you perceive that your physical or psychologicalhealth is being affected, take time to evaluate whatchanges you can make to reduce or eliminate thesources of stress.

BREATHE FRESH AIR DEEPLY ANDREGULARLY. THE INTENSEMENTAL CONCENTRATION THAT

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67ervice 1-800-522-7658

MAY ACCOMPANY WORK IN ABIOSAFETY CABINET MAY TENDTO CAUSE BREATH-HOLDING ORSHALLOW BREATHING.

elf-Checko increase your comfort and reduce potential safetysks, use this checklist to help you evaluate your workosture and habits.



Seated Position• Have you found a range of seated postures that

are most comfortable for you?• Are you changing postures within your "comfort

zone" throughout the day, especially in theafternoon?

• Are your feet firmly planted?• Are the undersides of your thighs near your

knees free of pressure?• Are the backs of your lower legs free of

pressure? Is there sufficient space under yourcabinet for your knees and legs?

• Is your lower back supported?

Shoulders, Arms, Wrists, and Hands• Are your shoulders relaxed?• Are your hands, wrists, and forearms aligned in

a straight, natural position?• Are your elbows in a relaxed position near your

body?• Are items you use frequently easy to reach?

Eyes• Do you rest your eyes frequently by focusing on

a distant point?• Do you get your eyes examined regularly by a

vision care specialist?• Do you blink enough?• If you wear bifocals or trifocals, do you avoid

tilting your head back to see your work?

Working Style• Are you training your fingers to relax when they

become tense?• Do you use your whole arm to reach for objects

not located near your fingers?• Is your work positioned directly in front of you?• Are your wrists straight and your shoulders

relaxed?• Have you eliminated glare and bright reflections

on the sash, without compromising yourposture?



General Prevention• Do you take breaks and walk around briefly,

preferably once per hour?• Do you exercise regularly?• Periodically, do you take inventory of the stress

in your life and change what is within yourcontrol to change?

Your Next StepAfter you understand how to operate and work in thePurifier, you are ready to proceed to Chapter 6:Maintaining Your Purifier.



Product Serv

CCHHAAPPTTEERR 66MMAAIINNTTAAIINNIINNGG YYOOUURR

PPUURRIIFFIIEERR

Now that you have an understanding of how to work inthe Purifier, we will review the suggested maintenanceschedule and the common service operations necessaryto maintain your Purifier for peak performance.

Many of the service operations shouldbe performed only by trained andexperienced certification technicians

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after the cabinet has been properlydecontaminated. DO NOT attempt toperform these operations if you arenot properly trained. The serviceoperations that require qualifiedcertifiers are preceded by the wrenchicon.

utine Maintenancehedule

ekly• Using 70% ethanol, or a suitable disinfectant,

surface disinfect the inside of the cabinet, andthe work surface.

72

Chapter 6: Maintaining Your Purifier

• Using an appropriate glass cleaner, clean thesash and the surface of the UV lamp, if soequipped.

• Operate the cabinet blower, noting the pressurereading in an operational log.

Monthly (or more often as required)• Using a damp cloth, clean the exterior surfaces

of the cabinet, particularly the front and top ofthe cabinet, to remove any accumulated dust.

• Disinfect and lift the work surface. Surfacedisinfect the lower plenum with a solution of70% ethanol, or a suitable disinfectant. Checkthe towel catch for retained materials.

• Check all service valves, if so equipped, forproper operation.

• All weekly activities.

Annually• Have the cabinet recertified by a qualified

certification technician.• Replace the UV lamp, if so equipped.• All monthly activities.

Biannually• Replace the fluorescent lamp.

Service OperationsWork Surface Removal:

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The work surface of the cabinet mustbe thoroughly decontaminated beforeremoving it.

. Loosen and remove the thumbscrews located at thefront of the work surface, as shown in Figure 6-1.

. Only loosen the thumbscrews located at the rear ofthe work surface.

. Lift the front edge of the work surface up and pull itout of the cabinet work area.

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Towel Catch Removal:

Although not normally required, the towel catch can beremoved for cleaning, inspection, etc.

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The work surface of the cabinet andthe towel catch must be thoroughlydecontaminated before removingeither.

. Remove the work surface as described above.

. Loosen and remove the thumbscrews located at therear of the work surface.

. Surface decontaminate the towel catch beforeremoving it.

Figure 6-1



Front Panel Removal:

1. Locate and remove the two Phillips screws thatsecure the front panel as shown in Figure 6-2.

2. Swing the bottom of the dress panel out to clear thefluorescent light and then lift the front dress panelstraight up and away from the cabinet.

Figure 6-2

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Changing the Fluorescent Lamp:

1. Unplug the cabinet.2. Remove the front dress panel as noted in Figure 6-2.3. Remove the fluorescent lamp by rotating it 90

degrees and pulling it straight up and out of itssockets.

4. Remove the fluorescent lamp by pushing it to theleft and swinging it out of the right lamp socket.

5. Install the new lamp by reversing the removalprocedure.

Changing the Optional UV Lamp:

FOR OPTIMUM PERFORMANCE,THE UV LAMP SHOULD BECHANGED ON AN ANNUAL BASIS.

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The UV lamp and the work area of thecabinet must be thoroughlydecontaminated before removing thelamp.

. Start the cabinet and let it operate for 5 minutes.

. Thoroughly surface decontaminate the UV lampand the work area of the cabinet.

. Unplug the cabinet.

. Raise the sash to its full open position.

. Remove the UV lamp by rotating it 90 degrees andlifting it straight up and out of its sockets.

. Remove the UV lamp by pushing it to the left andswinging it out of the right lamp socket.

. Install new lamp by reversing the removalprocedure.

esetting a Circuit Breaker:

o reset any of the circuit breakers located on the frontf the electronics module, depress the white button until sets.

The service operations listed in therest of this chapter should only beperformed by a qualified certifier.

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Downflow VelocitiesThe average downflow velocity for all model Purifiersshould be set at 55 ±5 FPM.

Downflow Velocity GridPatternsThe downflow velocity test grid for all model Purifiersis listed on the data plate on the upper right side of thesash when it is completely lowered. If the decal ismissing or obliterated, contact Labconco's ProductService Department at 1-800-522-7658 for furtherdetails.

Inflow Velocity Calculation -Primary MethodThe average inflow velocity for all Purifiers is 105 ±5FPM, and should be determined by converting theinflow volume to the average inflow velocity. Theinflow volume is measured directly by sealing a directinflow measuring flowmeter to the face of the unit.When corrected for local conditions, dividing theinflow volume by the opening area will yield theaverage inflow velocity.

PurifierModel

SashHeight(inches)

Avg. InflowVelocity(FPM)

WorkOpeningArea (sq. ft.)

InflowVolume(CFM)

36204 10 105 ±5 2.53 26636205 8 105 ±5 2.03 21336208 10 105 ±5 3.37 35436209 8 105 ±5 2.69 28336212 10 105 ±5 5.03 52936213 8 105 ±5 4.03 423

Table 6-1



Inflow Velocity Calculation -Secondary MethodIf the primary method cannot be performed, thesecondary inflow calculation method should beperformed. The secondary method uses a hot wirethermal anemometer. In this method, the sash islowered until the open area of the sash equals onesquare foot, and a series of velocity readings are takenat the front of the unit.

1. For each model Purifier, the sash height should beadjusted and measured such that a vertical line fromthe lowest surface of the sash handle to the frontgrille should equal the following height:

Purifier Models Sash height opening36204, 36205 3.95 in (100 mm)36208, 36209 2.97 in (75 mm)36212, 36213 1.99 in (51 mm)

2. Start the Purifier, and let it operate for at least 5minutes.

3. Establish the necessary correction factor to thethermal anemometer to ensure compliance with itsperformance in a calibrated wind tunnel. This datashould be available from the calibrator of thethermoanemometer.

4. Calculate the manufacturer�s recommendedcorrection factor(s) to correct for local conditions oftemperature, humidity, barometric pressure,altitude, etc.

5. Measure a number of test points, 4 inches from eachside wall, and at intervals of the following distancefor each of these models:

Table 6-2


ModelNumbers

Distance Between eachTest Point (inches)

Total Number ofTest Points

36204, 36205 4.07 836208, 36209 4.05 1136212, 36213 4.03 17

6. Take a series of inflow velocities by placing thesensor of the thermal anemometer at the test points,in the center of the opening, leaning the body of theprobe against the front of the grille.

7. Apply the wind tunnel correction and localcondition correction factors to the thermalanemometer readings.

8. Multiply the individual readings by the followingPurifier correction factor:

Model Numbers Purifier Correction Factors(CFM/FPM)

36204* 1.0836205* 1.0636208 1.1336209 1.1236212 1.3036213 1.24

Average the corrected readings.

9. The resulting value is the inflow volume in cubicfeet per minute (CFM). In order to convert thisvalue to the average inflow velocity of feet perminute (FPM), divide the volume by the sash openarea given in Table 6-5 below.

Model Numbers Sash Open Area (square feet)36204 2.5336205 2.0336208 3.3736209 2.6936212 5.0336213 4.03

Table 6-3

Table 6-4

Table 6-5

*On models with serial #99xx, 0001 through 0007, the correction factor for model 36204 is 1.13 andfor 36205 is 1.11.




10. The resulting value will be the calculated averageinflow velocity in FPM.

Electronics Module AccessThe electronics module assemblyshould be serviced by a qualifiedcertification technician.

The electronics module houses the speed control, thelamp ballasts, the circuit breakers, as well as the motorcapacitor.

1. Unplug the cabinet.2. Remove the two 7/16" acorn nuts that secure the

electronics module to the top of the cabinet, shownin Figure 6-3.

3. Pivot the module onto its back surface to access theelectronic components within.

Figure 6-3

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Measuring Line and MotorVoltage

Line and motor voltage contact pointsare high voltage connections. Usecaution when measuring eithervoltage.

A VOLTAGE CONNECTOR CAN BEFABRICATED FROMCOMMERCIALLY AVAILABLE

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ELECTRONICS HARDWARE, ORONE IS AVAILABLE TO QUALIFIEDCERTIFIERS FROM LABCONCOCORPORATION. CONTACTLABCONCO'S PRODUCT SERVICEDEPARTMENT FOR FURTHERDETAILS.

. Remove the access panel located on the front of theelectronics module by removing the right handPhillips screw, and loosening the left hand screw asshown in Figure 6-4. Pivot the panel out of the way.

. Locate the voltage test points on the electricalpanel. The test points are identified by Figure 6-5,shown below.

. Using a voltmeter and probes, measure the desiredvoltage by connecting the voltmeter probes to theappropriate points.

Figure 6-4

Product Serv


Speed Control AdjustmentThe Purifier blower speed should onlybe adjusted by a qualified certificationtechnician. Adjustment of the blowerspeed will affect the containmentefficiency of the unit.

The Purifier�s blower speed isadjusted and its setpoint is maintainedby a solid state electronic circuit.

1.

2.

3.

Figure 6-5

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There is no potentiometer as ontraditional biosafety cabinets. Theblower speed setting is adjusted andprotected by an electronic interlockthat must be released to reset themotor voltage.

Remove the access panel located on the front of theelectronics module by removing the right handPhillips screw, and loosening the left hand screw asshown in Figure 6-4. Pivot the panel out of the way.Locate the voltage test points on the electricalpanel. The test points are identified by Figure 6-5,shown above.Using a voltmeter and probes, measure the motorvoltage by connecting the voltmeter probes to theappropriate points.

82


4. Shut the Purifier off.5. While holding down the "alarm silence" button, turn

the "System Power" switch on.6. The blower will start, and the audio and visual

alarm will operate in an alternating mode. Theblower speed can now be adjusted.

7. The blower speed can be increased by pressing thebutton on the right; pressing the button on the leftdecreases the blower speed as shown in Figure 6-4.

8. Once the proper blower speed has been establishedby inflow and downflow testing, the new blowervoltage can be programmed into the speedcontroller by depressing the "Alarm Silence" buttonagain. The audio alarm will sound a single longtone, and then shut off, indicating the new voltage isproperly programmed.

IF YOU WISH TO CANCEL THENEW BLOWER VOLTAGE SETTING,SIMPLY TURN THE �SYSTEM

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POWER� SWITCH OFF BEFOREDEPRESSING THE �ALARMSILENCE� BUTTON. THIS WILLCANCEL THE NEW SETTING, ANDRETURN THE SPEED CONTROLLERTO ITS PREVIOUS VALUE.

xhaust Control Systemdjustment

THE PURIFIER IS EQUIPPED WITHA UNIQUE EXHAUST CONTROLSYSTEM, UNLIKE ANY OTHER
BIOSAFETY CABINET. IF YOUHAVE ANY QUESTIONS ABOUT ITSOPERATION OR ADJUSTMENT,CONTACT LABCONCO'S PRODUCTSERVICE DEPARTMENT FORFURTHER DETAILS.
Adjusting the exhaust control systemwill have an effect on the air velocitiesand the efficiency of the cabinet. The!


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exhaust control system should only beadjusted by a qualified certificationtechnician as part of therecertification procedure.

IF YOU NEED ADDITIONALEXHAUST CONTROL SYSTEMPLUGS, CONTACT LABCONCO'S

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PRODUCT SERVICE DEPARTMENT.

ome early 3-foot wide Purifiers with serial numberseginning in 99xx or 0001 through 0007, werequipped with a single piece, orange exhaust cover. Asown in Figure 6-6.

. Locate the exhaust filter cover located on the top ofthe cabinet.

. To increase the flow of exhaust air, remove one ormore of the exhaust hole plugs. The plugs "lock"into the cover. To remove the plug, reach throughan adjacent hole, and depress the tabs that securethe plug into the cover.

. To decrease the exhaust flow, insert one or moreplugs back into the vacant holes in the exhaustcover. Additional plugs are stored in the parts bagshipped with the cabinet.

. Reestablish the inflow, making sure it meets thespecification mentioned in Table 6-1.

Figure 6-6

3'

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Later 3-foot units, as well as all 4-foot and 6-foot widePurifiers utilize a two piece exhaust cover system.

1. Remove the four nuts that secure the orange exhaustdiffuser cover from the top of the Purifier.

Do not remove or attempt to adjustthe angled deflector blades on theprimary exhaust cover.

2.

3.

4.

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To increase the exhaust flow, remove blockingstrip(s) from the primary exhaust cover, install themin the storage areas at the front or rear of the cover.Reinstall the exhaust diffuser cover.To decrease the exhaust flow, remove surplusexhaust cover strip(s) from the front or back storagearea, and install them over an open row of holes.Reinstall the exhaust diffuser cover.Reestablish the inflow, making sure it meets thespecifications in the chapter.

Figure 6-7

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Use of the Access Tube ForDOP Testing

THE PURIFIER UTILIZES A UNIQUEACCESS TUBE FOR SAMPLING THE

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UPSTREAM CONCENTRATION OFDOP. IF AFTER READING THESEINSTRUCTIONS, YOU HAVEFURTHER QUESTIONS, PLEASECONTACT LABCONCO'S PRODUCTSERVICE DEPARTMENT.
The access tube containscontaminated air under positivepressure. DO NOT access this lineuntil the cabinet has beendecontaminated. DOP scanning of theHEPA filters should only beperformed by a qualified certificationtechnician as part of therecertification procedure.

. Remove the work surface as shown in Figure 6-1.

. Locate the access tube on left side of the rearplenum wall, behind the towel catch.

. Pull the tube toward the front of the unit. Removethe access tube's cover.

. Using an appropriate photometer, connect the unit'supstream line to the access tube.

. After completing the DOP test of the filters, replacethe access tube cover, ensuring that it is sealedproperly.

. Replace the access tube, and the work surface.

EPA Filter Replacement

85ervice 1-800-522-7658

The cabinet must be properlydecontaminated before servicing thesupply HEPA filter. The HEPA filtersshould only be serviced by a qualified



certification technician. Followingreplacement of a HEPA filter, thecabinet MUST be recertified by aqualified certification technician.

1. Unplug the cabinet.2. Remove the front dress panel as shown in Figure

6-2.3. Remove the sash pocket by loosening the two

Phillips screws on the left side of the pocket.Support the sash pocket while removing the twoscrews on the right side of the sash pocket. Swingthe sash pocket out and away from the unit asshown in figure 6-8.

Figure 6-8

Product Serv


4. Loosen and remove all of the Phillips screws on theblower chamber cover. Gently rock the cover, slideit to the left or right, and swing it out and awayfrom the unit.

5. Using a 1/2 inch wrench, loosen the four lockingbolts that contact each corner of the plenum box atleast 1/2 inch, as shown in Figure 6-8.

6. Loosen the four exhaust filter sealing bolts locatedat each corner of the exhaust filter tray, as shown inFigure 6-9 at least 1/2 inch. This releases theexhaust HEPA filter from the cabinet.

To Remove the Exhaust HEPA Filter:

The exhaust HEPA filter is awkwardto handle and heavy. Use appropriatelifting techniques to remove and

1.

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Figure 6-9

87ice 1-800-522-7658

handle it. Use two people to removethe filter if possible.

Slide the exhaust HEPA filter out of the filter pan.

88


To Remove the Supply HEPA Filter:

The supply HEPA filter is awkward tohandle and heavy. Use appropriatelifting techniques to remove and

1.

2. 3.

To

1.

2.

3.

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handle it. Use two people to removethe filter if possible.

With the exhaust filter removed, use a 1/2-inchsocket or wrench to tighten the three plenum liftbolts located as noted in Figure 6-9. This will liftthe plenum box off the supply HEPA filter.Slide the supply HEPA filter straight out the unit.With the HEPA filters removed, inspect the filtershelf and the exhaust filter tray for damage.

Install a Supply HEPA Filter:

Install a new supply HEPA filter by pushing itstraight into the cabinet, ensuring that it is correctlyoriented, and that the filter is centered on the filtershelf.Use a 1/2-inch socket or wrench to sequentiallyloosen the three plenum lift bolts located as noted inFigure 6-9. When properly positioned, the plenumbox will be aligned with the supply HEPA filterframe, and there will be an approximately 1/4 inchgap between the bottom of the plenum lift bolt headand the top of the lift bracket. This will allow theplenum box to properly seat as the filter gasket iscompressed.Using a 1/2-inch wrench, tighten the four lockingbolts located at each corner of the plenum box, asshown in Figure 6-9 until the supply HEPA filtergasket is compressed to approximately 50% of itsthickness.

The locking bolts should only betightened enough to ensure a properseal of the supply HEPA filter. At


maximum tightness, the supply filter'sgasket should be compressed to 50%of its original thickness. NEVERtighten the locking bolts beyond thissetting.

Product Serv


To Install an Exhaust HEPA Filter:

1. Slide the new exhaust HEPA filter all the way intothe pan.

2. Sequentially tighten the four exhaust filter sealingbolts located at each corner of the exhaust filtertray, as shown in Figure 6-9 until the exhaust filtergasket is compressed approximately 50%.

The exhaust filter sealing bolts shouldonly be tightened enough to ensure aproper seal of the exhaust HEPA

3.

4. 5. 6.

Man

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filter. At maximum tightness, theexhaust filter's gasket should becompressed to 50% of its originalthickness. NEVER tighten the boltsbeyond this setting.

Install the blower chamber cover, uniformlytightening the screws on the cover until the gaskethas formed a proper seal.Install the sash pocket.Install the front dress panel.Plug in the cabinet and have it recertified beforeuse.

otor/Blower Maintenanced Replacement

The cabinet must be properlydecontaminated before servicing the motor/blower.

The motor/blower should be servicedby a qualified certification technician.

The cabinet blower motor's bearingsare sealed and require no lubrication.DO NOT attempt to lubricate them.

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90


To replace the Motor/Blower:

1. Unplug the cabinet.2. Remove the front dress panel as shown in Figure

6-23. Remove the sash pocket as shown in Figure 6-8.4. Loosen and remove all of the Phillips screws on the

blower chamber cover. Gently rock the cover, slideit to the left or right, and swing it out and awayfrom the unit.

5. Using a screwdriver, disconnect the motor groundwire from the blower housing.

6. Disconnect the motor leads from the wiring harnessby pulling apart each of the 4 connectors.

7. Using a 1/2-inch wrench, remove the two upper andone lower blower mounting bolts, as shown inFigure 6-10.

The motor/blower assembly is heavy.Handle with care.

8.

9.

10.

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While supporting the blower assembly, pull theassembly straight out the plenum.To replace the motor/blower assembly, reverse theabove procedure.Plug in the cabinet and have it recertified beforeuse.

Figure 6-10

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Diffuser Removal

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The work area of the cabinet must bethoroughly disinfected beforeremoving the diffuser. Beforeremoving the diffuser, note itsorientation. It can only be installedone way. Bending or distorting thediffuser may affect the airflow in thecabinet. Use care when handling it.When properly installed, the rear edgeof the diffuser should rest on thediffuser support brackets located onthe rear wall of the work area asshown in Figure 6-11.

. Shut off the Purifier and raise the sash to the fullopen position. If the diffuser extends to the front ofthe filter shelf, remove the sash pocket, as describedin Figure 6-8.

. Remove the fasteners located in the front or lowerfront edge of the supply HEPA filter shelf.

. Carefully lower the diffuser and the restrictor platelocated above it. Remove them, by tilting the frontedge down.

. Install the diffuser and restrictor plate by reversingthe above steps.

Figure 6-11

92


StorageIf the Purifier cabinet is to be left unused for more thanone month, the unit should be prepared for storage.

The cabinet should not be stored inareas of excess humidity ortemperature extremes. If the cabinet

1.

2. 3.

De

Thegasecabsurfpos

To

1.

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is moved during storage, it must berecertified before use.

Close the sash completely and seal the bottom edgeand the exhaust outlet with plastic sheeting.Unplug the unit.Ensure that the cabinet will not be moved ordisturbed while in storage.

contaminationGaseous decontamination should onlybe performed by trained personnel.

unique sash pocket of the Purifier allows for theous decontamination of the entire interior of the

inet, as well as the inside of the sash, and the outsideace of the blower cover, an area which maysibly become contaminated during operation.

seal the Purifier follow these steps:

Remove the front dress panel as shown in Figure6-2. Place decontamination equipment inside thework area of the Purifier. Remove the sash stopsfrom the lower front edge of the unit. Close thesash until it covers the lower edge of the unit.

The outlets on the interior of thePurifier are energized as long as thePurifier is plugged in. Unplug the


Purifier before decontaminationequipment is plugged into theseoutlets.



2. Using a flexible sealant, plug the area around thesash cables where they exit the top of the sashpocket.

3. Use duct tape to seal the outside edge of the sashpocket to the side panels.

4. Seal the upper and lower edges of the sash pocketusing duct tape.

5. Seal the sides of the sash to the corner posts, and thesash handle to the lower front edge of the unit.

6. Perform the decontamination as described in NSFStandard 49, Annex G.

Your Next StepAfter you understand the maintenance procedures, youare ready to proceed to Chapter 7: Modifying YourBiosafety Cabinet.



Product S

CCHHAAPPTTEERR 77MMOODDIIFFYYIINNGG YYOOUURR

BBIIOOSSAAFFEETTYY CCAABBIINNEETT

There are several ways to modify your Purifier for yourindividual requirements. These include the addition ofa UV lamp, service fixtures or an intravenous (IV) bar.

Installing a UV LampAll Labconco Purifier Delta Series Safety Cabinets arepre-wired for the installation of a UV lamp.

3- and 4-foot units require the installation of a UV lampkit, consisting of the UV lamp, its ballast, and fasteners.The kit is part number 37450-00 for 115 Volt models,37450-01 for 230 Volt units.

The UV lamp kit should be installedby a qualified electrician or certifier.

3' 4'

95ervice 1-800-522-7658

Completely surface decontaminate allinterior work surfaces of the Purifierbefore beginning the installation of theUV lamp.


Chapter 7: Modifying Your Biosafety Cabinet

To install the UV lamp kit do the following:

1. Unplug the unit.2. Using a 7/16-inch wrench or socket, remove the two

acorn nuts that secure the electronics module to thetop of the Purifier as shown in Figure 6-3.

3. Tilt the electronics module back to access theunderside of the module.

4. Locate the fluorescent lamp ballast in the module.The UV lamp ballast mounts next to the fluorescentballast.

5. Using the Phillips screws and locking nuts, securethe UV ballast to the enclosure as shown in Figure7-1.

6. Wire the UV ballast into the wiring harness,following the wiring diagram located in Figure 7-1.

7. Raise the sash, and install the UV lamp by rotatingthe lamp pins into the sockets.

8. Lower the sash to fully closed position, plug in theunit, and turn the system power switch to the "UV"position. The UV lamp will illuminate.

3' 4'



Figure 7-1

98


6-foot units only require the installation of the UVlamp. The lamp is part number 12713-00.

Completely surface decontaminate allinterior work surfaces of the Purifierbefore beginning the installation of theUV lamp.

To install the UV lamp kit do the following:

1. Unplug the unit, and raise the sash to the full openposition.

2. Install the UV lamp by pushing it into the leftsocket, and compress the socket, swinging it intothe right lamp socket.

3. Lower the sash to fully closed position, plug in theunit, and turn the system power switch to the "UV"position. The UV lamp will illuminate.

Installing Additional ServiceFixtures

AfoT0

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Completely surface decontaminate allinterior work surfaces of the Purifierbefore beginning the installation of theservice valve.

dditional service fixtures can be installed in any of theur available service fixture holes in both side walls.he service fixture kit is Labconco part number 36933-0. To install the service fixture:

. Decide into which of the available locations youwant to install the service fixture.

. Remove the side panel on that side of the unit asshown in Figure 3-2.

. Remove as much of the silicone sealant as possiblefrom around the outside of the service fixture holeplug.

. Placing a block of wood or plastic against theoutside edge of the plug, and using a small hammer,gently tap the plug out of the hole.

Product Serv


Do not use excessive force inattempting to remove the hole plug.Damage to the side wall of the cabinet

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could occur.

Carefully remove any remaining sealant from bothsides of the service fixture hole.Using the Teflon tape supplied, wrap two to threeturns of tape on the male pipe thread of the valve,and the tube fitting.Put the threaded end of the fixture through the hole,orienting the valve as shown in Figure 3-6. Usingthe silicone sealant, apply a bead of silicone on theoutside surface of the cabinet side, around the valvebody. This will provide a pressure tight seal aroundthe valve body. Place the two washers on the valvethread, then install the coupler. Holding the valvebody in position, use a wrench or pliers to threadthe coupling on until the valve is captured on theside panel.Thread the tubing connector onto the coupling untilit can no longer be turned by hand. Holding thecoupling secure with a wrench or pliers, turn thetube fitting approximately one more revolution,using pliers or a wrench. Orient the tube fitting suchthat it is easy to access it with the incoming servicetube.Connect the service tube to the tube fitting bypressing the tube all the way into the fitting, turningthe tubing nut until hand tight, and thenapproximately one more turn using pliers or awrench.

Make sure the service tubing does notobstruct the wiring or sash weights orcables.

99ice 1-800-522-7658

Make sure the service fitting in the Purifier is in theoff position before pressurizing the service line.Once pressurized, check the valve body, thecoupling, and the tube fitting for leaks. Tighten anyleaking joints.Reattach the side panel.

100


12. The valve is shipped with a white plastic index disc.This disc can be replaced with the orange or yellowdisc supplied by unscrewing it and replacing it withthe new one.

Installing the IV Bar

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Completely surface decontaminate allinterior work surfaces of the Purifierbefore beginning the installation of theIV bar.

. Shut off the Purifier and raise the sash to the fullyopen position.

. Remove the four black protective caps on the sidewalls and discard them.

. Mount one of the IV brackets on the two studs oneither side wall. Orient the bracket so that the centersection of the bracket is offset away from the side ofthe Purifier side wall. Secure the bracket using theflat washers, lock washers, and acorn nuts as shownin Figure 7-2.

. Slide the other bracket onto the IV bar, and install itto the side wall in the same position as the bracketon the other side wall, as shown in Figure 7-2.

. Secure the end brackets by tightening all of thefasteners.

Figure 7-2



1. Shut off the Purifier and raise the sash to the fullyopen position.

2. Remove the four black protective caps on the sidewalls and discard them.

3. Mount one of the IV brackets on the two studs oneither side wall. Orient the bracket so that the centersection of the bracket is offset away from the side ofthe Purifier side wall. Secure the bracket using theflat washers, lock washers, and acorn nuts as shownin Figure 7-2.

4. Insert the center support brace on the IV Bar. Slidethe other bracket onto the bar and install it to theside wall in the same position as the bracket on theother wall, as shown in Figure 7-3.

5. Secure the center support brace and the end bracketsby tightening all of the fasteners.

Your Next StepAfter you understand the maintenance procedures, youare ready to proceed to Chapter 8: Troubleshooting.

6'

Figure 7-3




CCHHAAPPTTEERR 88TTRROOUUBBLLEESSHHOOOOTTIINNGG

Refer to the following table if your Purifier fails tooperate properly. If the suggested corrective actions donot solve your problem, contact Labconco foradditional assistance.

PROBLEM CAUSE CORRECTIVE ACTION

Cabinet blower andlights won�t turn on

Unit not plugged intooutlet

Plug the Purifier into appropriateelectrical service.

Check connection to control box on topof unit.

Circuit breakerstripped

Reset circuit breakers.

Blower won�t turnon but lights work

Sash closed Raise sash.

Blower wiring isdisconnected

Inspect blower wiring.

Blower motor isdefective

Replace blower motor.

Cabinet blowerturns on but lightsdon�t work

Lamp not installedcorrectly

Inspect lamp installation.

Lamp is defective Replace lamp.

Lamp circuit breakertripped

Reset the lamp circuit breaker.


Chapter 8: Troubleshooting

PROBLEM CAUSE CORRECTIVE ACTION

Cabinet blowerturns on but lightsdon�t work (cont.)

Lamp wiring isdisconnected

Inspect lamp wiring.

Defective lampballasts

Replace lamp ballasts.

Slight increase inpressure reading

HEPA filter loading The pressure reading will steadilyincrease as the unit is used.

Blockage of thereturn air slots orgrille

Check all return air slots and grilles toensure that they are not blocked orrestricted.

Blockage of theexhaust outlet

Ensure that the exhaust outlet is notblocked or restricted.

Blockage orrestriction under thework surface

Ensure that the plenum beneath thework surface is unobstructed.

Contamination ofwork in the cabinet

Improper techniqueor procedure for thebiohazard cabinet

See �Use of the Cabinet� section in themanual.

Restriction of thereturn air slots orgrille � blockage ofthe exhaust outlet

Ensure that all return air slots, grillesand the exhaust outlet are unobstructed.

External factors aredisrupting the cabinetairflow patterns oracting as a source ofcontamination

See �Installation� section of thismanual.

Cabinet is out ofadjustment/HEPAfilter(s) are defective

Have cabinet recertified.


AAPPPPEENNDDIIXX AAPPUURRIIFFIIEERR

CCOOMMPPOONNEENNTTSS

Illustration A-1 indicates the location of the followingservice parts:

Item Quantity Part No. Description1 1 3734000 Side Panel, R.H.2 1 3734001 Side Panel, L.H.3 1 3739500 Exhaust HEPA Filter 3-foot

3A 1 3739501 Exhaust HEPA Filter 4-foot3B 1 3739502 Exhaust HEPA Filter 6-foot4 1 3731100 Motor/Blower Assembly, 115 VAC 3-foot

4A 1 3731101 Motor/Blower Assembly, 115 VAC 4-foot4B 1 3731102 Motor/Blower Assembly, 115 VAC 6-foot4C 1 3757100 Motor/Blower Assembly, 100 VAC 3-foot4D 1 3757101 Motor/Blower Assembly, 100 VAC 4-foot4E 1 3757102 Motor/Blower Assembly, 100 VAC 6-foot4F 1 3751900 Motor/Blower Assembly, 230 VAC 3-foot4G 1 3751901 Motor/Blower Assembly, 230 VAC 4-foot4H 1 3751902 Motor/Blower Assembly, 230 VAC 6-foot5 1 1211200 Motor, 115 VAC 3- and 4-foot

5A 1 1211200 Motor, 115 VAC 6-foot5B 1 1210503 Motor, 100 VAC 3- and 4-foot5C 1 1210501 Motor, 100 VAC 6-foot5D 1 1210502 Motor, 230 VAC 3- and 4-foot5E 1 1210500 Motor, 230 VAC 6-foot6 1 3739400 Supply HEPA Filter 3-foot

6A 1 3739401 Supply HEPA Filter 4-foot6B 1 3739402 Supply HEPA Filter 6-foot7 1 9721901 Lamp, Fluorescent, 3-foot

7A 1 9721902 Lamp, Fluorescent, 4-foot7B 1 1278400 Lamp, Fluorescent, 6-foot8 1 1271300 Lamp, UV, 3- and 4-foot (not shown)

8A 1 1231900 Lamp, UV, 6-foot (not shown)9 2 1287900 Receptacle Cover

Purifier Replacement Parts


Appendix A: Purifier Components

A-1


AAPPPPEENNDDIIXX BBDDIIMMEENNSSIIOONNSS

Canopy ConnectionB-1


Appendix B: Biosafety Cabinet Dimensions

Type B3 ConnectionB-2


AAPPPPEENNDDIIXX CCBBIIOOSSAAFFEETTYY CCAABBIINNEETT

SSPPEECCIIFFIICCAATTIIOONNSS

Cabinet Model Electrical Requirements36204-00 to �09 & 36205-00 to �09 115 VAC � 60 Hz, 1 Phase � 12 Amps36208-00 to �09 & 36209-00 to �09 115 VAC � 60 Hz, 1 Phase � 12 Amps36212-00 to �09 & 36213-00 to �09 115 VAC � 60 Hz, 1 Phase � 16 Amps

Cabinet Model Electrical Requirements36205-10 to �19 100 VAC � 50/60 Hz, 1 Phase � 12 Amps36209-10 to �19 100 VAC � 50/60 Hz, 1 Phase � 12 Amps36213-10 to �19 100 VAC � 50/60 Hz, 1 Phase � 16 Amps

Cabinet Model Electrical Requirements36205-20 to �29 230 VAC � 50/60 Hz, 1 Phase � 7 Amps36209-20 to �29 230 VAC � 50/60 Hz, 1 Phase � 7 Amps36213-20 to �29 230 VAC � 50/60 Hz, 1 Phase � 8 Amps

Cabinet Model Electrical Requirements36204-00 to �09 & 36205-00 to �0936208-00 to �09 & 36209-00 to �0936212-00 to �09 & 36213-00 to �09

115 VAC � 60 Hz, 3.8 Full Load Amps1/3 H.P. 1625 RPM Automatic ThermalProtection

Cabinet Model Electrical Requirements36205-10 to �1936213-10 to �19

115 VAC � 50/60 Hz, 3.9 Full Load Amps1/3 H.P. 1625 RPM Automatic ThermalProtection

Electrical Data

Motor Specifications


Appendix C: Biosafety Cabinet Specifications

Cabinet Model Electrical Requirements36209-10 to �19 115 VAC � 50/60 Hz, 6 Full Load Amps

1/2 H.P. 1625 RPM Automatic ThermalProtection

Cabinet Model Electrical Requirements36205-20 to �2936213-20 to �29

230 VAC � 50/60 Hz, 1.9 Full Load Amps1/3 H.P. 1625 RPM Automatic ThermalProtection

Cabinet Model Electrical Requirements36209-20 to �29 230 VAC � 50/60 Hz, 2.8 Full Load Amps

1/2 H.P. 1625 RPM Automatic ThermalProtection

Environmental Conditions

• Indoor use only.• Maximum altitude: 6562 feet (2000 meters).• Ambient temperature range: 41° to 104°F (5° to

40°C).• Maximum relative humidity: 80% for

temperatures up to 88°F (31°C), decreasinglinearly to 50% relative humidity at 104°F(40°C).

• Main supply voltage fluctuations not to exceed±10% of the nominal voltage.

• Transient overvoltages according to InstallationCategories II (Overvoltage Categories per IEC1010). Temporary voltage spikes on the ACinput line that may be as high as 1500V for115V models and 2500V for 230V models areallowed.

• Used in an environment of Pollution degrees 2(i.e., where normally only non-conductiveatmospheres are present). Occasionally,however, a temporary conductivity caused bycondensation must be expected, in accordancewith IEC 664.



C-1



C-2



C-3



C-4



C-5



C-6


AAPPPPEENNDDIIXX DDPPUURRIIFFIIEERR

AACCCCEESSSSOORRIIEESS

Safety Orange Air FoilBold orange color cautions the operator to avoidplacing materials in this area where airflow is critical.The air foil is epoxy-coated stainless steel and may befactory installed or retrofitted on site. Contact Labconcoat 800-821-5525, 816-333-8811 [email protected] for ordering information.

Base StandsEpoxy-coated steel, adjust in 1-inch increments toprovide a working height from 30 to 38 inches (29 ¼inches deep x 27 ½ to 34 ½ inches high). NSF testedand approved for use with the Purifier. ADAcompliant.

Catalog # For use with Width Shipping Weight37303-00 3-foot Purifier Delta Series 38 1/2" (98 cm) 73 lbs. (33 kg)37304-00 4-foot Purifier Delta Series 50 1/2" (128 cm) 82 lbs. (37 kg)37306-00 6-foot Purifier Delta Series 74 1/2" (189 cm) 100 lbs. (45 kg)


Appendix D: Purifier Accessories

Service Valve Kit(# 37475-00)Includes serrated hose tip valve with quarter turnhandle, hardware and instructions for plumbing toservices mounts on left or right side interior. Allcabinets are factory prepared to accept up to 4 fixtures.Shipping weight 4 lbs. (2 kg).

Ultraviolet Lamp KitsIncludes 254 nm UV lamp. Kit # 37450-00 alsoincludes a 115 volt ballast.

Catalog # For use with Shipping Weight37450-00 3- & 4-foot Purifier Delta Series 5 lbs. (2.3 kg)37450-01 6-foot Purifier Delta Series 3 lbs. (1.4 kg)

IV Bar KitsBar supports intravenous solution bottles and bags. Kitsinclude IV bar, mounting hardware, and four hangers.

Catalog # For use with Shipping Weight37455-00 3-foot Purifier Delta Series 3 lbs. (1.4 kg)37455-01 4-foot Purifier Delta Series 4 lbs. ( 1.8 kg)37455-02 6-foot Purifier Delta Series 6 lbs. (2.7 kg)



Canopy KitFor thimble ducting the Purifier Delta Series Cabinet tothe outside.

Catalog # For use with Duct Diameter Shipping Weight37307-00 3-foot Purifier Delta Series 10" 15 lbs. (7 kg)37307-01 4-foot Purifier Delta Series 10" 15 lbs. (7 kg)37307-02 6-foot Purifier Delta Series 10" 15 lbs. (7 kg)

B3 Conversion KitFor hard ducting the Purifier Delta Series Cabinet to theoutside.

Catalog # For use with Duct Diameter Shipping Weight37308-00 3-foot Purifier Delta Series 10" 20 lbs. (9 kg)37308-01 4-foot Purifier Delta Series 10" 20 lbs. (9 kg)37308-02 6-foot Purifier Delta Series 10" 20 lbs. (9 kg)

Remote Blower (# 36680-00)The 1/4 HP motor delivers between 650 CFM @ 0.38"static pressure, and 550 CFM @ 0.5". Belt drive withadjustable sheave. Phenolic coated impeller. Epoxy-coated steel base and weathercover. Integral backdraftdamper. Inlet accepts 10" diameter duct. Outlet accepts8" diameter duct. Maximum duct run of 100 equivalentfeet is recommended. Shipping weight 93 lbs. (42 kg).

Ergonomic Chair withArmrests (# 37440-00)Designed to Biosafety Guidelines. Lab chair has 6-wayarticulating seat and back control for personalizedadjustment. Pneumatic mechanism adjusts seat height



from 18 1/4" to 25 3/4". Five-leg black reinforcedcomposite base rests on 2" ball bearing casters.Aluminum support ring. Removable arm rests. Blackvinyl upholstery. Shipping weight 35 lbs. (15.9 kg).

Adjustable Footrest(# 37460-00)Elevates feet and permits angle repositioning while inuse. 18 1/2"w x 11 1/2"d x 8"h. Shipping weight 6 lbs.(2.7 kg).


AAPPPPEENNDDIIXX EEQQUUIICCKK CCHHAARRTT FFOORR

TTHHEE DDEELLTTAA

PPUURRIIFFIIEERRSS

Model 36204 36205 36208 36209 36212 36213Type A/B3 A/B3 A/B3 A/B3 A/B3 A/B3

Sash Height (inches) 10 8 10 8 10 8NSF Listed Downflow (FPM) 50-60 50-60 50-60 50-60 50-60 50-60

Sash Open Area (ft2) 2.53 2.03 3.37 2.69 5.03 4.03*Direct Read NSF Listed Inflow (FPM) 100-110 100-110 100-110 100-110 100-110 100-110

*Direct Read Exhaust Volume (CFM) 253-278 203-223 337-370 269-296 503-554 403-443Type B3 Exhaust Volumes (CFM) 253-278 203-223 337-370 269-296 503-554 403-443

Type B3 Alarm Setting Volume (CFM) 228 ±5 183 ±5 303 ±5 242 ±5 453 ±5 363 ±5Canopy Exhaust Volume (CFM) 278-306 223-245 371-408 296-325 553-609 443-488

Approximate Settings for new FiltersMotor Voltage 88-90 85-87 100-102 96-99 87-90 85-88

Mag Gauge Reading 0.8-1.1 0.8-1.1 0.8-1.1 0.8-1.1 0.8-1.1 0.8-1.1Total Air Volume Displacement (CFM) 548 601 728 798 1088 1193

Number of Laskin Nozzles Needed 1 1 2 2 2 2Supply HEPA Filter Dims. (in.) 36x18x3 36x18x3 48x18x3 48x18x3 72x18x3 72x18x3Exhaust HEPA Filter Dims (in.) 12x18x12 12x18x12 24x18x12 24x18x12 30x18x12 30x18x12

Motor HP 1/3 1/3 1/3 1/3 2 ea. 1/3 2 ea. 1/3Speed Winding Used High High High High High HighCapacitor (uF, Volts) 15 15 15 15 2 ea. 15 2 ea. 15Fluorescent Light(s) F25T8/

TL741F25T8/TL741

F32T8/TL741

F32T8/TL741

F72T12/CW

F72T12/CW

UV Light(s) G30T8 G30T8 G30T8 G30T8 G64T5L/CB

G64T5L/CB

Secondary Inflow Method DataSash Height (in.) 3.95 3.95 2.97 2.97 1.99 1.99

Distance Between Points 4.07 4.07 4.05 4.05 4.03 4.03Number of Test Points 8 8 11 11 17 17

Purifier Correction Factor 1.08 1.06 1.13 1.12 1.30 1.24

*The Direct Read instrument is sealed to the face or theexhaust opening. The following calculation is used:Air Volume (CFM) / Sash opening area (Ft2) = AverageFace Velocity (FPM).


Appendix E: Quick Chart for the Delta Purifiers

**For further details on the secondary inflow method,consult the inflow velocity calculation � SecondaryMethod section of Chapter 6: Maintaining YourPurifier.

Use the downflow velocity test grid as described on thecabinet data plate when checking the downflow airvelocity. The individual downflow velocity readingsmay vary no more than ± 20% from the downflowaverage.


AAPPPPEENNDDIIXX FFRREEFFEERREENNCCEESS

Many excellent reference texts and booklets arecurrently available. The following is a brief listing:

Primary Containment for Biohazards: Selection,Installation and Use of Biological Safety Cabinets.September 1995. U.S. Department of Health andHuman Services, Public Health Service, Centers forDisease Control and Prevention and National Institutesof Health.

Biosafety in Microbiological and BiomedicalLaboratories. 4th Edition, May 1999. U.S. Departmentof Health, Education, and Welfare, National Institutesof Health, Division of Safety, Bethesda, MD 20892.

Biosafety in the Laboratory. 1989. National ResearchCouncil, National Academy Press, Washington, D.C.

Laboratory Safety: Principles and Practices. 2ndEdition 1995. American Society for Microbiology,Washington, D.C.

National Sanitation Foundation Standard Number 49for Class II (Laminar Flow) Biohazard Cabinetry.1992, Ann Arbor, Michigan.

ASHP Technical Assistance Bulletin on HandlingCytotoxic and Hazardous Drugs. 1990. AmericanSociety of Hospital Pharmacists. American Journal ofHospital Pharmacy. 47: 1033-1049.

The Foundations of Laboratory Safety. 1990. StephenR. Rayburn. Springer-Verlag, New York.


Appendix F: References

Formaldehyde Decontamination of Laminar FlowBiological Safety Cabinets. U.S. Department of Health,Education, and Welfare, National Institutes of Health,Division of Safety, Bethesda, MD 20892.

Websites of Interest:

www.absa.orgwww.cdc.govwww.labconco.com


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