Upload
todd-rosario
View
31
Download
5
Tags:
Embed Size (px)
DESCRIPTION
Cercarial Dermatitis in Austria Status of Research. Christoph Hörweg 1 , Herbert Auer 2 & Helmut Sattmann 3 1 Institute of Zoology, Department of Systematic Zoology, University of Vienna, Austria - PowerPoint PPT Presentation
Citation preview
Cercarial Dermatitis in Austria Status of Research
Christoph Hörweg1, Herbert Auer2 & Helmut Sattmann3
1 Institute of Zoology, Department of Systematic Zoology, University of Vienna, Austria2 Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna3 Department of Invertebrates, Natural History Museum Vienna, Austria
Cercarial Dermatitis in Austria
First Record
Gernot Graefe (1971):„Experimenteller Nachweis einer von Cercarien verur-sachten Dermatitis am Neusiedlersee.“
(Sitzungsber. d. Österr. Akad. d. Wissenschaften, Mathemat.-naturw. Kl., Abt. 1, 179: 73-79)
Trichobilharzia szidati
Cercarial Dermatitis in AustriaGeographical Distribution
Vorarlberg: no data
Tyrol: Natterersee (1992): CD, Furcocercariae
Salzburg: Wallersee, Wolfgangsee (1994): CD Badesee Gastein (2002): CD Wallersee (2002): Trichobilharzia sp.
(Stagnicola fuscus)
V T S T
Cercarial Dermatitis in AustriaGeographical Distribution
Upper Austria: Attersee (1992): CD Some lakes around Linz (2002): CD
Carinthia: Wörthersee, Weißensee, etc. (1950 – 90): CD Herzogstuhl (2002): ocellate Furcocercariae
(Gyraulus parvus)
Styria: Some lakes around Graz (1994): CD,
Trichobilharzia ocellata (Radix auricularia)
Weihermühle (2002): CD, Furcocercariae
UA
C
St
Cercarial Dermatitis in AustriaGeographical Distribution
Burgenland: Neusiedlersee (1968-70): CD, T. szidati (L.
stagnalis), Bilharziella polonica (Planorbarius corneus)
Neusiedlersee (1998): B. polonica (P. corneus) Vienna:
Lobau (1983): CD, T. ocellata (L. stagnalis) Lobau (1993, 1994): CD, Furcocercariae Ölhafen (1997): T. ocellata (L. stagnalis, R.
ovata) Alte Donau (2001, 2002): CD Neue Donau (2003): CD
B
V
Cercarial Dermatitis in AustriaGeographical Distribution
Lower Austria: Fischamend/Donau (1971): CD, T. szidati (L.
stagnalis) Sitzenberg (1973): CD, T. szidati (L. stagnalis,
R. auricularia) St. Veit a.d. Gölsen (1983): CD Stopfenreuther Au (1994): CD Wienerwald/Rekawinkel (1998): T. szidati (L.
stagnalis) Schönau/Donau (1998): Trichobilharzia sp. (R.
peregra) Mannswörth (2002): T. sp. (Stagnicola fuscus) Achau (2002): CD Fischamend/Donau (2002): T. sp. (Aplexa
hypnorum)
LA
Cercarial Dermatitis in Austria Summary - Status
2003
Causative agents: Bilharziella polonica Trichobilharzia
szidati Trichobilharzia
ocellata (?)Trichobilharzia sp. (?)
Intermediate Hosts: Lymnaea stagnalis Radix auricularia R. ovata Stagnicola fuscus Planorbarius corneus Gyraulus parvus Aplexa hypnorum
Final hosts: ?
Cercarial Dermatitis in Austria
Alte Donau / Vienna 2002 Date: 21.06.2002, Time: 2 p.m., Weather: hot & sunny
32°C
27°C
Cercarial Dermatitis in Austria
Alte Donau / Vienna 2002
Number of snails: 86 (from 6 collection sites) 6 Radix sp., 36 Planorbis planorbis 23 Bithynia tentaculata, 20 Physa acuta
=> no cercariae found with “cercarial release experiments”
Conclusion: more local/specific collections (dependent on
occurrence of cercarial dermatitis) needed Sample size should be extended
Cercarial Dermatitis in Austria
Achau / Lower Austria 2002
Date:03.07.2002
Time: 2 p.m.
Weather: sunny + windy
25°C
27°C
Cercarial Dermatitis in Austria
Achau / Lower Austria 2002
Cercarial Dermatitis in Austria
Achau / Lower Austria 2002
Number of snails: 4 (collected one week before) 4 Radix ovata
=> only echinostome cercariae found => but massive CD Conclusion:
Snail population maybe broken down after continuous heat (many empty shells) or snails in mud in deeper regions (2,5 – 3 metres)
Cercarial Dermatitis in Austria
Wöllersdorf / Lower Austria 2003
Date:11.06.2003
Time: 2.30 p.m.
Weather: hot & sunny
34°C
24°C
Cercarial Dermatitis in Austria
Wöllersdorf / Lower Austria 2003
Number of snails: 83 (collected the last days) 63 Planorbarius corneus 5 Lymnaea stagnalis, 3 Stagnicola sp., 1 Radix sp.
=> no cercariae found with “cercarial release experiments”
Conclusion: Sample size should be extended, most likely some
more snails are within the many water plants
Cercarial Dermatitis in Austria
Molecularbiology – Preliminary results
A specific PCR for the amplification of the ITS regions and 5.8S rRNA gene from cercariae and sporocysts in the digestive glands of snails has been established.
High sequence similarities of isolates within each host species and significant dissimilarities between isolates of different host species could be determined.
Cercarial Dermatitis in Austria
Project applied - Objectives
SYSTEMATICS & TAXONOMY
To install a method for the differentation of intermediate trematode stages and host snail taxa
To create an inventory of the species spectrum of the schistosomes which cause cercarial dermatitis and of the intermediate host snails
To record snail host specificity and the host spectrum of schistosomes
Cercarial Dermatitis in Austria
Project applied - Objectives
EPIDEMIOLOGY & ECOLOGY
To study the epidemiology and community structure of trematodes in selected snail species
To record the population dynamics of snails and the development of schistosomes within snails
To find correlations between population dynamics and diversity of hosts and parasites
Cercarial Dermatitis in Austria
Project applied - Objectives
ECONOMY & MEDICINE
To ensure the proper diagnosis of cercarial dermatitis
To generate guidelines for a risk evaluation and to develop an early warning system
To propose a catalogue of measures to minimise the risk of cercarial dermatitis
Cercarial Dermatitis in Austria
THANKSFOR YOURATTENTION
!
NO MORE !
VISIT THE NHM VIENNA !!
NO MORE !