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8/14/2019 Cell & Tissue Biology Laboratory Exercise 6: Blood Block
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Cell & TissueCell & Tissue
BiologyBiologyLaboratory Exercise 6:Laboratory Exercise 6:
BloodBloodBlock Two, 2009Block Two, 2009
8/14/2019 Cell & Tissue Biology Laboratory Exercise 6: Blood Block
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Slide 5 peripheral blood,Giemsa
1. Some of the RBCs have a pale staining center, why?
Because there is less cellular material in this part.
1. What is the function of the erythrocytes biconcave shape?
It increases the surface-to-volume ratio for increased gas exchange.
1. What cytoskeleton elements maintain the erythrocytes shape?
Actin, ankyrin, and spectrin
1. What does the effect of the missing nucleus have on the life span of the erythrocyte?
It has a limited lifespan of 120 days.
1. Based on what youve learned about the functions of erythrocytes, what conditions
might produce the preature release of reticulocytes from the bone marrow into the
peripheral blood?
Hemorrhage, anemia, and increased altitude.
8/14/2019 Cell & Tissue Biology Laboratory Exercise 6: Blood Block
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Slide 5 peripheral blood,Giemsa
NeutrophilNeutrophil
Neutrophils are approximately 10 m
in diameter. Also note the unstained
cytoplasm of the neutrophils.
Neutrophilic granules do not stainwith either acidic or basic dyes.
If the blood sample on your slide was
from a female, one of the nuclear
lobes may have a drumstick-shaped
nuclear appendage.
1. List two substances within the non-
specific and specific granules of
neutrophils?
Non-specific:Non-specific: acidic hydrolyases,
lysozyme & other enzymes
Specific:Specific: alkaline phosphatase,
aminopeptidase & collagenase
1. What is the nuclear appendage in
female neutrophils?
Barr bodies
1. What are known functions ofneutrophils?
They are microphages kill
bacteria / parasites through
phagocytosis. They also initiate
the inflammatory response.
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Slide 5 peripheral blood,Giemsa
EosinophilsEosinophils
Eosinophils make up ~1-4% of the
WBC population. They are slighly
larger than neutrophils and have
reddish (eosinophilic) granules within
their cytoplasm. Their cytoplasm
should be darker red or orange
compared to that of RBCs.
A bi-lobed nucleus is a characteristic
feature of eosinophils The specific
(secondary) granules contain major
basic protein (MBP) and other basic
proteins that combat parasites.
Eosinophils function outside of the
circulation in which tissues? Why are
they in the tissue space rather than in
the blood vessels?
Found in the dermis and the
connective tissue components of
the respiratory tree, GI tract,
and uterus & vagina. They are
found in the tissues because
thats where the microorganisms
are they need to fight.
1. List the functions of eosinophils.
Kill parasites
Phagocytose Ab:Ag complexes
Secrete leukotrienes for the
inflammatory response.
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Slide 5 peripheral blood,Giemsa
BasophilsBasophils
Basophils make up
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Slide 6 bone marrow, Wright
1. What is the function of platelets?
They are involved in blood clot
formation.
1. How many nuclei are present in
platelets?
zero.
PlateletsPlatelets
Look between the RBCs and you will
see fine dark particles, which will
look like debris. These purple specks
are platelets and may occur singly or
in small groups.
Platelets are also called thrombocytes.
Thrombocytopenia is a condition in
which there is an abnormally small
number of platelets in the peripheral
blood.
1. What do you suppose is the principal
symptom of thrombocytopenia?
Excessive bleeding.
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Slide 6 bone marrow, Wright
1. How many nuclei are present in
megakaryocytes?
one.
MegakaryocyteMegakaryocyte
This is a VERY large cell (~100 m
in diameter) with a lobed nucleus.