Cell Impedance Measurement System: Cell Culture and Interface

Board Design

Emma Berry & Austin GrimesDr. Nicole McFarlane, Khandaker Mamun, Geneva Doak

YSP/RET Final PresentationsJuly 18, 2013

Knoxville, Tennessee

Cell Impedance Measurement System

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Cell Culturing Method

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Cell Captures

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Subculture

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Neutral Red

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Printed Circuit Boards(PCBs)

• Printed Circuit Boards are circuits that are compacted into a small board.

• Printed Circuit Boards are used to connect a device, or cells, to an interface like the lock in amplifier.

Printed Circuit Boards(PCBs)

• The circuit boards we have created have all been created in Eagle, a software that is specifically made for designing PCBs.

• In Eagle, you must start off by making a schematic for the software to know what connects to what.

BNC Port

10 Pin Sockets

8 Pin Switch

Printed Circuit Boards(PCBs)

• Once you are done with the schematic, you press a button that transfers you to the board layout.

• The pieces are off to the side and you put them on to the board where you want them to go.

• Although this may sound easy, it is like a complex puzzle.

BNC Port

10 Pin Socket

8 Pin Switch

Printed Circuit Boards(PCBs)

• Once you have everything where you want it on the board, you add a silkscreen.

• The silkscreen tells the board factory what you need to be printed on the board.

Printed Circuit Boards(PCBs)

• When we receive the board from the factory, we will need to solder any pieces onto the board that we need for our studies.

BNC Port

10 Pin Socket8 Pin Switch

Printed Circuit Boards(PCBs)

• Once we solder the pieces on to the board, it is ready to test with the cells.

8 Pin Switch

BNC Port

10 Pin Socket

Results

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0 5 10 15 20 25 300

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40

60

80

100

120

140

21.5 k HzPolyfit (21.5 kHz)28.9 kHzPolyfit (28.9 kHz

Time (Hours)

Impe

danc

e (Ω

)

Conclusion

• Contributions successful• Future work

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Acknowledgements

• We thank Dr. Chien-fei Chen, Dr. Nicole McFarlane, Khandaker Abdullah Al Mamun, and Geneva Doak for their guidance and encouragement during this project. We also thank CURENT for providing this program and experience for young scholars.

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References

Freshney, R. Ian. Culture of Animal Cells: A Manual of Basic Technique. New York: Wiley, 2000.

Hamilton, T.J.; Nelson, N.M.; Sander, D.; Abshire, Pamela, "A cell impedance sensor based on a silicon cochlea," Biomedical Circuits and Systems Conference, 2009. BioCAS 2009. IEEE , vol., no., pp.117,120, 26-28 Nov. 2009

Prakash, S.B.; Nelson, N.M.; Haas, A.M.; Jeng, V.; Abshire, Pamela; Urdaneta, M.; Smela, Elisabeth, "BioLabs-On-A-Chip: Monitoring Cells using CMOS Biosensors," Life Science Systems and Applications Workshop, 2006. IEEE/NLM , vol., no., pp.1,2, July 2006

Reeves, N.; Liu, Y.; Nelson, N.M.; Malhotra, S.; Loganathan, M.; Lauenstein, J.-M.; Chaiyupatumpa, J.; Smela, E.; Abshire, P.A., "Integrated MEMS structures and CMOS circuits for bioelectronic interface with single cells," Circuits and Systems, 2004. ISCAS '04. Proceedings of the 2004 International Symposium on , vol.3, no., pp.III,673-6 Vol.3, 23-26 May 2004

Urdaneta, M.; Christophersen, Marc; Smela, Elisabeth; Prakash, S.B.; Nelson, N.; Abshire, Pamela, "Cell Clinics Technology Platform for Cell-Based Sensing," Life Science Systems and Applications Workshop, 2006. IEEE/NLM , vol., no., pp.1,2, July 2006

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