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CELL CULTURE AND CELL BIOLOGY
OUR BRANDS101Bio, Bertin BioReagent, Biopredic, Biotium, BPS Biosciences, Canvax, Cayman, Cell Bio-logics, Corning, Corning Media, Eurobio, Fal-con, Innovative Cell Technologies, PAN-Biotech, Polyplus transfection, Polysciences, Prospec, Reprocell, ScienCell, Stemgent, Torrey Pines
3D cell culture
¸ Hydrogels for 3D cell-culture → Matrigel® matrix → Col-TGel (101Bio): collagen based gel without growth factors and
with 3 different stiffness to fit most cell types
¸ Porous membranes → Transwell® inserts and HTS Plates → Alvetex scaffold: natural tissue-like structure that enables the cells
to function in a more physiologically relevant pattern
¸ Ultra Low Attachment (ULA) microplates: 3D spheroid formation and generation of a single 3D uniform spheroid per well with reproducible size
Dear customer,
discover our large product range for your Cell Culture & Cell Biology product needs. Our icon meanings:
Information about additional documentation
Order your sample at [email protected]
; You can now download your certificate of analysis on our website under Support
Your Chemie Brunschwig AG Team
FBS and Sera
¸ Classical Fetal Bovine Sera → several origins (South America, USA, Australia, …) → different treatments (i.e. tet-negative, ultra low IgG, heat-inactivated, dialyzed, delipidized, …)
¸ FBS Supreme, standardized Fetal Bovine Serum: single components naturally present in the serum are enriched in a higher quality leading to a more defined product; time consuming for batch testing would not be necessary anymore.
¸ FBS Active, in this product 80% of the FBS is replaced by chemically defined components. The reproducibility of your cell culture experiments or processes can be improved tremendously.
¸ Panexin Basic & Panexin NTA are fully defined serum replacement for the cul-tivation of adherent and non-adherent cells. Support the growth of many cell types in an optimum manner without any extra handling compared to serum.
¸ Filtrated Bovine Serum, a cost effective alternative to FBS suitable for many cells types ¸ Human and animal Sera
Animal free systems
¸ Serum free media → for different cell types (HEK-293, CHO, keratinocytes, Vero cells, lympho-
cytes, hybridoma cell lines,…) → for routine cultures or bioprocesses (protein, antibody or virus production)
¸ Panexin CD is a full chemically defined new serum replacement for the cultiva-tion of adherent and non-adherent cells that shows a high reproducibility from lot to lot and simplified downstream processing.
¸ Human platelet lysate (hPL) is a xeno-free alternative to FBS as well as human serum for in vivo wound healing and tissue repair, but also for the expansion of human cells in vitro.
see & download PAN catalog 2018-2019 on website under suppliers
Media, Supplements, Buffers & Reagents
¸ Broad range of classical media (DMEM, RPMI, Ham’s F-12, …) → in liquid or powder form → various formulations and pack sizes
¸ All reagents and buffers for cell culture ¸ Cell dissociation
→ classical enzymes (Collagenase, Trypsin) → Accutase® a smooth alternative to trypsin for sensitive cells, FACS or receptor studies
Free sample available - ask yours! ¸ Growth factors: hormones, cytokines, chemokines: human or animal ¸ Cell specific media: basal media or kits incl. media + pre-tested FBS + growth suppl. ¸ Customized media
Cells and Tissues
Transfection
¸ DNA transfection reagent: JetOPTIMUS®
→ high efficiency → cost-effective → also suitable for hard-to-transfect-cells
¸ siRNA/miRNA transfection reagent: INTERFERin®
→ high Silencing efficiency → low off-target effects
¸ mRNA transfection reagent: jetMESSEGER®
→ unmatched transfection efficiency → extremely gentle on the cells
¸ RNP (Cas9 protein+gRNA) transfection reagent: jetCRISPR®
→ high genome editing efficiency → maintained cell viability
¸ in-vivo nucleic acid delivery: In-vivo JetPEI®
→ easy and safe delivery method → convenient to any animal model
¸ Bioproduction: PeiPRO, FectoCHO/PRO®
→ Protein production → Virus production
DNA
mRNA
Ribonucleoprotein (RNP)
jetOPTIMUS® – DNA method• Plasmid DNA encoding both Cas9 and
gRNA
jetMESSENGER® - RNA approach• mRNA encoding Cas9 and gRNA
jetCRISPR® - RNP Delivery• Cas9 protein complexed with gRNA
¸ Cells → Human and animal primary cells, fresh or frozen suitable
for R&D, drug discovery, pharmacology & toxicology ¸ Hepatocytes
→ Fresh or cryopreserved → Human and Animal → HepaRG™, the human hepatic stem cell line
¸ Human and animal tissues → Skin Tissues → cells and fractions for permeability, toxicology or
metabolism research
¸ Biologic fluids
¸ SILENSOMES One single model for DMPK phenotyping studies
Supplies for Laboratory and Prodcution
Chemie Brunschwig AGAuf dem Wolf 10CH-4052 Basel
T +41 61 308 91 [email protected]
Cell biology assays
¸ Viability Study: Resazurin, MTT, XTT, ATP-Glo ¸ Apoptose: Caspase, Annexin, TUNEL assays ¸ Reporter gene assays: firefly/renilla luciferase assays, Galactosidase assays,
SEAP reporter gene assays ¸ Metabolite quantitation Kits: cholesterol, free fatty acids, triglycerides, … ¸ Stress oxidative assays ¸ ELISA kits: Ready-to-Use or Do-It-Yourself kits ¸ Organel studies:
→ mitochondries (MitoView, JC-1, …) → nucleus (DAPI, Red-Dot, Hoechst dyes, …) → lysosome (LysoView) → cytoskelet (Phalloidins)
¸ Fluorescent ion and pH indicators ¸ Membrane potential dyes
Disposable labware
¸ High-quality disposable plastic ware for cell culture → Flasks, dishes, microplates, scrapers, … → Different treatments available for adaptability to any cell types: Not-treated, TC-treated,
Ultra Low Attachement, pre-coated ¸ Liquid handling
→ pipettors, pipet controller, aspirators, plate washer, serological pipets, filtration systems, centrifuge tubes, bottles, sterile racked filter tips,…
¸ Plastic ware for Bioprocess → Erlenmeyer (from 125ml to 5L), stackable cell culture flasks (up to 25‘440cm2), Roller bottles,
Bioreactors (from 50ml to 36L), Microcarriers for bioprocess scale-up: available with 4 surface treatments
¸ Cryogenic vials and accessories → cryogenic vials with external or internal thread, racks and storage boxes, CoolCell, CoolBox and
CoolRacks for a consistent and reproducible cooling or temperature control ¸ Cell Strainers for cell isolation from tissues ¸ Benchtop equipment
→ centrifugation equipment (micro- or mini-centrifuge, high speed micro centrifuge, compact centrifuges, …)
→ vortex mixers, platform or 3D shakers, Tube roller or rotator, Magnetic & hotplate stirrers
→ Cell Counter
CB0
719
