Cc1 Notes Intro

8/17/2019 Cc1 Notes Intro

1/27

TERMINOLOGIES

• Laboratory Medicine

Discipline involved in the selection,provision & interpretation ofdiagnostic testing that uses priaril!saples fro patients


2/27

Testing involves:

Confrming a clinical suspicionExcluding a diagnosis

"ssisting in the selection,optimization & monitoring oftreatent

#roviding a prognosis

Screening for a disease in thea$sence of clinical signs ands!ptos


3/27

Reference Standard

Single test or a co$ination

of ethods and techni%uesincluding clinical follo'up oftested patient

(est availa$le ethod foresta$lishing the presence ora$sence of target condition


4/27

Core Laboratories#rovide high' volue & eergenc!

testing in an! hospitals

Depends on )

"utoation

Inforatics

*oputers+ualit! control

+ualit! anageent


5/27

CLINICAL CHEMISTRY (ranch of edical la$orator! sciencehich deals ith the %uantitativeanal!sis of $iocheical productsfound in $iological uids such asseru, plasa, urine or spinal uid-


6/27

Iportance.acilitate the correct

perforance of anal!ticalprocedures that !ield accurateand precise inforation,aiding patient diagnosis andtreatent


7/27

Roles of Medical Technologist

Ensures achieveent of relia$le results$!)

*orrectl! using $asic supplies ande%uipent

/noing the di0erent $iocheicalsu$stances in ters of their nature,eta$olis, reference ranges and

varia$les that a! a0ect their anal!ses*orrelate test results ith pathologic

conditions

#erforing $asic la$orator! calculations


8/27

#racticing %ualit! assurance and

la$orator! safet!#erfor correctl! la$ assa!s used to

easure concentration of speci1c

anal!teSolve coon pro$les encountered in

the clinical la$orator!

Manifest professional $ehavior and

attitudes


9/27

CHEMICALS


10/27

suita$le for use in ost anal!tic la$procedures

Meet the speci1cations set $! "erican*heical Societ! 2"*S3

Analytical Reagent Reagent !rade


11/27

"ltra #$re additional puri1cation stepssuch chroatograph!, atoica$sorption, iunoassa!s orother techni%ues that re%uiree4treel! pure cheicals

5#L* or chroatographicla$eled


12/27

Che%ically #$re

Ipurit! liitations are not

stated6 preparation is notunifor

Not recoended for

reagent preparation in clinla$s


13/27

7S# 27nited States #haracopoeia3N. 2 National .orular!3

7sed to anufacture drugs6

liitations esta$lished are $asedonl! on that of not $eing in8uriousto health

Ma! or a! not eet all assa!re%uireents


14/27

Technical Grade

7sed priaril! in anufacturingand should not $e used in theclin la$


15/27


16/27

#ri%ary standard

5ighl! puri1ed cheical6 can $eeasured directl! to produce asu$stance of e4act 9non

concentration & purit!Secondary standard

Of loer purit!


17/27

Standard Reference MaterialDeveloped $! National Institute

of Standards and Technolog!2NIST3

*erti1ed cheical coposition$ut a! not possess the purit!e%uivalent of a priar! standard

Often used to verif! cali$rationor accurac!:$ias assessent

7sed in the anufacture ofcali$rators and standard

aterials


18/27

&ATER S#ECI'ICATI(NS


19/27

)istilled *ater

E0ectivel! reoves $acteria, p!rogens,particulate atter, dissolved ioni;edsolids

)eioni+ed *ater

#repared fro ion e4change resin

E4cellent for reoval of dissolved ionsderived fro gases and solids $ut is

ine0ective for all other containantsDoes not reove particulate atter,

$acteria or p!rogens


20/27

RE


21/27

7ltra1ltration

Reoves particulate atter,eulsi1ed solids, ost$acteria & p!rogens

Does not e0ectivel! reovedissolved ioni;ed solids &gases & ost organic

containants


22/27

"ltraviolet o,idation andsterili+ationReove trace %uantities of

organiccontainants2o4idation3&$acteria2sterili;ation

Di0erent avelengths are used

for these processes' =>? n foro4idation & @?A n forsterili;ation


23/27

(+one7sed priaril! in industrial

settings6 e0ectivel! reovesorganic containants

/ills $acteria $! rupturing thecell e$rane alostinstantaneousl!2 @ secs3


24/27

REA!ENT !RA)E&ATER


25/27

B *"TEGORIES 2*LSI3)

*LRC2 *linical La$orator! Reagent Cater3Meet the speci1cations for ost routine

clin la$ use

Electrophoretic anal!ses, to4icolog!

screening procedure, preparation of$u0ers, standards and controls

SRC2 Special Reagent Cater

I.C 2Instruent .eed Cater3"CC 2"utoclave and Cash Cater

Cater supplied $! ethod anufacturer

*oerciall! $ottled puri1ed ater


26/27

Ty-es of &ater

T!pe I5ave the ost stringent re%uireent &

generall! suita$le for routine la$ use

.or test ethods re%uiring iniuinterference such as trace etal, ironand en;!e anal!ses

T!pe II

.or %ualit! control standard preparation t!pe III

.or glassare ashing


27/27

Testing #rocedures to deterine the%ualit! of reagent grade ater

Measureents of resistivit!

"ssess the ionic content of puri1ed ater

p5 , hardness*olon! counts

*hlorine

"onia, #OA, sodiu silica

Nitrite or nitrate, *O@, cheical o4!gendeand

Iron, etal detection

Documents

Cc1 Notes Intro