S70 Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143

The microelements dynamics in a collection of varieties ofAlfalfa in the Romanian Banat plain conditions

Dorin Gaitin, Monica Butnariu, Ionel Samfira

Agricultural Technologies Faculty of Agriculture, Banat University ofAgricultural Sciences and Veterinary Medicine, Timisoara, RomaniaE-mail address: sionel1969@yahoo.com (D. Gaitin).

Medicago sativa L. is one of the species that improve the soil char-acteristics, increasing the organic matter content, limiting soilerosion, and improving its structure.

Purpose of this work was to track the dynamics of macro andmicroelements in alfalfa during its vegetation period. The biologicalmaterial was represented by these alfalfa varieties: Victoria, Pom-posa, NS Banat ZMS II and Magnat. Experimental field was locatedin the Banat plain, on a soil with a pH of 7.70, total nitrogen 0.23and mobile phosphorus at 177 ppm. We studied the dynamics ofnitrogen content, phosphorus, potassium, copper, nickel, lead, zinc,calcium, magnesium and iron in plants in relation to soil chemicalcharacteristics. The total nitrogen content was obtained throughKjedadl method, potassium, phosphorus and trace elements wereobtained by spectrophotometrically method.

Note that the variety Victoria has the highest nitrogen content,phosphorus and potassium, but associated with the largest quanti-ties of lead, iron and zinc plant. Pomposa variety is low in nitrogenbut it is associated with a high content of phosphorus contributingto increased plant resistance.

After our study Victoria believe that variety is the most adaptedto the culture of the Banat plain, valuing the high level of macroelements in the soil reserve.

http://dx.doi.org/10.1016/j.copbio.2013.05.190

The impact of industrial residues dumps on environment

Narcis Baghina 1, Ciprian Stroia 2, Isidora Radulov 3, AdinaBerbecea 3, Ioan Gaica 1, Florin Crista 3

1 Environmental Engeeniring and Sustainable Developement, Banat’sUniversity of Agricultural Sciences and Veterinary Medicine FromTimisoara, Timisoara, Romania2 Biology, Banat’s University of Agricultural Sciences and VeterinaryMedicine From Timisoara, Timisoara, Romania3 Soil Science, Banat’s University of Agricultural Sciences and Veteri-nary Medicine From Timisoara, Timisoara, RomaniaE-mail address: narcis baghina@yahoo.com (N. Baghina).

The largest quantities of industrial waste are stored in waste dumpsand tailing ponds representing major environmental hazards dueto instability of dumps. In order to study pollution of a dump inBanat Area, samples were taken tailings disposal pond 1, pondtailings discharge; mine wastewater disposal, storage lake; minewater, mine water discharge, discharge wastewater, mine waste-water discharge from ponds unified, sewage lagoons dischargeof copper and pyrite. Experimental data for residues exceedingthe MAL fixed in all water samples analyzed. This means thatwaste water have a significant residual load. Iron content is inthe range 0.19–1.00 mg/l. Iron ions in water samples over timedue to solubilisation of iron containing ores. Manganese ionscontent exceeds maximum of 0.25 mg/l in water samples col-lected from wastewater mine. Cu2+ ion content is in the range0.001–0.09, below the MAL. Zn2+ ion is between SLD and 1 mg/l,exceeding approx. 3–4 times the MAL. Ni2+ ions content in allsamples fall below the specified maximum permissible level0.1 mg/l.

Acknowledgements: This work was published during theproject POSDRU/89/1.5/S/62371, co-financed by the EuropeanSocial Fund through the Sectorial Operational Programme for theHuman Resources Development 2007–2013.

http://dx.doi.org/10.1016/j.copbio.2013.05.191

BOD characterization of extraction and electrocoagulation pro-cess for the treatment of biodiesel wastewater

Nursel Kiratli Yilmazcoban, Ismail Ayhan Sengil, Meral Yurtsever

Environmental Engineering Department, Engineering Faculty, SakaryaUniversity, Sakarya, TurkeyE-mail address: nkiratli@gmail.com (N.K. Yilmazcoban).

The aim of present study is, to determine the hexane extrac-tion of raw biodiesel wastewater which was produced in thelaboratory, to determine the BOD characterization after the elec-trocoagulation with the Fe and Al electrodes and to calculatethe BOD kinetic parameters of k, k′ and Lo with the ThomsonGraph Method. k′ values of hexane extracted of raw biodieselwastewater (EBW), electrocoagulated of raw biodiesel wastewa-ter with the Fe electrodes (FeEBW), electrocoagulated of rawbiodiesel wastewater with the Al electrodes (AlEBW) sampleswere evaluated respectively as 0.242, 0.201 and 0.145. The Lovalues determined with the help of BOD experiment results arerespectively given as 755 mg/l, 283 mg/l and 655 mg/l. Bacte-ria was tried to be adapted to the environment approximatelyone day during each of the three wastewater treatments (LagPhase). Logarithmic phase period of bacteria in BOD curve wasvaried up to the wastewater character. The last results of BODstated that, FeEBW was more toxic than the EBW and theFeEBW.

http://dx.doi.org/10.1016/j.copbio.2013.05.192

Environmentally friendly technology wheat cultivation in areaswith soils contaminated with heavy metals

Ravilya Alybayeva, Saule Kenzhebayeva, Saule Atabayeva

Kazakh National University named after al-Faraby, KazakhstanE-mail address: raya aa@mail.ru (R. Alybayeva).

The aim of this study was to identify wheat germplasm is resis-tant to heavy metals (lead, copper, zinc and cadmium) that arepriority pollutants in eastern Kazakhstan region and identificationof donors for breeding and promising forms destined for agricul-tural production. Different genotypes of winter wheat the world’scollection (Kazakh, Russian, and a collection of CIMMYT varietiesand lines of winter wheat, wild species of wheat) as well as spe-cific to East Kazakhstan were studied. Field studies carried out forthe determination of physiological parameters. Heavy metals insoil and in plant samples were determined by atomic absorptionspectrophotometry. Genotypic differences in the accumulationwere established. The smallest number of studied heavy metalsaccumulates in the seeds of varieties of winter wheat Ming-2,Mironovskaya-808 and Krasnovodopadskaya-25. These genotypescan be recommended for further study in breeding process. Thehighest yield from plots has winter wheat Mironovskaya-808,116/271, Ming-2. A crop yield of plants is connected with theirability to quickly enter to the tillering stage, successfully overwin-ter, preserve during the summer vegetation. Varieties of winterwheat Ming-2 and Mironovskaya-808 can be recommended forcultivation in the technologically disadvantaged regions, with soilcontaminated by heavy metals, as this varieties accumulate not