Biotechnology Exam

7/25/2019 Biotechnology Exam

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Republic of the Philippines

Department of Education

Region XI

DIVISION OF T!"# $IT%

Tagum City National High School

Third Periodical E&amination

BIOTECHNOLOGY

Name'(((((((((((((((((((((((((((((((((((((((((((((Section' ((((((((((Score' ((((((((((((((((I) #ultiple $hoice) Read each item carefull*) $hoices are pro+ided at the left side before the number of each ,uestion)Shade the circle of the letter that corresponds to *our ans-er)

.) /hat does !#O stand for0

) !eneticall* modified organism

1) !rand mone* order

$) !ood mar2eting option

D) !enuine moti+ation onl*

3) The scientist used restriction

en4*mes for -hat purpose in the e&periment0

) To ma2e the plasmid small enough to transform cells

1) To ma2e cuts in the plasmid DN$) To ma2e the plasmid enter the cells

D) To enable the fragments of DN to form co+alent

bonds

5) /hat is the function of a

+ector in genetic engineering0

) cut DN into man* fragments

1) carr* DN into a ne- cell

$) lin2 together ne-l* 6oined fragments of DN

D) ma2e millions of copies of a specific segment of

DN

7) /hat is the function of DN

ligase in recombinant technolog*0

) ma2e millions of copies of a specific segment of

DN

1) carr* DN into a ne- cell

$) cut DN into man* fragments

D) lin2 together ne-l* 6oined fragments of DN

8) /hich best describes a

transgenic organism0) one that acts as the donor for DN to be mo+ed into

another organism

1) one produced b* cloning a mutant cell

$) one produced b* the pol*merase chain reaction

D) an* geneticall* modified organism resulting from

laborator* research

9) /hat process allo-s for the

+er* specific isolation and mo+ement of genes for

desirable traits0

) selecti+e breeding 1) genetic engineering

$) cloning D) microin6ection

7) /hat is the most logical

se,uence of steps for splicing foreign DN into a

plasmid and inserting the plasmid into a bacterium0

I) Transform bacteria -ith recombinant DN molecule)

II) $ut the plasmid DN using restriction en4*mes)

III) E&tract plasmid DN from bacterial cells)

IV) Insert the foreign DN fragments to the plasmid

DN)

V) "se ligase to seal plasmid DN to nonplasmid DN)

) I: II: IV: III: V 1) II: III: V: IV: I

$) III: II: IV: V: I D) III: IV: V: I: II

;) /hich of the follo-ing

produces multiple identical copies of a gene for basic

research or for large scale production of a gene product0

< restriction en4*mes 1< gene cloning

$< DN ligase D< gel electrophoresis

=) /hich of the follo-ing seals

the stic2* ends of restriction fragments to ma2e

recombinant DN0



.>) /hich of the follo-ing

cuts DN molecules at specific locations0

< restriction en4*mes 1< gene cloning$< DN ligase D< gel electrophoresis

..) /hich of the follo-ing

separates molecules b* mo+ement due to si4e and

electrical charge0




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.3) Restriction fragments of

DN are t*picall* separated from one another b* -hich

process0 < filtering 1>C*earCold preser+ed

s2in of an

e&tinct dodo a bird) /hat are

the currentbenefits of ha+ing foods made from

geneticall* modified crops0) The* impro+e farm profitabilit* and ma2e some

farmers@ 6obs easier)

1) The* allo- farmers to greatl* increase the amount of

crops produced)

$) The* impro+e con+enience for consumers: e)g) b*

creating foods -ith longer shelf li+es)

D) The* impro+e the nutritional ,ualit* of foods)

E) The* cause less damage to the en+ironment than

con+entional chemicalCintensi+e agriculture)

3.) /hich of the follo-ing is

TR"E regarding most currentl* a+ailable geneticall*

engineered !E< plants0

) Onl* a fe- commerciall* a+ailable processed foods

contain !E plants)

1) There has been -orld-ide acceptance of their use)

$) There are serious biological and en+ironmental issues

regarding their use)

D) #ost !E plants are designed to ha+e better fla+or and

be more nutritious)

33) /hich en4*mes are used to

cut large segments of DN into fragments for DN

fingerprinting0

) re+erse transcriptase 1) DN pol*merase

$) DN ligase D) restriction en4*mes


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35) /h* is it more difficult to

create transgenic animals than transgenic plants0

) nimal cells cannot transcribe and translate foreign

DN)

1) Plants and animals use a different genetic code)

$) It is more difficult to introduce foreign DN into

animal cells)

D) nimal cells cannot replicate foreign DN)

37) !el electrophoresis

separates DN fragments according to their (((((((()

) base se,uence 1) si4e

$) percentage of nucleotides D) electrical charge

38) /h* are bacteria

considered as the -or2horses of genetic engineering0) are al-a*s homo4*gous

1) can readil* ta2e up plasmids containing human genes

and then produce the human proteins encoded b* those

genes

$) the* pro+ide the pol*merase for the pol*merase chain

reaction

D) reproduce +er* slo-l* and accuratel*

39) 1acteria ha+e been

geneticall* modified for each of the follo-ing purposesEX$EPT (((((((()

) to produce bacteria that can clean up to&ic -astes

such as oil spills

1) to produce human hormones including insulin and

er*thropoietin

$) to produce en4*mes used for industrial purposes

D) to produce antibiotics that can be used to treat

bacterial infections

3?) In recombinant DN

technolog*: plasmids ma* be used to (((((((()

) cut DN at a specific location

1) introduce foreign DN into human cells

$) acti+ate restriction en4*mes

D) introduce foreign DN into bacteria

3;) /hich of the follo-ing

techni,ues is similar to a molecular cop* machine for

DN0

) gel electrophoresis

1) pol*merase chain reaction P$R) It is the cells deri+ed from

the embr*os that de+elop from eggs that ha+e beenfertili4ed0

) Embr*onic stem cells 1) stem cells

$) *gote D) pluripotent cells

5.) /hich of the follo-ing

refers to selecti+e breeding techni,ue0

)Is a post transcriptional modification in -hich a single

gene can code for multiple proteins)

1) Is a process to change the genetic ma2eup of cells b*

transfer of genes of interest to another)$) The process of producing similar populations of

geneticall* identical indi+iduals)

D) The process b* -hich humans use to selecti+el*

de+elop particular phenot*pic traits)

53) /hich of the follo-ing

refers to an organism that ha+e been geneticall*

engineered to carr* one or more foreign genes0

) ligated organism 1) transgenic organism

$) homogeneous organism D) mutant

55) The di+iding and

undifferentiated cells are 2no-n as (((((((((((((

) Embr*o 1) Proembr*o

$) $allus D) Primordium

57) ims of plant breeding are

to produce (((((((((((((() earl* maturing +arieties 1) diseaseCfree +arieties

$) highC*ielding +arieties D) all the abo+e

58) /hat is meant b* cloning0

) Production of G!G gene in E) coli)

1) To preser+e the genot*pe of organism

$) To replace the original one

D) all of these

59) /hat happens during

transformation0

) a pro2ar*ote is changed into a eu2ar*ote)

1) a cell ta2es in DN from outside the cell)

$) foreign DN is inserted into a plasmid)

D) the chromosome of a bacterium is mutated)


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5?) /h* are plasmids so

-idel* used in recombinant DN studies0

) because it is difficult to insert ne- genes into them

1) because the* can be used to transform bacteria

$) because the* naturall* contain much foreign DN

D) because the* cannot be cut -ith restriction en4*mes

5;) /h* is inserting plasmids

in *east more comple& than inserting them into bacteria0

) %easts are pro2ar*otes) 1) %easts are h*brids)

$) %easts are eu2ar*otes) D) %easts are inbred)

5=) /hich of the follo-ing is a

pair of transgenic organisms0) a h*bridi4ed peach tree and bacteria that ma2e human

insulin1) a 1t corn plant and a pol*ploid banana tree

$) a h*bridi4ed peach tree and a pol*ploid banana tree

D) bacteria that ma2e human insulin and a 1t corn plant

7>) Doll* is a sheep produced

b* cloning) /hich of the follo-ing is a difference

bet-een Doll* and animals produced b* se&ual

reproduction0

) The source of Doll*Hs DN is a single cell ta2en from

an adult indi+idual)1) The DN molecules in each of Doll*Hs cells are

identical)

$) Doll* has a mi& of genes from her foster mother and

the sheep that donated a nucleus of one of its cells)

D) Doll* is geneticall* identical to her offspring)

7.) !# crops that produce a

higher *ield per plant than unmodified crops -ould

(((((((((((((((((()

) produce more food for the same acreage)1) produce less food for the same acreage)

$) produce more food but -ould use more land)

D) produce less food and re,uire more land)


produces multiple identical copies of a gene for basic

research or for largeCscale production of a gene product0

) restriction en4*mes 1) gene cloning

$) DN ligase D) gel electrophoresis

75) /hich is true of geneticall*

engineered plants0

) are more difficult to engineer than animals)

1) include a transgenic rice plant that can help pre+ent

+itamin deficienc*)

$) are being rapidl* de+eloped: but traditional plant

breeding programs are still the onl* method used to

de+elop ne- plants)

D) are banned throughout the -orld)

77) /hat is 6oined together in

order to produce a recombinant DN molecule0

) one mRN -ith a DN segment

1) one mRN -ith a tRN segment

$) t-o mRN molecules

D) T-o DN segments

78) It refers to a gene produced

for recombinant DN technolog* contains a gene from

one organism 6oined to the regulator* se,uence of

another gene)

) transgene 1) 6un2 gene

$) chimeric gene D) None

79) /hich refers to the group

of geneticall* similar organisms obtained b* a se&ual

reproduction0

) $lone 1) Population

$) ssembl* D) None

7?) /hich of the follo-ing

describes the group of organisms produced b* cloning0

) ase&ual method and geneticall* similar

1) ase&ual method and geneticall* dissimilar

$) se&ual method and geneticall* similarD) se&ual method and geneticall* dissimilar

7;) /hat are transgenic

organisms0

) produced b* recombinant DN technolog*

1) e&tinct organisms

$) naturall* occurring and endemic

D) produced b* traditional plant breeding techni,ue

7=) /hat are the main tools

re,uired for recombinant DN technolog*0

) +ector: desired gene

1) +ector: desired gene: mRN of desired gene: host:

restriction en4*mes: ligases

$) desired gene: host: +ector

D) +ector: desired gene: mRN of desired gene: host

8>) /hat is in+ol+ed in

construction of a recombinant DN0

) clea+ing DN -ith restriction endonuclease and

6oining -ith ligase

1) clea+ing DN -ith ligase and 6oining -ith

endonuclease

$) clea+ing and 6oining DN -ith restriction

endonuclease

D) clea+ing DN -ith restriction endonuclease and

6oining -ith pol*merase


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8.) /hich of the follo-ing

refers to the process of identif*ing genes of interest b*

obser+ing the ph*sical characteristics to find organisms

-ith interesting or useful characteristics0

) re+erse genetics 1) for-ard genetics

$) selecti+e breeding D) cloning


refers to the process of identif*ing genes of interest b*

disrupting the gene of interest and then screen the

resulting modified organisms for changes in the ph*sical

characteristics0

) re+erse genetics 1) for-ard genetics

$) selecti+e breeding D) cloning

85) /hat is the method of

sorting DN fragments according to its si4e0

) electroporation 1) restriction anal*sis

$) electrophoresis D) DN probe


refers to the genes that regulate -hen the gene is

acti+ated or inacti+ated0

) promoter 1) controller

$) mar2er D) +ectors

88) /hen the nucleus is

remo+ed in an egg cell: the result is called(((((((((((((()

) enucleated ooc*te 1) 4*gote

$) embr*o D) ooc*te

89) /hich refers to the method

of obtaining ne- plants from speciali4ed plant parts b*merel* pulling the plant parts apart0) separation 1) di+ision

$) cutting D) grafting

8?) /hat method of plant

cloning ma2es use of plant material from t-o separate

plants 6oined together to form one plant0

) separation 1) di+ision$) cutting D) grafting

8;) /hat method of plant

cloning ma2es use a small amount of tissue remo+ed

from a parental plant to regenerate a ne- plant0

) grafting 1) tissue culture

$) la*ering D) cuttings

8=) /hich of the follo-ing statements about plant

cloning is true0

) Plant cloning is one of the ne-est forms of

biotechnolog*)

1) $loning plants are much harder than cloning animals)

$) $uttings are usuall* onl* ta2en from lea+es)

D) Plants that ha+e seedless fruit must be reproduced

through +egetati+e propagation)

9>) /hich of the follo-ing

statements about animal cloning is FASE0

) $lones can ne+er occur naturall*)

1) The main challenge for scientists at this point is to

ma2e the cloning process easier and more efficient)

$) $loning can produce completel* normal animals)

D) One of the problems -ith selecti+e breeding is that

unfa+orable characteristics can be passed on to offspring

along -ith the fa+orable characteristics)


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Biotechnology Exam