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BioMiNT. Cell Encapsulation for Tissue Engineering. Alok Vij 1 Faculty Mentor: Professor AP Lee 2,3 Graduate Mentor: JS Fisher 2. 1 Department of Materials Science and Engineering, Northwestern University 2 Department of Biomedical Engineering, University of California, Irvine - PowerPoint PPT Presentation
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April 22, 2023 IM-SURE Symposium
BioMiNT
Alok Vij1Faculty Mentor: Professor AP Lee2,3
Graduate Mentor: JS Fisher2
Cell Encapsulation for Tissue Engineering
1 Department of Materials Science and Engineering, Northwestern University2 Department of Biomedical Engineering, University of California, Irvine
3 Department of Mechanical and Aeronautical Engineering, University of California, Irvine
April 22, 2023 IM-SURE Symposium
Agenda
Introduction Materials Methods Results Conclusions
April 22, 2023 IM-SURE Symposium
Applications Tissue Engineering Scaffolds Immunoprotection of Implants Single-cell assays
Cell Encapsulation
Images: www.science.org, www.manager-magazin.de
April 22, 2023 IM-SURE Symposium
Agenda
Introduction Materials Methods Results Conclusions
April 22, 2023 IM-SURE Symposium
PDMS devices Alginate Oleic acid Calcium-containing
polymerizing agent
Materials
Images: www-biol.paisley.ac.uk, www.babonline.org
April 22, 2023 IM-SURE Symposium
Materials
Alginate Biocompatible Non-degradable Gentle gelation Easily manipulated
Images: www.lsbu.ac.uk
April 22, 2023 IM-SURE Symposium
Agenda
Introduction Materials Methods Results Conclusions
April 22, 2023 IM-SURE Symposium
Methods
Device Fabrication Photoresist Expose Develop PDMS cast Anneal
Image modified from http://www.postech.ac.kr
April 22, 2023 IM-SURE Symposium
Methods
Alginate/cell suspension Droplet generation In-channel polymerization Bead isolation Bead harvesting Cell culturing
April 22, 2023 IM-SURE Symposium
Agenda
Introduction Materials Methods Results Conclusions
April 22, 2023 IM-SURE Symposium
Successes: Even distribution of cells Bead polymerization Droplet generation Bead isolation Bead harvesting
Work in progress: Microbead stability Cell viability
Results
Fibroblasts suspended in alginate
April 22, 2023 IM-SURE Symposium
Results
In-channel polymerization
April 22, 2023 IM-SURE Symposium
Results
Droplet Generation Before:
April 22, 2023 IM-SURE Symposium
Results
Droplet Generation Before:
April 22, 2023 IM-SURE Symposium
Results
Bead separation Before:
April 22, 2023 IM-SURE Symposium
Results
Bead Harvesting Before:
April 22, 2023 IM-SURE Symposium
Device Design
Before:
April 22, 2023 IM-SURE Symposium
Device Design
Before:
April 22, 2023 IM-SURE Symposium
Device Design
Before:
April 22, 2023 IM-SURE Symposium
Device Design
Before:
April 22, 2023 IM-SURE Symposium
Device Design
Before:
April 22, 2023 IM-SURE Symposium
Device Design
April 22, 2023 IM-SURE Symposium
Results
Droplet Generation After:
April 22, 2023 IM-SURE Symposium
Results
Bead Separation After:
April 22, 2023 IM-SURE Symposium
Results
Bead Harvesting After:
April 22, 2023 IM-SURE Symposium
Current Work
Bead stability
April 22, 2023 IM-SURE Symposium
Current work
Cell viability assessment
April 22, 2023 IM-SURE Symposium
Agenda
Introduction Materials Methods Results Conclusions
April 22, 2023 IM-SURE Symposium
Conclusions
Success! Next steps:
Integrate with other devices Cell factor delivery Concentration gradient generators Cell sorters Switches
Publish
April 22, 2023 IM-SURE Symposium
Thank you!
Professor Abe Lee Jeff Fisher Said Shokair National Science Foundation
Jeff Fisher
Abe Lee
Said Shokair
April 22, 2023 IM-SURE Symposium
Questions?