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BioLumeBioLume
BioLumeBioLume
PresentationJanuary 10, 2007
BioLumeBioLume
BioLumeBioLume MissionMission
…..to develop and commercialize a broad library of proprietary bioluminescent proteins with multiple first-in-class applications
in diagnostic imaging, food, beverage, and cosmetics…..
BioLumeBioLume
BioLume BioLume Imaging PatentsImaging Patents
• Detection and visualization of neoplastic tissues and other tissues
–U.S. 6,416,960 issued July 9, 2002–U.S. 6,596,257 issued July 22, 2003–U.S. 09/746485 filed December 22, 2000
• Broad claims e.g., “A diagnostic system containing a bioluminescence generating system”• Backed up by broad composition of matter patents
BioLumeBioLume
What is Bioluminescence?What is Bioluminescence?Bioluminescence is light produced by a chemical reaction within a living organism
BioLumeBioLume
Bioluminescent Bioluminescent ProteinsProteins
o
o oc
OX Y GEN
COM P L EX
CO 2
COEL ENT ERA ZINE (Z) ENZY ME"LU CIFERASE"
+ +
+o o
Visible light
BioLumeBioLume
Bioluminescent Bioluminescent ProteinsProteinsBioLume’s bioluminescent proteins were cloned from various deep-sea marine organisms
BioLumeBioLume
Bioluminescent Bioluminescent ProteinsProteinsSardines, Herring, Haddock, Tuna, Salmon, and Mackerel all have diets rich in bioluminescent organisms and contain luciferases and their substrate coelenterazine
BioLumeBioLume
Bioluminescent Bioluminescent ProteinsProteinsFood squid observed during fishery collection
BioLumeBioLume
Bioluminescent Protein Bioluminescent Protein SafetySafety
These proteins have already been tested for skin, eye, and oral toxicity (both acute and sub-acute)
These proteins were negative for mutagenicity in the Ames test.
Many pharmaceutical and university research laboratories have maintained animals expressing Luciferase, with no reports of toxicity or failure of animals or cells to thrive due to their presence.
Coelenterazine is a natural antioxidant
BioLumeBioLume
Molecular ImagingMolecular Imaging
Molecular imaging represents a paradigm shift in the focus of medical imaging from the visualization of anatomy and physiology to the study of metabolic and physiological processes e.g., cancers, often in real time
BioLumeBioLume
Molecular ImagingMolecular ImagingRecent advances in biotechnology have enabled in vivo molecular imaging of luciferase reporter proteins in living animals by using a cooled charge-coupled device (CCD) camera.
BioLumeBioLume
Bioluminescent Bioluminescent ImagingImaging
BioLumeBioLume
Molecular ImagingMolecular Imaging
Because bioluminescence imaging has minimal background activity, this technology is very sensitive for detecting light emitted from luciferases
BioLumeBioLume
Bioluminescent ImagingBioluminescent Imaging
BioLumeBioLume
Bioluminescence Molecular Bioluminescence Molecular Imaging with Imaging with renillarenilla luciferaseluciferase
BioLumeBioLume
Bioluminescent ImagingBioluminescent Imaging
BioLumeBioLume
Bioluminescent ImagingBioluminescent Imaging
BioLumeBioLume
Bioluminescent ImagingBioluminescent Imaging
BioLumeBioLume
Bioluminescent ImagingBioluminescent Imaging
BioLumeBioLume
Skin Cancer FactsSkin Cancer Facts
• >1 million new cases of skin cancer will be diagnosed in the US this year (>10k will die)• 1 in 5 Americans will develop skin cancer during their lifetime (BCC, SCC, or melanoma) • 1 in 65 Americans were likely to develop melanoma during their lifetime• Melanoma incidence has increased 690% since 1950• Melanoma is the second most common cancer in women aged 20 to 29 Source: American Academy of Dermatology
BioLumeBioLume
Skin Cancer DetectionSkin Cancer Detection
• Screening for skin cancer is a visual exam by patient or dermatologist
• Suspicious lesions get a biopsy
• Highly specific antibodies for melanoma are known and generically available
• There are no skin cancer screening kits on the market today
BioLumeBioLume
Immunology of Immunology of MelanomaMelanoma
There are unique surface antigens which There are unique surface antigens which characterize melanoma cellscharacterize melanoma cells
These unique antigens can be used to target These unique antigens can be used to target highly specific antibodies, for therapy or highly specific antibodies, for therapy or detectiondetection
BioLumeBioLume
Immunology of Immunology of MelanomaMelanoma
One type of surface antigen is a ganglioside One type of surface antigen is a ganglioside (GM3)(GM3)
Antibodies have been made which are directed Antibodies have been made which are directed against these gangliosides as well as other against these gangliosides as well as other melanoma surface antigensmelanoma surface antigens
BioLumeBioLume
Selective melanoma Selective melanoma antibody kill melanoma antibody kill melanoma cellscells
BioLumeBioLume
Bioluminescent Bioluminescent Melanoma DetectionMelanoma Detection
Luciferases can be expressed with antibodies to melanoma surface antigen(s)
Or
Luciferases can be chemically conjugated to the melanoma antibodies
BioLumeBioLume
SummarySummary
• Product development opportunity combining known antibodies and developed bioluminescent proteins (no research required)• Patented bioluminescent proteins• Focused on an unmet medical need• Large market opportunity