Bioreactor Design – Fundamentals

The most widely used Industrial Bioreactors are:

•Batch Reactors•Continuous Reactors

•Continuous Reactors With Recycle•Semi Continuous Reactors

(“Fill/Draw” Reactors including Sequencing Batch Reactors)

Bioreactor Design – Fundamentals (Cont’d)DEFINITIONS

“Limiting Substrate”The substrate or substrate(s) (energy source, carbon source and/or nutrient source) which is/are first exhausted in batch growth. This substrate(s) has a direct influence on the kinetics of cell growth.

Bioreactor Design – Fundamentals (Cont’d)

DEFINITIONS“In Excess”

The substrate or substrate(s) (energy source, carbon source

and/or nutrient source) which is/are NOT exhausted at the end of

batch growth. This substrate(s) has NO EFFECT on the kinetics of cell

growth.

Bioreactor Design – Fundamentals (Cont’d)

Bioeactor Design – Fundamentals (Cont’d)

k1* and A are combined to form another constant k and the rate expression

becomes:RATE = k (S / KS + S)

Where k = maximum rate of transport of S ( mol / h)

When S is much larger than KS :RATE ≈ k ≠ f (S)

When S is similar to KS :RATE = f (S)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

THE LAG PHASE

Bioreactor Design – Fundamentals (Cont’d)

THE LAG PHASE

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design – Fundamentals (Cont’d)

Bioreactor Design Fundamentals

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)

Bioreactor Design Fundamentals (Cont’d)