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BIBLIOGRAPHY
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1~7
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Blpln Blharl P.G. College, Jhansi·INDIA
•
fLORA AND FAUNA
iOOSVol 11 No 2 PP 195-196 ISSN 0971 -6920
sTUDIES ON AEROMYCOFLORA OF DAIRY AREA AT RAIPUR (C. G.) INDIA
K.L. TIWARI, S. K. JADHAV• AND S.R. KUNJAM
School of Biotechnology &
•school of Life Science,
PI Ravi Shankar Shukla University, RAIPUR (C.G.) INDIA
ABSTRACT Aeromycoflora over dairy freld at Rarpur was observed by usmg gravity petnplates methods dunng June 2004
10 september 2004 for rarny season. Dunng mvestrgation penod 261 colonres of 19 fungal species were observed. The axrmum number of fungal specres (14 species of 97 colonies) were observed in the month of August 2004 while
mrmmum number of fungal species ( 11 specres of 49 colon res) rn the month of June 2004 The maxrmum percentage ~ontnbutron of Aspergillus mger (14.17%) was followed by Penictfllum sp. (12 26%) Curvulana sp (10.72%) and ~spergilfus fum1gatus (9 19%).
Table 01 F1gure: 00 References : 14
KEY WORDS: Aeromycoflora, Frequency, Contnbutlon
Introduction Vanous m1croorgamsms were present 1n the
ir of anrmal house which affect the health of rnrmals as well as the people those workrng there. rgh concentration of fungal spores plays an
'ioportant role rn dermatophytes deep mycosis and laeroallergens in the animal house
Materials and Methods The survey was carried out for four tnonths
~om rainy season at dairy area intramural of Raipur. luring rnvestigatron periods gravity petriplates fnlaining PDA (Potato Dextrose Agar) medi8 were :>posed fortnrghtly for 5-10 minutes rn the ilmosphere of dairy. The exposed petriplates were 1rought into the laboratory and incubated at 26 :': 1 "C ~seven days 1\t the end ofth~ incubation period the ~rcentage ccntnbution and percentage frequency ~~ere assessed 11 The tdentificaticn of fungal spores ~s done with the help of available literatures'''
Results and Discussion Dunng mvest1gat1on period 261 colon1es of
19 fungal specres were observed (Table-/) Among he total fungal flora 14 species belonged to Class leuteromycotrna (73 68%), 2 specres formed :lass Zygomycotrna (1 0 52%), 2 specres were from rniden!lfted groups (10 52%) and 1 from Mycelia lenlra (5.26%) of the total arr spora
The max1mum percentage contnbut1on of
Aspergillus niger ( 14 17%) was followed by Pemcrlfium sp (12.26%), Curvularia sp. (10.72%), Aspergillus fumigatus (9.19%), Alternaria sp. (7.66%) and Rhizopus sp. (6.51 %). The maximum number of fungal specre~ (14 species of 97 colonies) were recorded in'the month of August 2004 while minimum number (11 species of 41 colonies) were observed in the month of June 2004.
Some fungal species were most frequent during investigations i.e A.. niger, A. nidulans, Cu(llularia sp. and Mucor sp. While Cladosporium sp., Nigrospora sp. and unidentified sp. I & II were least frequent.
The results of present investigations showed similarities to the indoor studies conducted by other rnvestigators. In library the major types of mycoflora reported were as Aspergillus, Cladosporium, Nigrospora and Altemana" Higher concentration of Aspergillus was recorded rn buffalo shed" .. Cladosporium, Fusarium and A.spergrl/us with major concentration in cattle shed was recorded' A worker recorded the maJOr concentration of Cladosporium, Aspergillus, Curvulana and Rhizopus over poultry shed'. Hrgher concentration of Aspergillus, Curvularia and Nigrospra in chemistry lab, that of Aspergillus and Curvularia in green house and of Aspergillus in Balodabazar. Cladosporium and Curvularia in inside and outside the caves was recorded'.
I
II
K.L Tiwari, S.K. Jadhav and S.R. Kunjam School of Studies in Biotechnology
Pt. Ravishankar Shukla University, Raipur (C.G.). 492010
1\eromyconora ovc:1 :.lum area at Ra1pur {Kukurbeda) was canied out from June 2004 to December 2004 with th. rav!(y petnplatcs method Dunng present 1nvesttgat10n 435 colonieS of 28 fungal species were 1denufied. Maxi ;pectes fungal spec1es were observed 1n the month of November While nunimum 10 fungal spcc1es were observet :. Out ot total fungal flora. !he percentage contnbutmns of different classes were as follows. Zygomycotma { 7 14'/'r· 1 wcotma (67 85%), umdent•fied groups (21.42%) and Mycelia sterilia {3 57%) Out of total fungal populatJOI us m~:er(l2 87%} was mosl dommaled whereas. Cun'u/arw sp (I! 72~). CJadmprmJJm sp (9 88'k>. A!unwnr lb) and Pernn!IJUm sp (6 66~) were codorrunant fungal spores
· A.eromycoflora, Dominant. Slum area.
II · . i / fl.• !J•tu•allcnal, "Bia•••al '7<-ma,dt /}••tnal7J•6tu~.~ ••~" U.t a••plus •6 j~u.~.,.r -6 ]JlA•tS&JuUJ, .O.~la} i I This publication is included in the abstracting and indexing coverage ofMediciool and Aromatic
I I i I
, i
Plant Abstracts, Indian Science Abstracts C.A.B. International Horticultural Abstract, C.A.B. International Plant Pathology Abstract, Paryavaran Abstract, Bio-Science Information Service (Biological Abstracts), Current Indian Forestry Environment and Wild life Abstracts and others.
, Editor-in-chief:
Dr. S.K. Gupta M.Sc .. Ph.D
Retd. Head, Department of Botany DAV. College,
Muzaflarnagar-251001, U.P. India
Mailing Address:
Chief Editor, Advances in Plant Sciences
657/6 (770), South C1vil Lines, Muzaflarnagar- 251001.
U.P India !lJ(0131)·2621276
l i - --
ISSN 0970-3586 Ad. Plant Sc1. 19 (II) 387 - 390 , 2006
~ Aeromycoflora of Slum area of Raipur ~ (C.G.) India
K.L. Tiwari, S.K. Jadhav and S.R. Kunjam School of Studies in Biotechnology
Pt. Ravishankar Shukla University, Raipur (C. G.). 492010
Aeromycoflora over .!>lum area. ar Ra1pur (Kukurbeda) was carried out from June 2004 to December 2004 with the help of gravity petnplates method Dunng presenl mvest1gauon 4~5 colome.~o of 28 fungal specu:~s were Jdl!nufied Maximum 17 species fungal spec1es were observed in the moarh of November while nummum 10 fungal spcc1e.., were observed tn August Our of total fungal flora. the percentage contributions of different classes were as follov. ~. Z)gom)COIJna (7. 141/f.)
Dcuteromycotina (67 85%), umdenufied groups (21 42%) and Mycelia srerilia (3 57%) Out of rota! fungal population Aspergillus mger (12 87%) was most dommated whereas. Cun·ularw sp (II 72%), Cladosportum sp (9 884). A/le/JWrtll sp (9 \9%) and Peninllwm sp (6 66%) were codommant fungal spores_
Keywords : Aeromycoflora. Donunant. Slum area
INTRODUCTION
Microorganisms (fungi, bacteria and actinomycetes etc.) are present in the atmosphere. The populalion of microbes and other biological particles in the atmosphere is referred as airspora (Gregory, 1961 ). Some airborne fungal spores are known to be responsible for the diverse allergic disorder. The present work provides a critical account of the airbone fungal spores of slum area at Raipur (Kukurbeda) dealing with their numerical and monthly variations in the air.
MATERIALS AND METHODS
Fungal spores occurring in the atmosphere of slum area at Raipur were trapped during June 2004 to Decmber 2004 at 15 days intervals with the help of gravi~ PetJiplates method. Five petriplates containing potato dextrose agar (PDA) medium were exposed in the air for 5-10 minutes at the five different places of slum area of Raipur. The exposed petriplates brought to the laboratory and incubated at 26 ± 1•c for 5-6 days. After incubation period, fungal colonie• were counted. isolated and identified on the basis of available literatures (Barnett. 1969, and Ellis, 1949, etc.)
RESULTS AND DISCUSSION
During present study 28 fungal species of 435 colonies were recorded. Monthly variation and percentage contributions of the fungal species were calculated (table-1 ). Out of total airspora Zygomycolina (7.14%), Deuteromycotina (67.85%), unidentified groups (21.42%) and Mycelia sterilia (3 57%) were recorded. Deutennycotona were highest in number.
During this study Aspergillus niger. Memana sp., Curvularia sp. and Drechslera sp. were
Advances 1n Plant Sciences December 2006 387
Aeromycoflora ol Slum area of Ha1pur (C G ) lnd1a
must frequent fungi, whereas Aspergillus fum1gatus. CladoSponum sp . Pemciil1um sp., Aspergillus lerreus, A Juchensis, Fusanum sp and Mucor sp were moderate frequent fung1 On the contrary Botryodiplodia sp., Torula sp., Aspergillus sp I, Alternaria brassicola and unidentified sp. showed least frequent fungi. Tiwari and Sahu (1991 ), Sharma (2001) and Jadhav and TIWari (1994) have also reported Curvularia, Alternaria, Cladosporium and Aspergillus were most frequent.
The results also indicate that maximum percentage contribution of Aspergillus niger (12.87%), Curvularia sp. (11.72%), Cladosporium sp. (9.88%), Alternaria sp. (9.19%) and sterile mycelia (8.73%) and minimum percentage contribution of Penicil/wm sp. (6.66%), Aspergillus terreus (6.45%), Drechslera sp. (6.20%), Aspergillus lumigatus (5.51%) and Fusarium sp. (4.13%)
Maximum numbers of fungal species (17) were reported in the month of November while min1mum number of fur.gal species (10) in the month of August.
Asporgillus niger. Altemana sp., Curvularia sp and Drechslera sp. were present throughout the month. Certain fungi were present more then one month but not 1n all months like Aspergillus mdulans, A fumigatus, A /uchensis, A terreus, Cladosporium sp , Penicillium sp., Fusarium sp., Mucor sp., He/minthosporium sp and Ntgrospora sp.
Some fungi were present only in particular month like Aspergtllus sp. I and unidentified sp. II present only in June. BotryodiplodJa sp and un1dentified sp. Ill were present in ttie month of June and July Alternaria brassicola was present only in October and Btspora sp. was present in the month of November, Unidentified sp. IV and V were present only in t',ugust
Aspergillus sp. being the most common genus occupied the first position Aspergillus sp. w2s found to be most predominant in the air at Raipur, (Tiwari and Sahu, 1988 and Sahu, 1998). Highest concentration of Aspergillus sp. was also observed -From differe~t places, Aurangabad (nlak and Slinivasulu, 1967), Kanpur (Rajan el. a/, 1952) and Raipur (liwari and Jadhav. 2004, Tiwari and Tiwari. 2005). During monthly observation it was also observed that total ~Jngal species increase from September to Dec:ember month. Simtlar results were also reported by Maribhatt and Rajasab (1991) from Gulberga, Uddin (2005) from West Bengal, Pund and Trdke (2005) from Amravati and Jadhav (1996) from Raipur. Similarresults also obtain by Sharma (2001) and Sahu (1996).
During investigation period 1t was observed that the atmosphere of slum area at Raipur (Kukuroed;;1) was never free from 'fungal spores throughout the month.
REFERENCES
Barnett, H.l. 1969. fffustrated Genera of Imperfect Fung1 Burgess Pub Co Mw,neapo(•s. Mtnnesota. Ellis, M.B. 1949. Demitiaceous Hyphomycetes Common Wealth Myrologicallnsbtute, London. GregorJ, P.H.1961. The microbloi()Jy of the atmosphere. Leonared HtiiBC>Ok Ltd New York (2nd Ed.) Jadhav, S.K. 1996. Studies of Aeromycoflora over R•ce field at Balodabazar, Rarpur Ph 0 Thests. Pt RaVIshankar
Shukla University, Raipur Jadhav, S.K. and Tiwari, K.L. 1994. Aeromycoflora of Ravan vrllage. Indian Bot Reprt 1 (1&2}: p. 33-36. Maribhatt, M. and Rajasab, A.M. 1991. lnadence of arrbome fungal spores at tv.to different srtes in Gulberga
dunng 1984-86. Indian J Aerobiology 4 (1&2): p-1-6 Pund, Smita, B. and Tidke, J.A. 2005. Pnlrmrnary observation on some qualrtatrve anarysrs of ariborne
brocomponents at Amravatr Abst 13th Nat Conf Aerob1ology /nst1tute of Soen~. Nagpur.19 P-14 RaJan, B.S.V., Higam, S.A and Shukla, R.K 1952. A study of the atmosphenc fungal flora of Kanpur, Proc lnd
Acad Sa. 35 : 33- 37
AUvdfiGes 1n Plant Sc1ences December 2006 388
K L T1wan e/ a/
Table 1 :Showing monthly spore variatio.n-5, percentage contrit:,uti~n.and percentage frequency · of airbor.ne fungal species ove_r's'lun\ _ar~a, Ralpur from June 2004·to December 2004
S. ,.I Fungal spores June July Aug. Sep: Oct. Nov. Dec :Total No . Percentage Percent· No. · . · btFunga I frequency age Con-
colonies tribution
1. Aspergillus niger 02 06 16 05 08 10 . 09 56 100% 12.87% -
2. Aspergillus luchensis 06 Of . 01 . . 02 1,0 57.14% 02.29% -
3 Aspergillus nidulans o-z· - . '03 . 114' 'b3 12 51.14% 02.75%
4. Aspergillus fumigatus . 02 05· 04 03 .. . 05 05 '24 84.71% 05.51% 1-·-
5. Aspergillus terreus
. 1.0+02
. 11 07 06 02 28 71.42% 0643%
6: Aspergillus sp. I . . . . . 10 14 28% 02.29% . .. .. . . .. . . .. . .. ... . - -
7 Affemaria brass1cola - . 01 - '01 ' ·14 28% 0.22% - '
8 Affemaria sp. 03 07 09 07 04 .·. 07· .• 03 AD \' 1 100% 09.19% f.-
9. Bispora sp. - . . - • - 02 - 02 14.28% 0.45% "
io. •
Botryodiplodia ;;p. 01 01 - .. - - 02 . 28.57% 0.45%
11. Cladosporium sp. - 01 06 03 08 15 10 43 85.71% 09.88%
12. Curvu/aria sp. 06 05 11 05 12 05 07 51 100% 11.72%
13. DreG~~&tera 8p. .~ •04 . , Ol!' ,a$ Q2 Q3 00 27 100% 06.20%
14. f:usariwil sp . - 00 07 03 '0'2 1a 5i 14% 04.i:l%
15. Helminthosporium sp. - - . - 02 . .. 05 03 10 42.85% 02.29% -.
16. Mucorsp 01 I 02 - - 03 . 02 . oa .-. ' 57.14% 01.14%
17. Nigrospora sp 03"· ·a1 01 ' 0"' . 42.85% ~1.14% - . . ,)_ h
18. Paece/lomyces ~P . . . . . 03 02 05 28.57% Ot\4'4• . '
19. Penicillium sp 03 . 08 . '0,6 . 08 04 . 29 7142% 06.66%
20 -~-- -Rhizopus sp 0" 02 03 . . - . 07 42.85% 01.60%
"'" 21. Torula sp - . . - - 0~ 01 '02' 28.57% 0.45%
22 Umdentmed sp I . . . 01 - . . ,01. 14.28% 0.22% -23. Unidentified 5p. II 01
.• ·. . -01 . 14.28% 0.22% . . .
24. Unidentified sp. Ill 01 01 . " . . . .. . 02 • 2857% 0.45%
25 Unidenflfied sp IV - 01 . - ., - - 01 .. . 14.28% 0.22%
26 Umdent1fted sp V - 01 . . - - 01 ' 14.28% 022%
27 Umdenflfied sp VI - - 01 . - 01 14.28% 022%
28 Mycelia stenlla - 08' 05 . 05 Hl 07 ~ 38 65.71% 08 73%
Advances 1n Plant Sc1ences December 2006 389
Aerornycoflora of Slum area of Rarpur (C G ) lndra
Sahu, K. 1998. Aeromycological studies over wheat crop plants at Ra1pur (M P.). Ph.D Thesis, Pt Rav1shankar
Shukla Un1vers1ty, Raipur .1•• ,
Sahu, S.K. 1996. Stud1es of Aeromycof\ora associated 'With SpiriaCh Ph.D Thesis·. Pt. Ravish'ankar Shukla
University, Raipur. Sharma. K. 2001. Studies of aeromycoflora in relation to leaf surface mycoflora of Ocimum sant"lum 1.., Ph.D.
Thesis, Pt. Ravishankar Shukla University, Raipur. · · Tllak, S.T. and Srinivasulu, B.V. 1967. Airspora of Aurangabad. Ind. J. Microbiol. 7: 167- 170. . , ... Tiwari, K.L and Sahu, S. 1988. Studies on the ieaf surface and air mycoflora of Momordica charantia. L. Plant.
Geobrose new reports 7 : 135 - 139. Tiwari, K.L. and Jadhav, S.K. 2004. Aeromycological studies of chemistrY lab of Govt. College, Balodabazar,
Raipur (C.G.) India. Ecol. Enb. & Cons. 10 {3): pp- 383- 385.. , Tiwari, P. and Tiwari, K.l. 2005. Aeromycoflora of Pachpedi Naka, Raipur. Abst. 13th Nat. Conf. Aerobiology.
Institute of Science. Nagpur. p-45. Tiwari, K.L and Sahu, S.K. '.1991. Studies on the leaf surface mycoflora of Pisum sativum l. in relation to age
and environmental factors. Ad. Planl Sci. 4(1): 141 - 149. · '' · Uddin, N. 2005. Estrmation of aeromycoflora of Jute field at West Bengal India. Aerobiologia 21 : p. 345. 349
(Received on 8 June, 2005; Accepted on 29 .Augu~t. 2005)
Advances fn Plant Sciences December 2006 390
810/NFOLET 3 (2) 142-143. 2006
SEWAGE FUNGI OF RAIPUR CITY
K. L TIWari •, S. K. Jadhav~ and s. R. Kunjam
'SOS in Biotechnology, Pt. Ravishankar Shukla University, Raipur (C. G.) 492 010. "SOS in Life Sciences. Pt. Ravishankar Shukla University, Raipur (bG.) 492 010
. . '
Sewage water contains wastes from residence, business and industnes These wastes, are offensrve and support the growth of harmful bact en a and other microorganrsms. The present paper deals with monthly variation rri sewage fungi of Rarpur Crty.
The survey was earned out dunng July to November 2004 at 15 days intervals by using pour plate method The sewage water was collected from five places of Raipur c1ty i e Budatalab, Kankalrtalab, Gudhiary, RaJa!alab and Amanaka, poured in Petri plates containing PDA (Potato Dextrose Agar) medrum and incubated at 25 2: 1 'C for 5-6 days. After the incubation period fungal colonies were counted, isolated and Identified on the basis of available literatures (Clements and Shear, 1931, Ell1s. 1949, Barnett, 1969).
r '1
. o_u'ring_ p,lesent study 301 fungal colonies of 19 fungal species were recorded (Table 1 ). Out of the total fungal flora. percentage contribution of Zygomycotina was 15.78%, Ascomycotina was 5 26% while that of Deuteromycotina it was 78 94% Species belongs to Deuteromycotina were highest in number while those from Basidromycotina were totally absent Maximum numbers of fungal spores (14) were isolated during November, moderate ( 12) during September while minimum (08) species were isolated during the month of August.
It was observed that Aspergillus nigar (30.56) Aspergillus fumigatus (22.92%) Penicillum sp. (15.28%) Paecilomyces sp. (8.63%) Aspergillus phoensis (8.63%) and Curvu/aria sp. (2.32%) were most abundant,
Table 1 : Monthly spore variations and percentage contribution of sewage fungi at Raipur city from July 2004 to November 2004
Total No Percentage Fungi July Aug. Sep Oct. Nov. of fungal contribution
colonies
Aspergillus fum1gatus 07 12 27 13 10 69 22.92
Aspergillus phoens1s 16 01 03 06 26 8.63
Aspergillus nidutans 01 02 01 02 06 1.99
Aspergillus mger 13 20 16 20 23 92 30.56
Aspergillus sulphuns 01 - - 01 033
Asoergillus terreus 01 - 01 01- ~- 03 -- - 0 99
01 ' 03 . ' ',' 099 81spora sp 01 01 - ~·-
L~""eatonllurn ~~, til 01 02 0.66
Vol. 3 (2), 2006 143
; oc!: , I \ : , ~otal No,, ~ percentage
Fungi July At•g. ;: .Sep. . -Nov.· . offungal · contribution
Cladosporium sp. 01 - -
Curvu/aria sp. 02 02 01
Diplodia sp. - - 01 '
Epicocum sp. - - 01
Fusarium sp. 01 - 02
Mucorsp. 01 - -Paecilomyces sp. 15 - 02
Penicillium sp. 08 15 01
Rhizopus sp. 01 - 01
Syncephalastrum sp. - ,- ~'.- ' ' '02
Torula sp. - 01 -
while Aspergillus nidulans (1.99%), Fusarium sp. (1.32%) Cladosponum sp. (0.99%), Mucor sp. (0.66 %) and Aspergillus sulphuris (0.33%) etc. were least abundant fungi. The most f;euent lungi were Apergillus fumigatus, ·A niger, Penicilliumsp., Aspergillus nidulans (80%) and Curvularia sp. (80%) etc. while Aspergillus te!Teus (60%), Bispora sp. (60%), Fusarium sp.
... ~ . colonies.1
' ' ' ' ·~
- 02 03 0.99
- 02 07 2.32
' • I 01 02 0.66 - ,, - 01 02 0.66
01 - 04 1.32
- 01 02 0.66
05 04 26 8.63
14 08 46 15.28
- - 02 0.6
- - 02 0.66
- 02 03 0.99
Cladosporium sp. and Mucor sp. were present in the months of July and November while Torula sp. was present in the month of August and November. Certain fungi were restricted to particular month; Aspergillus sulphuris was present in the month of July while Synephalastrum sp. in the month of September.
{60%) , Cheatomium (40%), Cladosporium sp. References : (40%) and Aspergillus sulphuris (20%) and • Syncephalastrum sp. {10%) were last frequent ;·Barn~~: H. K. (1969) Illustrated genera of fungi. . ? ,. · · .. 'l"}portant fungl." M1ns Burgess Pub. Co.
Some fungal species were preseni.,. Clements. F. E. and C. L;; Shear (1931) "The through! out the year such as Aspergillus. · . genera of fungl. H1gher publlshmg fumigatus, A.niger and Penicillium sp., on the·. · · · company.
otherhand8isporasp.waspresentinthemonth Ellis, M B. (1949) "Demitiaceous of August, October and November, Cheatomium Hyphomycetes", Kew England Common sp., Diplodia sp. and Epicocum sp. were present , Wealth Mycological Institute. in the months of September and November:
-.·- ... ! -
'' ',-. '
- - , ·- - - -r· '
National Symposi'um pn Biodiversity: ·· current status and Prospects
• ' • I
" .... ·-:'
October 17~18, 20os:· .· . . . I
. i
Abstr~ietj~~- :·~·-~ ·r. , . .
, ••. > ••
;.f"'_1::~1 .::
. ' :
' · School of Life Sci~rices · .. Pt. Ravishankar Shukla University
Raipur-492010
c,
lf./ational Symposwm on Biodiversity· Current Status and Prospects, Ratpur 2005
OP-24
Antibacterial properties of some medicinal plants against tooth bacteria
Jadhav S.K, KWljam S.R. and Dewangan D. School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur- 492 0 I 0
Different plant species are used as tooth sticks. During present investigation five traditional medicinal
plants, which are commonly used for tooth stJcks, i.e. Achyranthes aspera L. (Amarantaceae ), Bar/en a
priontis L. (Acanthaceae), Pongamia pinna/a L. (Fabaceae), Psidium guajava L. (Myrtaceae) and
Azadiracta indica Juss. (Meliaceae) ·were selected.
In present studies, isolate the bacteria from the sample collected from the Govt. Dental College, Raipur
(CG.) and cultured. This bacterial culture poured into the extract of the experimental plants then incubated
for incubation period for their growth.
After the incubation period, observed the bacterial growth by colonial growth and measurements of
optical density (OD) in liquid medium. It was observed that experimental plants show antibacterial activity.
It was also observed that different plant shows the different antibacterial properties. The result also
indicates that Azadiracta indica have ma,ximum antibacterial properties. In other plants less growth was
observed in Psidium guajava, Achyranthes aspera, and Barleria priontis in comparison to Azadirachta
indica.
45
4"' CIJHATTISGARH YOUNG SCIENTtSfS CONGIIESS ____ l!_iAI_~_!~JP~J __ :_x_; v:~r~,~n ,,; ',J.1J\l1.'i11Jr.
Wood eta/ (I ·J~X). Total11ater soluble protem and TCI.\ preciprtated protem11as esumated follo\\rng Loll'[\ et a/ (I '!51). quantitatrve estimation of chloroph,·JJ was made follo111n~ Arnon (I '!-l'J) 11 hile caroteno1ds 11 ere determmed follo11 ing Duxbul"l and Yentsh ( 1'!56 ). -50ppm I BA treated cu!!ings sho\\'ed best results m 1 egetati1·e gro11 th as plant he1gh1. number or lem es. number of node and inter node <md number of branches. rooting response as number ol· root; and pnmafl melabolnes as total sugar. reducmg sugar. !Otal soluble protem and TCA prec1pnated protem Root length 11as maxrmum 1\lth I OOppm IBA Chloroph,·ll content 11·as ma~11num 1n lllppm lA A treated cuttings
IV- 6 BIODIVERSITY OF SOIL FUNGI OF TEAK (Ter:tona grandis L.) FIELD II\; MOI-IAL\., RAJN ANDGAON DISTRICT
Shrinnn h:unjam .'.'('hoof n/.\'t11,h~·~ ''!! 1/e ,\'ol'/Ut'\, Ft Rad.\hnnlmr S/mk/11 I nnerlff\', /?(lipur (( · G.J- ../9]()/{J
S01l IS a great resen air of microorganisms Normallv all types of microorgamsms ill e m the soil. !he important ones bemg fungi. bacteria. actmo:m cetes. algae and protozoan. The most 1mpoctant role of'' Yanetl of soil orgamsms IS that the~ act upon dead organic matter. changmg 11 to humus and llnalil ·,o mmeral. 11 h1ch are returned to the soil. The sod sampb ''-ere collected month!}· dunng October 2005 to l<UJUal')· 200/) from different spots 01 Teal-. plant 1 /L, w11a gran.its L . F amih· Verbenaceae) lield with the help of stenle pol\ thene bags fwm sampled so1l The fung1 11ere Isolated \\llh help of Warcups soil plate method i,Warcup. 1'150) on Potato De\ I rose Agar ( PDA) medium and 2Xx I" C for mcubatron period Durmg present studies II)(, colomes of I X fungal specres 11ere recorded. Out of 11hic~ max1mum percenta~c cc>nlnfn,tron or Altcmana sp ( 13 ](I %) and Cladosporium sp (II 32'Yo) follr:med b1 A.,;>crglilll' (unugatuv (Ill :n %) and Asperg;tiJ,, 111gcr ('! 43 %). It was also obsened that so111e species occu1 throughout the .:;easun 1 e A/renwna sp . . ·hflt:Jg!llus niger. Aspergillus twmgarus. C!atlosporulln sp. ( ·un'lu.rrto.~ sp and J-'cruct!lnun sp ere
1\'- 7 PROCTI\ ITY ESTIMATIONS OF AQUATIC MACROPHYTES 1:\ DliRGBHIL\1 CITY IN CHHATTISGARH.
Salish K Sen /I('J'ff ,,, ·;,, :1,, .,.,J,u \{Ill ,''l'omUfl/111 ('ollege. Hlufor
\bioll\\ ,·,( th~ pc>nds ol· Durg-BhiaiJ Cit\ ar~ bin\lcalll disturbed shallo11 and perr,nmal fresh naler hPdic' llh>e arc nch 1n plant dllersJtl ;h''""'g Ju,uriant gro\\1h of' macropl11tes Certain m.Krt'ph<.1c'• '" thc ponds arc found usel'ui I•' nun and domestic ammals Smce macr,•ph,t,·s arc the m,t1or (1'!1\ril'ul,'r~ of pond product!\ 11' 'ht: pre..;t'nlHHesogauons ha\·e been fo('U5~d on th~ same
14TH NATIONAL CONf.ERENCl: ON
AEROBIOLOGY 15th. 171h January .2007
ABSTRACTS
Organised by
SCHOOL OF STUDIES IN BIOTECHNOLOGY Pt. Ravishankar Shukla University
Raipur,Chhattisgarh-492010,India
E-34
SOIL MYCOFLORA OF SARGUJA REGION
Tiwari, K. L., Jadhav, S.K., and Dewangan, A.
School of Stud1es 111 Biotechnology
Pt. Rav1shankar Shukla University. Ra1pur (C G. I - 492010
Soll1s an ol1gotroph1c med1um for the growth of fung1 and also the source of the air mycoflora These
fung1 play an Important role m so1l. degrad1ng the complex sources of organic carbon They convert hard-to
digest orgamc matenal 1nto forms that other organisms can use The different phys1cal and chem1cal
compos1t1on of so11 favors different fung1 The present mvestigation was earned out to observe the fungal
b10d1vers1ty m forest so11 of Sarguja reg1on w1th the help of serial dilution method and PDA med1um Dunng
present mvest1gat1on 333 colonies of 28 fungal spec1es were observed Among these Aspergillus 111ger and
P<e!JICIIilllm rubrum are most frequent spec1es followed by Mucor racemosum and Cladosponum sp The
percentage contribut1on of Aspergillus mger(27 02%) IS h1ghest followed by Pemcillium rubrum t14 4%) and
Muco1 racemosum wh1le Absidia sp N!grospora sp Beltramella sp and Orecflslera sp. (each 0 3% I sllowmg
the lowest percentage contnbut1on
E-35
SOIL FLORA: SOURCE OF AIR FLORA
Tiwari, K.L., Jadhav, S.K., Kunjam, S. and Shukla, S.
School of Stud1es m 81otechnol:>gy
Pt Rav1shankar Shukla Un1vecs1ty. Ra1pur (C G) - 492010 lnd1a
Soil ,_,one of the Important sources of a1r flora and 1s a natural med1um tor growth and reproduction
Tile d1vers1ty of fungal spec1es 1n soil reflects the vanat1on 111 phys1cal and cl1em1cal cond1t1ons of soil as
well as a1r flora In present study, fungal d1vers1ty 111 forest so11 of Ra1nandgaon distnct was earned out
from Apnl 2006 to November 2006 w1th t11e help of Warcups SOli plate method usmg Potato Dextrose Agar
1PDA1 med1um Dunng present mvest1gat1on 218 fungal colomes of 24 fungal spec1es 117 genera) were
1dent1f1ed Maxunum 33 fungal colomes were observed m the month of July and November wh1le m1mmum
19 fungal colomes were observed 1n September Out of total fungal flora, the percentage contnbut1ons
of different classes were as follows Zygomycot1na (8 33%). Ascomycotma (4 16%). Deuteromycotma
179 16'X,I and other groups 18 33%1 mclud1ng umdentif1ed and stenle mycelia Out of·total fungal population
rilteuwua sp 110 og·v,,l Aspe1gillus mge1 19 63%1 and Cladospouum sp (9 63%) was most dom1nated
wl1ereas Pemoltwm sp I 16 88''li·l and Fusarwm sp I 15 04%1 was co-dominating fungal spores
50
ABSTRACTS
51h CHHATTISGARH YOUNG SCIENTIST CONGRESS
(February 28- March 1, 2007)
Sponsored by
CHHATTISGARH COUNCIL OF SCIENCE AND TECHNOLOGY, RAIPUR
Orgnised By
CHHATTISGARH SWAMI VIVEKANAND TECHNICAL UNIVERSITY, BHILAI
r------- FIFTH CHHATTISGARG YOUNG SCIENTIST CONGRESS --------..
13. BIODIVERSITY OF ASPERGILLUS IN THE ENVIRONMENT OF RAIPUR
Shriram Kunjam School of Studies in Biotechnology
Pt. Ravishankar Shukla University, Raipur (C.G.) - 492010
ABSTRACT
Fungal flora differs fiom season to season, month to month and place
to place due to environmental condition. Biodiversity of Aspergillus in the
environment of Raipur was assessed from 2005 to 2006 with help of gravity
petriplates method cont~ining Potato Dextrose Agar Medium.
During present investigation, total 22 Aspergillus species were observed
during the year 2005 and 2006. Out of which, 13 species were observed in
2005 and 20 species in 2006. The percentage contribution of Aspergillus f/avus
was 3.53% in 2005 while 7.84% in 2006. It was also observed that Aspergillus
fumigatus was 1.53% it' 2005 while 6.68% in 2006 and the percentage
contribution of Aspergillus niger was 9.12% in 2005 while 6.55% in 2006.
Keywords: Biodiversity, Environment, Aspergillus sp.
19
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