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B. Insect/machine combinations
C. Synthetic Transplants
D. Animal Surrogate Transplants
E. Engineered Plants/Animals
Types of Biotechnology Applications
1. Blue Biotechnology
- Marine and aquatic applications
- rare
2. Green Biotechnology
-agricultural applications-micropropagation-transgenic plants (Round-up ready beans, Bt Corn)
3. Red Biotechnology
- Medical applicationsEx. Antibiotics, genetic manipulations
4. White Biotechnology
- Industrial applicationsEx. Catalysts and enzymes
Review of the Cell and Chemistry
Basic Organic Chemistry
Monomer: a subunit of a larger macromolecue
Polymer: a large macromolecule made of monomers
4 Most Important Biological Polymers
1. Lipids: greasy compounds that do not dissolve in water
Monomer: fatty acidsImportant Lipids:1. Steroids2. Phospholipids: cell
membrane
2. Carbohydrates: twice the hydrogen atoms as oxygen
Monomer: monosaccharides: simple sugars (C6H12O6)
Disaccharides: 12 carbon, double sugars
Polysaccharides: chain of monosaccharides (cellulose; starch)
3. Proteins: polymer of many amino acids
4. Nucleic Acids: - Monomers: - Large macromolecules
found in the nucleus of cells- Contain genetic information- Deoxyribonucleic acid
(DNA)- Ribonucleic Acid (RNA)- Four bases: Adenine (A),
thymine (T), cytosine (C), Guanine (G)
Cell Theory – 3 postulates
1. All cells come from pre-existing cells2. Cells are the basic unit of life3. All living organisms are composed of cells
Two Types of Cells
1. Prokaryotic Cells: small simple cells-lack a nucleus-DNA called chromatin
2. Eukaryotic Cells: complex cells-membrane bound nucleus-DNA in nucleus-complex organelles
Cell Organelles: Cell’s “organs”
1. Cell Membrane:
FX: regulates what enters/leaves the cell
-phospholipid bi-layer-
2. Nucleus:
FX: 1. store DNA 2. control center of the cell
3. Cytoplasm: gel like substance in the cell
FX: site of all cell activities
4. Endoplasmic Reticulum
Two Types1. Rough ER: makes and
transport proteins (has ribosomes)
2. Smooth ER: transport proteins, makes steroids
5. Ribosomes: small organelles
FX: produce proteins-500,000 per cell
6. Mitochondria: rod shaped; double membrane
FX: Produce energy molecules – adenosine triphosphate (ATP)
“Powerhouse” of the cell
7. Golgi Apparatus: flat sacs
FX: Package, sort, and transport proteins
8. Lysosome:
FX: to break down foreign contaminants
Filled with enzymes
9. Microtubules
FX: support the cell shape
Cytoskeleton
10. Centrioles: Cylindrical structures
FX: assist in cell division
Other Organelles
-Chloroplast: used for capturing sunlight for photosynthesis
-Cilia: small hair like structures used for movement
-Flagella: “tail” used for locomotion
Cellular Respiration
-set of metabolic reactions-Produces ATP (adenosine triphosphate)-breaks down carbsMajor Components:A. GlycolysisB. Krebs CycleC. Electron TransportD. Oxidative
Phosphorylation
Glycolysis
-occurs in cytoplasm-aerobic or anaerobicNet Reaction:Glucose 2 pyruvate2 ATP
Krebs Cycle
- Takes pyruvate- Creates electron
carriers- Produces NADH, FADH2,
NADPH- Occurs in
mitochondria
Electron Transport Chain
- Moves electrons through mitochondrial membrane
- Uses protienaceous electron carriers
- Used to move protons out of mitochonria
- Produces chemical gradient
- Moves ATP synthetase
Oxidative Phosphorylation
- Requires Adenosine Diphosphate (ADP) and inorganic phosphate
- Protons cause ATP Synthase to bond ADP and Pi together