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1 Crop Productivity Partnering Forum | AUTM Partnering Forum AUTM Partnering Forum Crop Productivity Partnering Forum Technologies represented: Updated on 9/12/18 High Value Seeds Technology Title Organization Organization Contact High Oleic, Disease Resistant Peanut Variety Auburn University Brian Wright Potato tuber yield enhancement Iowa State University Dianah Ngonyama Gene controlling spontaneous haploid genome doubling in maize Iowa State University Dianah Ngonyama Haploid Inducing genotype for field and specialty Corn Iowa State University Dianah Ngonyama QTL regulating ear productivity traits in maize Iowa State University Dianah Ngonyama Disease resistant rice Iowa State University Dianah Ngonyama Non-GMO soybean varieties (traits include disease resistance, high yielding, high protein tofu varieties) Iowa State University Dianah Ngonyama Corn germplasm - Includes parent seed; genetic stocks and breeding populations Iowa State University Dianah Ngonyama Popcorn germplasm - Include Inbredlines and populations Iowa State University Dianah Ngonyama High RebD/RebM Stevia varieties Michigan State University Thomas Herlache Maria TM Linda Sweet Cherry Michigan State University Thomas Herlache Redstart' Day-Neutral Strawberry Michigan State University Thomas Herlache Wasatch' Day-Neutral Strawberry Michigan State University Thomas Herlache Osorno Norther Highbush Blueberry Michigan State University Thomas Herlache Mutant Cotton Seed Increases Production and Fiber Quality Texas Tech University David McClure New Guar Gum for Increased Oil Production Texas Tech University David McClure Soybean as a protein biofactory platform to produce epidermal growth factor (EGF) University of Arizona Tod McCauley Enhancing the Sugar, Starch and Cellulose Content in Plants University of Arizona Tod McCauley Increasing the Content of Long Chain Fatty Acids in Seed Oil University of Missouri, St. Louis Tamara Wilgers

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Page 1: AUTM Partnering Forum...2018/09/12  · Improved Alkaline Hydrogen Peroxide Pretreatment of Biomass Michigan State University Thomas Herlache Dynamic Environmental Photosynthetic Imaging

1 Crop Productivity Partnering Forum | AUTM Partnering Forum

AUTM Partnering Forum

Crop Productivity Partnering Forum

Technologies represented: Updated on 9/12/18

High Value Seeds

Technology Title Organization Organization Contact

High Oleic, Disease Resistant Peanut Variety Auburn University Brian Wright

Potato tuber yield enhancement Iowa State University Dianah Ngonyama

Gene controlling spontaneous haploid genome doubling in maize

Iowa State University Dianah Ngonyama

Haploid Inducing genotype for field and specialty Corn

Iowa State University Dianah Ngonyama

QTL regulating ear productivity traits in maize Iowa State University Dianah Ngonyama

Disease resistant rice

Iowa State University Dianah Ngonyama

Non-GMO soybean varieties (traits include disease resistance, high yielding, high protein tofu varieties)

Iowa State University Dianah Ngonyama

Corn germplasm - Includes parent seed; genetic stocks and breeding populations

Iowa State University Dianah Ngonyama

Popcorn germplasm - Include Inbredlines and populations Iowa State University Dianah Ngonyama

High RebD/RebM Stevia varieties Michigan State University Thomas Herlache

MariaTM Linda Sweet Cherry Michigan State University Thomas Herlache

Redstart' Day-Neutral Strawberry Michigan State University Thomas Herlache

Wasatch' Day-Neutral Strawberry Michigan State University Thomas Herlache

Osorno Norther Highbush Blueberry Michigan State University Thomas Herlache

Mutant Cotton Seed Increases Production and Fiber Quality Texas Tech University David McClure

New Guar Gum for Increased Oil Production Texas Tech University David McClure

Soybean as a protein biofactory platform to produce epidermal growth factor (EGF)

University of Arizona Tod McCauley

Enhancing the Sugar, Starch and Cellulose Content in Plants University of Arizona Tod McCauley

Increasing the Content of Long Chain Fatty Acids in Seed Oil University of Missouri, St. Louis Tamara Wilgers

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2 Crop Productivity Partnering Forum | AUTM Partnering Forum

Innovative Chemical Pest Management Solution

Technology Title Organization Organization Contact

Insecticidal Extracts from Hops and Other Plants Michigan State University Thomas Herlache

Anti-mycotoxin compounds Michigan State University Thomas Herlache

Medicinal Plant Compound Reduces Mycotoxins and Sporulation Michigan State University Thomas Herlache

Lignin-Derived Aldehydes as Antifungal Agents Wisconsin Alumni Research Foundation

Emily Bauer

Improved, Robust Rootstock Technology for Pest Management (see pgs. 9 -10) University of Connecticut Ana Fidantsef

Using PDE Inhibitors to Combat Nematode Infestation University of New Hampshire Maria Emanuel

Innovative Biological Pest Management Solution Technology Title Organization Organization Contact

Bacterial strains as probiotic inoculants in agriculture (nematicidal) Auburn University Brian Wright

Use of the XA1 rice gene to confer broad Xanthomonas resistance in plants

Iowa State University Dianah Ngonyama

Insect toxin delivery mediated by a densovirus coat protein

Iowa State University Dianah Ngonyama

Identification of Bt toxins to target Asian Citrus Psyllid for HLB treatment in citrus

Iowa State University Dianah Ngonyama

Foxtail mosaic virus transient gene silencing vector for maize & other monocots

Iowa State University Dianah Ngonyama

miRNA396 as a tool to control cyst nematodes

Iowa State University Dianah Ngonyama

Arabidopsis nonhost resistance genes(s) for engineering soybeans resistant to Sudden Death Syndrome

Iowa State University Dianah Ngonyama

Soybean genes for enhanced Sudden Death Syndrome resistance

Iowa State University Dianah Ngonyama

Modification of the nodulating, nitrogen-fixing soybean symbiont Bradyrhizobium japonicum to increase plant disease resistance

Iowa State University Dianah Ngonyama

Identification and application Arabidopsis nonhost resistance gene(s) in creating disease resistant soybean cultivars

Iowa State University Dianah Ngonyama

Cry3Bb1-resistant strains of western corn rootworm - Hopkinton strain

Iowa State University Dianah Ngonyama

Method and Plants for Improved Production of Terpenoids Michigan State University Thomas Herlache

Soybean resistant to cyst nematodes Southern Illinois University Robert Patino

gdhA Gene Expression to Increase Infection Resistance in Plants

Southern Illinois University Robert Patino

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3 Crop Productivity Partnering Forum | AUTM Partnering Forum

Aflatoxin Free Crops

University of Arizona Tod McCauley

Transgenic Resistance To Cotton Leaf Curl Disease In Cotton

University of Arizona Tod McCauley

Secreted antimicrobial compounds from naturally occurring bacteria inhibit the growth of the of the rice pathogen Burkholderia glumae

University of Arkansas, Div. of Ag. Bryan Renk

Control of Rice pathogenic Fungi with Endophyte Fungi of Genus Trichoderma

University of Arkansas, Div. of Ag. Bryan Renk

Root Extracts with Anti-Fungal Activities University of Arkansas, Div. of Ag. Bryan Renk

Method to mitigate salicylic acid inhibition of crop growth without compromising disease resistance

University of Georgia Research Foundation

Shelley Fincher

Root-knot nematode-resistant plants (copy & paste link in browser: https://flintbox.com/public/project/49430/)

University of Georgia Research Foundation

Shelley Fincher

Method for Enhancing FattyAcid Amide Hydrolase Activity

University of Missouri – Kansas City Tamara Wilgers

Increasing Resistance to Soybean Cyst Nematode Wisconsin Alumni Research Foundation

Emily Bauer

Modulation of NADPH oxidases to confer biotic and abiotic stress tolerance in soybean

Wisconsin Alumni Research Foundation

Emily Bauer

Stress Tolerance Solution Technology Title Organization Organization Contact

Photosynthetic and Heat Stress Trait Improvement (Mendel)

Auburn University Brian Wright

Bacterial strains as probiotic inoculants in agriculture (drought tolerance)

Auburn University Brian Wright

"Xerico" drought tolerance gene Michigan State University Thomas Herlache

Drought tolerance promoters

Michigan State University Thomas Herlache

“Relief of Repression” Strategy to Improve Plant Pest Resistance and Maintain Growth Rate

Michigan State University Thomas Herlache

Use of Arginase or Threonine Deaminase in Plant Protection Against Insects

Michigan State University Thomas Herlache

High-throughput phenotyping platform for screening abiotic stress tolerant crops

Missouri University of Science & Technology

Tamara Wilgers

AtRAVs Make Longer Cotton Fibers Under Drought

Texas Tech University David McClure

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4 Crop Productivity Partnering Forum | AUTM Partnering Forum

Drought Adaptive genes (copy & paste link in browser: https://nutech.flintbox.com/public/project/28630/)

NUtech Ventures Jeewan Jyot

Increasing Salt Tolerance In Plants

University of Arizona Tod McCauley

Method of improving tolerance of plants to herbicides using seed insecticide treatments

University of Arkansas, Div. of Ag. Bryan Renk

Increased Yield and Abiotic Stress Resistance in Plants By Overexpressing Vacuolar Proton Pyrophosphates (see pg. 11)

University of Connecticut Ana Fidantsef

Salt/Drought Tolerance Enhancement in Plants by Overexpressing Vacuolar Proton Pyrophosphatases (see pg. 12)

University of Connecticut Ana Fidantsef

Crop Resistance to Nematodes University of Missouri - Columbia Tamara Wilgers

SAUR gene for improvements of Root System Architecture, Waterlogging, Drought Resistance, and Yield in Soybean

University of Missouri - Columbia Tamara Wilgers

Genes and markers associated with root system architecture, drought tolerance, and yield performance and methods of use

University of Missouri - Columbia Tamara Wilgers

Targeted Modification of Maize Roots to Enhance Abiotic Stress Tolerance Wisconsin Alumni Research Foundation

Emily Bauer

Plant Growth Regulators and Biostimulants Technology Title Organization Organization Contact

Bacterial strains as probiotic inoculants in agriculture (biostimulants) Auburn University Brian Wright

Inexpensive additive and method for increasing plant growth Auburn University Brian Wright

Plant protein and biochar fertilizer Iowa State University Dianah Ngonyama

Genes controlling plant growth Iowa State University Dianah Ngonyama

Enhanced plant growth through mobile RNA signals Iowa State University Dianah Ngonyama

Natural Biostimulant/Adjuvant Compositions for Increased Plant Growth/Biomass, Improved Fungicide and Fertilizer Efficacy, Dew Suppression, and Dormancy-Breaking in Cultivated Turfgrasses

Michigan State University Thomas Herlache

Method of Manipulating Grape Berry Ripening by Small RNAs Texas Tech University David McClure

Use of fungal laccases to facilitate biodethatching University of Georgia Research

Foundation Shelley Fincher

Enzyme compositions and applications to reduce soil water repellency University of Georgia Research Foundation

Shelley Fincher

Soybean root-tissue type and and nodule-tissue type specific promoters

University of Missouri - Columbia Tamara Wilgers

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5 Crop Productivity Partnering Forum | AUTM Partnering Forum

Smarter Phytochrome Engineering for Smaller, Denser Crops Wisconsin Alumni Research Foundation

Emily Bauer

Extending Juvenile Stage of Plants for Biofuels and Feedstock Wisconsin Alumni Research Foundation

Emily Bauer

Precision Agriculture Technology Title Organization Organization Contact

Electrophoretic soil nutrient sensor for agriculture Iowa State University Dianah Ngonyama

Soil nitrate system for precision management of nitrogen fertilizer applications

Iowa State University Dianah Ngonyama

Sensor for in-situ, wireless soil sensing Iowa State University Dianah Ngonyama

Device to determine susceptibility to root lodging Iowa State University Dianah Ngonyama

Rapid, on-site Characterization of Soil Chemical and Physical Properties Texas Tech University David McClure

Variable-Flow Solar Powered Tail-water Recovery Pump System

University of Arkansas, Div. of Ag. Bryan Renk

Fixed Angle Nozzle Body Adaptor

University of Arkansas, Div. of Ag. Bryan Renk

Onion Quality Assessment Using X-Ray Tomography (copy & paste link in browser: https://flintbox.com/public/project/30609/)

University of Georgia Research Foundation

Shelley Fincher

Berry Impact Recording Device (BIRD II) ( copy & paste link in browser: https://flintbox.com/public/project/51326/)

University of Georgia Research Foundation

Shelley Fincher

Software Management Tool for Variable Source Fertilizer Application University of Missouri - Columbia Tamara Wilgers

New imaging software algorithm for fast measurement of yield and quality of corn and other commercially valuable crops

Wisconsin Alumni Research Foundation

Emily Bauer

Hyperspectral reflectance-based models for early disease detection in potato

Wisconsin Alumni Research Foundation

Emily Bauer

Food and Feed Processing Technology Technology Title Organization Organization Contact

A novel vegetable oil-based material as substitute for carnauba wax

Iowa State University Dianah Ngonyama

Lignocellulosic Biomass Pretreatment Using Gaseous Ammonia (AFEX)

Michigan State University Thomas Herlache

Ammonia-based biomass processing (AFEX) to produce highly digestible biomass

Michigan State University Thomas Herlache

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6 Crop Productivity Partnering Forum | AUTM Partnering Forum

AFEX pellets for feed and biofuels

Michigan State University Thomas Herlache

Probiotic Feed Additive for Livestock (copy & paste link in browser:

https://nutech.flintbox.com/public/project/48022/)

NUtech Ventures Jeewan Jyot

Novel probiotic strains having the ability to inhibit growth of Fusobacterium necrophorum and Streptococcus bovis (copy and paste link in browser:

https://nutech.flintbox.com/public/project/31137/ )

NUtech Ventures Jeewan Jyot

Improved Methods for Producing Low-Cost Protein-Polysaccharide Conjugates for Use in Foods and Beverages

Wisconsin Alumni Research Foundation

Emily Bauer

Modification of lignin in plants ( copy & paste link in browser: https://flintbox.com/project/detail/49424/)

University of Georgia Research Foundation

Shelley Fincher

Modification of pectin in plants ( copy & paste link in browser: https://flintbox.com/project/detail/49428/)

University of Georgia Research Foundation

Shelley Fincher

Multiplex assay for detection of bacterial food contaminants University of Missouri - Columbia Tamara Wilgers

Avian beta-defensin analogues as therapeutics and food preservative agents

University of Missouri - Columbia Tamara Wilgers

Other Enabling Technology Technology Title Organization Organization Contact

Method of Protection of Bacterial Inoculants

Auburn University Brian Wright

Transparent soil made by gel beads

Iowa State University Dianah Ngonyama

SSM sequence models

Iowa State University Dianah Ngonyama

Soybean Mosaic Virus as a transient expression vector for soybeans

Iowa State University Dianah Ngonyama

Foxtail Mosaic Virus Transient Gene Silencing Vector for maize & other monocots

Iowa State University Dianah Ngonyama

An efficient DNA-Based Viral Gene Silencing Vector system for soybean functional genomics

Iowa State University Dianah Ngonyama

Metacaspase II for engineering soybean for disease resistance

Iowa State University Dianah Ngonyama

Soybean transformation and regeneration using half-seed explants

Iowa State University Dianah Ngonyama

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7 Crop Productivity Partnering Forum | AUTM Partnering Forum

Midwest adapted haploid inducer for maize

Iowa State University Dianah Ngonyama

Plants over-producing mixed-linkage glucans for forage and biomass feedstock applications

Michigan State University Thomas Herlache

Self-compatible diploid potato lines

Michigan State University Thomas Herlache

Method and Plants for Improved Production of Terpenoids

Michigan State University Thomas Herlache

Diterpene syntesis platform for flavoring and insect repellents

Michigan State University Thomas Herlache

FMT Enzyme Enabling Simplified Plant Processing

Michigan State University Thomas Herlache

Identification of A Grass-Specific Enzyme That Acylates Monolignols with P-Coumarate (PMT)

Michigan State University Thomas Herlache

Enhanced Stability Wrinkled1 For High Oil Production

Michigan State University Thomas Herlache

Wrinkled 1 (wri1) Seed-Oil-Increasing Transcription Factor

Michigan State University Thomas Herlache

Use of Plants with Increased Level of Highly Methylesterified Homogalacturonan for Increased Yield or Improving Digestibility of Plant Biomasses

Michigan State University Thomas Herlache

Control of Cellulose Biosynthesis

Michigan State University Thomas Herlache

A Source and Production Method for Acetyl-Triacylglycerols (ac-TAG synthase gene)

Michigan State University Thomas Herlache

Improved Alkaline Hydrogen Peroxide Pretreatment of Biomass

Michigan State University Thomas Herlache

Dynamic Environmental Photosynthetic Imaging Apparatus and Methods

Michigan State University Thomas Herlache

OLIVER: A Platform for Visualization and Mining of High-Resolution and High-Throughput Data

Michigan State University Thomas Herlache

Method for Creating High Yield Plants Using the K-Domain of a MIKC Type MADS-Box Gene

Michigan State University Thomas Herlache

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8 Crop Productivity Partnering Forum | AUTM Partnering Forum

Subsurface Water Retention Barrier Installation Device (BID) for Installing Contoured Engineered Polyethylene Membranes (CEPEM) in Excessively Drained Soils

Michigan State University Thomas Herlache

Assemblies for use in Evaluating Subsurface Contamination, and Related Methods

Missouri University of Science & Technology

Tamara Wilgers

Technology Platform for Microbial Genome Modification via Transient Gene Inactivation (copy and paste link in browser:

https://nutech.flintbox.com/public/project/28790/)

NUtech Ventures Jeewan Jyot

Development of multiple ALS-resistance sorghum germplasms (copy and paste

link in browser: https://nutech.flintbox.com/public/project/31358/)

NUtech Ventures Jeewan Jyot

Specialized Acyltransferases for Production of Seed Oils With Enhanced Short-/Medium-Chain Fatty Acid Content (copy and paste link in browser:

https://nutech.flintbox.com/public/project/29057/)

NUtech Ventures Jeewan Jyot

Method for Production of Soybean Oil with High Saturated and Low Poly Unsaturated Fatty Acid Profile (copy and paste link in browser:

https://nutech.flintbox.com/public/project/28632/)

NUtech Ventures Jeewan Jyot

Multi-band Photodiode sensor (plant canopy temperature and multi-spectral measurements)

Southern Illinois University Robert Patino

A Method for Stacking Genes into a Single Genomic Site Through Iterative Genetic Transformation of Plants

University of Arkansas, Div. of Ag. Bryan Renk

Cornstalk Nitrate Cutter

University of Arkansas, Div. of Ag. Bryan Renk

Microbial Carriers for Soil Remediation and Agriculture (see pgs. 13-14) University of Connecticut Ana Fidantsef

Soil Micromodel for Studying Soil Properties (see pgs. 15-16) University of Connecticut Ana Fidantsef

Increased Seed Production by Overexpressing Vacuolar Pyrophosphatases in the Pollen (see pg. 17)

University of Connecticut Ana Fidantsef

Use of transposable elements ( copy & paste link in browser: https://flintbox.com/public/project/49427/)

University of Georgia Research Foundation

Shelley Fincher

Increasing phenotypic variability for plant breeding copy & paste link in browser: https://flintbox.com/project/detail/49543/)

University of Georgia Research Foundation

Shelley Fincher

Gene for induction of parthenogenesis, a component of apomictic reproduction

University of Georgia Research Foundation

Shelley Fincher

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IMPROVED, ROBUST ROOTSTOCK TECHNOLOGY FOR PEST MANAGEMENT

innovation.uconn.edu

Background: Plant grafting is an important technique for horticultural and silvicultural production. Rootstocks that are more resistant to soil borne pathogens and/or abiotic stresses are routinely used for grafting with scions (stem portion) of commercial interest. In the grafting process, many rootstock plants suffer from undesirable lateral bud outgrowth, low grafting success rates or poor rooting, making the process quite expensive. Despite the costs, over 70 woody perennial crop species propagated for their edible fruits are grown on rootstocks. Furthermore, 20 of the 25 most-produced fruit and nut crops are grafted. Vegetable grafting is also becoming increasingly popular in the US.

The Technology: Elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting UConn researchers have developed a technology that uses root selective expression of tryptophan-2-monooxygenase gene (iaaM) and CKX (a cytokinin degradation gene) to increase auxin levels and decrease cytokinins. These rootstocks displayed inhibited lateral bud outgrowth, improved root initiation and elongation, and biomass and did not disrupt normal growth and developmental patterns in wild-type scions. These rootstock plants did not present common characteristics present in rootstocks: undesirable lateral bud outgrowth, low grafting success rates, or poor rooting. On the contrary, these plants present enhanced grafting success rate, and since they are more robust, they might be more tolerant/resistant against diseases that attack the roots. Technology is particularly of interest against citrus greening, since the disease can attack the root system of the plants before symptoms are visible. Since rootstocks containing this technology are healthier and more robust, they might provide an enhanced barrier to this devastating disease. Commercial Applications:

• Applicable for economically important orchard woody plants, including apples, pears, grapes, coffee, and walnuts, vegetables and nurseries (roses and others)

• Technology might improve resistance against citrus greening disease

Advantages:

• More robust, healthier rootstock • Improved grafting success rate • Eliminates labor, time, and cost of chemical or manual removal of lateral buds • Combines nontransgenic scions with transgenic rootstock; therefore fruits, seeds and pollen grains produced

from scion shoots are nontransgenic

Stage of Development:

• Technology has been demonstrated in tobacco in the greenhouse • Technology in the process of being tested in citrus for tolerance/resistance against citrus greening disease

Intellectual Property Information:

• Filed non-provisional patent application 5/21/2018

Related Publication: Li, W., et al., Elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting success of use, 2017, Plant Biotechnology Journal, pp. 1–10.

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IMPROVED, ROBUST ROOTSTOCK TECHNOLOGY FOR PEST MANAGEMENT

innovation.uconn.edu

Inventor: Yi Li, Ph.D.

Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected] Vaibhav Saini, Ph.D. Licensing Director (860) 679-7894 [email protected]

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INCREASED YIELD AND ABIOTIC STRESS RESISTANCE IN PLANTS BY OVEREXPRESSING VACUOLAR PROTON PYROPHOSPHATASES

innovation.uconn.edu

Background: The prospects for feeding humanity are formidable. There is a constant need to obtain plants with higher yields and abiotic/biotic stress resistance.

The Technology: Plant cells and plants overexpressing vacuolar proton pyrophosphatases UConn researchers have engineered tobacco and petunia transgenic plants to overexpress Arabidopsis thaliana vacuolar pyrophosphatases AVP-1. These plants showed increased meristematic activity resulting in larger leaves, stem, flower, fruit, root structures, increased salt tolerance, enhanced drought and freeze tolerance. Commercial Applications:

• Technology can be used in crop plants, ornamental plants, grasses, turf grass, and shrubs

Advantages:

• Increased yield and salt tolerance, enhanced drought and freeze tolerance, and larger plant size • Cheaper production cost due to higher yield

Stage of Development:

• Demonstrated in tobacco and petunia

Intellectual Property Information:

• Issued Patent No.: US 8,003,852; issued on 08-23-2011 • Application No.: US7534933B2

Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected] Vaibhav Saini, Ph.D. Licensing Director [email protected]

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INCREASED SEED PRODUCTION BY OVEREXPRESSING VACUOLAR PYROPHOSPHATASES IN THE POLLEN

innovation.uconn.edu

Background: The prospects for feeding humanity are formidable. There is a constant need to obtain plants with higher yields, including mass or seed yield, and abiotic/biotic stress resistance. Seed yield might be increased with application of growth factors. However, this is costly and time consuming, and does not necessarily substantially increase the yield of seeds from the plants. There is a constant need for developing an effective method for increasing the yield of seeds from plants.

The Technology: Transgenic pollen expressing exogenous plant vacuolar pyrophosphatase UConn researchers have engineered tobacco transgenic plants to constitutively overexpress Arabidopsis thaliana vacuolar pyrophosphatases AVP-1. These plants showed increased resistance to stresses such as drought, extended exposure to subfreezing temperatures, and high salinity. Furthermore, seed yield produced by these plants was dramatically higher than the control plants in both number of seeds and seed pod mass. Commercial Applications:

• Crop plants produced for seed consumption such as rice, canola, wheat, corn and sunflower

Advantages:

• Dramatically increased seed yield, increased salt and drought tolerance, increased freeze tolerance • Cheaper production cost due to higher yield

Stage of Development:

• Demonstrated in tobacco

Intellectual Property Information:

• Issued Patent No.: US 8,168,864; issued on 05-01-2012

Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected] Vaibhav Saini, Ph.D. Licensing Director (860) 679-7894 [email protected]

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SALT/DROUGHT TOLERANCE ENHANCEMENT IN PLANTS BY

OVEREXPRESSING VACUOLAR PROTON PYROPHOSPHATASES

innovation.uconn.edu

Background: Artificial irrigation is widely used in crop production since dry areas usually offer optimal photoperiod and temperature conditions for the growth of most crops. Irrigation has solved the problem in the short term in these areas since water supplies always contain some dissolved salt, which upon evaporation gradually accumulates on the soils. Therefore, in the long term, these soils become unusable for agriculture. There is also a problem of runoff of chemicals due to aggressive fertilization in soils with limited nutrients to the plants, especially phosphates (Pi). Thus, there is a growing and unmet need for crops to have increased tolerance to salts and Pi.

The Technology: Plant cells and plants overexpressing vacuolar proton pyrophosphatases UConn researchers have demonstrated that by transforming Arabidopsis, tomato and rice with a plant vacuolar pyrophosphatase, there was increased tolerance to one or more salts, increased yield, larger plant size and increased Pi uptake under Pi-sufficient and Pi-deficient growth conditions in the transgenic plant. For example, data with rice showed that by transforming rice with the rice homologue vacuolar pyrophosphatase, the transgenic line exhibited sustained shoot growth under Pi-deficient (10 µM) conditions, whereas the controls grew poorly; the transgenic line tested developed more robust root systems than controls in both Pi-sufficient (1mM) and Pi-deficient (10 µM) conditions; and the dry plant biomass data confirmed that the transgenic line grown under limiting Pi conditions developed larger roots (90%,P<0.01) and shoots (50%, P=0.01) than controls. Commercial Applications:

• Technology can be used in crop plants, ornamental plants, grasses, turf grass, and shrubs in elevated salt and Pi-sufficient and Pi-deficient conditions

Advantages:

• Increased yield, larger plant size and increased Pi uptake under Pi-sufficient and Pi-deficient growth conditions

• Cheaper production cost due to higher yield

Stage of Development:

• Demonstrated in Arabidopsis, tomato, and rice

Intellectual Property Information:

• Issued Patent No.: 8,058,515; issued on 11-15-2011 • Issued Patent No.: US 8,697.950 B2; issued on 04-15-2014

Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected] Vaibhav Saini, Ph.D. Licensing Director [email protected]

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MICROBIAL CARRIERS FOR SOIL REMEDIATION AND AGRICULTURE

innovation.uconn.edu

Background: The rhizosphere is the zone of soil surrounding plant roots that is under the influence of the root. Rhizosphere associated bacteria can promote plant growth through a variety of mechanisms, for example, by increasing nutrient acquisition (N2 fixation). The primary weakness of conventional technologies that deliver bacteria to roots is the failure to deliver enough functional bacteria to the critical locations within the rhizosphere. Beneficial bacteria added in seed coatings often fail to survive and proliferate and/or fail to colonize growing roots. Furthermore, even if biocontrol bacteria do attach to an emerging root, seedling roots grow very quickly from the leading edge, such that beneficial bacteria cannot reproduce fast enough and become dilute at the growing root tip. Thus, there is an unmet need to more efficiently deliver beneficial bacteria to roots in actively-growing plants.

The Technology: Use of protists to assist movement of rhizobacteria closer to the roots UConn researchers have developed a suitable carrier protist-facilitated transport of plant growth-promoting rhizobacteria as well as bacteria-sized particles in the soil. Protist-facilitated transport in soil may enhance important ecosystem services provided by bacteria, which encompass both remediation and agriculture. Results show higher nodulation in roots, indirectly increasing shoot weight. Furthermore, the technology allows the bacteria to proliferate and move towards rapid growing roots for colonization. Dramatic yield increase has been obtained using the technology in wheat and bean plants. Commercial Applications:

• Any plants where bacteria is used as a fertilizer • Technology can be used in seeds, seed coats, granular carriers, liquid slurry carriers, and liquid suspension

carriers • Technology can be delivered in the soil where the seed will be sown or plant will be planted • Sustainable agricultural productivity via targeted delivery of biocontrol bacteria or encapsulated

agrochemicals • In situ bioremediation: non-invasive means of delivery of key bacterial strains to the soil

Advantages:

• Technology delivers functional bacteria to the critical locations surrounding the roots • Technology allows increase in nodulation, which fixes N2 • Technology reduces need of fertilizers • Increase in shoot mass, improvement in agriculture

Stage of Development:

• Demonstrated in bean and wheat

Intellectual Property Information:

• Patent No.: US9603368B2

Inventors: Daniel Gage, Ph.D. Leslie Shor, Ph.D.

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MICROBIAL CARRIERS FOR SOIL REMEDIATION AND AGRICULTURE

innovation.uconn.edu

Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected] Vaibhav Saini, Ph.D. Licensing Director (860) 679-7894 [email protected]

Page 16: AUTM Partnering Forum...2018/09/12  · Improved Alkaline Hydrogen Peroxide Pretreatment of Biomass Michigan State University Thomas Herlache Dynamic Environmental Photosynthetic Imaging

SOIL MICROMODEL FOR STUDYING SOIL PROPERTIES

innovation.uconn.edu

Background: Global climate change is predicted to increase the occurrence of extreme events such as powerful storms and prolonged droughts. Such extreme events can lead to widespread reduction in soil moisture. Water supplies are also becoming scarcer which have direct implications for sustainable food, feed, and fiber production. The nitrogen-fixing bacteria Sinorhizobium meliloti is commonly found in soils and naturally produces extracellular polymeric substances (EPS). EPS bacteria may allow plants to remain hydrated even with longer rain return intervals, which is very desirable when water in soils is becoming scarcer. There is a constant need for optimization of water usage, which can ultimately enable growers to obtain higher yields and better quality crops.

The Technology: Soil-emulating device which consists of soil, a control chamber, and a microscope UConn researchers have developed a soil-emulating device and a system for evaluation of soil-related properties. The soil-emulating apparatus includes soil micromodel(s) which consists of soil particles with a particle size distribution and a soil structure of a desired soil type. The soil micromodel is cast in a way that provides visualization of air infiltration into pores of the micromodel. The soil micromodel is located within an environmental control chamber to control humidity and can be saturated with a bacterial strain. A microscope can be included to the device. This device allows studies on the interaction of different strains of EPS bacteria and water retention in different kinds of soil. Commercial Applications:

• Tool for studying soil properties and soil ecology for Agriculture improvement and soil bioremediation

Advantages:

• Technology allows that images are captured of the soil micromodel over time • Any soil can be simulated using the device • Convenient way to do study the interactions between physical soil structure and biological processes • Device ultimately allows the optimization of water usage improving Agriculture

Stage of Development:

• Demonstrated in the laboratory

Intellectual Property Information:

• Patent No.: US20160153954A1

Inventors: Daniel Gage, Ph.D. Leslie Shor, Ph.D. Contacts: Ana Lena Fidantsef, Ph.D., MBA Technology Licensing Associate [email protected]

Page 17: AUTM Partnering Forum...2018/09/12  · Improved Alkaline Hydrogen Peroxide Pretreatment of Biomass Michigan State University Thomas Herlache Dynamic Environmental Photosynthetic Imaging

SOIL MICROMODEL FOR STUDYING SOIL PROPERTIES

innovation.uconn.edu

Vaibhav Saini, Ph.D. Licensing Director (860) 679-7894 [email protected]