Upload
phungliem
View
221
Download
2
Embed Size (px)
Citation preview
1ASU Confidential Proprietary
ATP3: A Platform for Engagement and Access to Industry, National Lab, and Academic Expertise, and
World-class Algal R&D Facilities
John A. McGowen, PhD, PMP
Director of Operations and Program Management
Arizona Center For Algae Technology and Innovation
Arizona State University
Bio World Congress 2015 Montreal
July 22, 2015
The Arizona Center for Algae Technology and Innovation (AzCATI): formed
in 2010 through stimulus funds designated by the Science Foundation of
Arizona to serve as a hub for research, testing, and commercialization of
algae-based technologies and products.
2
Arizona Center for Algae Technology and Innovation
• Connect
• Advance
• Collaborate
• Educate
• Launch
3
AzCATI/ASU Algae Portfolio
AzCATI – $4M. CO2 mitigation from flue gas into algae
based fuels; Reactor development; Strain selection and
development; Processes for products; Wastewater;
Downstream processing and nutrient/media recycling; Test
bed expansion
ARPA-e – $7M. Cyano-bacterial based photosynthetic
factories - secrete fatty acids for fuel production
USDA – $1M. Development of best management practices
for algal crop protection
Bioenergy
Technologies
Office
SABC – $ 7.5M. Biochemical conversion of algae to fuels;
QA/QC protocols & characterization; Enzymatic
pretreatment for fuels
DOE – $0.5m. Managing microbial ecology in
cultivation systems
REAP - $6.2M. “Realization of Algae Potential”:
ATP3 – $17M. National algae test bed network
4
Project Overview:
ATP3 national open test bedThe formation of the Algae Testbed Public-Private Partnership leveraged the
existing resources at AzCATI and our partner sites. The network represents
a collaboration of industry, laboratory, and educational facilities across
nation. ATP3 aims to convene all algae stakeholders to facilitate
opportunities and progress more rapidly to commercialization.
4
Cellana
Cal PolyFlorida Algae
AzCATI
GTech
ATP3 partners
5
6
Collaborative Open Testbeds
• Form a national network
• Provide access to stakeholders
• Accelerate R&D outcomes
Collect and Distribute High Impact Data
• Unified research programs
• Pipeline for collection of high-quality
cultivation data to support algae
computational modeling including
biomass productivity, techno-
economic, and life cycle assessment.
• Make data available publically
ATP3 Primary Objectives
ATP3: Testbeds Open for Business
7
• Project Activities: biomass supply (1kg-100’s kg), equipment testing, analytical testing, culture maintenance, cultivation scale up, GM trials, and consultation services to academia, industry and national labs
• Project Categories: fee-for service activities, sponsored research, and subsidized projects through ATP3
Support Program
• Project Benefits: access to facilities to drive technology R&D, de-risk and validate technological innovations
Variety of independent and vertically integrated downstream harvesting unit ops
Provide service to ATP3 customers
- Produce algal biomass in the form of
slurry, paste and dry powers
- Serve as baseline technologies for
the improvement of future
harvesting/dewatering and oil
extraction processes (Valicor and
OpenAlgae platforms)
Support DOE’s TEA, sustainability,
and resource modeling
- Generate validation data for
current/future BETO pathways
- Provide more options to generate
data on the selection of harvesting
methods
- Provide feedstock for HTL, lipid
extraction and other downstream
process/product development
applications
Collaborative Open Testbeds:
Site Access and Customer Management
10
An Example: toll biomass production and pilot scale harvesting/dewatering
Supporting early stage product development- Using the Helix™ PBR as semicontinuous seed platform
supporting larger scale cultivation in open pond raceways (2 x
60 m2) of testbed client supplied proprietary strain- 8 week growth campaign generating 7-8 kg of biomass per batch
(media recycle employed)
- Total project duration 3 months (included indoor scale up)
- Production data generated through harvest can be utilized for
TEA/LCA (with permission from client)
- Biomass delivered to client as whole, freeze dried product and was
used for downstream process validation and generation of
customer samples
Helix Seed Production 60 m2 cultivation campaign
11
Example Pilot Scale PBR/Process Validation Project: Blue Ocean NutraSciences
• Leveraging ATP3 expertise, capabilities, and facilities- Blue Ocean working with the AzCATI testbed site to validate
current production process technology (strain and PBR system)
- First phase (6-9 mo)
- small scale validation of previous work performed for Blue
Ocean at another institution
- 250 gallon scale - includes small scale product production
for customer evaluations
- Second phase
- Fully scoped (contingent on successful first phase)
- Larger scale implementation (up to 3000 gallons)
- Validation of production process and product specifications
- Standard boilerplate for NDA and the service agreement were
negotiated and in place in ~3 weeks.
- Full pay to play project
- Can transition P1-P2 without need for revisiting service
agreement
Education & Training Workshops
• ATP3 has hosted 9 quarterly educational workshops
• Week-long workshop
• Over 30 lecture modules
• Over 15 hands-on field site and laboratory activities
• Well attended by broad mix of academic and industrial participants
• More than 200 participants representing >80 different organizations
• 35% Foreign participants
• Most recent workshop: “Principles and Processes: Algae Culture Maintenance, Production and Downstream Processing” held in May 2015 at NREL and CSM in Golden, Colorado
• Next workshop to be held in Mesa, Arizona November 2015
12
Open Collaborative Testbed: Summary
• The ATP3 testbed network is up and running
• Projects with 32 customers in 2 years (majority focused on non-fuel applications)
• Streamlined business processes – can be applied to multiple sites
- Projects can get going very rapidly
• Ongoing/scheduled projects – come join us! - Cultivation trails (including GMO), analytical support, biomass supply (10’s-100’s kg),
equipment trials, access to facilities to conduct your own R&D, training, etc.- Non-US based companies leveraging the network with a landing pad in the US- Active engagement/access across network for collaboration – FOA’s, SBIR/STTR’s
• ATP3 support program- Opportunity for maximum leverage of DOE investment across underfunded
academics/entrepreneurs- Great path to scale-up bench scale/lab results/outdoor validation- Novel process ideas, strains (including GMO)- New synergies/new collaborations leading to purposeful expansion of the network- Early teaming/results in pursuit of new funding opportunities
• Education and Training workshops have reached approximately 80 different agencies, organizations, companies and academic institutions
- Exploring possibility for certificate programs (CEC’s, etc.) - Offering additional training partner sites with unique capabilities in the near future- Being prepared for the algal economy requires access to a trained workforce
13
High Impact Data:
Long Term Algal Cultivation Trials
ATP3 sets standards and conducts harmonized, rigorous, and objective
long term cultivation trials to provide a realistic assessment of the
state of technology for algal based biofuels and bioproducts.
14
• Our Unified Field Studies (UFS) at the testbed
sites along with our Advanced Field Studies (AFS)
enable comparison of promising production
strains at meaningful scale across variable
conditions
• Our Scientific Data Management System and
validated, harmonized SOP’s for analytical and
production processes ensures data integrity
across all sites
• Our protocols and data from the UFS and AFS are
publicly available and provide a critical resource to
TEA and LCA analysis yielding high impact,
validated data
15
Standardization of processes and systems is
key to executing meaningful multi-site
cultivation trials
16
! ! !FAME!Quantification!LAP!v.!September!28th,!2013!
!
!
1!
!
!
!
!
Determination*of*Total*Lipids*as*Fatty*
Acid*Methyl*Esters*(FAME)*by*in#situ*
Transesterification*
S.!Van!Wychen,!L.!ML.!Laurens!
Laboratory!Analytical!Procedure!(LAP)!Issue!Date:!September!28th,!2013!
! !
!
Laboratory!Analytical!Procedure!
mean sd RSD NAsh 18.8 1.2 6.4 237
Protein 28.2 0.8 2.7 153FAME Lipids 11.6 1.2 10.6 191
Carbohydrates 7.7 1 12.5 198
Setting Standards:
Analytical and Production Methods
17
The ATP3 Framework: systems, tools, data, models and projections
ATP3 is using a harmonized, experimental framework to implement Unified Field Studies that enable comparison of promising strains at a standard system scale.
ATP3 has developed and implemented a pipeline for the collection and distribution of high-quality cultivation data to support algae computational modeling including biomass productivity, techno-economic, and life cycle assessment and making this data publically available.
, , L
M R
BB t P B P B t
t V
x x
max 1 2 3 4 5( ) ( ) ( ) ( ) ( )P P f f I f T f S f pH Day 12 observed
ASU
Avg
ASU
Avg
ASU
Avg
Cel
l Avg
Cel
l Avg
Cel
l Avg
CP
Avg
CP
Avg C
P A
vg
FA A
vg
FA A
vg
FA A
vg
GT
Avg
GT
Avg
GT
Avg
0.00%
1.00%
2.00%
3.00%
4.00%
5.00%
6.00%
7.00%
8.00%
9.00%
Total Pond Down Time Crash Pond Down Time System or Human FailurePond Down Time
% o
f P
on
d D
ays
Pond Down Time by Site
Harvest Yield Productivity Data
Nannochloropsis oceanica KA32
Clear seasonal effect at every site with distinctive variations
Harvest Period
Public Access to Data
Using the existing infrastructure and expertise of OpenEI.org provides a rapid, robust, and low-cost solution for making the ATP3
datasets public. Curated 2014 data summaries and raw data to be posted in Q4 FY’15.
http://en.openei.org/search/#q=atp3
Policy
Support from USDA for algae as precision agriculture
EPA and USDA collaboration critical on CO2 and GMO policies, crop designation, agricultural practices and policies and tax incentives/rebates.
Carbon reuse and EPA rule-making: recycle waste rather than bury waste 20
Steady and encouraging progress
Challenge Space: 1. High cost of production
2. Demonstrating scalability and
3. Availability/cost of resources (nutrients,
carbon, water, etc.)Opportunity Space:
Technical
Increase productivity
Increase lipid content
Increase robustness and resiliency to resist predators
Improve early detection of contaminants
Develop new strains to handle high salinity
Improve energy efficiency of downstream processing
Business
Take advantage of high value markets to facilitate learning
Continue to focus on multiple technology pathways and strategies
Leverage co-location, waste streams to produce clean water/energy savings as a revenue stream/cost offset
Foster business innovation
Summary
ATP3 has established validated framework for implementing rigorous, long-term multi-site cultivation trials including standard methods for biomass and bioproduct productivity assessments
Allows determination of the effects of regional, seasonal, environmental variation that is to be expected for a national (international) deployment of algae cultivation
Experimental program has expanded to include larger scale, more cultivars, nutrient sourcing, media recycle and other additional capabilities of the partner sites
Critical validation data source for biomass productivity modeling, TEA, LCA and RA community - will allow for refinement of the current state of technology (SOT) assessments utilized by DOE and the broader industry/investment community
Challenges remain, but access to expertise and leveraging existing investment in facilities can help to accelerate algae R&D, de-risk technology, accelerate commercialization
21
Acknowledgements
Florida Algae Steven SchlosserChris WithstandleyMary RiddleNancy Pham Ho (FIT)
CellanaValerie HarmonMartin SabarskyEmily KnurekKate EvansPeter PrentissReyna JavarKari WolffKeao Bishop-YuanLynn GriswoldChristina BoykoCharlie O’Kelley
ASUGary DirksJohn McGowenThomas DempsterPete LammersMilt SommerfeldWilliam BrandtJessica ChengJordan McAllisterSarah ArrowsmithDavid CardelloTheresa RosovMary CuevasJeffrey PrairieRichard MalloyXuezhi ZhangHenri GerkenPierre WenselLinda BoedekerSarah MasonTravis JohnsonSydney Lines
UTEXSchonna ManningJerry BrandValicor RenewablesKiran KadamBrian Goodall
NRELPhil PienkosLieve LaurensEd WolfrumDavid CrockerRyan DavisStefanie Van WychenEric KnoshaugSandia National LabsRon PateTodd LanePatricia GharagozlooThomas ReichardtJessica DrewryPamela LaneCal PolyTryg LundquistBraden CroweEric NicolaiCommercial Algae ManagementAlbert VitaleRobert VitaleGeorgia TechYongsheng ChenSteven Van GinkelThomas IgouZixuan Hu
ASU UndergradsWyatt WesternMariah PattonMaria BautistaCarlos LunaDelaney De HertoghShaylin McgheeCaden Offield
GT UndergradsFariha HassanJerry DuncanFrazier WoodruffShusuke DoiHao FuPatricia Penalver-ArguesoAllison Dunbar
Allison CarrSichoon ParkPriya PradeepTerry SnellCatherine AchukwuChristine Yi
CP UndergradsAydee MelgarGulce OzturkKaitlyn JonesMichael AntoineTrung K TranJake BenderHeather FreedDaniel McBroomMichele Hendrickson
Gerard NguyenDeven DilibertoJack SunderlandDan AverbujAnn Marie SequeiraLauren MillerMichele HendricksonEmily WangJack SunderlandAnn Marie SequeiraSoroush AboutalebiLauren MillerSamantha LuiMichele HendricksonGabriella CamposWill BrilesLetty Thottathil
23ASU Confidential Proprietary
The Goal – encourage and enable small businesses, entrepreneurs and underfunded academic
researchers to pursue new approaches to solving technical issues associated with
commercialization of algae biofuels, bioproducts, processes.
What is it ?
- Access to laboratory, outdoor facilities and resource support for novel projects
- ATP3 provides subsidized access to testbed facilities, technical expertise, and M&S
- Preference for support will be given to: • Short-term projects ranging from 1-3 months (typical project target $10K-$30K)
• Researchers willing to share data and results widely through publication
• Projects that leverage on-going activities already occurring (e.g., cultivation trials)
Easy to start the process: Visit ATP3.org and fill out an expression of interest form
Support may include:
Biomass (whole, extracted, oil)
Access to cultivation & downstream
equipment (eq. transport, install/removal)
Access to R&D, production & analytical
expertise
Access to laboratory and office space
Travel stipends to testbed (currently limited to
academic clients)
Initial cohort of support projects includes:
Novel cultivars for flue gas capture (Univ. of Del.)
Carbon management and delivery (LBNL)
AD with LEA (Cal Poly)
Novel harvesting with VAL (Searen)
Metabolic pH control for enhanced CO2 utilization
(Penn State)
Initial cohort of support projects includes:
Accepting applications for next cohort selection
ATP3 Offers Support Program
24